Dorenbos, R., Stein, T., Kabel, J., Bruand, C., Bolhuis, A., Bron, S., ...
Tjalsma, H, Van Dijl, J M, Bron, S, ...
Type I signal peptidases (SPases) are a widespread family of enzymes which remove signal peptides from proteins translocated across cellular membranes. Here, we report the first isolation of a gene...
Van Schie, B.J., Hellingwerf, K.J., Van Dijken, J.P., Elferink, M.G.L., Van Dijl, J.M., Kuenen, J.G., ...
The coupling of membrane-bound glucose dehydrogenase (EC 1.1.99.17) to the respiratory chain was studied in whole cells, cell-free exts., and membrane vesicles of gram-neg. bacteria. Several E. coli...
Van Roosmalen, M. L., Jongbloed, J. D. H., Kuipers, A., Venema, G., Bron, S., Van Dijl, J. M.
Soluble forms of Bacillus signal peptidases which lack their unique amino-terminal membrane anchor are prone to degradation, which precludes their high-level production in the cytoplasm of...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Cordes, C, Meima, R, Twiest, B, Kazemier, B, Venema, G, Van Dijl, J M, ...
The rolling-circle plasmid pGP1 was used to study the effects of the expression of a plasmid-specified exported protein on structural plasmid stability in Bacillus subtilis. pGP1 contains a fusion...
Van Schie, B J, Hellingwerf, K J, Van Dijken, J P, Elferink, M G, Van Dijl, J M, Kuenen, J G, ...
The coupling of membrane-bound glucose dehydrogenase (EC 1.1.99.17) to the respiratory chain has been studied in whole cells, cell-free extracts, and membrane vesicles of gram-negative bacteria....
Kok, J, Van Dijl, J M, Van Der Vossen, J M, Venema, G
Previously, curing experiments suggested that plasmid pWV05 (17.5 megadaltons [Md]) of Streptococcus cremoris Wg2 specifies proteolytic activity. A restriction enzyme map of pWV05 was constructed,...
Van Dijl, J M, De Jong, A, Vehmaanperä, J, Venema, G, Bron, S
Signal peptidases (SPases) remove signal peptides from secretory proteins. The sipS (signal peptidase of subtilis) gene, which encodes an SPase of Bacillus subtilis, was cloned in Escherichia coli...
Van Roosmalen, M. L., Jongbloed, J. D. H., Kuipers, A., Venema, G., Bron, S., Van Dijl, J. M.
Soluble forms of Bacillus signal peptidases which lack their unique amino-terminal membrane anchor are prone to degradation, which precludes their high-level production in the cytoplasm of...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Cordes, C, Meima, R, Twiest, B, Kazemier, B, Venema, G, Van Dijl, J M, ...
The rolling-circle plasmid pGP1 was used to study the effects of the expression of a plasmid-specified exported protein on structural plasmid stability in Bacillus subtilis. pGP1 contains a fusion...
Van Schie, B J, Hellingwerf, K J, Van Dijken, J P, Elferink, M G, Van Dijl, J M, Kuenen, J G, ...
The coupling of membrane-bound glucose dehydrogenase (EC 1.1.99.17) to the respiratory chain has been studied in whole cells, cell-free extracts, and membrane vesicles of gram-negative bacteria....
Kok, J, Van Dijl, J M, Van Der Vossen, J M, Venema, G
Previously, curing experiments suggested that plasmid pWV05 (17.5 megadaltons [Md]) of Streptococcus cremoris Wg2 specifies proteolytic activity. A restriction enzyme map of pWV05 was constructed,...
Van Dijl, J M, De Jong, A, Vehmaanperä, J, Venema, G, Bron, S
Signal peptidases (SPases) remove signal peptides from secretory proteins. The sipS (signal peptidase of subtilis) gene, which encodes an SPase of Bacillus subtilis, was cloned in Escherichia coli...
Mapping the Pathways to Staphylococcal Pathogenesis by Comparative Secretomics
Ziebandt, A. K., Engelmann, S., Hecker, M., De Jong, A., Harmsen, H. J. M., ...
The gram-positive bacterium Staphylococcus aureus is a frequent component of the human microbial flora that can turn into a dangerous pathogen. As such, this organism is capable of infecting almost...
The chemistry and enzymology of the type I signal peptidases.
Dalbey, R. E., Lively, M. O., Bron, S., Van Dijl, J. M.
The discovery that proteins exported from the cytoplasm are typically synthesized as larger precursors with cleavable signal peptides has focused interest on the peptidases that remove the signal...