Mutational properties of the primary aflatoxin B1-DNA adduct.
Bailey, E A, Iyer, R S, Stone, M P, Harris, T M, Essigmann, J M
The mutagenic activity of the major DNA adduct formed by the liver carcinogen aflatoxin B1 (AFB1) was investigated in vivo. An oligonucleotide containing a single...
X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine.
Lipscomb, L A, Peek, M E, Morningstar, M L, Verghis, S M, Miller, E M, Rich, A, ...
We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The...
Treiber, D K, Zhai, X, Jantzen, H M, Essigmann, J M
The toxicity of DNA-damaging agents is widely believed to result from the formation of lesions that block polymerases or disrupt the integrity of the genome. A mechanism heretofore not addressed is...
Bruhn, S L, Pil, P M, Essigmann, J M, Housman, D E, Lippard, S J
Human cDNA clones encoding a structure-specific recognition protein, SSRP1, that binds specifically to DNA modified with cisplatin have been isolated and characterized. The SSRP1 gene maps to human...
Szymkowski, D E, Yarema, K, Essigmann, J M, Lippard, S J, Wood, R D
We have examined the ability of human cell extracts to repair the most frequent DNA adduct caused by the cancer chemotherapeutic agent cis-diamminedichloroplatinum(II). A circular DNA duplex with an...
A viral genome containing an unstable aflatoxin B1-N7-guanine DNA adduct situated at a unique site.
Bailey, E A, Iyer, R S, Harris, T M, Essigmann, J M
A problem that has hindered the study of the biological properties of certain DNA adducts, such as those that form at the N7 atoms of purines, is their extreme chemical lability. Conditions are...
Substrate specificity of ultraviolet DNA endonuclease (UVDE/Uve1p) from Schizosaccharomyces pombe.
Avery, A M, Kaur, B, Taylor, J S, Mello, J A, Essigmann, J M, Doetsch, P W
Schizosaccharomyces pombe ultraviolet DNA endonuclease (UVDE or Uve1p) has been shown to cleave 5' to UV light-induced cyclobutane pyrimidine dimers (CPDs) and pyrimidine-pyrimidone (6-4)...
Separation of the SOS-dependent and SOS-independent components of alkylating-agent mutagenesis.
Couto, L B, Chaudhuri, I, Donahue, B A, Demple, B, Essigmann, J M
Escherichia coli plasmids containing the rpsL+ gene (Strs phenotype) as the target for mutation were treated in vitro with N-methyl-N-nitrosourea. Following fixation of mutations in E. coli MM294A...
Characterization of a mammalian homolog of the Escherichia coli MutY mismatch repair protein.
McGoldrick, J P, Yeh, Y C, Solomon, M, Essigmann, J M, Lu, A L
A protein homologous to the Escherichia coli MutY protein, referred to as MYH, has been identified in nuclear extracts of calf thymus and human HeLa cells. Western blot (immunoblot) analysis using...
Genetic effects of thymine glycol: site-specific mutagenesis and molecular modeling studies.
Basu, A K, Loechler, E L, Leadon, S A, Essigmann, J M
The mutational specificity and genetic requirements for mutagenesis by 5,6-dihydroxy-5,6-dihydrothymine (thymine glycol), one of the principal DNA lesions induced by oxidation and ionizing radiation,...
Singer, B, Chavez, F, Goodman, M F, Essigmann, J M, Dosanjh, M K
O6-Methylguanine (m6G) was incorporated site-specifically into two 25-base oligonucleotides differing only in the nucleotide on the 3' side of the modified base. Templates were primed with...
Toney, J H, Donahue, B A, Kellett, P J, Bruhn, S L, Essigmann, J M, Lippard, S J
DNA modified by the antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP or cisplatin) was used to identify a factor in mammalian cells that binds to cis-DDP-damaged DNA and hence may play a role...
Ellison, K S, Dogliotti, E, Connors, T D, Basu, A K, Essigmann, J M
A plasmid was constructed in which a single guanine residue was replaced with either O6-methylguanine or O6-ethylguanine, two of the DNA adducts formed by carcinogenic alkylating agents. The vectors...
Yarema, K J, Lippard, S J, Essigmann, J M
The toxicity and mutagenicity of three DNA adducts formed by the anticancer drug cis-diamminedichloroplatinum(II) (cis-DDP or cisplatin) were investigated in Escherichia coli. The adducts studied...
Treiber, D K, Chen, Z, Essigmann, J M
The binding specificity was defined of a human ultraviolet light-damaged DNA recognition protein (UV-DRP), the activity of which is absent in some xeroderma pigmentosum complementation group E cells....
Wood, M L, Esteve, A, Morningstar, M L, Kuziemko, G M, Essigmann, J M
A single 7,8-dihydro-8-oxoguanine (G8-OXO; 8-hydroxyguanine) adduct in the lacZ alpha gene of bacteriophage M13 DNA induces a targeted G-->T transversion after replication in Escherichia coli...
Green, C L, Loechler, E L, Fowler, K W, Essigmann, J M
Organic synthesis and recombinant DNA technology were used to situate a putatively premutagenic DNA lesion, O6-methylguanine (O6MeGua), at a specific location in the genomes of two bacterial viruses,...
Essigmann, J M, Barker, L J, Fowler, K W, Francisco, M A, Reinhold, V N, Wogan, G N
Sterigmatocystin (ST), a potent hepatocarcinogen, was covalently bound to calf thymus DNA by incubation in the presence of phenobarbital-induced rat liver microsomes. Acid hydrolysis of ST-modified...
Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium.
Stark, A A, Essigmann, J M, Demain, A L, Skopek, T R, Wogan, G N
Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to...
In vivo mutagenesis by O6-methylguanine built into a unique site in a viral genome.
Loechler, E L, Green, C L, Essigmann, J M
The mutagenicity of O6-methylguanine (O6MeGua), a chemical carcinogen-DNA adduct, has been studied in vivo by using a single-stranded M13mp8 genome in which a single O6MeGua residue was positioned in...
Identification of the principal aflatoxin B1-DNA adduct formed in vivo in rat liver.
Croy, R G, Essigmann, J M, Reinhold, V N, Wogan, G N
The products of in vivo covalent binding of activated aflatoxin B1 (AFB1) to DNA have been investigated in rats. The principal covalent product formed in liver DNA of rats treated with AFB1 has been...
Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro.
Essigmann, J M, Croy, R G, Nadzan, A M, Busby, W F, Reinhold, V N, Büchi, G, ...
The covalent binding of the hepatocarcinogen aflatoxin B1 by rat liver microsomes to calf thymus DNA resulted in a binding level equal to one aflatoxin residue per 60 DNA nucleotides. An aflatoxin...
Essigmann, J M, Fowler, K W, Green, C L, Loechler, E L
This work examines the mutagenic activity of O6-methylguanine (O6MeGua), a DNA adduct formed by certain carcinogenic alkylating agents. A tetranucleotide, 5'-HOTpm6GpCpA-3', was synthesized and...
Mutational properties of the primary aflatoxin B1-DNA adduct.
Bailey, E A, Iyer, R S, Stone, M P, Harris, T M, Essigmann, J M
The mutagenic activity of the major DNA adduct formed by the liver carcinogen aflatoxin B1 (AFB1) was investigated in vivo. An oligonucleotide containing a single...
X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine.
Lipscomb, L A, Peek, M E, Morningstar, M L, Verghis, S M, Miller, E M, Rich, A, ...
We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The...
Treiber, D K, Zhai, X, Jantzen, H M, Essigmann, J M
The toxicity of DNA-damaging agents is widely believed to result from the formation of lesions that block polymerases or disrupt the integrity of the genome. A mechanism heretofore not addressed is...
Bruhn, S L, Pil, P M, Essigmann, J M, Housman, D E, Lippard, S J
Human cDNA clones encoding a structure-specific recognition protein, SSRP1, that binds specifically to DNA modified with cisplatin have been isolated and characterized. The SSRP1 gene maps to human...
Szymkowski, D E, Yarema, K, Essigmann, J M, Lippard, S J, Wood, R D
We have examined the ability of human cell extracts to repair the most frequent DNA adduct caused by the cancer chemotherapeutic agent cis-diamminedichloroplatinum(II). A circular DNA duplex with an...
A viral genome containing an unstable aflatoxin B1-N7-guanine DNA adduct situated at a unique site.
Bailey, E A, Iyer, R S, Harris, T M, Essigmann, J M
A problem that has hindered the study of the biological properties of certain DNA adducts, such as those that form at the N7 atoms of purines, is their extreme chemical lability. Conditions are...
Substrate specificity of ultraviolet DNA endonuclease (UVDE/Uve1p) from Schizosaccharomyces pombe.
Avery, A M, Kaur, B, Taylor, J S, Mello, J A, Essigmann, J M, Doetsch, P W
Schizosaccharomyces pombe ultraviolet DNA endonuclease (UVDE or Uve1p) has been shown to cleave 5' to UV light-induced cyclobutane pyrimidine dimers (CPDs) and pyrimidine-pyrimidone (6-4)...
Separation of the SOS-dependent and SOS-independent components of alkylating-agent mutagenesis.
Couto, L B, Chaudhuri, I, Donahue, B A, Demple, B, Essigmann, J M
Escherichia coli plasmids containing the rpsL+ gene (Strs phenotype) as the target for mutation were treated in vitro with N-methyl-N-nitrosourea. Following fixation of mutations in E. coli MM294A...
Characterization of a mammalian homolog of the Escherichia coli MutY mismatch repair protein.
McGoldrick, J P, Yeh, Y C, Solomon, M, Essigmann, J M, Lu, A L
A protein homologous to the Escherichia coli MutY protein, referred to as MYH, has been identified in nuclear extracts of calf thymus and human HeLa cells. Western blot (immunoblot) analysis using...
Genetic effects of thymine glycol: site-specific mutagenesis and molecular modeling studies.
Basu, A K, Loechler, E L, Leadon, S A, Essigmann, J M
The mutational specificity and genetic requirements for mutagenesis by 5,6-dihydroxy-5,6-dihydrothymine (thymine glycol), one of the principal DNA lesions induced by oxidation and ionizing radiation,...
Singer, B, Chavez, F, Goodman, M F, Essigmann, J M, Dosanjh, M K
O6-Methylguanine (m6G) was incorporated site-specifically into two 25-base oligonucleotides differing only in the nucleotide on the 3' side of the modified base. Templates were primed with...
Toney, J H, Donahue, B A, Kellett, P J, Bruhn, S L, Essigmann, J M, Lippard, S J
DNA modified by the antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP or cisplatin) was used to identify a factor in mammalian cells that binds to cis-DDP-damaged DNA and hence may play a role...
Ellison, K S, Dogliotti, E, Connors, T D, Basu, A K, Essigmann, J M
A plasmid was constructed in which a single guanine residue was replaced with either O6-methylguanine or O6-ethylguanine, two of the DNA adducts formed by carcinogenic alkylating agents. The vectors...
Yarema, K J, Lippard, S J, Essigmann, J M
The toxicity and mutagenicity of three DNA adducts formed by the anticancer drug cis-diamminedichloroplatinum(II) (cis-DDP or cisplatin) were investigated in Escherichia coli. The adducts studied...
Treiber, D K, Chen, Z, Essigmann, J M
The binding specificity was defined of a human ultraviolet light-damaged DNA recognition protein (UV-DRP), the activity of which is absent in some xeroderma pigmentosum complementation group E cells....
Wood, M L, Esteve, A, Morningstar, M L, Kuziemko, G M, Essigmann, J M
A single 7,8-dihydro-8-oxoguanine (G8-OXO; 8-hydroxyguanine) adduct in the lacZ alpha gene of bacteriophage M13 DNA induces a targeted G-->T transversion after replication in Escherichia coli...
Green, C L, Loechler, E L, Fowler, K W, Essigmann, J M
Organic synthesis and recombinant DNA technology were used to situate a putatively premutagenic DNA lesion, O6-methylguanine (O6MeGua), at a specific location in the genomes of two bacterial viruses,...
Essigmann, J M, Barker, L J, Fowler, K W, Francisco, M A, Reinhold, V N, Wogan, G N
Sterigmatocystin (ST), a potent hepatocarcinogen, was covalently bound to calf thymus DNA by incubation in the presence of phenobarbital-induced rat liver microsomes. Acid hydrolysis of ST-modified...
Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium.
Stark, A A, Essigmann, J M, Demain, A L, Skopek, T R, Wogan, G N
Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to...
In vivo mutagenesis by O6-methylguanine built into a unique site in a viral genome.
Loechler, E L, Green, C L, Essigmann, J M
The mutagenicity of O6-methylguanine (O6MeGua), a chemical carcinogen-DNA adduct, has been studied in vivo by using a single-stranded M13mp8 genome in which a single O6MeGua residue was positioned in...
Identification of the principal aflatoxin B1-DNA adduct formed in vivo in rat liver.
Croy, R G, Essigmann, J M, Reinhold, V N, Wogan, G N
The products of in vivo covalent binding of activated aflatoxin B1 (AFB1) to DNA have been investigated in rats. The principal covalent product formed in liver DNA of rats treated with AFB1 has been...
Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro.
Essigmann, J M, Croy, R G, Nadzan, A M, Busby, W F, Reinhold, V N, Büchi, G, ...
The covalent binding of the hepatocarcinogen aflatoxin B1 by rat liver microsomes to calf thymus DNA resulted in a binding level equal to one aflatoxin residue per 60 DNA nucleotides. An aflatoxin...
Essigmann, J M, Fowler, K W, Green, C L, Loechler, E L
This work examines the mutagenic activity of O6-methylguanine (O6MeGua), a DNA adduct formed by certain carcinogenic alkylating agents. A tetranucleotide, 5'-HOTpm6GpCpA-3', was synthesized and...