The nucleotide sequence of a rat myosin light chain 2 gene (1984)
Nudel, U., Calvo, J.M., Shani, M., Levy, Z.
A rat myosin light chain 2 gene was characterized by nucleotide sequence and S1 mapping analyses. It contains seven exons separated by six introns. The corresponding mRNA is predicted to be 654...
Tesis presentada para optar al Grado de Doctor en Filosofía y Ciencias de la Educación
Braaten, B A, Platko, J V, Van Der Woude, M W, Simons, B H, De Graaf, F K, Calvo, J M, ...
The methylation blocking factor gene (mbf) in Escherichia coli is required for specific methylation inhibition of two DNA GATC sites upstream of the papBA pilin promoter and transcriptional...
The amino acid sequence of Lrp is highly conserved in four enteric microorganisms.
Friedberg, D, Platko, J V, Tyler, B, Calvo, J M
Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other...
A consensus sequence for binding of Lrp to DNA.
Cui, Y, Wang, Q, Stormo, G D, Calvo, J M
Lrp (leucine-responsive regulatory protein) is a major regulatory protein involved in the expression of numerous operons in Escherichia coli. For ilvIH, one of the operons positively regulated by...
Effects of nutrition and growth rate on Lrp levels in Escherichia coli.
Landgraf, J R, Wu, J, Calvo, J M
Lrp (leucine-responsive regulatory protein) activates some Escherichia coli operons that function in anabolism and represses others involved in catabolism (for a review, see J. M. Calvo and R. G....
Lrp is a regulatory protein in Escherichia coli that increases expression of some operons and decreases expression of others. Mutations in Lrp were isolated on the basis of their effects on ilvIH,...
Regulation of the Escherichia coli lrp gene.
Wang, Q, Wu, J, Friedberg, D, Plakto, J, Calvo, J M
Lrp (leucine-responsive regulatory protein) is a major Escherichia coli regulatory protein which regulates expression of a number of operons, some negatively and some positively. This work relates to...
Haney, S A, Platko, J V, Oxender, D L, Calvo, J M
We investigated the relationship between two regulatory genes, livR and lrp, that map near min 20 on the Escherichia coli chromosome. livR was identified earlier as a regulatory gene affecting...
Transcriptional regulation of the ilv-leu operon of Bacillus subtilis.
Grandoni, J A, Zahler, S A, Calvo, J M
We used primer extension and mutational analysis to identify a promoter upstream of ilvB, the first gene in the ilv-leu operon of Bacillus subtilis. Between the promoter and ilvB, there is a 482-bp...
Regions of the Bacillus subtilis ilv-leu operon involved in regulation by leucine.
Grandoni, J A, Fulmer, S B, Brizzio, V, Zahler, S A, Calvo, J M
The ilv-leu operon of Bacillus subtilis is regulated in part by transcription attenuation. The cis-acting elements required for regulation by leucine lie within a 683-bp fragment of DNA from the...
Bartkus, J M, Tyler, B, Calvo, J M
Four adjacent Leu codons within the leu leader RNA are critically important in transcription attenuation-mediated control of leu operon expression in Salmonella typhimurium and Escherichia coli (P....
In vitro transcription from the Escherichia coli ilvIH promoter.
Lrp (leucine-responsive regulatory protein) activates the expression of the Escherichia coli ilvIH operon in vivo and mediates the repression of the operon by exogenous leucine. In previous studies,...
A protein that binds to the regulatory region of the Escherichia coli ilvIH operon.
Ricca, E, Aker, D A, Calvo, J M
The ilvIH operon of Escherichia coli encodes acetohydroxyacid synthase III, an isoenzyme involved in branched-chain amino acid biosynthesis. Transcription of the ilvIH operon is repressed by growing...
The ilvIH operon of Escherichia coli is positively regulated.
Platko, J V, Willins, D A, Calvo, J M
The ilvIH operon of Escherichia coli (located near min 2) encodes acetohydroxyacid synthase III, an isozyme involved in branched-chain amino acid biosynthesis. A strain with lacZ fused to the ilvIH...
Escherichia coli B/r leuK mutant lacking pseudouridine synthase I activity.
Searles, L L, Jones, J W, Fournier, M J, Grambow, N, Tyler, B, Calvo, J M
Escherichia coli B/r strain EB146 containing mutation leuK16 has elevated levels of enzymes involved in the synthesis of leucine, valine, isoleucine, histidine, and tryptophan (Brown et al., J....
Haughn, G W, Wessler, S R, Gemmill, R M, Calvo, J M
The nucleotide sequence of over 800 base pairs of DNA upstream of leuP was determined for Escherichia coli and Salmonella typhimurium. In both of these enteric bacteria, approximately 500 base pairs...
Promoter mutation causing catabolite repression of the Salmonella typhimurium leucine operon.
Gemmill, R M, Tripp, M, Friedman, S B, Calvo, J M
Two mutations that affect expression of the Salmonella typhimurium leu operon were investigated. leu operon DNA from these mutant strains was cloned, and nucleotide sequences of the leu control...
Carter, P W, Weiss, D L, Weith, H L, Calvo, J M
In Salmonella typhimurium, expression of the leucine operon is regulated by a transcription attenuation mechanism. According to a current model of attenuation, elevated expression of this operon...
Physical characterization of the ilvHI operon of Escherichia coli K-12.
Squires, C H, De Felice, M, Wessler, S R, Calvo, J M
The ilvHI and leu genes of Escherichia coli K-12 are contained on a single 10.9-kilobase EcoRI fragment of deoxyribonucleic acid derived from the leu transducing phage lambda G4. Since the expression...
IlvHI locus of Salmonella typhimurium.
Squires, C H, De Felice, M, Lago, C T, Calvo, J M
In Escherichia coli K-12, the ilvHI locus codes for one of two acetohydroxy acid synthase isoenzymes. A region of the Salmonella typhimurium genome adjacent to the leucine operon was cloned on...
Unusual organization of the ilvIH promoter of Escherichia coli.
Haughn, G W, Squires, C H, De Felice, M, Largo, C T, Calvo, J M
Analysis of plasmids containing ilvIH-galK fusions indicated that the Escherichia coli ilvIH promoter and sequences sufficient to cause leucine repression lie within 363 base pairs (bp) of ilvI....
Isolation and characterization of lambda pleu bacteriophages.
In the Escherichia coli lysogen HfrH73 described by Shimada et al. (1973), none of the enzymes coded for by the leucine operon is synthesized due to an insertion of phage lambda into cistron leuA....
Distribution of the Isopropylmalate Pathway to Leucine Among Diverse Bacteria
Stieglitz, B. I., Calvo, J. M.
α-Isopropylmalate synthase and β-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: Chromatium D, Rhodopseudomonas spheroides, Hydrogenomonas H16,...
Effect of 4-Azaleucine upon Leucine Metabolism in Salmonella typhimurium1
dl-4-Azaleucine (5 × 3−3m) added to exponentially growing cells of Salmonella typhimurium resulted in an abrupt cessation of growth lasting 4 to 8 hr followed by a resumption of division. The...
Recognition of an Escherichia Operator by a Salmonella Repressor
Brandriss, Marjorie C., Calvo, J. M.
Escherichia coli leu+ episomes were transferred to Salmonella typhimurium. The levels of two leucine-forming enzymes were normal (low) in the resulting merodiploids, suggesting that the putative...
Mutants of Salmonella typhimurium with an Altered Leucyl-Transfer Ribonucleic Acid Synthetase
Alexander, Renee R., Calvo, J. M., Freundlich, M.
Two trifluoroleucine-resistant mutants of Salmonella typhimurium, strains CV69 and CV117, had an altered leucyl-transfer ribonucleic acid (tRNA) synthetase. The mutant enzymes had higher apparent Km...
Salmonella Locus Affecting Phosphoenolpyruvate Synthase Activity Identified by a Deletion Analysis
Calvo, J. M., Goodman, M., Salgo, M., Capes, N.
Strain leu-4017, derived from Salmonella typhimurium LT2, cannot utilize acetate, pyruvate, or citric acid cycle intermediates as sole sources of carbon. The mutation in this strain extends from the...
Calvo, J. M., Freundlich, M., Umbarger, H. E.
5′,5′,5′-Trifluoro-dl-leucine inhibited the activity of α-isopropylmalate synthetase (the initial enzyme unique to leucine biosynthesis) as well as the growth of Salmonella typhimurium....
A large family of bacterial activator proteins.
Henikoff, S, Haughn, G W, Calvo, J M, Wallace, J C
At least nine different bacterial proteins belong to the LysR family. The gene sequence for one of these proteins is presented here. Six others (Escherichia coli LysR, IlvY, CysB; Salmonella...
Mikulka, T. W., Stieglitz, B. I., Calvo, J. M.
Leucyl-transfer ribonucleic acid (tRNA) synthetase was purified 100-fold from extracts of Salmonella typhimurium. The partially purified enzyme had the following Km values: leucine, 1.1 × 10−5m;...
Wang, Q, Albert, F G, Fitzgerald, D J, Calvo, J M, Anderson, J N
Previous studies have shown that the promoter/regulatory region of the ilvlH operon displays intrinsic curvature, with the bend center located at position -120 relative to the transcription start...
The nucleotide sequence of a rat myosin light chain 2 gene.
Nudel, U, Calvo, J M, Shani, M, Levy, Z
A rat myosin light chain 2 gene was characterized by nucleotide sequence and S1 mapping analyses. It contains seven exons separated by six introns. The corresponding mRNA is predicted to be 654...
Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
Plasmid pLC1437a contains DNA from Escherichia coli K12 including fol, the structural gene for dihydrofolate reductase. The fol gene was mapped on this plasmid relative to several restriction...
Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12.
Squires, C H, De Felice, M, Devereux, J, Calvo, J M
ilvIH of Escherichia coli K12 codes for a valine-sensitive acetohydroxy acid synthase (AHASIII). The DNA sequence of ilvIH was determined. Open reading frames and appropriate translation signals...
The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli.
The leucine-responsive regulatory protein (Lrp) regulates the expression of more than 40 genes and proteins in Escherichia coli. Among the operons that are positively regulated by Lrp are operons...
Carter, P W, Bartkus, J M, Calvo, J M
The leucine operon of Salmonella typhimurium is controlled by a transcription attenuation mechanism. Four adjacent leucine codons within a 160-nucleotide leu leader RNA are thought to play a central...
Transcriptional proofreading in Escherichia coli.
Libby, R T, Nelson, J L, Calvo, J M, Gallant, J A
A novel transcriptional proofreading mechanism associated with the beta-subunit of wild-type RNA polymerase from Escherichia coli is suggested from the following data. The purified holoenzyme...
The trp, phe, his, thr, and leu operons of enteric bacteria are regulated by a transcriptional attenuation mechanism. Under conditions of amino acid sufficiency, transcription terminates at an...
leu operon of Salmonella typhimurium is controlled by an attenuation mechanism.
Gemmill, R M, Wessler, S R, Keller, E B, Calvo, J M
The nucleotide sequence of the control region of the leu operon of Salmonella typhimurium was determined. A prominent feature of this region is a signal for termination of transcription. In vitro,...
Lrp (Leucine-responsive regulatory protein) is a global regulatory protein that controls the expression of many operons in Escherichia coli. One of those operons, ilvIH, contains six Lrp binding...
Braaten, B A, Platko, J V, Van Der Woude, M W, Simons, B H, De Graaf, F K, Calvo, J M, ...
The methylation blocking factor gene (mbf) in Escherichia coli is required for specific methylation inhibition of two DNA GATC sites upstream of the papBA pilin promoter and transcriptional...
The amino acid sequence of Lrp is highly conserved in four enteric microorganisms.
Friedberg, D, Platko, J V, Tyler, B, Calvo, J M
Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other...
A consensus sequence for binding of Lrp to DNA.
Cui, Y, Wang, Q, Stormo, G D, Calvo, J M
Lrp (leucine-responsive regulatory protein) is a major regulatory protein involved in the expression of numerous operons in Escherichia coli. For ilvIH, one of the operons positively regulated by...
Effects of nutrition and growth rate on Lrp levels in Escherichia coli.
Landgraf, J R, Wu, J, Calvo, J M
Lrp (leucine-responsive regulatory protein) activates some Escherichia coli operons that function in anabolism and represses others involved in catabolism (for a review, see J. M. Calvo and R. G....
Lrp is a regulatory protein in Escherichia coli that increases expression of some operons and decreases expression of others. Mutations in Lrp were isolated on the basis of their effects on ilvIH,...
Regulation of the Escherichia coli lrp gene.
Wang, Q, Wu, J, Friedberg, D, Plakto, J, Calvo, J M
Lrp (leucine-responsive regulatory protein) is a major Escherichia coli regulatory protein which regulates expression of a number of operons, some negatively and some positively. This work relates to...
Haney, S A, Platko, J V, Oxender, D L, Calvo, J M
We investigated the relationship between two regulatory genes, livR and lrp, that map near min 20 on the Escherichia coli chromosome. livR was identified earlier as a regulatory gene affecting...
Transcriptional regulation of the ilv-leu operon of Bacillus subtilis.
Grandoni, J A, Zahler, S A, Calvo, J M
We used primer extension and mutational analysis to identify a promoter upstream of ilvB, the first gene in the ilv-leu operon of Bacillus subtilis. Between the promoter and ilvB, there is a 482-bp...
Regions of the Bacillus subtilis ilv-leu operon involved in regulation by leucine.
Grandoni, J A, Fulmer, S B, Brizzio, V, Zahler, S A, Calvo, J M
The ilv-leu operon of Bacillus subtilis is regulated in part by transcription attenuation. The cis-acting elements required for regulation by leucine lie within a 683-bp fragment of DNA from the...
Bartkus, J M, Tyler, B, Calvo, J M
Four adjacent Leu codons within the leu leader RNA are critically important in transcription attenuation-mediated control of leu operon expression in Salmonella typhimurium and Escherichia coli (P....
In vitro transcription from the Escherichia coli ilvIH promoter.
Lrp (leucine-responsive regulatory protein) activates the expression of the Escherichia coli ilvIH operon in vivo and mediates the repression of the operon by exogenous leucine. In previous studies,...
A protein that binds to the regulatory region of the Escherichia coli ilvIH operon.
Ricca, E, Aker, D A, Calvo, J M
The ilvIH operon of Escherichia coli encodes acetohydroxyacid synthase III, an isoenzyme involved in branched-chain amino acid biosynthesis. Transcription of the ilvIH operon is repressed by growing...
The ilvIH operon of Escherichia coli is positively regulated.
Platko, J V, Willins, D A, Calvo, J M
The ilvIH operon of Escherichia coli (located near min 2) encodes acetohydroxyacid synthase III, an isozyme involved in branched-chain amino acid biosynthesis. A strain with lacZ fused to the ilvIH...
Escherichia coli B/r leuK mutant lacking pseudouridine synthase I activity.
Searles, L L, Jones, J W, Fournier, M J, Grambow, N, Tyler, B, Calvo, J M
Escherichia coli B/r strain EB146 containing mutation leuK16 has elevated levels of enzymes involved in the synthesis of leucine, valine, isoleucine, histidine, and tryptophan (Brown et al., J....
Haughn, G W, Wessler, S R, Gemmill, R M, Calvo, J M
The nucleotide sequence of over 800 base pairs of DNA upstream of leuP was determined for Escherichia coli and Salmonella typhimurium. In both of these enteric bacteria, approximately 500 base pairs...
Promoter mutation causing catabolite repression of the Salmonella typhimurium leucine operon.
Gemmill, R M, Tripp, M, Friedman, S B, Calvo, J M
Two mutations that affect expression of the Salmonella typhimurium leu operon were investigated. leu operon DNA from these mutant strains was cloned, and nucleotide sequences of the leu control...
Carter, P W, Weiss, D L, Weith, H L, Calvo, J M
In Salmonella typhimurium, expression of the leucine operon is regulated by a transcription attenuation mechanism. According to a current model of attenuation, elevated expression of this operon...
Physical characterization of the ilvHI operon of Escherichia coli K-12.
Squires, C H, De Felice, M, Wessler, S R, Calvo, J M
The ilvHI and leu genes of Escherichia coli K-12 are contained on a single 10.9-kilobase EcoRI fragment of deoxyribonucleic acid derived from the leu transducing phage lambda G4. Since the expression...
IlvHI locus of Salmonella typhimurium.
Squires, C H, De Felice, M, Lago, C T, Calvo, J M
In Escherichia coli K-12, the ilvHI locus codes for one of two acetohydroxy acid synthase isoenzymes. A region of the Salmonella typhimurium genome adjacent to the leucine operon was cloned on...
Unusual organization of the ilvIH promoter of Escherichia coli.
Haughn, G W, Squires, C H, De Felice, M, Largo, C T, Calvo, J M
Analysis of plasmids containing ilvIH-galK fusions indicated that the Escherichia coli ilvIH promoter and sequences sufficient to cause leucine repression lie within 363 base pairs (bp) of ilvI....
Isolation and characterization of lambda pleu bacteriophages.
In the Escherichia coli lysogen HfrH73 described by Shimada et al. (1973), none of the enzymes coded for by the leucine operon is synthesized due to an insertion of phage lambda into cistron leuA....
Distribution of the Isopropylmalate Pathway to Leucine Among Diverse Bacteria
Stieglitz, B. I., Calvo, J. M.
α-Isopropylmalate synthase and β-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: Chromatium D, Rhodopseudomonas spheroides, Hydrogenomonas H16,...
Effect of 4-Azaleucine upon Leucine Metabolism in Salmonella typhimurium1
dl-4-Azaleucine (5 × 3−3m) added to exponentially growing cells of Salmonella typhimurium resulted in an abrupt cessation of growth lasting 4 to 8 hr followed by a resumption of division. The...
Recognition of an Escherichia Operator by a Salmonella Repressor
Brandriss, Marjorie C., Calvo, J. M.
Escherichia coli leu+ episomes were transferred to Salmonella typhimurium. The levels of two leucine-forming enzymes were normal (low) in the resulting merodiploids, suggesting that the putative...
Mutants of Salmonella typhimurium with an Altered Leucyl-Transfer Ribonucleic Acid Synthetase
Alexander, Renee R., Calvo, J. M., Freundlich, M.
Two trifluoroleucine-resistant mutants of Salmonella typhimurium, strains CV69 and CV117, had an altered leucyl-transfer ribonucleic acid (tRNA) synthetase. The mutant enzymes had higher apparent Km...
Salmonella Locus Affecting Phosphoenolpyruvate Synthase Activity Identified by a Deletion Analysis
Calvo, J. M., Goodman, M., Salgo, M., Capes, N.
Strain leu-4017, derived from Salmonella typhimurium LT2, cannot utilize acetate, pyruvate, or citric acid cycle intermediates as sole sources of carbon. The mutation in this strain extends from the...
Calvo, J. M., Freundlich, M., Umbarger, H. E.
5′,5′,5′-Trifluoro-dl-leucine inhibited the activity of α-isopropylmalate synthetase (the initial enzyme unique to leucine biosynthesis) as well as the growth of Salmonella typhimurium....
A large family of bacterial activator proteins.
Henikoff, S, Haughn, G W, Calvo, J M, Wallace, J C
At least nine different bacterial proteins belong to the LysR family. The gene sequence for one of these proteins is presented here. Six others (Escherichia coli LysR, IlvY, CysB; Salmonella...
Mikulka, T. W., Stieglitz, B. I., Calvo, J. M.
Leucyl-transfer ribonucleic acid (tRNA) synthetase was purified 100-fold from extracts of Salmonella typhimurium. The partially purified enzyme had the following Km values: leucine, 1.1 × 10−5m;...
Wang, Q, Albert, F G, Fitzgerald, D J, Calvo, J M, Anderson, J N
Previous studies have shown that the promoter/regulatory region of the ilvlH operon displays intrinsic curvature, with the bend center located at position -120 relative to the transcription start...
The nucleotide sequence of a rat myosin light chain 2 gene.
Nudel, U, Calvo, J M, Shani, M, Levy, Z
A rat myosin light chain 2 gene was characterized by nucleotide sequence and S1 mapping analyses. It contains seven exons separated by six introns. The corresponding mRNA is predicted to be 654...
Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
Plasmid pLC1437a contains DNA from Escherichia coli K12 including fol, the structural gene for dihydrofolate reductase. The fol gene was mapped on this plasmid relative to several restriction...
Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12.
Squires, C H, De Felice, M, Devereux, J, Calvo, J M
ilvIH of Escherichia coli K12 codes for a valine-sensitive acetohydroxy acid synthase (AHASIII). The DNA sequence of ilvIH was determined. Open reading frames and appropriate translation signals...
The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli.
The leucine-responsive regulatory protein (Lrp) regulates the expression of more than 40 genes and proteins in Escherichia coli. Among the operons that are positively regulated by Lrp are operons...
Carter, P W, Bartkus, J M, Calvo, J M
The leucine operon of Salmonella typhimurium is controlled by a transcription attenuation mechanism. Four adjacent leucine codons within a 160-nucleotide leu leader RNA are thought to play a central...
Transcriptional proofreading in Escherichia coli.
Libby, R T, Nelson, J L, Calvo, J M, Gallant, J A
A novel transcriptional proofreading mechanism associated with the beta-subunit of wild-type RNA polymerase from Escherichia coli is suggested from the following data. The purified holoenzyme...
The trp, phe, his, thr, and leu operons of enteric bacteria are regulated by a transcriptional attenuation mechanism. Under conditions of amino acid sufficiency, transcription terminates at an...
leu operon of Salmonella typhimurium is controlled by an attenuation mechanism.
Gemmill, R M, Wessler, S R, Keller, E B, Calvo, J M
The nucleotide sequence of the control region of the leu operon of Salmonella typhimurium was determined. A prominent feature of this region is a signal for termination of transcription. In vitro,...
Lrp (Leucine-responsive regulatory protein) is a global regulatory protein that controls the expression of many operons in Escherichia coli. One of those operons, ilvIH, contains six Lrp binding...