Population biology of Toxoplasmagondii : what’s out and where did they come from (2009)
Until recently, Toxoplasmagondii was considered to be clonal with little genetic variability. In this paper we summarize recent genotyping data from chickens in Brazil, and pigs, lambs and...
Bontell, I.L., Hall, N., Ashelford, K.E., Dubey, J.P., Boyle, J.P., Lindh, J., ...
BACKGROUND Toxoplasma gondii is a zoonotic parasite of global importance. In common with many protozoan parasites it has the capacity for sexual recombination, but current evidence suggests this is...
Seroprevalence and Isolation of Toxoplasma gondii From Sheep From São Paulo State, Brazil (2008)
L. N. Oliveira, R. A. Dias, J. P. Dubey, S. M. Gennari
Sheep are important in the epidemiology of Toxoplasma gondii infection, but little is known of ovine toxoplasmosis in Brazil. Antibodies to T. gondii were assayed in sera of 495 sheep from 36...
Fatal Intestinal Coccidiosis in a Three-Week-Old Buffalo Calf (Bubalus bubalus) (2008)
J. P. Dubey, W. Wouda, J. Muskens
The water buffalo (Bubalus bubalus) is important to the economy of several countries, especially in Asia and South America. Little is known regarding the impact of coccidiosis in buffaloes. Cattle...
Lethal Toxoplasmosis in an Aviary of Kakarikis (Cyanoramphus Spp.) in Australia (2008)
J. Hartley, R. Booth, R. F. Slocombe, J. P. Dubey
A small outbreak of fatal toxoplasmosis is described in 6 kakariki (Cyanoramphus spp.) in an aviary in Australia. Toxoplasma gondii-like tachyzoites were found associated with necrosis in intestine,...
Daniel M. Aguiar, José T. Damaceno, Luciana N. Oliveira, J. P. Dubey, ...
The capybara (Hydrochaeris hydrochaeris) is a large rodent used for human consumption in certain areas of South America. In the present study, viable Toxoplasma gondii was isolated for the first time...
Most species of felids tested have been found to be the definitive host for Toxoplasma gondii. Gordon's wildcat (Felis silvestris gordoni) is a threatened species found in the United Arab Emirates...
S. Sharma, K. S. Sandhu, M. S. Bal, H. Kumar, S. Verma, J. P. Dubey
Sera from 186 sheep, 83 cattle, and 103 water buffaloes from Punjab, India, were evaluated for antibodies to Toxoplasma gondii using a commercial ELISA kit. This study was planned using a 2-stage...
Fatal Toxoplasmosis in Sand Cats (Felis margarita) (2008)
The sand cat (Felis margarita) is a small-sized felid occurring in the United Arab Emirates (UAE). The sand cat captive-breeding program at the Breeding Centre for Endangered Arabian Wildlife in...
C. Alvarado-Esquivel, R. Esquivel-Cruz, S. Estrada-Martínez, M. Rivas-González, O. Liesenfeld, ...
There is scarce information concerning the epidemiology of Toxoplasma gondii infection in people of rural Mexico. Anti-T. gondii immunoglobulin (Ig)G and IgM antibodies were sought in 462 adult...
Isolation of Toxoplasma gondii From Bottlenose Dolphins (Tursiops truncatus) (2008)
J. P. Dubey, P. A. Fair, N. Sundar, G. Velmurugan, W. E. McFee, ...
Toxoplasma gondii infection in marine mammals is intriguing and indicative of contamination of the ocean environment and coastal waters with oocysts. In previous serological surveys, >90% of...
Australasian marsupials, especially wallabies, are highly susceptible to clinical toxoplasmosis. This paper describes the use of atovaquone for effective treatment of toxoplasmosis in 4 Bennett's...
Toxoplasmosis in Sand Fox (Vulpes rueppelli) (2008)
Fatal toxoplasmosis was diagnosed in a sand fox (Vulpes rueppelli) from United Arab Emirates. Toxoplasma gondii–like tachyzoites were found associated with necrosis in the intestine, spleen, liver,...
Toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to T....
Toxoplasma gondii and Neospora caninum Antibodies in Dogs From Grenada, West Indies (2008)
J. P. Dubey, D. Stone, R. N. Sharma
Toxoplasma gondii and Neospora caninum are structurally similar parasites with many common hosts. The prevalence of antibodies to T. gondii and N. caninum was determined in sera from dogs in Grenada,...
Effects of High Pressure Processing on Toxoplasma gondii Oocysts on Raspberries (2008)
David S. Lindsay, Daniel Holliman, George J. Flick, David G. Goodwin, Sheila M. Mitchell, J. P. Dubey
Oocysts are the environmentally resistant life stage of Toxoplasma gondii. Humans can become infected by accidentally ingesting the oocysts in water or from contaminated produce. Severe disease can...
Experimental Toxoplasma gondii Infection in Striped Skunk (Mephitis mephitis) (2008)
Twenty-three striped skunks (Mephitis mephitis) without demonstrable antibodies in 1:25 serum dilution in the modified agglutination test (MAT) were fed sporulated Toxoplasma gondii oocysts (9...
Prevalence of Antibodies to Toxoplasma gondii in Wolverines From Nunavut, Canada (2008)
Mason V. Reichard, Luigi Torretti, Jason M. Garvon, J. P. Dubey
The prevalence of antibodies to Toxoplasma gondii was determined in blood and tissue exudates recovered from the spleens of 41 wolverines (Gulo gulo) collected in Nunavut, Canada, using a modified...
Capybara (Hydrochaeris hydrochaeris) is a large rodent distributed throughout tropical America. Antibodies to Neospora caninum in 213 feral capybaras from 11 counties of the State of São Paulo,...
Toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to T....
Toxoplasma gondii and Neospora caninum Antibodies in Dogs From Grenada, West Indies (2008)
J. P. Dubey, D. Stone, R. N. Sharma
Toxoplasma gondii and Neospora caninum are structurally similar parasites with many common hosts. The prevalence of antibodies to T. gondii and N. caninum was determined in sera from dogs in Grenada,...
Effects of High Pressure Processing on Toxoplasma gondii Oocysts on Raspberries (2008)
David S. Lindsay, Daniel Holliman, George J. Flick, David G. Goodwin, Sheila M. Mitchell, J. P. Dubey
Oocysts are the environmentally resistant life stage of Toxoplasma gondii. Humans can become infected by accidentally ingesting the oocysts in water or from contaminated produce. Severe disease can...
Experimental Toxoplasma gondii Infection in Striped Skunk (Mephitis mephitis) (2008)
Twenty-three striped skunks (Mephitis mephitis) without demonstrable antibodies in 1:25 serum dilution in the modified agglutination test (MAT) were fed sporulated Toxoplasma gondii oocysts (9...
Prevalence of Antibodies to Toxoplasma gondii in Wolverines From Nunavut, Canada (2008)
Mason V. Reichard, Luigi Torretti, Jason M. Garvon, J. P. Dubey
The prevalence of antibodies to Toxoplasma gondii was determined in blood and tissue exudates recovered from the spleens of 41 wolverines (Gulo gulo) collected in Nunavut, Canada, using a modified...
Capybara (Hydrochaeris hydrochaeris) is a large rodent distributed throughout tropical America. Antibodies to Neospora caninum in 213 feral capybaras from 11 counties of the State of São Paulo,...
J. P. Dubey, J. G. Humphreys, D. Fritz
Infection with Sarcocystis species is common in herbivores but is rare in bears. Histological sections of 374 black bears (Ursus americanus) from Pennsylvania were examined for sarcocysts. In total,...
Novel Triazine JPC-2067-B Inhibits Toxoplasma gondii In Vitro and In Vivo (2008)
Mui, E.J., Schiehser, G.A., Milhous, W.K., Hsu, H., Roberts, C.W., Kirisits, M., ...
BACKGROUND AND METHODOLOGY Toxoplasma gondii causes substantial morbidity, mortality, and costs for healthcare in the developed and developing world. Current medicines are not well tolerated and...
Seroprevalence of Neospora caninum Antibodies in Dogs in India (2008)
S. Sharma, M. S. Bal, K. Kaur, K. S. Sandhu, J. P. Dubey
Neospora caninum is one of most important causes of abortion in cattle worldwide, and dogs are an important risk factor for N. caninum infection in cattle. Antibodies to N. caninum were determined in...
J. P. Dubey, D. E. Hill, N. Sundar, G. V. Velmurugan, L. A. Bandini, ...
The prevalence of Toxoplasma gondii was investigated on a poorly managed pig farm in Maryland. Serum and tissue samples from 48 of the 100 pigs on the farm were available for T. gondii evaluation....
J. P. Dubey, T. Quirk, J. A. Pitt, N. Sundar, G. V. Velmurugan, ...
Viable Toxoplasma gondii was isolated by bioassay in mice from tissues of 2 feral cats (Felis domesticus), 2 raccoons (Procyon lotor), a skunk (Mephitis mephitis) trapped in remote locations in...
J. P. Dubey, D. T. Subekti, P. Tassi, W. Cabaj, ...
The prevalence of Toxoplasma gondii in free-ranging chickens (Gallus domesticus) is a good indicator of the prevalence of the parasite's oocysts in soil because chicken feed from the ground. The...
MOUSE-VIRULENT TOXOPLASMA GONDII ISOLATED FROM FERAL CATS ON MONA ISLAND, PUERTO RICO (2007)
J. P. Dubey, N. Sundar, G. V. Velmurugan, D. Ajzenberg, ...
Cats are essential in the life cycle of Toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts. Samples of serum, feces, and tissues from cats from...
SEROPREVALENCE OF TOXOPLASMA GONDII IN MESOCARNIVORES OF THE CANADIAN PRAIRIES (2007)
Y. T. Hwang, J. A. Pitt, T. W. Quirk, J. P. Dubey
The protozoon Toxoplasma gondii has a worldwide distribution and affects many species of warm-blooded animals. In the Canadian prairies, mesocarnivores such as striped skunks (Mephitis mephitis) and...
SEROPREVALENCE OF NEOSPORA CANINUM ANTIBODIES IN CATTLE AND WATER BUFFALOES IN INDIA (2007)
K. S. Sandhu, M. S. Ball, H. Kumar, S. Sharma, P. K. Sidhu, ...
Neospora caninum is now recognized as a major cause of abortion in cattle worldwide, but there is no report of N. caninum infection in cattle in India. Serum samples from 427 dairy cattle and 32...
J. P. Dubey, N. Sundar, C. A. Nolden, M. D. Samuel, G. V. Velmurugan, L. A. Bandini, ...
During 2005–2006, sera and tissues from raccoons (Procyon lotor), coyotes (Canis latrans), and skunks (Mephitis mephitis) from the state of Wisconsin were tested for Toxoplasma gondii infection....
NEOSPORA CANINUM AND TOXOPLASMA GONDII ANTIBODIES IN DOGS FROM DURANGO CITY, MEXICO (2007)
J. P. Dubey, C. Alvarado-Esquivel, O. Liesenfeld, A. González-Herrera, ...
Seroprevalence of Toxoplasma gondii Antibodies in Cats From Durango City, Mexico (2007)
C. Alvarado-Esquivel, O. Liesenfeld, A. González-Herrera, ...
Early Migration of Sarcocystis neurona in Ponies Fed Sporocysts (2007)
E. Elitsur, A. E. Marsh, S. M. Reed, J. P. Dubey, M. J. Oglesbee, J. E. Murphy, ...
Seroprevalence of Toxoplasma gondii in Pigs From Vietnam (2007)
Pigs are considered an important source of Toxoplasma gondii infection for humans. Antibodies to T. gondii were determined in serum samples from 587 pigs from Vietnam using the modified agglutination...
Seroprevalence of Toxoplasma gondii in Cats From St. Kitts, West Indies (2007)
L. Moura, P. Kelly, R. C. Krecek, J. P. Dubey
The prevalence of antibodies to Toxoplasma gondii was determined in sera from 106 domestic cats from St. Kitts, West Indies. Using a modified agglutination test, antibodies to this parasite were...
J. P. Dubey, J. A. Morales, N. Sundar, G. V. Velmurugan, G. Hernández-Mora, ...
Toxoplasma gondii infection in marine mammals is of interest because of mortality and mode of transmission. It has been suggested that marine mammals become infected with T. gondii oocysts washed...
DIVERSE AND ATYPICAL GENOTYPES IDENTIFIED IN TOXOPLASMA GONDII FROM DOGS IN SÃO PAULO, BRAZIL (2007)
Neosporosis and hammondiosis in dogs (2007)
Reichel, Michael Philipp, Ellis, John T., Dubey, J. P.
The dog is a definitive host of the protozoan parasite Neospora caninum, and in many parts of the world, infection is relatively common as determined by serology. Reported seroprevalences usually...
Neosporosis and hammondiosis in dogs (2007)
Reichel, Michael Philipp, Ellis, John T., Dubey, J. P.
The dog is a definitive host of the protozoan parasite Neospora caninum, and in many parts of the world, infection is relatively common as determined by serology. Reported seroprevalences usually...
Neosporosis and hammondiosis in dogs (2007)
Reichel, Michael Philipp, Ellis, John T., Dubey, J. P.
The dog is a definitive host of the protozoan parasite Neospora caninum, and in many parts of the world, infection is relatively common as determined by serology. Reported seroprevalences usually...
Seroprevalence of Toxoplasma gondii in Pregnant Women and Cats in Grenada, West Indies (2006)
Sumita P. Asthana, Stanley H. Weiss, Richard Stephens, Thomas N. Denny, R. N. Sharma, ...
Seroprevalence of Toxoplasma gondii Antibodies in Humans From Rural Western Amazon, Brazil (2006)
G. T. Cavalcante, D. M. Aguiar, M. B. Labruna, L. R. Meireles, ...
Characterization of Toxoplasma gondii Isolates in Free-Range Chickens From Portugal (2006)
J. P. Dubey, S. Sousa, N. Canada, S. Meireles, ...
Effects of High-Pressure Processing on Toxoplasma gondii Tissue Cysts in Ground Pork (2006)
David S. Lindsay, Marina V. Collins, Daniel Holliman, George J. Flick, J. P. Dubey
CHARACTERIZATION OF TOXOPLASMA GONDII ISOLATES IN FREE-RANGE CHICKENS FROM AMAZON, BRAZIL (2006)
J. P. Dubey, Solange M. Gennari, Marcelo B. Labruna, Paula L. Marcet, ...
J. P. Dubey, A. Lenhart, C. E. Castillo, L. Alvarez, P. Marcet, C. Sreekumar, ...
The prevalence of Toxoplasma gondii, in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii...
CHARACTERIZATION OF TOXOPLASMA GONDII ISOLATES IN FREE-RANGE CHICKENS FROM ARGENTINA (2005)
J. P. Dubey, Paula L. Marcet, T. Lehmann
The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii...
Isolation and Molecular Characterization of Toxoplasma gondii From Chickens From Sri Lanka (2005)
J. P. Dubey, D. K. Ekanayake, C. Sreekumar, T. Lehmann
The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii...
David S. Lindsay, Taranjit Kaur, Sheila M. Mitchell, David G. Goodwin, Jeannine Strobl, J. P. Dubey
Groups of mice were infected with tachyzoites of the RH strain of Toxoplasma gondii, treated with the opioid analgesic buprenorphine, sodium sulfadiazine, a combination of buprenorphine and sodium...
J. P. Dubey, P. A. Fair, G. D. Bossart, D. Hill, R. Fayer, C. Sreekumar, ...
Toxoplasma gondii infection in marine mammals is intriguing and indicative of contamination of the ocean environment and coastal waters with oocysts. In a previous study, 138 of 141 (97.8%)...
J. P. Dubey, D. E. Hill, J. L. Jones, A. W. Hightower, E. Kirkland, J. M. Roberts, ...
The prevalence of viable Toxoplasma gondii was determined in 6,282 samples (2,094 each of beef, chicken, and pork) obtained from 698 retail meat stores from 28 major geographic areas of the United...
Prevalence of Antibodies to Neospora caninum in Wild Animals (2005)
Antibodies to Neospora caninum were determined in several species of wild animals in the United States by the Neospora agglutination test (NAT). Antibodies (NAT 1:40 or higher) were found in 5 of 249...
J. P. Dubey, B. Lopez, M. Alvarez, C. Mendoza, T. Lehmann
The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii...
J. P. Dubey, M. I. Bhaiyat, C. De Allie, R. N. Sharma, C. Sreekumar, ...
The prevalence of Toxoplasma gondii in free-range chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in...
NEOSPORA CANINUM ANTIGENS DEFINED BY ANTIGEN-DEPENDENT BOVINE CD4+ T CELLS (2005)
Wenbin Tuo, Raymond H. Fetterer, William C. Davis, Mark C. Jenkins, J. P. Dubey
Neosporosis is an important cause of pregnancy loss in cattle worldwide. The objective of the present study was to identify Neospora caninum antigens as vaccine candidates using antigen-specific,...
Toxoplasmosis in a Hawaiian Monk Seal (Monachus schauinslandi ) (2005)
Shelley P. Honnold, Robert Braun, Dana P. Scott, C. Sreekumar, J. P. Dubey
Toxoplasma gondii infection in marine mammals is intriguing and indicative of contamination of the ocean environment with oocysts. T. gondii was identified in a Hawaiian monk seal (Monachus...
Effects of High Pressure Processing on Infectivity of Toxoplasma gondii Oocysts for Mice (2005)
David S. Lindsay, Marina V. Collins, Carly N. Jordan, George J. Flick, J. P. Dubey
High pressure processing (HPP) has been shown to be an effective non-thermal method of eliminating non-spore forming bacteria from a variety of food products. The shelf-life of the products is...
Clinical Muscular Sarcocystosis in a Dog (2005)
J. Chapman, M. Mense, J. P. Dubey
Muscular sarcocystosis is a rare infection in dogs. Clinical myositis associated with an unidentified species of Sarcocystis was diagnosed in an adult dog from Canada. There was granulomatous...
J. P. Dubey, S. Karhemere, E. Dahl, C. Sreekumar, A. Diabaté, K. R. Dabiré, ...
The prevalence of Toxoplasma gondii in free-ranging chickens (Gallus domesticus) is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. In the...
Khan, Asis, Taylor, Sonya, Su, Chunlei, Mackey, Aaron J., Boyle, Jon, Cole, Robert, ...
Toxoplasma gondii is a highly successful protozoan parasite in the phylum Apicomplexa, which contains numerous animal and human pathogens. T.gondii is amenable to cellular, biochemical, molecular and...
C. D. Sofaly, S. M. Reed, J. P. Dubey, M. J. Oglesbee, V. A. Lacombe, ...
Previous challenge studies performed at Ohio State University involved a transport-stress model where the study animals were dosed with Sarcocystis neurona sporocysts on the day of arrival. This...
J. P. Dubey, M. J. Oglesbee, C. D. Sofaly, A. E. Marsh, E. Elitsur, ...
Sarcocystis neurona and Sarcocystis fayeri infections are common in horses in the Americas. Their antemortem diagnosis is important because the former causes a neurological disorder in horses,...
TOXOPLASMOSIS IN NILGAIS (BOSELAPHUS TRAGOCAMELUS) AND A SAIGA ANTELOPE (SAIGA TATARICA) (2004)
Kamil Sedlák, Eva Bártová, Ivan Literák, Roman Vodička, J. P. Dubey
Three captive female nilgais (Boselaphus tragocamelus) either showed late-term abortion or their newborn calves died within 2 days of birth. Toxoplasma gondii DNA was demonstrated in the brain and...
J. P. Dubey, M. Z. Levy, C. Sreekumar, S. K. Shen, E. Dahl, ...
The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii...
ANTIGENIC EVALUATION OF A RECOMBINANT BACULOVIRUS-EXPRESSED SARCOCYSTIS NEURONA SAG1 ANTIGEN (2004)
G. D. Gupta, J. Lakritz, W. J. Saville, R. S. Livingston, J. P. Dubey, J. R. Middleton, ...
Sarcocystis neurona is the primary parasite associated with equine protozoal myeloencephalitis (EPM). This is a commonly diagnosed neurological disorder in the Americas that infects the central...
SURVIVAL OF TOXOPLASMA GONDII OOCYSTS IN EASTERN OYSTERS (CRASSOSTREA VIRGINICA) (2004)
David S. Lindsay, Marina V. Collins, Sheila M. Mitchell, Carly N. Wetch, Alexa C. Rosypal, George J. Flick, ...
Toxoplasma gondii has recently been recognized to be widely prevalent in the marine environment. It has previously been determined that Eastern oysters (Crassostrea virginica) can remove sporulated...
J. P. Dubey, P. G. Parnell, C. Sreekumar, E. Dahl, ...
Toxoplasma gondii isolates can be grouped into 3 genetic lineages. Type I isolates are considered virulent to outbred mice, whereas Type II and III isolates are not. In the present report, viable T....
J. P. Dubey, C. Sreekumar, K. B. Miska, D. E. Hill, D. S. Lindsay
Neospora caninum and Hammondia heydorni are morphologically and phylogenetically related coccidians that are found in dogs. Although there is serological evidence of N. caninum infection in the...
J. P. Dubey, I. T. Navarro, C. Sreekumar, E. Dahl, R. L. Freire, H. H. Kawabata, ...
Cats are important in the epidemiology of Toxoplasma gondii because they are the only hosts that can excrete environmentally resistant oocysts. The prevalence of T. gondii was determined in 58...
First Isolation of Neospora caninum From an Aborted Bovine Fetus in Spain (2004)
N. Canada, C. S. Meireles, M. Mezo, M. González-Warleta, C. Sreekumar, ...
Neospora caninum was isolated from the brain of a 6-mo-old aborted bovine fetus from Galicia, Spain. The fetal brain homogenate was inoculated intraperitoneally into cortisonized mice. The peritoneal...
From a natural population that inhabits the dry evergreen forest at Polonnaruwa, serum samples of 170 toque macaques were examined for antibodies to Toxoplasma gondii by the modified agglutination...
Disseminated Toxoplasmosis in a Captive Ring-Tailed Lemur (Lemur catta) (2004)
J. A. Spencer, K. S. Joiner, C. D. Hilton, J. P. Dubey, M. Toivio-Kinnucan, J. K. Minc, ...
A 3-yr-old secundiparous female ring-tailed lemur presented to the Auburn University Small Animal Clinic with signs of dyspnea, lethargy, and anorexia. The animal died before she could be examined,...
Fatal Toxoplasmosis in a Bald Eagle (Haliaeetus leucocephalus) (2004)
K. A. Szabo, M. G. Mense, T. P. Lipscomb, K. J. Felix, J. P. Dubey
Toxoplasma gondii tachyzoites were identified in the myocardium of a bald eagle (Haliaeetus leucocephalus) that died of necrotizing myocarditis. The diagnosis was confirmed by immunohistochemical...
David S. Lindsay, Sheila M. Mitchell, M. C. Vianna, J. P. Dubey
Equine protozoal myeloencephalitis is a major cause of neurological disease in horses from the Americas. Horses are considered accidental intermediate hosts. The structure of sporocysts of the...
O. Cabezón, A. R. Resendes, M. Domingo, J. A Raga, C. Agustí, F. Alegre, ...
Although Toxoplasma gondii infection has been found occasionally in cetaceans, little is known of the prevalence of antibodies to T. gondii in wild dolphins. Antibodies to T. gondii were determined...
Mark Jenkins, Rodrigo Soares, Charles Murphy, Andrew Hemphill, Ryan O'Handley, J. P. Dubey
The purpose of the present study was to characterize the intracellular distribution of a native Neospora caninum 56-kDa protein that is recognized by sera from N. caninum–infected dairy cattle. The...
EXPERIMENTAL TOXOPLASMA GONDII INFECTION IN GREY SEALS (HALICHOERUS GRYPUS) (2004)
A. A. Gajadhar, L. Measures, L. B. Forbes, C. Kapel, J. P. Dubey
Laboratory-reared animals were used to assess the susceptibility of seals (Halichoerus grypus) to Toxoplasma gondii infection. Four seals were each orally inoculated with 100 or 10,000 oocysts of T....
Toxoplasmosis in an Elephant Seal (Mirounga angustirostris) (2004)
J. P. Dubey, T. P. Lipscomb, M. Mense
Toxoplasma gondii infections in fish-eating marine mammals is intriguing and indicative of contamination of the sea environment with oocysts. Toxoplasma gondii was identified in an elephant seal...
Isolation and Genotyping of Toxoplasma gondii From Free-Ranging Chickens From Mexico (2004)
J. P. Dubey, E. S. Morales, T. Lehmann
The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the presence of T. gondii oocysts in the environment because chickens feed from the soil. In the present study,...
J. P. Dubey, D. H. Graham, E. Dahl, M. L. Eberhard, E. K. Nace, ...
Toxoplasma gondii isolates can be grouped into 3 genetic lineages. Type I isolates are considered more virulent in outbred mice and have been isolated predominantly from clinical cases of human...
Intervet Symposium: bovine neosporosis (2004)
Schetters, T., Dubey, J.P., Adrianarivo, A., Frankena, K., Romero, J.J., Pérez, E., ...
D. Yantis, R. Moeller, R. Braun, C. H. Gardiner, A. Aguirre, J. P. Dubey
A Hawaiian monk seal (Monachus schauinslandi) died in captivity at the National Marine Fisheries Service, Kewalo Basin Facility in Honolulu, Hawaii. The animal was icteric, and the liver was friable....
Redescription of the Sarcocysts of Sarcocystis rileyi (Apicomplexa: Sarcocystidae) (2003)
J. P. DUBEY, R. J. CAWTHORN, C. A. SPEER, G. A. WOBESER
The intermediate hosts for Sarcocystis rileyi (Stiles 1893) Minchin 1913 are ducks (Anas spp.), and the striped skunk (Mephitis mephitis) is its definitive host. The structure of sarcocysts from an...
Sporulation and Survival of Toxoplasma gondii Oocysts in Seawater (2003)
DAVID. S. LINDSAY, MARINA V. COLLINS, SHEILA M. MITCHELL, REBECCA A. COLE, GEORGE J. FLICK, CARLY N. WETCH, ...
We have been collaborating since 1992 in studies on southern sea otters (Enhdyra lutris nereis) as part of a program to define factors, which may be responsible for limiting the growth of the...
Toxoplasma gondii Isolates From Free-Ranging Chickens From the United States (2003)
J. P. Dubey, D. H. Graham, E. Dahl, C. Sreekumar, T. Lehmann, M. F. Davis, ...
Prevalence of Toxoplasma gondii infection in chickens is a good indicator of the strains prevalent in their environment because they feed from ground. The prevalence of T. gondii was determined in...
Isolation and Genotyping of Toxoplasma gondii From Free-Ranging Chickens From Argentina (2003)
J. P. Dubey, M. C. Venturini, L. Venturini, M. Piscopo, D. H. Graham, E. Dahl, ...
The prevalence of Toxoplasma gondii in free-ranging chickens can be considered a good indicator of the prevalence of T. gondii oocysts in the environment because chickens feed from the ground. In the...
Seroprevalence of Toxoplasma gondii Antibodies in Domestic Cats from Barcelona, Spain (2003)
S. Almería, A. Ortuño, F. Garcia, J. P. Dubey
Cats are important in the epidemiology of Toxoplasma gondii infection because they are the only hosts that can excrete the environmentally resistant oocysts. Antibodies to T. gondii were determined...
C. Sreekumar, D. E. Hill, V. M. Fournet, B. M. Rosenthal, D. S. Lindsay, J. P. Dubey
Neospora caninum and Hammondia heydorni are morphologically and phylogenetically related coccidians that are found in dogs. New diagnostic genetic loci, based on random-amplified polymorphic...
J. P. Dubey, S. M. Mitchell, J. K. Morrow, J. C. Rhyan, L. M. Stewart, D. E. Granstrom, ...
Sarcocystis neurona, Neospora caninum, N. hughesi, and Toxoplasma gondii are 4 related coccidians considered to be associated with encephalomyelitis in horses. The source of infection for N. hughesi...
A. M. Joppert, C. E. Souza, J. P. Dubey, ...
Capybaras (Hidrochoeris hidrochoeris) are 1 of the largest rodents used for meat in South and Central America. Prevalence of anti–Toxoplasma gondii antibodies in 149 feral H. hidrochoeris from the...
J. P. Dubey, D. H. Graham, T. Lehmann
Most isolates of Toxoplasma gondii can be grouped into 3 genetic lineages. In the present study, 67 isolates of T. gondii were obtained by bioassay in mice inoculated with brains and hearts of 96...
J. M. Lapointe, P. J. Duignan, B. C. Barr, A. K. Petrich, D. W. MacPherson, F. M. Gulland, ...
A Pacific harbor seal (Phoca vitulina richardsii) was found on the central California coast with neurologic signs and labored breathing, which were unresponsive to treatment. Necropsy revealed a...
Autofluorescence of Toxoplasma gondii and Related Coccidian Oocysts (2003)
Jason W. Bennett, Jeff D. Hester, Michael W. Ware, J. P. Dubey, William V. Everson
This is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Neospora caninum, Besnoitia...
V. C. Geraldi, J. P. Dubey, ...
Antibodies to Neospora caninum and Toxoplasma gondii were assayed in sera of 396 opossums (Didelphis marsupialis) from the city of São Paulo, Brazil. Antibodies to N. caninum were assayed using the...
Ultrastructure of Besnoitia besnoiti Tissue Cysts and Bradyzoites (2003)
J. P. DUBEY, V. SHKAP, E. PIPANO, L. FISH, D. L. FRITZ
Besnoitia besnoiti is an economically important tissue cyst-forming apicomplexan of cattle in Africa and Israel. Tissue cysts and bradyzoites of B. besnoiti from the skin of a naturally infected bull...
Susan Liddell, Carolyn Parker, Bryan Vinyard, Mark Jenkins, J. P. Dubey
The purpose of the present study was to use direct plasmid deoxyribonucleic acid (DNA) injection to identify specific antigens that confer protection against congenital transfer of Neospora caninum....
S. K. Shen, Tovi Lehman, J. P. Dubey
The prevalence of Toxoplasma gondii in free-range chickens from Campos dos Goytacazes, Rio de Janeiro State, Brazil, was examined to evaluate environmental contamination by oocysts. Antibodies...
Sarcocysts of an Unidentified Species of Sarcocystis in the Sea Otter (Enhydra lutris) (2003)
J. P. Dubey, D. S. Lindsay, B. M. Rosenthal, N. J. Thomas
The number of Sarcocystis species that infect sea otters (Enhydra lutris) is unknown. Sea otter tissues were recently shown to harbor sarcocysts of S. neurona and of unidentified species of...
Dubey, J.P., Mitchell, S.M., Morrow, J.K., Rhyan, J.C., Stewart, L.M., Granstrom, D.E., ...
Sarcocystis neurona, Neospora caninum, N. hughesi, and Toxoplasma gondii are 4 related coccidians considered to be associated with encephalomyelitis in horses. The source of infection for N. hughesi...
Yantis, D., Moeller, R., Braun, R., Gardiner, C. H., Aguirre, A., Dubey, J. P.
A Hawaiian monk seal (Monachus schauinslandi) died in captivity at the National Marine Fisheries Service, Kewalo Basin Facility in Honolulu, Hawaii. The animal was icteric, and the liver was friable....
EXPERIMENTAL INFECTION OF SHEEP WITH NEOSPORA CANINUM OOCYSTS (2002)
Ryan O'Handley, Susan Liddell, Carolyn Parker, Mark C. Jenkins, J. P. Dubey
The purpose of the present study was to investigate the potential of Neospora caninum oocysts to infect sheep and determine whether N. caninum DNA could be detected by polymerase chain reaction (PCR)...
J. F. Stanek, J. P. Dubey, M. J. Oglesbee, S. M. Reed, D. S. Lindsay, L. A. Capitini, ...
Sarcocystis neurona causes encephalomyelitis in many species of mammals and is the most important cause of neurologic disease in the horse. Its complete life cycle is unknown, particularly its...
C. D. Sofaly, S. M. Reed, J. C. Gordon, J. P. Dubey, M. J. Oglesbee, C. J. Njoku, ...
The effect of inoculation dose of Sarcocystis neurona sporocysts on the development of clinical neurologic disease in horses was investigated. Twenty-four seronegative weanling horses were subjected...
J. P. Dubey, H. R. Gamble, D. Hill, C. Sreekumar, S. Romand, P. Thulliez
The ingestion of uncooked infected meat is considered important in the epidemiology of Toxoplasma gondii infection in humans and little is known of the prevalence of viable T. gondii in meat used for...
Isolation of Viable Toxoplasma gondii From Naturally Infected Aborted Bovine Fetuses (2002)
Nuno Canada, Carla S. Meireles, A. Rocha, M. W. Erickson, J. P. Dubey
Neospora caninum and Toxoplasma gondii are related parasites. The former is a common cause of abortion in dairy cattle. The latter has not been conclusively demonstrated in bovine fetuses. During the...
J. P. Dubey, D. S. Lindsay, D. Hill, S. Romand, P. Thulliez, ...
Antibodies to Neospora caninum and Sarcocystis neurona were determined in serum samples of 502 domestic cats from Brazil using direct agglutination tests with the respective antigens. Antibodies to...
J. P. Dubey, W. J. Saville, C. Sreekumar, S. K. Shen, D. S. Lindsay, H. F. Pena, ...
The effect of moist heat and several disinfectants on Sarcocystis neurona sporocysts was investigated. Sporocysts (4 million) were suspended in water and heated to 50, 55, 60, 65, and 70 C for...
A. R. Resendes, S. Almería, J. P. Dubey, E. Obón, C. Juan-Sallés, E. Degollada, ...
Fatal disseminated toxoplasmosis was diagnosed in a Risso's dolphin (Grampus griseus) dam and its fetus on the basis of pathologic findings, immunohistochemistry, and structure of the parasite. The...
Toxoplasmosis in Three Species of Native and Introduced Hawaiian Birds (2002)
Thierry M. Work, J. Gregory Massey, David S. Lindsay, J. P. Dubey
Toxoplasma gondii was found in endemic Hawaiian birds, including 2 nene geese (Nesochen sandvicensis), 1 red-footed booby (Sula sula), and an introduced bird, the Erckels francolin (Francolinus...
TACHYZOITE-INDUCED LIFE CYCLE OF TOXOPLASMA GONDII IN CATS (2002)
The tachyzoite-induced cycle of Toxoplasma gondii was studied in 46 cats. Tachyzoites of the M-7741 or Me-49 strain of T. gondii were administered orally to cats by pouring into the mouth or by...
Kalmia E. Kniel, David S. Lindsay, Susan S. Sumner, Cameron R. Hackney, Merle D. Pierson, J. P. Dubey
The consumption of Toxoplasma gondii oocysts on fresh produce may be a means of its transmission to humans. Cats shed T. gondii oocysts, which contaminate produce directly or contaminate water...
Prevalence of Toxoplasma gondii Antibodies in Domestic Cats From Rural Ohio (2002)
J. P. Dubey, J. F. Stanek, S. M. Reed
Antibodies to Toxoplasma gondii were determined in serum samples from 275 domestic cats from a mobile spay and neuter clinic from 8 counties in Ohio. The modified agglutination test incorporating...
Isolation of Purified Oocyst Walls and Sporocysts from Toxoplasma gondii (2002)
WILLIAM V. EVERSON, MICHAEL W. WARE, J. P. DUBEY
Toxoplasma gondii oocysts are environmentally resistant and can infect virtually all warm-blooded hosts, including humans and livestock. Little is known about the biochemical basis for this...
EXPERIMENTAL TOXOPLASMOSIS IN BUDGERIGARS (MELOPSITTACUS UNDULATUS) (2002)
The susceptibility of budgerigars (Melopsittacus undulatus) to graded doses of Toxoplasma gondii oocysts was studied. Sixteen budgerigars were divided into 4 groups (A–D) of 4 each. Birds in groups...
Joerg Kinne, Mansoor Ali, Ulrich Wernery, J. P. Dubey
Between January and March 2001, eight 4- to 8-wk-old camels (Camelus dromedarius) from 2 farms from Dubai area of the United Arab Emirates were submitted for necropsy examination. The camels had...
Intestinal Coccidiosis in a Spinner Dolphin (Stenella longirostris) (2002)
J. P. Dubey, J. S. Eggers, T. P. Lipscomb
Intestinal coccidiosis was diagnosed histologically in the small intestine of a spinner dolphin (Stenella longirostris). Numerous intralesional coccidia were present in mucosal epithelial cells....
Acute Visceral Toxoplasmosis in Captive Dik-Dik (Madoqua guentheri smithi) (2002)
J. P. Dubey, Maryanne E. Tocidlowski, Bruce Abbitt, S. Y. Llizo
Acute toxoplasmosis was diagnosed in 2 captive dik-dik (Madoqua guentheri smithi) in the Houston Zoo. Both animals became ill suddenly and died in spite of supportive therapy. Toxoplasma gondii was...
Prevalence of Neospora caninum Antibodies in Dogs From Dairy Cattle Farms in Parana, Brazil (2002)
J. S. Guimarães Jr., F. Ferreira, J. P. Dubey, S. M. Gennari
Serum samples from 134 dogs from 22 cattle dairy farms in the northern region of Parana State, Brazil, were tested for antibodies to Neospora caninum in an indirect fluorescent antibody test....
S. M. Gennari, V. R. Amajones, C. Magnabosco, ...
Antibodies to Toxoplasma gondii were determined in serum samples of 502 domestic cats from Brazil by the modified agglutination test (MAT), using formalin-fixed whole tachyzoites and mercaptoethanol....
J. P. Dubey, A. N. Hamir, M. J. Topper
Two structurally distinct types (A, B) of microscopic sarcocysts were found in muscles of 4 of 5 feral skunks. Type A sarcocysts had sarcocyst walls of up to 6 μm thick. The villar protrusions (Vp)...
Congenital Toxoplasmosis in a Indo-Pacific Bottlenose Dolphin (Tursiops aduncus) (2002)
Toxoplasma gondii was identified in tissues of a stillborn late-term fetus from an Indo-Pacific bottlenose dolphin (Tursiops aduncus). Myocardial necrosis and nonsuppurative inflammation in the heart...
A. R. Resendes, C. Juan-Sallés, S. Almeria, N. Majó, M. Domingo, J. P. Dubey
Fatal hepatic sarcocystosis was diagnosed in a striped dolphin (Stenella coeruleoalba) from the northeastern Spanish Mediterranean coast based on pathologic findings and the microscopic and...
J. P. Dubey, G. C. Johnson, A. Bermudez, K. W. Suedmeyer, D. L. Fritz
A Sarcocystis neurona-like parasite was associated with acute sarcocystosis in the brain of an ibis (Carphibis spinicollis). Numerous schizonts and merozoites were found extravascularly in...
STRUCTURE OF SARCOCYSTIS NEURONA SARCOCYSTS (2001)
J. P. Dubey, D. S. Lindsay, D. Fritz, C. A. Speer
The ultrastructure of Sarcocystis neurona sarcocysts was studied from muscle of an experimentally infected cat. The cat was killed 144 days after being fed sporocysts from a naturally infected...
J. P. Dubey, A. C. Rosypal, B. M. Rosenthal, N. J. Thomas, D. S. Lindsay, J. F. Stanek, ...
Although Sarcocystis neurona has been identified in an array of terrestrial vertebrates, recent recognition of its capacity to infect marine mammals was unexpected. Here, sarcocysts from 2 naturally...
CRYPTOSPORIDIUM CANIS N. SP. FROM DOMESTIC DOGS (2001)
R. Fayer, J. M. Trout, L. Xiao, U. M. Morgan, A. A. Lal, J. P. Dubey
Oocysts of Cryptosporidium, from the feces of a naturally infected dog and from an HIV-infected human, were identified as the previously reported canine genotype of Cryptosporidium parvum, hereafter...
J. P. Dubey, D. S. Lindsay, B. M. Rosenthal, C. E. Kerber, N. Kasai, ...
Isolates of Sarcocystis falcatula–like organisms from South American opossums were characterized based on biological and morphological criteria. Sporocysts from intestinal scrapings of 1 Didelphis...
High-Resolution Typing of Toxoplasma gondii Using Microsatellite Loci (2001)
C. R. Blackston, J. P. Dubey, E. Dotson, C. Su, P. Thulliez, D. Sibley, ...
High-resolution typing of Toxoplasma gondii is essential to understand the effect of genetic differences among strains on the variation in disease manifestation and transmission patterns. Current...
Early localization and parasitemia of Sarcocystis neurona were studied in gamma interferon gene knockout (KO) mice fed S. neurona sporocysts. Mice were examined for S. neurona infection...
J. P. Dubey, D. S. Lindsay, S. K. Shen
The dose-related infectivity of Sarcocystis neurona sporocysts and merozoites of 2 recent isolates of S. neurona was compared in gamma interferon knockout (KO) mice. Tenfold dilutions of sporocysts...
P. Labelle, J. P. Dubey, I. Mikaelian, N. Blanchette, R. Lafond, S. St-Onge, ...
The seroprevalence of antibodies to Toxoplasma gondii was investigated in trapped lynx (Lynx canadensis) and bobcats (Lynx rufus) from Québec, Canada. Forty-seven of 106 (44%) lynx and 4 of 10 (40%)...
D. M. Lambourn, S. J. Jeffries, J. P. Dubey
As part of the Puget Sound Ambient Monitoring Program of the Washington Department of Fish and Wildlife, serum samples from 380 harbor seals (Phoca vitulina) were tested for antibodies to Toxoplasma...
TOXOPLASMA GONDII ANTIBODIES IN EXOTIC WILD FELIDS FROM BRAZILIAN ZOOS (2001)
Serum samples from 37 captive exotic felids in 12 zoos from six Brazilian states were assayed for antibodies to Toxoplasma gondii by the modified agglutination test using formalin-fixed whole...
J. P. DUBEY, B. M. ROSENTHAL, C. A. SPEER
A new species, Sarcocystis lindsayi n. sp., is proposed for a parasite resembling Sarcocystis falcatula. It was obtained from the lungs and muscles of budgerigars (Melopsittacus undulatus) fed...
J. P. Dubey, M. M. Garner, M. D. Stetter, A. E. Marsh, B. C. Barr
An unidentified Sarcocystis falcatula-like infection was diagnosed in a captive bee-eater (Merops nubicus) in a zoo in Florida. The bird died suddenly, probably due to protozoa-associated pneumonia....
W. Basso, L. Venturini, M. C. Venturini, P. Moore, M. Rambeau, J. M. Unzaga, ...
Prevalence of anti-Neospora caninum antibodies was determined in sera of 320 dogs from Argentina using the indirect fluorescent antibody test (IFAT). Antibodies to N. caninum were found in 121 of 320...
First Isolation of Neospora caninum From the Feces of a Naturally Infected Dog (2001)
W. BASSO, L. VENTURINI, M. C. VENTURINI, D. E. HILL, S. K. SHEN, ...
Neospora caninum is a major cause of abortion in cattle worldwide. Cattle become infected with N. caninum by ingesting oocysts from the environment or transplacentally from dam to fetus....
CHARACTERIZATION OF THE OREGON ISOLATE OF NEOSPORA HUGHESI FROM A HORSE (2001)
J. P. Dubey, S. Liddell, D. Mattson, C. A. Speer, D. K. Howe, M. C. Jenkins
Neospora hughesi was isolated in cell cultures inoculated with homogenate of spinal cord from a horse in Oregon. Tachyzoites of this Oregon isolate of N. hughesi were maintained continuously by cell...
Dolores E. Hill, Susan Liddell, Mark C. Jenkins, J. P. Dubey
Neospora caninum oocysts, passed in the feces of a definitive host (dog), were isolated, and genomic DNA was extracted. A polymerase cahin reaction (PCR) targeting the N. caninum-specific Nc 5...
S. J. Kutz, B. T. Elkin, D. Panayi, J. P. Dubey
Prevalence of antibodies to Toxoplasma gondii was determined in 147 barren-ground caribou (Rangifer tarandus groenlandicus) from 5 herds in the Northwest Territories and Nunavut, northern Canada, by...
Infectivity of bradyzoites of the VEG strain of Toxoplasma gondii was compared in cats and mice. For this, tissue cysts were separated from brains of infected mice using a Percoll gradient, and...
J. P. Dubey, M. W. Garner, M. M. Willette, K. L. Batey, C. H. Gardiner
Fatal disseminated Toxoplasma gondii infection was diagnosed in 2 captive magpie geese (Anseranas semipalmata) from a zoo in Texas. Both geese died suddenly, without apparent clinical signs. Lesions...
Seroprevalence of Toxoplasma gondii in Elephants (Elephus maximus indicus) in Thailand (2001)
D. Tuntasuvan, K. Mohkaew, J. P. Dubey
Serum samples from captive 156 elephants (Elephus maximus indicus) from Thailand were examined for antibodies to Toxoplasma gondii using the modified agglutination test (MAT) and the latex...
COMPLETION OF THE LIFE CYCLE OF SARCOCYSTIS NEURONA (2000)
J. P. Dubey, D. S. Lindsay, R. W. Stich, J. F. Stanek, C. A. Speer, ...
Sarcocystis neurona is the most important cause of a neurologic disease in horses, equine protozoal myeloencephalitis (EPM). The complete life cycle of S. neurona, including the description of...
CLINICAL COCCIDIOSIS IN RACCOONS (PROCYON LOTOR) (2000)
J. P. Dubey, M. M. Garner, B. M. Rosenthal, D. DeGhetto
Clinical coccidiosis was diagnosed in wild-caught and captive raccoons. Eimeria procyonis-like oocysts were seen in 15 of 15 captive raccoons. In 6 of 6 juvenile wild-caught raccoons examined at...
J. P. DUBEY, D. S. LINDSAY, A. J. COSTA
An unidentified isolate of a Sarcocystis falcatula-like parasite was obtained from the lungs of budgerigars (Melopsittacus undulatus) fed sporocysts from a naturally-infected South American opossum,...
Radio-telemetry and geographical information systems to assess urban deer zoonoses (2000)
Hollis, Karmen M., Anchor, Christopher L., Chelsvig, James E., Etter, Dwayne R., Dubey, J. P., Warner, Richard E., ...
Urban white-tailed deer (Odocoileus virginianus) populations can influence the epidemiology of many zoonotic diseases because they affect the distribution and abundance of pathogens and vectors. The...
Frenkelia microti Infection in a Chinchilla (Chinchilla laniger) in the United States (2000)
J. P. Dubey, T. R. Clark, D. Yantis
A Frenkelia microti tissue cyst was found in the brain of a chinchilla (Chinchilla laniger) used for biomedical research. This is the first report of Frenkelia infection in this animal in the United...
In the central nervous system of 2 raccoons, 1 cat, 1 pony, 2 mink, and 1 skunk, protozoa previously thought to be Sarcocystis-like reacted positively to Sarcocystis neurona-specific antibodies in an...
David S. Lindsay, Richard D. McKown, J. P. Dubey
Grossly visible sarcocysts were seen in the skeletal muscles of 1 of 12 13-lined ground squirrels, Spermophilus tridecemlineatus tridecemlineatus, collected in Nebraska. The tissue cyst wall was up...
STRAIN TYPING OF TOXOPLASMA GONDII: COMPARISON OF ANTIGEN-CODING AND HOUSEKEEPING GENES (2000)
T. Lehmann, C. R. Blackston, S. F. Parmley, J. S. Remington, J. P. Dubey
Molecular characterization of Toxoplasma gondii isolates is central for understanding differences in disease transmission and manifestations. Only 3 subgroups (lineages) have been discerned with...
IN VITRO CULTIVATION OF SCHIZONTS OF SARCOCYSTIS SPEERI Dubey and Lindsay, 1999 (2000)
J. P. Dubey, C. A. Speer, D. S. Lindsay
Schizonts of Sarcocystis speeri Dubey and Lindsay, 1999 were cultured in vitro in bovine monocyte and equine kidney cell cultures inoculated with infected tissues of nude and γ-interferon knockout...
PREVALENCE OF SARCOCYSTIS SPECIES SPOROCYSTS IN WILD-CAUGHT OPOSSUMS (DIDELPHIS VIRGINIANA) (2000)
Sarcocystis sporocysts were found in intestinal scrapings from 24 (54.5%) of 44 opossums (Didelphis virginiana). The number of sporocysts varied from a few (
S. J. Kutz, B. Elkin, A. Gunn, J. P. Dubey
Prevalence of antibodies to Toxoplasma gondii was determined in 203 muskoxen (Ovibos moschatus) from 3 geographically distinct areas of northern Canada (near the hamlets of Kugluktuk and Cambridge...
USE OF RECOMBINANT ANTIGENS FOR DETECTION OF TOXOPLASMA GONDII INFECTION IN SWINE (2000)
H. Ray Gamble, Carolyn D. Andrews, J. P. Dubey, Donald W. Webert, Stephen F. Parmley
Five recombinant Toxoplasma gondii antigens, designated B427, C51, C55, V22, and MBP30 were assessed for their potential use in an enzyme-linked immunoassay (EIA) for detection of T. gondii infection...
R. A. Cole, D. S. Lindsay, D. K. Howe, C. L. Roderick, J. P. Dubey, N. J. Thomas, ...
Toxoplasma gondii was isolated from brain or heart tissue from 15 southern sea otters (Enhydra lutris nereis) in cell cultures. These strains were used to infect mice that developed antibodies to T....
Seroprevalence of Toxoplasma gondii in Rocky Mountain Bighorn Sheep (Ovis canadensis) (2000)
Serum samples from 697 Rocky Mountain bighorn sheep (Ovis canadensis) from North America were examined for antibodies to Toxoplasma gondii by the modified agglutination test incorporating...
Prevalence of Antibodies to Toxoplasma gondii in Ostriches (Struthio camelus) (2000)
J. P. Dubey, W. B. Scandrett, A. A. Gajadhar
Serum samples from 973 ostriches (Struthio camelus) in Canada were examined for antibodies to Toxoplasma gondii by the modified agglutination test incorporating mercaptoethanol and formalin-fixed...
J. P. Dubey, C. A. Speer, D. D. Bowman, K. M. Horton, C. Venturini, L. Venturini
Sarcocystis speeri Dubey and Lindsay, 1999 from the South American opossum Didelphis albiventris was successfully transmitted to the North American opossum Didelphis virginiana. Sporocysts from a...
Prevalence of Toxoplasma gondii Antibodies in Sera of Turkeys, Chickens, and Ducks from Egypt (2000)
A. El-Massry, O. A. Mahdy, A. El-Ghaysh, J. P. Dubey
Sera from 173 turkeys, 108 chickens, and 48 ducks from Giza, Egypt, were tested for the presence of anti-Toxoplasma gondii antibodies by means of the modified agglutination test using mercaptoethanol...
TOXOPLASMOSIS AS A SUSPECTED CAUSE OF ABORTION IN A GREENLAND MUSKOX (OVIBOS MOSHATUS WARDI) (2000)
Graham C. Crawford, Freeland H. Dunker, J. P. Dubey
Toxoplasma gondii tachyzoites were seen in the placenta of a late-term aborted Greenland muskox (Ovibos moschatus wardi) fetus in a captive herd at the San Francisco Zoo. The organism stained with...
J. P. Dubey, D. S. Lindsay, L. Venturini, C. Venturini
Two isolates of Sarcocystis falcatula were obtained from the lungs of budgerigars (Melopsittacus undulatus) fed sporocysts from two naturally-infected South American opossums (Didelphis albiventris)....
J. P. Dubey, L. Venturini, M. C. Venturini, C. A. Speer
Sarcocystis sporocysts from the intestines of 2 opossums (Didelphis albiventris) from Argentina were fed to gamma-interferon knockout (KO) and nude mice. Protozoal schizonts were seen in brain,...
Diclazuril is a benzeneacetonitril anticoccidial that has excellent activity against the extraintestinal stages of Toxoplasma gondii and Neospora caninum. It also is highly active against intestinal...
C. A. Speer, J. P. Dubey, D. E. Mattson
The development and merozoite production of Sarcocystis falcatula and 2 isolates (SN6 and SN2) of Sarcocystis neurona were studied in various cultured cell lines inoculated with culture-derived...
Development of a Real-Time PCR Assay for Detection of Toxoplasma gondii in Pig and Mouse Tissues
Jauregui, L. H., Higgins, J., Zarlenga, D., Dubey, J. P., Lunney, J. K.
A highly sensitive and specific method has been developed to reproducibly detect and quantitate Toxoplasma gondii burden in animal tissue samples using T. gondii ITS1-derived primers and a...
Detection of Toxoplasma gondii Oocysts in Drinking Water
Isaac-Renton, Judith, Bowie, William R., King, Arlene, Irwin, G. Stewart, Ong, Corinne S., Fung, C. P., ...
The world’s largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of...
Dubey, J. P., Lindsay, D. S., Speer, C. A.
Infections by the protozoan parasite Toxoplasma gondii are widely prevalent worldwide in animals and humans. This paper reviews the life cycle; the structure of tachyzoites, bradyzoites, oocysts,...
Howe, Daniel K., Tang, Keliang, Conrad, Patricia A., Sverlow, Karen, Dubey, J. P., Sibley, L. David
Neosporosis is an economically important disease of dairy cattle caused by the protozoan Neospora caninum. Diagnostic tests for neosporosis are complicated by the potential for cross-reaction of...
Identification of quantitative trait loci controlling acute virulence in Toxoplasma gondii
Su, Chunlei, Howe, Daniel K., Dubey, J. P., Ajioka, James W., Sibley, L. David
Strains of Toxoplasma gondii can be grouped into three predominant clonal lineages with members of the type I group being uniformly lethal in mice. To elucidate the basis of this extreme virulence, a...
Lally, N C, Jenkins, M C, Dubey, J P
Neospora caninum is a recently described apicomplexan parasite which causes paralysis and death in dogs. Neospora parasites also cause abortion and neonatal morbidity in cattle, sheep, goats, and...
Jenkins, M C, Wouda, W, Dubey, J P
Recombinant Neospora caninum tachyzoite antigens were evaluated in an enzyme-linked immunosorbent assay (ELISA) for recognition by serum antibodies (Ab) from Neospora-infected cattle. Serum samples...
Biology of Isospora spp. from humans, nonhuman primates, and domestic animals.
Lindsay, D S, Dubey, J P, Blagburn, B L
Coccidial parasites of the genus Isospora cause intestinal disease in several mammalian host species. These protozoal parasites have asexual and sexual stages within intestinal cells of their hosts...
Ho, M S, Barr, B C, Marsh, A E, Anderson, M L, Rowe, J D, Tarantal, A F, ...
Neospora is a newly recognized genus of pathogenic coccidia, closely related to Toxoplasma gondii, that can cause abortion or congenital disease in a variety of domestic animal hosts. On the basis of...
Baszler, T V, Knowles, D P, Dubey, J P, Gay, J M, Mathison, B A, McElwain, T F
Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii, causes abortion and congenital infection in cattle. To investigate specific methods of antemortem diagnosis, the antibody...
Rat model of congenital toxoplasmosis.
A rat model of congenital toxoplasmosis is described for the study of protective immunity and chemotherapy during pregnancy. Six Sprague-Dawley 7- to 15-day gestational rats were inoculated orally...
Lindsay, D S, Blagburn, B L, Dubey, J P
Neospora caninum and Toxoplasma gondii are structurally related protozoal parasites of mammals that may cause abortion and neonatal morbidity and mortality. Groups of mice were subcutaneously...
Hughes, H P, Speer, C A, Kyle, J E, Dubey, J P
Macrophage (M phi)-activating lymphokines present in concanavalin A-stimulated bovine T-lymphocyte cultures (ConAS) were studied by assessing their effects on Eimeria bovis and Toxoplasma gondii...
Jenkins, Mark, Parker, Carolyn, Tuo, Wenbin, Vinyard, Bryan, Dubey, J. P.
The present study showed that incorporation of CpG adjuvant into plasmid DNA coding for NcGRA7 antigen resulted in a twofold increase in the level of protection against congenital transfer of...
Bjerkas, I, Jenkins, M C, Dubey, J P
The purpose of the present study was to identify antigens of the protozoan Neospora caninum that could be useful for the diagnosis of neosporosis in domestic animals. As revealed by immunoblotting,...
Cross-immunity between Hammondia and Toxoplasma infections in mice and hamsters.
Cross-immunity between six strains of Hammondia hammondi and the M-7741 strain of Toxoplasma gondii was studied in mice and hamsters. Mice and hamsters were inoculated orally with 10(5) H. hammondi...
Dawson, Harry D., Beshah, Ethiopia, Nishi, Sandra, Solano-Aguilar, Gloria, Morimoto, Motoko, Zhao, Aiping, ...
Human infectious diseases have been studied in pigs because the two species have common microbial, parasitic, and zoonotic organisms, but there has been no systematic evaluation of cytokine gene...
Khan, Asis, Taylor, Sonya, Su, Chunlei, Mackey, Aaron J., Boyle, Jon, Cole, Robert, ...
Toxoplasma gondii is a highly successful protozoan parasite in the phylum Apicomplexa, which contains numerous animal and human pathogens. T.gondii is amenable to cellular, biochemical, molecular and...
Tuo, Wenbin, Fetterer, Raymond, Jenkins, Mark, Dubey, J. P.
Gamma interferon (IFN-γ) response is essential to the development of a host protective immunity in response to infections by intracellular parasites. Neosporosis, an infection caused by the...
Gliding Motility Leads to Active Cellular Invasion by Cryptosporidium parvum Sporozoites
Wetzel, Dawn M., Schmidt, Joann, Kuhlenschmidt, Mark S., Dubey, J. P., Sibley, L. David
We examined gliding motility and cell invasion by an early-branching apicomplexan, Cryptosporidium parvum, which causes diarrheal disease in humans and animals. Real-time video microscopy...
Hoane, Jessica S., Morrow, Jennifer K., Saville, William J., Dubey, J. P., Granstrom, David E., Howe, Daniel K.
Sarcocystis neurona is the primary causative agent of equine protozoal myeloencephalitis (EPM), a common neurologic disease of horses in the Americas. We have developed a set of enzyme-linked...
Development of a Real-Time PCR Assay for Detection of Toxoplasma gondii in Pig and Mouse Tissues
Jauregui, L. H., Higgins, J., Zarlenga, D., Dubey, J. P., Lunney, J. K.
A highly sensitive and specific method has been developed to reproducibly detect and quantitate Toxoplasma gondii burden in animal tissue samples using T. gondii ITS1-derived primers and a...
Detection of Toxoplasma gondii Oocysts in Drinking Water
Isaac-Renton, Judith, Bowie, William R., King, Arlene, Irwin, G. Stewart, Ong, Corinne S., Fung, C. P., ...
The world’s largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of...
Dubey, J. P., Lindsay, D. S., Speer, C. A.
Infections by the protozoan parasite Toxoplasma gondii are widely prevalent worldwide in animals and humans. This paper reviews the life cycle; the structure of tachyzoites, bradyzoites, oocysts,...
Howe, Daniel K., Tang, Keliang, Conrad, Patricia A., Sverlow, Karen, Dubey, J. P., Sibley, L. David
Neosporosis is an economically important disease of dairy cattle caused by the protozoan Neospora caninum. Diagnostic tests for neosporosis are complicated by the potential for cross-reaction of...
Identification of quantitative trait loci controlling acute virulence in Toxoplasma gondii
Su, Chunlei, Howe, Daniel K., Dubey, J. P., Ajioka, James W., Sibley, L. David
Strains of Toxoplasma gondii can be grouped into three predominant clonal lineages with members of the type I group being uniformly lethal in mice. To elucidate the basis of this extreme virulence, a...
Lally, N C, Jenkins, M C, Dubey, J P
Neospora caninum is a recently described apicomplexan parasite which causes paralysis and death in dogs. Neospora parasites also cause abortion and neonatal morbidity in cattle, sheep, goats, and...
Jenkins, M C, Wouda, W, Dubey, J P
Recombinant Neospora caninum tachyzoite antigens were evaluated in an enzyme-linked immunosorbent assay (ELISA) for recognition by serum antibodies (Ab) from Neospora-infected cattle. Serum samples...
Biology of Isospora spp. from humans, nonhuman primates, and domestic animals.
Lindsay, D S, Dubey, J P, Blagburn, B L
Coccidial parasites of the genus Isospora cause intestinal disease in several mammalian host species. These protozoal parasites have asexual and sexual stages within intestinal cells of their hosts...
Ho, M S, Barr, B C, Marsh, A E, Anderson, M L, Rowe, J D, Tarantal, A F, ...
Neospora is a newly recognized genus of pathogenic coccidia, closely related to Toxoplasma gondii, that can cause abortion or congenital disease in a variety of domestic animal hosts. On the basis of...
Baszler, T V, Knowles, D P, Dubey, J P, Gay, J M, Mathison, B A, McElwain, T F
Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii, causes abortion and congenital infection in cattle. To investigate specific methods of antemortem diagnosis, the antibody...
Rat model of congenital toxoplasmosis.
A rat model of congenital toxoplasmosis is described for the study of protective immunity and chemotherapy during pregnancy. Six Sprague-Dawley 7- to 15-day gestational rats were inoculated orally...
Lindsay, D S, Blagburn, B L, Dubey, J P
Neospora caninum and Toxoplasma gondii are structurally related protozoal parasites of mammals that may cause abortion and neonatal morbidity and mortality. Groups of mice were subcutaneously...
Hughes, H P, Speer, C A, Kyle, J E, Dubey, J P
Macrophage (M phi)-activating lymphokines present in concanavalin A-stimulated bovine T-lymphocyte cultures (ConAS) were studied by assessing their effects on Eimeria bovis and Toxoplasma gondii...
Jenkins, Mark, Parker, Carolyn, Tuo, Wenbin, Vinyard, Bryan, Dubey, J. P.
The present study showed that incorporation of CpG adjuvant into plasmid DNA coding for NcGRA7 antigen resulted in a twofold increase in the level of protection against congenital transfer of...
Bjerkas, I, Jenkins, M C, Dubey, J P
The purpose of the present study was to identify antigens of the protozoan Neospora caninum that could be useful for the diagnosis of neosporosis in domestic animals. As revealed by immunoblotting,...
Cross-immunity between Hammondia and Toxoplasma infections in mice and hamsters.
Cross-immunity between six strains of Hammondia hammondi and the M-7741 strain of Toxoplasma gondii was studied in mice and hamsters. Mice and hamsters were inoculated orally with 10(5) H. hammondi...
Dawson, Harry D., Beshah, Ethiopia, Nishi, Sandra, Solano-Aguilar, Gloria, Morimoto, Motoko, Zhao, Aiping, ...
Human infectious diseases have been studied in pigs because the two species have common microbial, parasitic, and zoonotic organisms, but there has been no systematic evaluation of cytokine gene...
Khan, Asis, Taylor, Sonya, Su, Chunlei, Mackey, Aaron J., Boyle, Jon, Cole, Robert, ...
Toxoplasma gondii is a highly successful protozoan parasite in the phylum Apicomplexa, which contains numerous animal and human pathogens. T.gondii is amenable to cellular, biochemical, molecular and...
Tuo, Wenbin, Fetterer, Raymond, Jenkins, Mark, Dubey, J. P.
Gamma interferon (IFN-γ) response is essential to the development of a host protective immunity in response to infections by intracellular parasites. Neosporosis, an infection caused by the...
Gliding Motility Leads to Active Cellular Invasion by Cryptosporidium parvum Sporozoites
Wetzel, Dawn M., Schmidt, Joann, Kuhlenschmidt, Mark S., Dubey, J. P., Sibley, L. David
We examined gliding motility and cell invasion by an early-branching apicomplexan, Cryptosporidium parvum, which causes diarrheal disease in humans and animals. Real-time video microscopy...
Hoane, Jessica S., Morrow, Jennifer K., Saville, William J., Dubey, J. P., Granstrom, David E., Howe, Daniel K.
Sarcocystis neurona is the primary causative agent of equine protozoal myeloencephalitis (EPM), a common neurologic disease of horses in the Americas. We have developed a set of enzyme-linked...
Globalization and the population structure of Toxoplasma gondii
Lehmann, Tovi, Marcet, Paula L., Graham, Doug H., Dahl, Erica R., Dubey, J. P.
Toxoplasma gondii is a protozoan parasite that infects nearly all mammal and bird species worldwide. Usually asymptomatic, toxoplasmosis can be severe and even fatal to many hosts, including people....
Hughes, H P, Kasper, L H, Little, J, Dubey, J P
The active phase of primary and challenge oral infections of Toxoplasma gondii was investigated with respect to natural killer (NK) activity against YAC-1 tumour cell targets in vitro and serum...
Novel Triazine JPC-2067-B Inhibits Toxoplasma gondii In Vitro and In Vivo
Mui, Ernest J., Schiehser, Guy A., Milhous, Wilbur K., Hsu, Honghue, Roberts, Craig W., Kirisits, Michael, ...
Toxoplasmosis is a neglected tropical disease, an emerging disease as well as a significant problem in developed countries causing a substantial health burden. Better medicines with less toxicity are...
Recent transcontinental sweep of Toxoplasma gondii driven by a single monomorphic chromosome
Khan, A., Fux, B., Su, C., Dubey, J. P., Darde, M. L., Ajioka, J. W., ...
Toxoplasma gondii is a highly prevalent protozoan parasite that infects a wide range of animals and threatens human health by contaminating food and water. A markedly limited number of clonal...
Epidemiology and Control of Neosporosis and Neospora caninum
Dubey, J. P., Schares, G., Ortega-Mora, L. M.
Neospora caninum is a protozoan parasite of animals. Until 1988, it was misidentified as Toxoplasma gondii. Since its first recognition in dogs in 1984 and the description of the new genus and...
THE TOXOPLASMA GONDII OOCYST FROM CAT FECES
Dubey, J. P., Miller, Nancy L., Frenkel, J. K.
Coccidian oocysts resembling those of Isospora bigemina were excreted by cats fed Toxoplasma. In order to identify these oocysts with Toxoplasma infectivity a number of critical comparisons were...
Dogs as Sources and Sentinels of Parasites in Humans and Wildlife, Northern Canada
Salb, Amanda L., Barkema, Herman W., Elkin, Brett T., Thompson, R.C. Andrew, Whiteside, Douglas P., Black, Sandra R., ...
A minimum of 11 genera of parasites, including 7 known or suspected to cause zoonoses, were detected in dogs in 2 northern Canadian communities. Dogs in remote settlements receive minimal veterinary...
Review of Neospora caninum and neosporosis in animals
Neospora caninum is a coccidian parasite of animals. It is a major pathogen for cattle and dogs and it occasionally causes clinical infections in horses, goats, sheep, and deer. Domestic dogs are the...
Seroprevalence of antibodies to Neospora caninum in dogs and raccoon dogs in Korea
Kim, Jae-Hoon, Kang, Min-Soo, Lee, Byung-Chun, Hwang, Woo-Suk, Lee, Chang-Woo, So, Byung-Jae, ...
Neospora caninum is an important cause of abortion in cattle, and dogs are its only known definitive host. Its seroprevalence among domestic urban and rural dogs and feral raccoon dogs (Nyctereutes...
Novel triazine JPC-2067-B inhibits Toxoplasma gondii in vitro and in vivo
Mui, Ernest J., Schiehser, Guy A., Milhous, Wilbur K., Hsu, Honghue, Roberts, C.W., Kirisits, Michael J., ...
Toxoplasma gondii causes substantial morbidity, mortality, and costs for healthcare in the developed and developing world. Current medicines are not well tolerated and cause hypersensitivity...
Potential contamination of drinking water with Toxoplasma gondii oocysts.
Aramini, J. J., Stephen, C., Dubey, J. P., Engelstoft, C., Schwantje, H., Ribble, C. S.
The world's first documented toxoplasmosis outbreak associated with a municipal water supply was recognized in 1995 in Victoria, British Columbia, Canada. It was hypothesized that domestic cat (Felis...