Basal lamina visualization using color image processing and pattern recognition (2005)
Leong, FJWM, Brady, M., McGee, J. O.
In histologic assessment, the absence of basal lamina is a useful feature for distinguishing invasive malignancy from benign and in situ lesions. As this feature is not possible to assess in routine...
Basal lamina visualization using color image processing and pattern recognition (2005)
Leong, FJWM, Brady, M., McGee, J. O.
In histologic assessment, the absence of basal lamina is a useful feature for distinguishing invasive malignancy from benign and in situ lesions. As this feature is not possible to assess in routine...
Chan, V T, Fleming, K A, McGee, J O
A sensitive method for detecting biotinylated DNA probes on dot and Southern blots is described which is based on the principle outlined by Leary et al (1). This system has two main components:...
Bhatt, B, Burns, J, Flannery, D, McGee, J O
A rapid method is described for non isotopic in situ mapping of single copy genes directly on G-banded chromosomes by "one-step" regular light microscopy. It is based on hybridizing biotinylated...
HHV8 and Kaposi's sarcoma: a time cohort study.
Kennedy, M M, Lucas, S B, Jones, R R, Howells, D D, Picton, S J, Hanks, E E, ...
AIMS: The recent finding that human herpes virus 8 (HHV8) is found in the majority of Kaposi's sarcoma (KS) cases supports the epidemiological observation that the tumour may be caused by an...
Identification of HHV8 in early Kaposi's sarcoma: implications for Kaposi's sarcoma pathogenesis.
Kennedy, M M, Cooper, K, Howells, D D, Picton, S, Biddolph, S, Lucas, S B, ...
AIMS: Kaposi's sarcoma is a vascular tumour of uncertain pathogenesis possibly caused by an infectious agent, identified in high risk groups. Accumulating solution phase polymerase chain reaction...
Kennedy, M M, O'Leary, J J, Oates, J L, Lucas, S B, Howells, D D, Picton, S, ...
AIMS: Human herpes virus 8 (HHV-8) is the infectious agent implicated in the pathogenesis of Kaposi's sarcoma, although its mode of action is unclear. Recent work indicates that the HHV-8 genome...
CD40 upregulation is independent of HHV-8 in the pathogenesis of Kaposi's sarcoma.
Kennedy, M M, Biddolph, S, Lucas, S B, Howells, D D, Picton, S, McGee, J O, ...
AIMS: Human herpesvirus 8 (HHV-8) is now acknowledged as the infective cofactor in the pathogenesis of Kaposi's sarcoma. The mode by which HHV-8 causes Kaposi's sarcoma is unresolved and it is...
Prolyl hydroxylase in the skin of patients with obstructive jaundice.
Prolyl hydroxylase was estimated in the skin of 24 jaundiced patients and 25 non-jaundiced patients. The activity of this enzyme was markedly reduced in the skin of the jaundiced patients.
Distribution of HLA class 1 antigens in normal human tissue and in mammary cancer.
Fleming, K A, McMichael, A, Morton, J A, Woods, J, McGee, J O
With a monoclonal antibody which reacts with all HLA class 1 antigens it was found that these antigens are not uniformly distributed in all nucleated cells. Rather HLA class 1 antigens are restricted...
Cooper, K, Herrington, C S, Stickland, J E, Evans, M F, McGee, J O
It was postulated that non-isotopic in situ hybridisation (NISH) signal types 1-3 for human papillomavirus in cervical biopsy specimens represent episomal or integrated virus. The aim of this study...
Troncone, G, Herrington, C S, Cooper, K, De Angelis, M L, McGee, J O
AIMS: To determine the relative diagnostic sensitivity of non-isotopic in situ hybridisation (NISH) for the diagnosis of human papillomavirus (HPV) on matched smears and biopsy specimens; to compare...
Integration of human papillomavirus types 16 and 18 in cervical adenocarcinoma.
Cooper, K, Herrington, C S, Lo, E S, Evans, M F, McGee, J O
AIMS: To determine which type of human papillomavirus (HPV) is associated with cervical adenocarcinoma and whether the virus was integrated or episomal in two continents. METHODS: Biopsy specimens...
Detection of high risk human papillomavirus in routine cervical smears: strategy for screening.
Herrington, C S, De Angelis, M, Evans, M F, Troncone, G, McGee, J O
AIM: To develop a methodology for direct detection of high risk human papillomavirus (HPV) infection in routine cervical smears by non-isotopic in situ hybridisation (NISH) which can be compared with...
Martinez-Montero, J C, Herrington, C S, Stickland, J, Sawyer, H, Evans, M, Flannery, D M, ...
The aim of this study was to optimise conditions for mRNA detection by nonisotopic in situ hybridisation (NISH) using biotinylated and digoxigenin labelled riboprobes. Because lysozyme gene...
Cooper, K, Herrington, C S, Graham, A K, Evans, M F, McGee, J O
In South Africa asymptomatic wart virus infection diagnosed by morphological criteria occurs in 16-20% of all ethnic groups; the incidence in black women is 66%. To identify human papillomavirus...
Cooper, K, Herrington, C S, Graham, A K, Evans, M F, McGee, J O
In a previous study three types of HPV signal were described in CIN. It was suggested that a type 1 signal represented episomal HPV while a type 2 signal represented integrated HPV; and a type 3...
Herrington, C S, Graham, A K, McGee, J O
A monoclonal antibody to digoxin enabled sandwich techniques to be used for the detection of hybridised digoxigenin labelled probes in cultured cells and paraffin wax sections. This system has...
Isolation of collagen stimulating factors from healing wounds.
O'Hare, R P, Fallon, A, Bradley, J F, Burns, J, McGee, J O
Five factors (collagen stimulating factors) have been isolated from healing murine skin wounds which stimulate prolyl hydroxylase activity and collagen synthesis in mouse fibroblasts in vitro. These...
Burns, J, D'Ardenne, A J, Morton, J A, McGee, J O
A Mallory body (alcoholic hyaline) antigen (JMB2) which is also present in intermediate filaments of epithelial origin was demonstrated immunohistochemically in renal glomeruli of three out of eleven...
Alcohol induced liver disease.
Alcohol induces a variety of changes in the liver: fatty change, hepatitis, fibrosis, and cirrhosis. The histopathological appearances of these conditions are discussed, with special attention to...
Giant cell myocarditis: evidence for the macrophage origin of the giant cells.
Theaker, J M, Gatter, K C, Heryet, A, Evans, D J, McGee, J O
In order to elucidate the origin of giant cells in giant cell myocarditis a case has been studied immunohistologically using monoclonal antibodies against a variety of antigens, including those...
Vascular occlusion and infarction in sickle cell crisis and the sickle chest syndrome.
Athanasou, N A, Hatton, C, McGee, J O, Weatherall, D J
A young adult with homozygous sickle cell anaemia (Hb SS) suffered a fatal sickle cell crisis complicated by the sickle chest syndrome. At necropsy multiple large infarcts of the lung, bone marrow,...
Histological features of sclerosing cholangitis in patients with chronic ulcerative colitis.
Barbatis, C, Grases, P, Shepherd, H A, Chapman, R W, Trowell, J, Jewell, D P, ...
Primary sclerosing cholangitis was diagnosed radiologically in 16 of 681 patients (2.2%) with chronic ulcerative colitis in a follow up study at the gastroenterology unit in Oxford. On the basis of...
Burns, J, Chan, V T, Jonasson, J A, Fleming, K A, Taylor, S, McGee, J O
A fast and sensitive method for detecting biotinylated deoxyribonucleic acid (DNA) probes was used for sex determination of cells and tissues by in situ hybridisation of a probe "specific" for the Y...
Monoclonal antibody to macrophages (EMB/11) labels macrophages and microglial cells in human brain.
Normal and diseased human central nervous system (CNS) tissues were studied immunohistochemically by a monoclonal antibody to human macrophages (EBM/11), antisera to glial fibrillary acidic protein...
Burns, J, Redfern, D R, Esiri, M M, McGee, J O
A simple reproducible protocol for detecting multiple copy human genes and viral DNA in routine formalin fixed paraffin embedded tonsil and brain, by in situ hybridisation with biotinylated probes,...
Cyclin D1 expression and HHV8 in Kaposi sarcoma.
Kennedy, M M, Biddolph, S, Lucas, S B, Howells, D D, Picton, S, McGee, J O, ...
BACKGROUND: Human herpesvirus 8 (HHV8) appears to be the agent responsible for Kaposi sarcoma. The mechanism remains undetermined but may involve cell cycle regulating genes including D type cyclins...
Role of human papillomavirus in determining the HLA associated risk of cervical carcinogenesis.
Mehal, W Z, Lo, Y M, Herrington, C S, Evans, M F, Papadopoulos, M C, Odunis, K, ...
AIMS--To investigate the role of human papillomavirus (HPV) in the association between HLA DQw3 and squamous cell cancer of the cervix (SCCC). METHODS--Tissue from 194 cervical samples, ranging from...
Herrington, C S, Anderson, S M, Bauer, H M, Troncone, B, De Angelis, M L, Noell, H, ...
AIMS--To assess the relative diagnostic performance of the polymerase chain reaction (PCR) and non-isotopic in situ hybridisation (NISH) and to correlate these data with cytopathological assessment....
Loss of heterozygosity on chromosome 11 q in breast cancer.
Tomlinson, I P, Strickland, J E, Lee, A S, Bromley, L, Evans, M F, Morton, J, ...
AIMS--Chromosome 11q23 seems to be a site of frequent mutation in cancer. It also contains loci such as ataxia telangiectasia with possible importance in the pathogenesis of breast tumours. The short...
Greywoode, G I, McCarthy, S P, McGee, J O
When first reported, EBM/11 reacted with mononuclear phagocyte system cells only in fresh frozen sections, but it has been shown to have similar cellular specificity in routine formalin fixed,...
Burns, J, Graham, A K, Frank, C, Fleming, K A, Evans, M F, McGee, J O
In analysing human papilloma virus (HPV) infection of the cervix in formalin fixed paraffin sections by non-isotopic in situ hybridisation two main problems were found: detachment of sections from...
Effect of decalcification agents on immunoreactivity of cellular antigens.
Athanasou, N A, Quinn, J, Heryet, A, Woods, C G, McGee, J O
The effects of several strong acids, weak acids, a proprietary decalcifier, and edetic acid on the immunohistochemical staining of cryostat and fixed paraffin embedded sections of tissue from a...
Immunocytochemical analysis of HLA class II (DR) antigens in liver disease in man.
Barbatis, C, Kelly, P, Greveson, J, Heryet, A, McGee, J O
The in situ distribution of the major histocompatibility (HLA) class II (DR) antigens was studied in 113 liver biopsy specimens and five livers obtained at necropsy, using monoclonal antibody CR3/43....
Cellular oncogenes in neoplasia.
In recent years cellular homologues of many viral oncogenes have been identified. As these genes are partially homologous to viral oncogenes and are activated in some tumour cell lines they are...
Immunoperoxidase staining of intermediate filaments in human small bowel.
Malik, A K, Rawcliffe, P, Morton, J A, Jewell, D P, McGee, J O
Monoclonal antibody EBM/11: high cellular specificity for human macrophages.
Kelly, P M, Bliss, E, Morton, J A, Burns, J, McGee, J O
A monoclonal antibody, EBM/11, was raised against isolated human lung macrophages. Immunohistochemically this antibody reacted with freshly isolated lung macrophages and blood monocytes, mononuclear...
Non-isotopic detection of in situ nucleic acid in cervix: an updated protocol.
Burns, J, Graham, A K, McGee, J O
Recently, sensitive non-isotopic in situ hybridisation (NISH) methodology for the detection of human DNA and human papilloma virus (HPV) DNA in archival paraffin blocks of cervix was described. An...
EBM/11 reactivity in malignant histiocytosis.
Kelly, P M, McGovern, M, Gatter, K C, Theaker, J M, McGee, J O
A patient presented initially with a testicular mass, which on biopsy had morphological features consistent with malignant histiocytosis. The tumour cells labelled strongly with EBM/11, a murine...
Herrington, C S, Burns, J, Graham, A K, Evans, M, McGee, J O
This study was undertaken to develop technology for the detection of nucleic acid using two different DNA probe reporter molecules, the ultimate aim being to differentially label two nucleic acids...
Herrington, C S, Burns, J, Graham, A K, Bhatt, B, McGee, J O
A method was developed for the simultaneous detection of viral and human DNA in contrasting colours in routine formalin fixed, paraffin wax embedded biopsy specimens. This was achieved by...
Distribution of fibronectin in normal and diseased human kidneys.
Dixon, A J, Burns, J, Dunnill, M S, McGee, J O
The distribution of fibronectin in 42 renal biopsies was investigated using an indirect immunoperoxidase technique on paraffin sections of formalin-fixed material. Biopsies were obtained from normal...
The relationship between collagen and C1q biosynthesis in cultured human fibroblasts.
The relationship between collagen and C1q biosynthesis has been investigated in cultured human fibroblasts. This was done by measuring the effects of variation in cell density, inhibition of prolyl...
Raised gamma-glutamyltransferase activity and the need for liver biopsy.
Ireland, A, Hartley, L, Ryley, N, McGee, J O, Trowell, J M, Chapman, R W
Chan, V T, Fleming, K A, McGee, J O
A sensitive method for detecting biotinylated DNA probes on dot and Southern blots is described which is based on the principle outlined by Leary et al (1). This system has two main components:...
Bhatt, B, Burns, J, Flannery, D, McGee, J O
A rapid method is described for non isotopic in situ mapping of single copy genes directly on G-banded chromosomes by "one-step" regular light microscopy. It is based on hybridizing biotinylated...
HHV8 and Kaposi's sarcoma: a time cohort study.
Kennedy, M M, Lucas, S B, Jones, R R, Howells, D D, Picton, S J, Hanks, E E, ...
AIMS: The recent finding that human herpes virus 8 (HHV8) is found in the majority of Kaposi's sarcoma (KS) cases supports the epidemiological observation that the tumour may be caused by an...
Identification of HHV8 in early Kaposi's sarcoma: implications for Kaposi's sarcoma pathogenesis.
Kennedy, M M, Cooper, K, Howells, D D, Picton, S, Biddolph, S, Lucas, S B, ...
AIMS: Kaposi's sarcoma is a vascular tumour of uncertain pathogenesis possibly caused by an infectious agent, identified in high risk groups. Accumulating solution phase polymerase chain reaction...
Kennedy, M M, O'Leary, J J, Oates, J L, Lucas, S B, Howells, D D, Picton, S, ...
AIMS: Human herpes virus 8 (HHV-8) is the infectious agent implicated in the pathogenesis of Kaposi's sarcoma, although its mode of action is unclear. Recent work indicates that the HHV-8 genome...
CD40 upregulation is independent of HHV-8 in the pathogenesis of Kaposi's sarcoma.
Kennedy, M M, Biddolph, S, Lucas, S B, Howells, D D, Picton, S, McGee, J O, ...
AIMS: Human herpesvirus 8 (HHV-8) is now acknowledged as the infective cofactor in the pathogenesis of Kaposi's sarcoma. The mode by which HHV-8 causes Kaposi's sarcoma is unresolved and it is...
Prolyl hydroxylase in the skin of patients with obstructive jaundice.
Prolyl hydroxylase was estimated in the skin of 24 jaundiced patients and 25 non-jaundiced patients. The activity of this enzyme was markedly reduced in the skin of the jaundiced patients.
Distribution of HLA class 1 antigens in normal human tissue and in mammary cancer.
Fleming, K A, McMichael, A, Morton, J A, Woods, J, McGee, J O
With a monoclonal antibody which reacts with all HLA class 1 antigens it was found that these antigens are not uniformly distributed in all nucleated cells. Rather HLA class 1 antigens are restricted...
Cooper, K, Herrington, C S, Stickland, J E, Evans, M F, McGee, J O
It was postulated that non-isotopic in situ hybridisation (NISH) signal types 1-3 for human papillomavirus in cervical biopsy specimens represent episomal or integrated virus. The aim of this study...
Troncone, G., Herrington, C. S., Cooper, K., De Angelis, M. L., McGee, J. O.
AIMS: To determine the relative diagnostic sensitivity of non-isotopic in situ hybridisation (NISH) for the diagnosis of human papillomavirus (HPV) on matched smears and biopsy specimens; to compare...
Integration of human papillomavirus types 16 and 18 in cervical adenocarcinoma.
Cooper, K., Herrington, C. S., Lo, E. S., Evans, M. F., McGee, J. O.
AIMS: To determine which type of human papillomavirus (HPV) is associated with cervical adenocarcinoma and whether the virus was integrated or episomal in two continents. METHODS: Biopsy specimens...
Detection of high risk human papillomavirus in routine cervical smears: strategy for screening.
Herrington, C. S., De Angelis, M., Evans, M. F., Troncone, G., McGee, J. O.
AIM: To develop a methodology for direct detection of high risk human papillomavirus (HPV) infection in routine cervical smears by non-isotopic in situ hybridisation (NISH) which can be compared with...