The genus Nucleopolyhedrovirus (NPV) of the family Baculoviridae can be subdivided phylogenetically into two groups. The same division can be made on the basis of their budded virus (BV) envelope...
The heptad repeat (HR), a conserved structural motif of class I viral fusion proteins, is responsible for the formation of a six-helix bundle structure during the envelope fusion process. The insect...
Zwart, M.P., Cory, J.S., Vlak, J.M.
Two bacmid-derived Autographa californica Multiple-capsid Nucleopolyhedrovirus genotypes ¿ that differ only in a short tag sequence for differential PCR recognition ¿ were generated. By electron...
The F protein is the major glycoprotein present in the envelopes of budded virus (BV) of members of the family Baculoviridae. The F protein mediates low-pH-activated fusion with insect cell...
Wang, Y., Crawford, A.M., Vlak, J.M., Jehle, J.A.
Oryctes rhinoceros virus (OrV) is an unassigned invertebrate dsDNA virus with enveloped and rod-shaped virions. Two cloned PstI fragments, C and D, of OrV DNA have been sequenced, consisting of...
Huang, Yi, Deng, F., Hu, Z.H., Vlak, J.M., Wang, H.
Double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful reverse genetics tool to silence gene expression in multiple organisms, including plants, nematodes and insects. In...
The Chilo iridescent virus DNA polymerase promoter contains an essential AAAAT motif (2007)
Nalcacioglu, R., Ince, I.A., Vlak, J.M., Demirbag, Z
The delayed-early DNA polymerase promoter of Chilo iridescent virus (CIV), officially known as Invertebrate iridescent virus, was fine mapped by constructing a series of increasing deletions and by...
The Chilo iridescent virus DNA polymerase promoter contains an essential AAAAT motif (2007)
Nalcacioglu, R., Ince, I.A., Vlak, J.M., Demirbag, Z
The delayed-early DNA polymerase promoter of Chilo iridescent virus (CIV), officially known as Invertebrate iridescent virus, was fine mapped by constructing a series of increasing deletions and by...
Herrero, S., Ansems, M., Vlak, J.M., Bakker, P.L.
Insect larvae spend most of their time eating and the digestive tract is the most crucial barrier for the entrance of many pathogens. In our study, suppression subtractive hybridization (SSH) was...
Westenberg, M., Uijtdewilligen, P., Vlak, J.M.
Group II nucleopolyhedroviruses (NPVs), e.g. Helicoverpa armigera (Hear) NPV and Spodoptera exigua (Se) MNPV (multiple NPV), lack a GP64-like protein that is present in group I NPVs, e.g. Autographa...
Pan, X., Long, G., Wang, R.R., Hou, S., Wang, H., Zheng, Y., ...
The open reading frame Ha107 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) encodes a putative protein of 51 kDa with homologues in a few group II NPVs and a granulovirus....
Westenberg, M., Uijtdewilligen, P., Vlak, J.M.
Group II nucleopolyhedroviruses (NPVs), e.g. Helicoverpa armigera (Hear) NPV and Spodoptera exigua (Se) MNPV (multiple NPV), lack a GP64-like protein that is present in group I NPVs, e.g. Autographa...
Wang, Y., Kleespies, R.G., Ale, B., Ramle, M.B., Jackson, T., ...
Baculovirus genomes are covalently closed circles of double stranded-DNA varying in size between 80 and 180 kilobase-pair. The genomes of more than fourty-one baculoviruses have been sequenced to...
Jakubowska, A., Peters, S.A., Ziemnicka, J., Vlak, J.M.
The genome sequence of a Polish isolate of Agrotis segetum nucleopolyhedrovirus (AgseNPV-A) was determined and analysed. The circular genome is composed of 147 544 bp and has a G+C content of 45¿7...
Herniou, E.A., Peters, S., Vlak, J.M.
The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an...
Long, G., Westenberg, M., Wang, H, Vlak, J.M., Hu, Z.
In the family Baculoviridae, two distinct envelope fusion proteins are identified in budded virions (BVs). GP64 is the major envelope fusion protein of group I nucleopolyhedrovirus (NPV) BVs. An...
Pijlman, G.P., Roode, E.C., Fan, X.X., Roberts, L.O., Belsham, G.J., Vlak, J.M., ...
The efficient scale-up of recombinant protein production in insect-cell bioreactors using baculovirus expression vectors is hampered by reductions in yield with increasing viral passage, the...
In silico identification of putative promoter motifs of White Spot syndrome virus (2006)
Marks, H., Ren, X.Y., Sandbrink, H., Vlak, J.M.
Background: White Spot Syndrome Virus, a member of the virus family Nimaviridae, is a large dsDNA virus infecting shrimp and other crustacean species. Although limited information is available on the...
It has been generally accepted that invertebrates such as shrimp do not have an adaptive immune response system comparable to that of vertebrates. However, in the last few years, several studies have...
Fang, M, Nie, Y., Wang, Q., Deng, F., Wang, R., Wang, H, ...
Open reading frame 132 (Ha132) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) is a homologue of per os infectivity factor 2 (pif-2) of Spodoptera exigua multiple nucleopolyhedrovirus....
A comprehensive model was developed to simulate virus epizootics in a stage structured insect population and analyse scenarios for the biological control of cotton bollworm (CBW), Helicoverpa...
Long, G., Pan, X., Kormelink, R.J.M., Vlak, J.M.
Entry of the budded virus form of baculoviruses into insect and mammalian cells is generally thought to occur through a low-pH-dependent endocytosis pathway, possibly through clathrin-coated pits....
On the classification and nomenclature of baculoviruses: A proposal for revision (2006)
Jehle, J.A., Blissard, G.W., Bonning, B.C., Cory, J.S., Herniou, E.A., Rohrmann, G.F., ...
Recent evidence from genome sequence analyses demands a substantial revision of the taxonomy and classification of the family Baculoviridae. Comparisons of 29 baculovirus genomes indicated that...
Ongus, J.R., Roode, E.C., Pleij, C.W.A, Vlak, J.M.
Structure prediction of the 5' non-translated region (NTR) of four iflavirus RNAs revealed two types of potential internal ribosome entry site (IRES), which are discriminated by size and level of...
Robalino, J., Payne, C., Parnell, P., Shepard, E., Grimes, A.C., Metz, A., ...
White Spot Syndrome Virus (WSSV) is a highly pathogenic and prevalent virus affecting crustacea. A number of WSSV envelope proteins, including vp28, have been proposed to be involved in viral...
Long, G., Pan, M., Westenberg, M., Vlak, J.M.
F proteins from baculovirus nucleopolyhedrovirus (NPV) group II members are the major budded virus (BV) viral envelope fusion proteins. They undergo furin-like proteolysis processing in order to be...
Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (2006)
Ha 1 35, a unique nonstructural protein of HearNPV, is not essential for viral propagation (2006)
Pan, X., Long, G., Westenberg, M., Hou, S., Deng, F., Wang, H, ...
Herniou, E.A., Peters, S., Vlak, J.M.
The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an...
Sun, X., Sun, X.C., Bai, B, Vlak, J.M., Hu, Z
We report on the yield of Polyhedral Inclusion Bodies (PIBs) from first to fifth instar Helicoverpa armigera larvae inoculated with wild-type Helicoverpa armigera nucleopolyhedrovirus (HaSNPV-WT) or...
Gene-expression profiling of White spot syndrome virus in vivo (2005)
Marks, H., Vorst, O.F.J., Vlak, J.M.
White spot syndrome virus, type species of the genus Whispovirus in the family Nimaviridae, is a large, double-stranded DNA (dsDNA) virus that infects crustaceans. The genome of the completely...
Zhou, M., Sun, X., Sun, X.C., Vlak, J.M., Hu, Z.H.
Transmission plays a central role in the ecology of baculoviruses and the population dynamics of their hosts. Here, we report on the horizontal and vertical transmission dynamics of wild-type...
Identification and recombinant expression of a novel chymotrypsin from Spodoptera exigua (2005)
Herrero, S., Combes, E, Vlak, J.M., Beekwilder, M.J.
A novel chymotrypsin which is expressed in the midgut of the lepidopteran insect Spodoptera exigua is described. This enzyme, referred to as SeCT34, represents a novel class of chymotrypsins. Its...
Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland (2005)
Jakubowska, A., Ziemnicka, J., Lipa, J.J., Vlak, J.M.
The turnip moth, Agrotis segetum (Lepidoptera, Noctuidae), is an important pest insect in Europe, Asia, and Africa. We have genetically characterized and classified a nucleopolyhedrovirus isolated...
Fitness and virulence of an ancestral White Spot Syndrome Virus isolate from shrimp (2005)
Marks, H., Duijse, J.J.A., Zuidema, D., Vlak, J.M.
White Spot Syndrome Virus, the type species of the virus family Nimaviridae, is a large dsDNA virus infecting shrimp and other crustaceans. Genomic analysis of three completely sequenced WSSV...
Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle (2005)
Kaba, S.A., Musoke, A.J., Schaap, D., Schetters, T., Rowlands, J., Vermeulen, A.J., ...
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion...
European Leucoma salicis NPV is closely related to North American Orgyia pseudotsugata MNPV (2005)
Jakubowska, A., Cory, J.S., Ziemnicka, J., Vlak, J.M.
The satin moth Leucoma salicis L. (Lepidoptera, Lymantriidae) is a frequent defoliator of poplar trees (Populus spp.) in Europe and Asia (China, Japan). Around 1920 the insect was introduced into the...
Een nieuw baculovirus van en voor de Turkse mot (2005)
Messelink, G.J., Peters, S., Vlak, J.M.
Samenvatting van de voordracht te houden op 30 november 2005 tijdens de Najaarsvergadering van de KNPV (Koninklijke Nederlandse Plantenziektekundige Vereniging). Onderzoek naar de mogelijke...
Westenberg, M., Heinhuis, B., Zuidema, D., Vlak, J.M.
White spot syndrome virus (WSSV) is a major disease in crustaceans, particularly shrimp, due to the current intensity of aquaculture practices. Novel strategies including vaccination to control this...
Molecular genetics of White Spot Syndrome Virus (2005)
Marks, H., Ren, X., Witteveldt, J., Sandbrink, H., Vlak, J.M.
Witteveldt, J., Vermeesch, A.M.G., Langenhof, M., Vlak, J.M.
White spot syndrome virus (WSSV) is type species of the genus Whispovirus of the new family Nimaviridae. Despite the elucidation of its genomic sequence, very little is known about the virus as only...
Since the 1990s shrimp culture has been hampered by mass mortalities in ponds throughout the world. Penaeid shrimp are affected by many infectious agents, mainly of bacterial and viral origin, and...
Herrero, S., Ansems, M., Vlak, J.M., Bakker, P.L., Moar, W., ...
The response of insects to pathogens involves changes in gene expression, which may help the insect to overcome the effects of pathogens or their toxins. In our current work, Suppression Subtractive...
On the genomic sequence of the Oryctes rhinoceros virus (2005)
Vlak, J.M., Richards, N., Wang, Y., Jackson, T., Jehle, J.A., Glare, T., ...
The beetle Oryctes rhinoceros (Coleoptera: Scarabaeidae)is a serious pest in coconut and oil palms throughout Southeast Asia and the Pacific. Its control through chemical pesticides frequently failed...
Whole genome sequence analysis of a Polish isolate of Agrotis segetum nucleopolyhedrovirus (2005)
Jakubowska, A., Klein Lankhorst, R.M., Ziemnicka, J., Vlak, J.M.
The turnip moth, Agrotis segetum, is an important pest in many crops in Europe, Asia and Africa. Both nucleopolyhedrovirus (NPV) and granulovirus (GV) species were isolated from this insect with...
Jakubowska, A., Vlak, J.M., Ziemnicka, J.
The fp25k gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was studied. HearNPV fp25k gene transcription was found starting from about 18 h post-infection, and protein...
Jakubowska, A., Vlak, J.M., Ziemnicka, J.
A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR, was characterized...