Zhu, Y, Englebert, S, Joris, B, Ghuysen, J M, Kobayashi, T, Lampen, J O
The membrane-spanning protein BlaR is essential for the induction of beta-lactamase in Bacillus licheniformis. Its nature and location were confirmed by the use of an antiserum specific for its...
Zhu, Y F, Curran, I H, Joris, B, Ghuysen, J M, Lampen, J O
The blaR gene of Bacillus licheniformis encodes the signal transducer for induction of the class A beta-lactamase. The protein product, BlaR, has a hydrophilic carboxy region that binds beta-lactams...
Hussain, M, Pastor, F I, Lampen, J O
It has not been clear whether the membrane-bound beta-lactamase III of Bacillus cereus 569 is a separate enzyme or a modified form of the secreted beta-lactamase I. The membrane enzyme is an...
Kobayashi, T, Zhu, Y F, Nicholls, N J, Lampen, J O
A second regulatory locus (blaR1) required for the induction of beta-lactamase synthesis in Bacillus licheniformis 749 was cloned and sequenced. The gene was located on a 5.2-kilobase-pair SphI DNA...
Hussain, M, Carlino, A, Madonna, M J, Lampen, J O
The structural gene for beta-lactamase II (EC 3.5.2.6), a metallothioenzyme, from Bacillus cereus 569/H (constitutive for high production of the enzyme) was cloned in Escherichia coli, and the...
Transcriptional analysis of beta-lactamase regulation in Bacillus licheniformis.
The expression of the blaP gene for the beta-lactamase of Bacillus licheniformis was examined by transcriptional analyses. Radiolabeled probes containing the blaP gene or various regions 3' or 5' to...
Kuhn, H, Fietzek, P P, Lampen, J O
The thermostable, liquefying alpha-amylase from Bacillus licheniformis was immunologically cross-reactive with the thermolabile, liquefying alpha-amylase from Bacillus amyloliquefaciens. Their...
Wang, W, Mézes, P S, Yang, Y Q, Blacher, R W, Lampen, J O
The beta-lactamases of Bacillus cereus have attracted interest because they are secreted efficiently, because multiple enzymes are frequently present, and because their regulation has unusual...
Glyceride-cysteine lipoproteins and secretion by Gram-positive bacteria.
The membrane penicillinases of Bacillus licheniformis and Bacillus cereus are lipoproteins with N-terminal glyceride thioether modification identical to that of the Escherichia coli outer membrane...
Clark, D W, Tkacz, J S, Lampen, J O
The nonspecific alkaline phosphatase of Saccharomyces sp. strain 1710 has been shown by phosphatase cytochemistry to be exclusively located in the vacuole, para-Nitrophenyl phosphate-specific...
Abrams, B B, Hackel, R, Mizunaga, T, Lampen, J O
A mutant strain of Saccharomyces cerevisiae (D10-ER1) has been isolated after a two-step mutagenesis of strain 4059-358D (SUC 1) using ethyl methane sulfonate. Cells of this new strain produced a...
Vesicle penicillinase of Bacillus licheniformis: existence of periplasmic-releasing factor(s).
In earlier studies of the membrane-bound penicillinase of Bacillus licheniformis 749/C, the enzyme present in the vesicles that were released during protoplast formation and the enzyme retained in...
Penicillinase-releasing protease of Bacillus licheniformis: purification and general properties.
Aiyappa, P S, Traficante, L J, Lampen, J O
The membrane penicillinase of Bacillus licheniformis 749/C is a phospholipoprotein which differs from the exoenzyme in that it has an additional sequence of 24 amino acid residues and a...
Ghosh, B. K., Montenecourt, B., Lampen, J. O.
The most obvious morphological characteristic of Saccharomyces mutant FH4C cells is the tendency to form clumps (production of invertase and α-glucosidase by this mutant is highly resistant to...
Ghosh, B. K., Wouters, J. T. M., Lampen, J. O.
Sites of alkaline phosphatase activity have been located by an electron microscopic histochemical (Gomori) technique in vegetative cells of a repressible strain SB15 of Bacillus subtilis, derepressed...
Specific Staining of Wall Mannan in Yeast Cells with Fluorescein-Conjugated Concanavalin A
Tkacz, J. S., Cybulska, E. Barbara, Lampen, J. O.
A procedure is given for the coupling of fluorescein isothiocyanate to concanavalin A, a protein which specifically combines with a variety of polysaccharides, and for the subsequent isolation of the...
Osmotic Regulation of Invertase Formation and Secretion by Protoplasts of Saccharomyces
Kuo, S.-C., Lampen, J. O.
Osmotic regulation of invertase formation and secretion by protoplasts of Saccharomyces was examined using sorbitol, KCl, NaCl, or magnesium sulfate as the osmotic support. The synthesis and...
Sargent, M. G., Ghosh, B. K., Lampen, J. O.
Cultures of the inducible penicillinase-producing strain 749 of Bacillus licheniformis, induced with small amounts of benzylpenicillin, synthesized penicillinase at a high rate for a short period,...
Ghosh, B. K., Lampen, J. O., Remsen, C. C.
Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary...
Kuo, S.-C., Lampen, J. O.
The synthesis of the glycoprotein enzymes, invertase and acid phosphatase, by protoplasts of Saccharomyces mutant 1016, is inhibited by 2-deoxy-d-glucose (2-dG) after a 20- to 30-min lag period under...
Saccharomyces Mutants with Invertase Formation Resistant to Repression by Hexoses
Montenecourt, Bland S., Kuo, S.-C., Lampen, J. O.
Production of invertase by many strains of yeast is repressed in the presence of hexoses. This phenomenon interferes with studies on the secretion of invertase and with the preparation of large...
Chesbro, William R., Lampen, J. O.
The distribution of alkaline phosphatase and nuclease activity between cells and medium was examined in one strain of Bacillus licheniformis and four strains of B. subtilis. Over 95% of both...
Characteristics of Penicillinase Release by Washed Cells of Bacillus licheniformis
Sargent, M. G., Ghosh, B. K., Lampen, J. O.
Saline-washed cells of Bacillus licheniformis strain 749/C (constitutive for penicillinase) were able to release exopenicillinase in the presence of concentrations of chloramphenicol that prevented...
Ghosh, B. K., Sargent, M. G., Lampen, J. O.
Cells of uninduced Bacillus licheniformis (strain 749) in mid-logarithmic phase have no extensive intracytoplasmic membrane. After induction with cephalosporin C, characteristic organelles that...
Localization of Cell-bound Penicillinase in Bacillus licheniformis
Sargent, M. G., Ghosh, B. K., Lampen, J. O.
When protoplasts are prepared from Bacillus licheniformis (strain 749/C, constitutive for penicillinase), approximately 60% of the cell-bound penicillinase is released. The remainder is retained by...
Antibiotic Action of Griseofulvin on Dermatophytes
El-Nakeeb, Moustafa A., McLellan, W. L., Lampen, J. O.
El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), W. L. McLellan, Jr., and J. O. Lampen. Antibiotic action of griseofulvin on dermatophytes. J. Bacteriol. 89:557–563....
Uptake of Griseofulvin by the Sensitive Dermatophyte, Microsporum gypseum
El-Nakeeb, Moustafa A., Lampen, J. O.
El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), and J. O. Lampen. Uptake of griseofulvin by the sensitive dermatophyte, Microsporum gypseum. J. Bacteriol. 89:564–569....
El-Nakeeb, Moustafa A., Lampen, J. O.
El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), and J. O. Lampen. Distribution of griseofulvin taken up by Microsporum gypseum: complexes of the antibiotic with cell...
LOCATION AND ROLE OF STEROL AT NYSTATIN-BINDING SITES
Lampen, J. O., Arnow, Peter M., Borowska, Zofia, Laskin, Allen I.
Lampen, J. O. (Rutgers, The State University, New Brunswick, N.J.), Peter M. Arnow, Zofia Borowska, and Allen I. Laskin. Location and role of sterol at nystatin-binding sites. J. Bacteriol....
INHIBITION OF ALGAE BY NYSTATIN
Lampen, J. O. (Rutgers, the State University, New Brunswick, N. J.), and Peter Arnow. Inhibition of algae by nystatin. J. Bacteriol. 82:247–251. 1961.—The polyenic antibiotic nystatin inhibited...
A Mechanism for Penicillinase Secretion in Bacillus licheniformis*
Cell-bound isozymes of penicillinase are distinguished from extracellular enzyme by their capacity to bind deoxycholate and to elute with an apparent molecular weight of 45,000 on gel filtration in...
Surface Distribution of Invertase on Growing Saccharomyces Cells
By using fluorescein-labeled antibodies, we found that most of the newly formed invertase molecules at the surface of a Saccharomyces cell surround the developing bud.
Protoplasis of Bacillus licheniformis 749/C (a mutant constitutive for penicillinase production) continued to synthesize and release penicillinase in hypertonic growth medium in the presence of...
ABSORPTION OF NYSTATIN BY MICROORGANISMS
Lampen, J. O., Morgan, Elliott R., Slocum, Adelaide, Arnow, Peter
The location of the repressor gene, blaI, for the beta-lactamase gene blaP of Bacillus licheniformis 749, on the 5' side of blaP, was confirmed by sequencing the bla region of the constitutive mutant...
Lipoprotein nature of Bacillus licheniformis membrane penicillinase.
Nielsen, J B, Caulfield, M P, Lampen, J O
Membrane penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) from Bacillus licheniformis bears a striking resemblance to the major outer membrane lipoprotein of Escherichia coli. It can...
Mizunaga, T, Tkacz, J S, Rodriguez, L, Hackel, R A, Lampen, J O
Mutagenesis of the sucrose-fermenting (SUC1) Saccharomyces cerevisiae strain 4059-358D yielded an invertase-negative mutant (D10). Subsequent mutagenic treatment of D10 gave a sucrose-fermenting...
Rodriguez, L, Lampen, J O, MacKay, V L
Saccharomyces cerevisiae revertant strain D10-ER1 has been shown to contain thermosensitive forms of the large (glycoprotein) and small (carbohydrate-free) invertases and a very low level of the...
The membrane penicillinase (EC 3.5.2.6; penicillin amido-beta-lactamhydrolase) of Bacillus licheniforis 749/C, which appears to be an intermediate in the formation of the exoenzyme, is a...
Kuo, S.-C., Cano, F. R., Lampen, J. O.
Lomofungin, an antibiotic active against fungi, yeasts, and bacteria, rapidly inhibits synthesis of ribonucleic acid (RNA) but not protein by protoplasts of Saccharomyces strain 1016. With 40 μg of...
Lomofungin, an Inhibitor of Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerases
Cano, F. R., Kuo, S.-C., Lampen, J. O.
Lomofungin, an antibiotic active against fungi, yeasts, and bacteria, was found to be a potent inhibitor of purified Escherichia coli deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA)...
Secretion by Pseudomonas aeruginosa: fate of a cloned gram-positive lipoprotein deletion mutant.
Nielsen, J B, Mézes, P S, Lampen, J O
In gram-positive organisms, glyceride-cysteine thioether lipoproteins are frequently associated with secretion. They constitute membrane-bound forms retained by the cell but releasable late in growth...
Zhu, Y, Englebert, S, Joris, B, Ghuysen, J M, Kobayashi, T, Lampen, J O
The membrane-spanning protein BlaR is essential for the induction of beta-lactamase in Bacillus licheniformis. Its nature and location were confirmed by the use of an antiserum specific for its...
Zhu, Y F, Curran, I H, Joris, B, Ghuysen, J M, Lampen, J O
The blaR gene of Bacillus licheniformis encodes the signal transducer for induction of the class A beta-lactamase. The protein product, BlaR, has a hydrophilic carboxy region that binds beta-lactams...
Hussain, M, Pastor, F I, Lampen, J O
It has not been clear whether the membrane-bound beta-lactamase III of Bacillus cereus 569 is a separate enzyme or a modified form of the secreted beta-lactamase I. The membrane enzyme is an...
Kobayashi, T, Zhu, Y F, Nicholls, N J, Lampen, J O
A second regulatory locus (blaR1) required for the induction of beta-lactamase synthesis in Bacillus licheniformis 749 was cloned and sequenced. The gene was located on a 5.2-kilobase-pair SphI DNA...
Hussain, M, Carlino, A, Madonna, M J, Lampen, J O
The structural gene for beta-lactamase II (EC 3.5.2.6), a metallothioenzyme, from Bacillus cereus 569/H (constitutive for high production of the enzyme) was cloned in Escherichia coli, and the...
Transcriptional analysis of beta-lactamase regulation in Bacillus licheniformis.
The expression of the blaP gene for the beta-lactamase of Bacillus licheniformis was examined by transcriptional analyses. Radiolabeled probes containing the blaP gene or various regions 3' or 5' to...
Kuhn, H, Fietzek, P P, Lampen, J O
The thermostable, liquefying alpha-amylase from Bacillus licheniformis was immunologically cross-reactive with the thermolabile, liquefying alpha-amylase from Bacillus amyloliquefaciens. Their...
Secretion by Pseudomonas aeruginosa: fate of a cloned gram-positive lipoprotein deletion mutant.
Nielsen, J B, Mézes, P S, Lampen, J O
In gram-positive organisms, glyceride-cysteine thioether lipoproteins are frequently associated with secretion. They constitute membrane-bound forms retained by the cell but releasable late in growth...
Wang, W, Mézes, P S, Yang, Y Q, Blacher, R W, Lampen, J O
The beta-lactamases of Bacillus cereus have attracted interest because they are secreted efficiently, because multiple enzymes are frequently present, and because their regulation has unusual...
Glyceride-cysteine lipoproteins and secretion by Gram-positive bacteria.
The membrane penicillinases of Bacillus licheniformis and Bacillus cereus are lipoproteins with N-terminal glyceride thioether modification identical to that of the Escherichia coli outer membrane...
Clark, D W, Tkacz, J S, Lampen, J O
The nonspecific alkaline phosphatase of Saccharomyces sp. strain 1710 has been shown by phosphatase cytochemistry to be exclusively located in the vacuole, para-Nitrophenyl phosphate-specific...
Abrams, B B, Hackel, R, Mizunaga, T, Lampen, J O
A mutant strain of Saccharomyces cerevisiae (D10-ER1) has been isolated after a two-step mutagenesis of strain 4059-358D (SUC 1) using ethyl methane sulfonate. Cells of this new strain produced a...
Vesicle penicillinase of Bacillus licheniformis: existence of periplasmic-releasing factor(s).
In earlier studies of the membrane-bound penicillinase of Bacillus licheniformis 749/C, the enzyme present in the vesicles that were released during protoplast formation and the enzyme retained in...
Penicillinase-releasing protease of Bacillus licheniformis: purification and general properties.
Aiyappa, P S, Traficante, L J, Lampen, J O
The membrane penicillinase of Bacillus licheniformis 749/C is a phospholipoprotein which differs from the exoenzyme in that it has an additional sequence of 24 amino acid residues and a...
Ghosh, B. K., Montenecourt, B., Lampen, J. O.
The most obvious morphological characteristic of Saccharomyces mutant FH4C cells is the tendency to form clumps (production of invertase and α-glucosidase by this mutant is highly resistant to...
Ghosh, B. K., Wouters, J. T. M., Lampen, J. O.
Sites of alkaline phosphatase activity have been located by an electron microscopic histochemical (Gomori) technique in vegetative cells of a repressible strain SB15 of Bacillus subtilis, derepressed...
Specific Staining of Wall Mannan in Yeast Cells with Fluorescein-Conjugated Concanavalin A
Tkacz, J. S., Cybulska, E. Barbara, Lampen, J. O.
A procedure is given for the coupling of fluorescein isothiocyanate to concanavalin A, a protein which specifically combines with a variety of polysaccharides, and for the subsequent isolation of the...
Osmotic Regulation of Invertase Formation and Secretion by Protoplasts of Saccharomyces
Kuo, S.-C., Lampen, J. O.
Osmotic regulation of invertase formation and secretion by protoplasts of Saccharomyces was examined using sorbitol, KCl, NaCl, or magnesium sulfate as the osmotic support. The synthesis and...
Sargent, M. G., Ghosh, B. K., Lampen, J. O.
Cultures of the inducible penicillinase-producing strain 749 of Bacillus licheniformis, induced with small amounts of benzylpenicillin, synthesized penicillinase at a high rate for a short period,...
Ghosh, B. K., Lampen, J. O., Remsen, C. C.
Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary...
Kuo, S.-C., Lampen, J. O.
The synthesis of the glycoprotein enzymes, invertase and acid phosphatase, by protoplasts of Saccharomyces mutant 1016, is inhibited by 2-deoxy-d-glucose (2-dG) after a 20- to 30-min lag period under...
Saccharomyces Mutants with Invertase Formation Resistant to Repression by Hexoses
Montenecourt, Bland S., Kuo, S.-C., Lampen, J. O.
Production of invertase by many strains of yeast is repressed in the presence of hexoses. This phenomenon interferes with studies on the secretion of invertase and with the preparation of large...
Chesbro, William R., Lampen, J. O.
The distribution of alkaline phosphatase and nuclease activity between cells and medium was examined in one strain of Bacillus licheniformis and four strains of B. subtilis. Over 95% of both...
Characteristics of Penicillinase Release by Washed Cells of Bacillus licheniformis
Sargent, M. G., Ghosh, B. K., Lampen, J. O.
Saline-washed cells of Bacillus licheniformis strain 749/C (constitutive for penicillinase) were able to release exopenicillinase in the presence of concentrations of chloramphenicol that prevented...
Ghosh, B. K., Sargent, M. G., Lampen, J. O.
Cells of uninduced Bacillus licheniformis (strain 749) in mid-logarithmic phase have no extensive intracytoplasmic membrane. After induction with cephalosporin C, characteristic organelles that...
Localization of Cell-bound Penicillinase in Bacillus licheniformis
Sargent, M. G., Ghosh, B. K., Lampen, J. O.
When protoplasts are prepared from Bacillus licheniformis (strain 749/C, constitutive for penicillinase), approximately 60% of the cell-bound penicillinase is released. The remainder is retained by...
Antibiotic Action of Griseofulvin on Dermatophytes
El-Nakeeb, Moustafa A., McLellan, W. L., Lampen, J. O.
El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), W. L. McLellan, Jr., and J. O. Lampen. Antibiotic action of griseofulvin on dermatophytes. J. Bacteriol. 89:557–563....
Uptake of Griseofulvin by the Sensitive Dermatophyte, Microsporum gypseum
El-Nakeeb, Moustafa A., Lampen, J. O.
El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), and J. O. Lampen. Uptake of griseofulvin by the sensitive dermatophyte, Microsporum gypseum. J. Bacteriol. 89:564–569....
El-Nakeeb, Moustafa A., Lampen, J. O.
El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), and J. O. Lampen. Distribution of griseofulvin taken up by Microsporum gypseum: complexes of the antibiotic with cell...
LOCATION AND ROLE OF STEROL AT NYSTATIN-BINDING SITES
Lampen, J. O., Arnow, Peter M., Borowska, Zofia, Laskin, Allen I.
Lampen, J. O. (Rutgers, The State University, New Brunswick, N.J.), Peter M. Arnow, Zofia Borowska, and Allen I. Laskin. Location and role of sterol at nystatin-binding sites. J. Bacteriol....
INHIBITION OF ALGAE BY NYSTATIN
Lampen, J. O. (Rutgers, the State University, New Brunswick, N. J.), and Peter Arnow. Inhibition of algae by nystatin. J. Bacteriol. 82:247–251. 1961.—The polyenic antibiotic nystatin inhibited...
A Mechanism for Penicillinase Secretion in Bacillus licheniformis*
Cell-bound isozymes of penicillinase are distinguished from extracellular enzyme by their capacity to bind deoxycholate and to elute with an apparent molecular weight of 45,000 on gel filtration in...
Surface Distribution of Invertase on Growing Saccharomyces Cells
By using fluorescein-labeled antibodies, we found that most of the newly formed invertase molecules at the surface of a Saccharomyces cell surround the developing bud.
Protoplasis of Bacillus licheniformis 749/C (a mutant constitutive for penicillinase production) continued to synthesize and release penicillinase in hypertonic growth medium in the presence of...
ABSORPTION OF NYSTATIN BY MICROORGANISMS
Lampen, J. O., Morgan, Elliott R., Slocum, Adelaide, Arnow, Peter
The location of the repressor gene, blaI, for the beta-lactamase gene blaP of Bacillus licheniformis 749, on the 5' side of blaP, was confirmed by sequencing the bla region of the constitutive mutant...
Lipoprotein nature of Bacillus licheniformis membrane penicillinase.
Nielsen, J B, Caulfield, M P, Lampen, J O
Membrane penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) from Bacillus licheniformis bears a striking resemblance to the major outer membrane lipoprotein of Escherichia coli. It can...
Mizunaga, T, Tkacz, J S, Rodriguez, L, Hackel, R A, Lampen, J O
Mutagenesis of the sucrose-fermenting (SUC1) Saccharomyces cerevisiae strain 4059-358D yielded an invertase-negative mutant (D10). Subsequent mutagenic treatment of D10 gave a sucrose-fermenting...
Rodriguez, L, Lampen, J O, MacKay, V L
Saccharomyces cerevisiae revertant strain D10-ER1 has been shown to contain thermosensitive forms of the large (glycoprotein) and small (carbohydrate-free) invertases and a very low level of the...
The membrane penicillinase (EC 3.5.2.6; penicillin amido-beta-lactamhydrolase) of Bacillus licheniforis 749/C, which appears to be an intermediate in the formation of the exoenzyme, is a...
Kuo, S.-C., Cano, F. R., Lampen, J. O.
Lomofungin, an antibiotic active against fungi, yeasts, and bacteria, rapidly inhibits synthesis of ribonucleic acid (RNA) but not protein by protoplasts of Saccharomyces strain 1016. With 40 μg of...
Lomofungin, an Inhibitor of Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerases
Cano, F. R., Kuo, S.-C., Lampen, J. O.
Lomofungin, an antibiotic active against fungi, yeasts, and bacteria, was found to be a potent inhibitor of purified Escherichia coli deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA)...