Mitofusins and OPA1 Mediate Sequential Steps in Mitochondrial Membrane Fusion (2009)
Song, Zhiyin, Ghochani, Mariam, McCaffery, J. Michael, Frey, Terrence G., Chan, David C.
Mitochondrial fusion requires the coordinated fusion of the outer and inner membranes. Three large GTPases—OPA1 and the mitofusins Mfn1 and Mfn2—are essential for the fusion of mammalian...
Bc|-xL increases mitochondrial fission, fusion, and biomass in neurons (2009)
Berman, Sarah B., Chen, Ying-bei, Qi, Bing, McCaffery, J. Michael, Rucker, Edmund B., Goebbels, Sandra, ...
Yiider Tseng, Elena Fedorov, J. Michael Mccaffery, Steven C. Almo, Denis Wirtz
related protein; MSD, mean squared displacement; EM, electron microscopy. E-mail address of the corresponding author:
Reinke, Aaron, Anderson, Scott, McCaffery, J Michael, Yates, John, Aronova, Sofia, Chu, Stephanie, ...
The Tor1p and Tor2p kinases, targets of the therapeutically important antibiotic rapamycin, function as components of two distinct protein complexes in yeast, termed TOR complex 1 (TORC1) and TORC2....
Mitochondrial fission proteins regulate programmed cell death in yeast (2004)
Fannjiang, Yihru, Cheng, Wen-Chih, Lee, Sarah J., Qi, Bing, Pevsner, Jonathan, McCaffery, J. Michael, ...
The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast...
Mitochondrial fission proteins regulate programmed cell death in yeast (2004)
Fannjiang, Yihru, Cheng, Wen-Chih, Lee, Sarah J., Qi, Bing, Pevsner, Jonathan, McCaffery, J. Michael, ...
The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast...
Mitochondrial fission proteins regulate programmed cell death in yeast (2004)
Fannjiang, Yihru, Cheng, Wen-Chih, Lee, Sarah J., Qi, Bing, Pevsner, Jonathan, McCaffery, J. Michael, ...
The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast...
Crooks, Denise, Kil, Song Jae, McCaffery, J. Michael, Carlin, Cathleen, Bonifacino, Juan
Animal cell viruses provide valuable model systems for studying many normal cellular processes, including membrane protein sorting. The focus of this study is an integral membrane protein encoded by...
Crooks, Denise, Kil, Song Jae, McCaffery, J. Michael, Carlin, Cathleen
Animal cell viruses provide valuable model systems for studying many normal cellular processes, including membrane protein sorting. The focus of this study is an integral membrane protein encoded by...
Clathrin-coated vesicles bearing GAIP possess GTPase-activating protein activity in vitro
Fischer, Thierry, Elenko, Eric, McCaffery, J. Michael, DeVries, Luc, Farquhar, Marilyn Gist
Gα-interacting protein (GAIP) is a member of the RGS (regulators of G protein signaling) family, which serve as GAPs (GTPase-activating proteins) for Gα subunits. Previously, we demonstrated that...
Llopis, Juan, McCaffery, J. Michael, Miyawaki, Atsushi, Farquhar, Marilyn G., Tsien, Roger Y.
Many cellular events depend on a tightly compartmentalized distribution of H+ ions across membrane-bound organelles. However, measurements of organelle pH in living cells have been scarce. Several...
De Vries, Luc, Elenko, Eric, McCaffery, J. Michael, Fischer, Thierry, Hubler, Laura, McQuistan, Tammie, ...
RGS-GAIP (Gα-interacting protein) is a member of the RGS (regulator of G protein signaling) family of proteins that functions to down-regulate Gαi/Gαq-linked signaling. GAIP is a GAP or guanosine...
Division of Mitochondria Requires a Novel DNM1-interacting Protein, Net2p
Cerveny, Kara L., McCaffery, J. Michael, Jensen, Robert E.
Mitochondria are dynamic organelles that undergo frequent division and fusion, but the molecular mechanisms of these two events are not well understood. Dnm1p, a mitochondria-associated,...
Collins, Cynthia S., Kalish, Jennifer E., Morrell, James C., McCaffery, J. Michael, Gould, Stephen J.
Peroxisomes are independent organelles found in virtually all eukaryotic cells. Genetic studies have identified more than 20 PEX genes that are required for peroxisome biogenesis. The role of most...
Localization of the Rsp5p Ubiquitin-Protein Ligase at Multiple Sites within the Endocytic Pathway
Wang, Guangli, McCaffery, J. Michael, Wendland, Beverly, Dupré, Sophie, Haguenauer-Tsapis, Rosine, Huibregtse, Jon M.
The Saccharomyces cerevisiae RSP5 gene encodes an essential HECT E3 ubiquitin-protein ligase. Rsp5p contains an N-terminal C2 domain, three WW domains in the central portion of the molecule, and a...
Oh, SangKon, McCaffery, J. Michael, Eichelberger, Maryna C.
During the acute phase of infection with influenza A virus, the degree of lymphopenia correlates with severity of disease. Factors that contribute to T-cell activation during influenza virus...
Desai, Prashant, Sexton, Gerry L., McCaffery, J. Michael, Person, Stanley
The tegument is an integral and essential structural component of the herpes simplex virus type 1 (HSV-1) virion. The UL37 open reading frame of HSV-1 encodes a 120-kDa virion polypeptide which is a...
SIRT3, a human SIR2 homologue, is an NAD- dependent deacetylase localized to mitochondria
Onyango, Patrick, Celic, Ivana, McCaffery, J. Michael, Boeke, Jef D., Feinberg, Andrew P.
The SIR2 (silent information regulator 2) gene family has diverse functions in yeast including gene silencing, DNA repair, cell-cycle progression, and chromosome fidelity in meiosis and aging. Human...
Inositol pyrophosphates regulate endocytic trafficking
Saiardi, Adolfo, Sciambi, Catherine, McCaffery, J. Michael, Wendland, Beverly, Snyder, Solomon H.
The high energy potential and rapid turnover of the recently discovered inositol pyrophosphates, such as diphosphoinositol-pentakisphosphate and bis-diphosphoinositol-tetrakisphosphate, suggest a...
Walters, Jewell N., Sexton, Gerry L., McCaffery, J. Michael, Desai, Prashant
Protein-protein interactions drive the assembly of the herpes simplex virus type 1 (HSV-1) capsid. A key interaction occurs between the C-terminal tail of the scaffold protein (pre-22a) and the major...
Tor Kinases Are in Distinct Membrane-associated Protein Complexes in Saccharomyces cerevisiae
Wedaman, Karen P., Reinke, Aaron, Anderson, Scott, Yates, John, McCaffery, J. Michael, Powers, Ted
Tor1p and Tor2p kinases, targets of the immune-suppressive antibiotic rapamycin, are components of a highly conserved signaling network that couples nutrient availability and cell growth. To gain...
Aggregation of Expanded Polyglutamine Domain in Yeast Leads to Defects in Endocytosis
Meriin, Anatoli B., Zhang, Xiaoqian, Miliaras, Nicholas B., Kazantsev, Alex, Chernoff, Yury O., McCaffery, J. Michael, ...
The role of aggregation of abnormal proteins in cellular toxicity is of general importance for understanding many neurological disorders. Here, using a yeast model, we demonstrate that mutations in...
Overcoming the hypoxic barrier to radiation therapy with anaerobic bacteria
Bettegowda, Chetan, Dang, Long H., Abrams, Ross, Huso, David L., Dillehay, Larry, Cheong, Ian, ...
The low level of oxygenation within tumors is a major cause of radiation treatment failures. We theorized that anaerobic bacteria that can selectively destroy the hypoxic regions of tumors would...
Huyer, Gregory, Longsworth, Gaby L., Mason, Deborah L., Mallampalli, Monica P., McCaffery, J. Michael, Wright, Robin L., ...
The folding of nascent secretory and membrane proteins is monitored by the endoplasmic reticulum (ER) quality control system. Misfolded proteins are retained in the ER and can be removed by...
Mitochondrial fission proteins regulate programmed cell death in yeast
Fannjiang, Yihru, Cheng, Wen-Chih, Lee, Sarah J., Qi, Bing, Pevsner, Jonathan, McCaffery, J. Michael, ...
The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast...
Functional Analysis of the Triplex Proteins (VP19C and VP23) of Herpes Simplex Virus Type 1
Okoye, Mercy E., Sexton, Gerry L., Huang, Eugene, McCaffery, J. Michael, Desai, Prashant
The triplex of herpesvirus capsids is a unique structural element. In herpes simplex virus type 1 (HSV-1), one molecule of VP19C and two of VP23 form a three-pronged structure that acts to stabilize...
Quenneville, Nicole R., Chao, Tzu-Yuan, McCaffery, J. Michael, Conibear, Elizabeth
Tethering complexes contribute to the specificity of membrane fusion by recognizing organelle features on both donor and acceptor membranes. The Golgi-associated retrograde protein (GARP) complex is...
Zhang, Zijun, McCaffery, J Michael, Spencer, Richard G S, Francomano, Clair A
Cartilage engineering is a strategic experimental goal for the treatment of multiple joint diseases. Based on the process of embryonic chondrogenesis, we hypothesized that cartilage could be...
Kondo-Okamoto, Noriko, Ohkuni, Kentaro, Kitagawa, Katsumi, McCaffery, J. Michael, Shaw, Janet M., Okamoto, Koji
Although it is clear that mitochondrial morphogenesis is a complex process involving multiple proteins in eukaryotic cells, little is known about regulatory molecules that modulate mitochondrial...
Crooks, Denise, Kil, Song Jae, McCaffery, J. Michael, Carlin, Cathleen
Animal cell viruses provide valuable model systems for studying many normal cellular processes, including membrane protein sorting. The focus of this study is an integral membrane protein encoded by...
Clathrin-coated vesicles bearing GAIP possess GTPase-activating protein activity in vitro
Fischer, Thierry, Elenko, Eric, McCaffery, J. Michael, DeVries, Luc, Farquhar, Marilyn Gist
Gα-interacting protein (GAIP) is a member of the RGS (regulators of G protein signaling) family, which serve as GAPs (GTPase-activating proteins) for Gα subunits. Previously, we demonstrated that...
Llopis, Juan, McCaffery, J. Michael, Miyawaki, Atsushi, Farquhar, Marilyn G., Tsien, Roger Y.
Many cellular events depend on a tightly compartmentalized distribution of H+ ions across membrane-bound organelles. However, measurements of organelle pH in living cells have been scarce. Several...
De Vries, Luc, Elenko, Eric, McCaffery, J. Michael, Fischer, Thierry, Hubler, Laura, McQuistan, Tammie, ...
RGS-GAIP (Gα-interacting protein) is a member of the RGS (regulator of G protein signaling) family of proteins that functions to down-regulate Gαi/Gαq-linked signaling. GAIP is a GAP or guanosine...
Division of Mitochondria Requires a Novel DNM1-interacting Protein, Net2p
Cerveny, Kara L., McCaffery, J. Michael, Jensen, Robert E.
Mitochondria are dynamic organelles that undergo frequent division and fusion, but the molecular mechanisms of these two events are not well understood. Dnm1p, a mitochondria-associated,...
Collins, Cynthia S., Kalish, Jennifer E., Morrell, James C., McCaffery, J. Michael, Gould, Stephen J.
Peroxisomes are independent organelles found in virtually all eukaryotic cells. Genetic studies have identified more than 20 PEX genes that are required for peroxisome biogenesis. The role of most...
Localization of the Rsp5p Ubiquitin-Protein Ligase at Multiple Sites within the Endocytic Pathway
Wang, Guangli, McCaffery, J. Michael, Wendland, Beverly, Dupré, Sophie, Haguenauer-Tsapis, Rosine, Huibregtse, Jon M.
The Saccharomyces cerevisiae RSP5 gene encodes an essential HECT E3 ubiquitin-protein ligase. Rsp5p contains an N-terminal C2 domain, three WW domains in the central portion of the molecule, and a...
Oh, SangKon, McCaffery, J. Michael, Eichelberger, Maryna C.
During the acute phase of infection with influenza A virus, the degree of lymphopenia correlates with severity of disease. Factors that contribute to T-cell activation during influenza virus...
Desai, Prashant, Sexton, Gerry L., McCaffery, J. Michael, Person, Stanley
The tegument is an integral and essential structural component of the herpes simplex virus type 1 (HSV-1) virion. The UL37 open reading frame of HSV-1 encodes a 120-kDa virion polypeptide which is a...
SIRT3, a human SIR2 homologue, is an NAD- dependent deacetylase localized to mitochondria
Onyango, Patrick, Celic, Ivana, McCaffery, J. Michael, Boeke, Jef D., Feinberg, Andrew P.
The SIR2 (silent information regulator 2) gene family has diverse functions in yeast including gene silencing, DNA repair, cell-cycle progression, and chromosome fidelity in meiosis and aging. Human...
Inositol pyrophosphates regulate endocytic trafficking
Saiardi, Adolfo, Sciambi, Catherine, McCaffery, J. Michael, Wendland, Beverly, Snyder, Solomon H.
The high energy potential and rapid turnover of the recently discovered inositol pyrophosphates, such as diphosphoinositol-pentakisphosphate and bis-diphosphoinositol-tetrakisphosphate, suggest a...
Walters, Jewell N., Sexton, Gerry L., McCaffery, J. Michael, Desai, Prashant
Protein-protein interactions drive the assembly of the herpes simplex virus type 1 (HSV-1) capsid. A key interaction occurs between the C-terminal tail of the scaffold protein (pre-22a) and the major...
Tor Kinases Are in Distinct Membrane-associated Protein Complexes in Saccharomyces cerevisiae
Wedaman, Karen P., Reinke, Aaron, Anderson, Scott, Yates, John, McCaffery, J. Michael, Powers, Ted
Tor1p and Tor2p kinases, targets of the immune-suppressive antibiotic rapamycin, are components of a highly conserved signaling network that couples nutrient availability and cell growth. To gain...
Aggregation of Expanded Polyglutamine Domain in Yeast Leads to Defects in Endocytosis
Meriin, Anatoli B., Zhang, Xiaoqian, Miliaras, Nicholas B., Kazantsev, Alex, Chernoff, Yury O., McCaffery, J. Michael, ...
The role of aggregation of abnormal proteins in cellular toxicity is of general importance for understanding many neurological disorders. Here, using a yeast model, we demonstrate that mutations in...
Overcoming the hypoxic barrier to radiation therapy with anaerobic bacteria
Bettegowda, Chetan, Dang, Long H., Abrams, Ross, Huso, David L., Dillehay, Larry, Cheong, Ian, ...
The low level of oxygenation within tumors is a major cause of radiation treatment failures. We theorized that anaerobic bacteria that can selectively destroy the hypoxic regions of tumors would...
Huyer, Gregory, Longsworth, Gaby L., Mason, Deborah L., Mallampalli, Monica P., McCaffery, J. Michael, Wright, Robin L., ...
The folding of nascent secretory and membrane proteins is monitored by the endoplasmic reticulum (ER) quality control system. Misfolded proteins are retained in the ER and can be removed by...
Mitochondrial fission proteins regulate programmed cell death in yeast
Fannjiang, Yihru, Cheng, Wen-Chih, Lee, Sarah J., Qi, Bing, Pevsner, Jonathan, McCaffery, J. Michael, ...
The possibility that single-cell organisms undergo programmed cell death has been questioned in part because they lack several key components of the mammalian cell death machinery. However, yeast...
Functional Analysis of the Triplex Proteins (VP19C and VP23) of Herpes Simplex Virus Type 1
Okoye, Mercy E., Sexton, Gerry L., Huang, Eugene, McCaffery, J. Michael, Desai, Prashant
The triplex of herpesvirus capsids is a unique structural element. In herpes simplex virus type 1 (HSV-1), one molecule of VP19C and two of VP23 form a three-pronged structure that acts to stabilize...
Quenneville, Nicole R., Chao, Tzu-Yuan, McCaffery, J. Michael, Conibear, Elizabeth
Tethering complexes contribute to the specificity of membrane fusion by recognizing organelle features on both donor and acceptor membranes. The Golgi-associated retrograde protein (GARP) complex is...
Zhang, Zijun, McCaffery, J Michael, Spencer, Richard G S, Francomano, Clair A
Cartilage engineering is a strategic experimental goal for the treatment of multiple joint diseases. Based on the process of embryonic chondrogenesis, we hypothesized that cartilage could be...
Kondo-Okamoto, Noriko, Ohkuni, Kentaro, Kitagawa, Katsumi, McCaffery, J. Michael, Shaw, Janet M., Okamoto, Koji
Although it is clear that mitochondrial morphogenesis is a complex process involving multiple proteins in eukaryotic cells, little is known about regulatory molecules that modulate mitochondrial...
Mitochondrial mislocalization and altered assembly of a cluster of Barth syndrome mutant tafazzins
Claypool, Steven M., McCaffery, J. Michael, Koehler, Carla M.
None of the 28 identified point mutations in tafazzin (Taz1p), which is the mutant gene product associated with Barth syndrome (BTHS), has a biochemical explanation. In this study, endogenous Taz1p...
Spore Coat Architecture of Clostridium novyi NT Spores▿
Plomp, Marco, McCaffery, J. Michael, Cheong, Ian, Huang, Xin, Bettegowda, Chetan, Kinzler, Kenneth W., ...
Spores of the anaerobic bacterium Clostridium novyi NT are able to germinate in and destroy hypoxic regions of tumors in experimental animals. Future progress in this area will benefit from a better...
Dnm1 forms spirals that are structurally tailored to fit mitochondria
Ingerman, Elena, Perkins, Edward M., Marino, Michael, Mears, Jason A., McCaffery, J. Michael, Hinshaw, Jenny E., ...
Dynamin-related proteins (DRPs) are large self-assembling GTPases whose common function is to regulate membrane dynamics in a variety of cellular processes. Dnm1, which is a yeast DRP (Drp1/Dlp1 in...
Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway
Frederick, Rebecca L., McCaffery, J. Michael, Cunningham, Kyle W., Okamoto, Koji, Shaw, Janet M.
Cell signaling events elicit changes in mitochondrial shape and activity. However, few mitochondrial proteins that interact with signaling pathways have been identified. Candidates include the...
A Mobile PTS2 Receptor for Peroxisomal Protein Import in Pichia pastoris
Elgersma, Ype, Elgersma-Hooisma, Minetta, Wenzel, Thibaut, McCaffery, J. Michael, Farquhar, Marilyn G., Subramani, Suresh
Abstract. Using a new screening procedure for the isolation of peroxisomal import mutants in Pichia pastoris, we have isolated a mutant (pex7) that is specifically disturbed in the peroxisomal import...
The Ran GTPase cycle is required for yeast nuclear pore complex assembly
Ryan, Kathryn J., McCaffery, J. Michael, Wente, Susan R.
Here, we report the first evidence that the Ran GTPase cycle is required for nuclear pore complex (NPC) assembly. Using a genetic approach, factors required for NPC assembly were identified in...
Sekiya-Kawasaki, Mariko, Groen, Aaron Chris, Kaksonen, Marko, Watson, Hadiya A., Zhang, Chao, ...
We used chemical genetics to control the activity of budding yeast Prk1p, which is a protein kinase that is related to mammalian GAK and AAK1, and which targets several actin regulatory proteins...
Wong, Edith D., Wagner, Jennifer A., Gorsich, Steven W., McCaffery, J. Michael, Shaw, Janet M., Nunnari, Jodi
Mutations in the dynamin-related GTPase, Mgm1p, have been shown to cause mitochondrial aggregation and mitochondrial DNA loss in Saccharomyces cerevisiae cells, but Mgm1p's exact role in...
Hobbs, Alyson E. Aiken, Srinivasan, Maithreyan, McCaffery, J. Michael, Jensen, Robert E.
In the yeast Saccharomyces cerevisiae, mitochondria form a branched, tubular reticulum in the periphery of the cell. Mmm1p is required to maintain normal mitochondrial shape and in mmm1 mutants...
Bowman, Aaron B., Patel-King, Ramila S., Benashski, Sharon E., McCaffery, J. Michael, Goldstein, Lawrence S.B., King, Stephen M.
Eukaryotic organisms utilize microtubule-dependent motors of the kinesin and dynein superfamilies to generate intracellular movement. To identify new genes involved in the regulation of axonal...
The Parkinson's disease protein α-synuclein disrupts cellular Rab homeostasis
Gitler, Aaron D., Bevis, Brooke J., Shorter, James, Strathearn, Katherine E., Hamamichi, Shusei, Su, Linhui Julie, ...
α-Synuclein (α-syn), a protein of unknown function, is the most abundant protein in Lewy bodies, the histological hallmark of Parkinson's disease (PD). In yeast α-syn inhibits endoplasmic...
Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast
Jin, Hui, McCaffery, J. Michael, Grote, Eric
Ergosterol depletion independently inhibits two aspects of yeast mating: pheromone signaling and plasma membrane fusion. In signaling, ergosterol participates in the recruitment of Ste5 to a...
Johnson, Brian S., McCaffery, J. Michael, Lindquist, Susan, Gitler, Aaron D.
Protein misfolding is intimately associated with devastating human neurodegenerative diseases, including Alzheimer's, Huntington's, and Parkinson's. Although disparate in their pathophysiology, many...
Claypool, Steven M., Boontheung, Pinmanee, McCaffery, J. Michael, Loo, Joseph A., Koehler, Carla M.
Mutations in the mitochondrial cardiolipin (CL) transacylase, tafazzin (Taz1p), result in the X-linked cardioskeletal myopathy, Barth syndrome (BTHS). The mitochondria of BTHS patients exhibit...
The Yeast LATS/Ndr Kinase Cbk1 Regulates Growth via Golgi-dependent Glycosylation and Secretion
Kurischko, Cornelia, Kuravi, Venkata K., Wannissorn, Nattha, Nazarov, Pavel A., Husain, Michelle, Zhang, Chao, ...
Saccharomyces cerevisiae Cbk1 is a LATS/Ndr protein kinase and a downstream component of the regulation of Ace2 and morphogenesis (RAM) signaling network. Cbk1 and the RAM network are required for...
Mitochondrial outer and inner membrane fusion requires a modified carrier protein
Hoppins, Suzanne, Horner, Jennifer, Song, Cheng, McCaffery, J. Michael, Nunnari, Jodi
In yeast, three proteins are essential for mitochondrial fusion. Fzo1 and Mgm1 are conserved guanosine triphosphatases that reside in the outer and inner membranes, respectively. At each membrane,...
Bcl-xL increases mitochondrial fission, fusion, and biomass in neurons
Berman, Sarah B., Chen, Ying-bei, Qi, Bing, McCaffery, J. Michael, Rucker, Edmund B., Goebbels, Sandra, ...
Mitochondrial fission and fusion are linked to synaptic activity in healthy neurons and are implicated in the regulation of apoptotic cell death in many cell types. We developed fluorescence...
Johnson, Brian S., Snead, David, Lee, Jonathan J., McCaffery, J. Michael, Shorter, James, Gitler, Aaron D.
Mitofusins and OPA1 Mediate Sequential Steps in Mitochondrial Membrane Fusion
Song, Zhiyin, Ghochani, Mariam, McCaffery, J. Michael, Frey, Terrence G., Chan, David C.
Mitochondrial fusion requires the coordinated fusion of the outer and inner membranes. Three large GTPases—OPA1 and the mitofusins Mfn1 and Mfn2—are essential for the fusion of mammalian...