We have previously shown that estradiol treatment of roosters resulted in a rapid loss of binding activity of the repressor MDBP-2-H1 (a member of the histone H1 family) to methylated DNA that was...
Here I show that nuclear extracts of chicken embryos can promote the active demethylation of DNA. The evidence shows that in hemimethylated DNA (i.e., methylated on one strand only) demethylation of...
MDBP-2 is a repressor that binds preferentially to methylated DNA. Peptides derived from MDBP-2 were sequenced. The sequences of the two peptides, KPAGPS-VTELITK and ALAAGGYDVEK, are identical to...
Vaccaro, M, Pawlak, A, Jost, J P
A homologous in vitro transcription system was developed in which the cloned chicken vitellogenin II gene is faithfully transcribed by extracts prepared from chicken liver nuclei. The use of template...
We have previously shown that in vivo estradiol-dependent dephosphorylation of MDBP-2-H1 (a member of the histone H1 family) correlates with the loss of in vitro preferential binding to methylated...
Upon the onset of mouse myoblast differentiation there is a rapid drop in DNA methyltransferase activity followed by a genome wide demethylation [Jost and Jost (1994) J. Biol. Chem. 269,...
Frémont, M, Siegmann, M, Gaulis, S, Matthies, R, Hess, D, Jost, J P
We have previously purified and characterized a 5-methylcytosine (5-MeC)-DNA glycosylase from 12 day old chick embryos [Jost,J.P. et al. (1995) J. Biol. Chem. 270, 9734-9739]. The activity of the...
The RNA moiety of chick embryo 5-methylcytosine- DNA glycosylase targets DNA demethylation.
Jost, J P, Frémont, M, Siegmann, M, Hofsteenge, J
We have previously shown that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. RNA from enzyme purified by SDS-PAGE was isolated and cloned. The...
Schwarz, S, Hess, D, Jost, J P
The methylated DNA binding protein-2-H1 (MDBP-2-H1), present in rooster liver, is a member of the histone H1 family which inhibits transcription by binding selectively to methylated promoters. Here...
Liu, Y, Oakeley, E J, Sun, L, Jost, J P
It has been shown that, during the S-phase of the cell cycle, the mouse DNA methyltransferase (DNA MTase) is targeted to sites of DNA replication by an amino acid sequence (aa 207-455) lying in the...
Jost, J P, Schwarz, S, Hess, D, Angliker, H, Fuller-Pace, F V, Stahl, H, ...
We have shown previously that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. Amino acid sequences of nine peptides derived from a highly...
Saluz, H P, Feavers, I M, Jiricny, J, Jost, J P
Genomic sequencing was used to study the in vivo methylation pattern of two CpG sites in the promoter region of the avian vitellogenin gene. The CpG at position +10 was fully methylated in DNA...
We describe a method that permits the study of the state of cytosine methylation and of in vivo protein-DNA interactions in higher eukaryotes. This powerful technique is applicable to any gene of...
Feavers, I M, Jiricny, J, Moncharmont, B, Saluz, H P, Jost, J P
The DNA sequence corresponding to the estradiol response element has been synthesized and tested in vitro for the binding of specific proteins. Gel retardation experiments combined with dimethyl...
Wilks, A, Seldran, M, Jost, J P
A HpaII site 611 base pairs upstream from the 5' end of the chicken vitellogenin II gene exhibits an estrogen-dependent demethylation in hormone-responsive cells. The complete demethylation of this...
Jost, J P, Moncharmont, B, Jiricny, J, Saluz, H, Hertner, T
The vitellogenin II gene is specifically reactivated in vitro (secondary stimulation, memory effect) in purified liver nuclei that had ceased to express the gene in vivo a month after the roosters...
Jost, J P, Saluz, H P, Pawlak, A
A negative regulating protein (MDBP-2) from rooster liver nuclear extracts binds preferentially to a methylated promoter region 5'TTCACCTTmCGCTATGAGGGGGATCATACTGG3' of the avian vitellogenin II gene...
Zingg, J M, Alva, G P, Jost, J P
We have isolated the mouse MyoD1 gene flanked by its promoter region by screening a genomic library with synthetic oligonucleotides. The structural gene is interrupted by two G + C rich introns....
An estrogen-dependent protein present in chicken liver polysomes binds to the 5' untranslated region of the chicken vitellogenin II mRNA. Competition binding assays with different RNAs indicate that...
Pawlak, A, Bryans, M, Jost, J P
In vitro transcription competition with oligonucleotides has shown that a down regulating factor can be displaced by a methylated oligonucleotide covering a specific region of the avian vitellogenin...
In gel shift assays, affinity chromatography-purified NHP1 forms a stable complex with different sequences of the chicken vitellogenin II gene. The apparent KD of NHP1 with the estrogen response...
In vitro RNA synthesis and expression of vitellogenin gene in isolated chicken liver nuclei.
Optimal conditions for prolonged in vitro synthesis of RNA in isolated chicken liver nuclei have been described. It is shown by incorporation of gamma32P-GTP into RNA, analysis of the product on...
Identification of a large precursor of vitellogenin mRNA in the liver of estradiol-treated chicks.
Jost, J P, Pehling, G, Ohno, T, Cozens, P
Studies were performed to determine whether vitellogenin mRNA from avian liver has a precursor molecule or not. Total cellular RNA was prepared from estradiol-treated chicken liver in the presence of...
Jost, J P, Seldran, M, Geiser, M
DNA-cellulose competition binding assays were used to measure the ability of cloned DNA fragments of the chicken vitellogenin II gene to displace the estrogen-receptor complex from total chicken DNA...
Estrogen induces a demethylation at the 5' end region of the chicken vitellogenin gene.
Wilks, A F, Cozens, P J, Mattaj, I W, Jost, J P
The two isoschizomeric restriction endonucleases Msp I and Hpa II have been used to study changes in the methylation pattern of the vitellogenin gene after estrogen treatment. In the liver of the...
Saluz, H P, Jiricny, J, Jost, J P
Genomic sequencing was used to study the extent of cytosine methylation of four CpG sites within the regulatory region of the estradiol-inducible avian vitellogenin II gene. Three of these sites, two...
Jost, J P, Geiser, M, Seldran, M
Avian vitellogenin-cauliflower mosaic virus hybrid gene is effectively transcribed in vitro in the homologous embryonic liver nuclei system. The transcription of the hybrid gene is modulated by the...
MyoD1 promoter autoregulation is mediated by two proximal E-boxes.
Zingg, J M, Pedraza-Alva, G, Jost, J P
We show that in mouse myoblasts the MyoD1 promoter is highly stimulated by MyoD1 expression, suggesting that it is controlled by a positive feedback loop. Using deletion and mutation analyses, we...
Supercoiled DNA loops linked to the nuclear matrix can be progressively cleaved with deoxyribonuclease I. The DNA which remains associated with the nuclear matrix can be purified and analysed for...
DNA binding and methyl transfer catalysed by mouse DNA methyltransferase.
Reale, A, Lindsay, H, Saluz, H P, Pradhan, S, Adams, R L, Jost, J P, ...
By using a purified fraction of mouse DNA methyltransferase we have shown, by gel-retardation analysis, that the enzyme forms a low-affinity complex preferentially with hemimethylated DNA; the...
We have previously shown that estradiol treatment of roosters resulted in a rapid loss of binding activity of the repressor MDBP-2-H1 (a member of the histone H1 family) to methylated DNA that was...
Here I show that nuclear extracts of chicken embryos can promote the active demethylation of DNA. The evidence shows that in hemimethylated DNA (i.e., methylated on one strand only) demethylation of...
MDBP-2 is a repressor that binds preferentially to methylated DNA. Peptides derived from MDBP-2 were sequenced. The sequences of the two peptides, KPAGPS-VTELITK and ALAAGGYDVEK, are identical to...
Vaccaro, M, Pawlak, A, Jost, J P
A homologous in vitro transcription system was developed in which the cloned chicken vitellogenin II gene is faithfully transcribed by extracts prepared from chicken liver nuclei. The use of template...
We have previously shown that in vivo estradiol-dependent dephosphorylation of MDBP-2-H1 (a member of the histone H1 family) correlates with the loss of in vitro preferential binding to methylated...
Upon the onset of mouse myoblast differentiation there is a rapid drop in DNA methyltransferase activity followed by a genome wide demethylation [Jost and Jost (1994) J. Biol. Chem. 269,...
Frémont, M, Siegmann, M, Gaulis, S, Matthies, R, Hess, D, Jost, J P
We have previously purified and characterized a 5-methylcytosine (5-MeC)-DNA glycosylase from 12 day old chick embryos [Jost,J.P. et al. (1995) J. Biol. Chem. 270, 9734-9739]. The activity of the...
The RNA moiety of chick embryo 5-methylcytosine- DNA glycosylase targets DNA demethylation.
Jost, J P, Frémont, M, Siegmann, M, Hofsteenge, J
We have previously shown that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. RNA from enzyme purified by SDS-PAGE was isolated and cloned. The...
Schwarz, S, Hess, D, Jost, J P
The methylated DNA binding protein-2-H1 (MDBP-2-H1), present in rooster liver, is a member of the histone H1 family which inhibits transcription by binding selectively to methylated promoters. Here...
Liu, Y, Oakeley, E J, Sun, L, Jost, J P
It has been shown that, during the S-phase of the cell cycle, the mouse DNA methyltransferase (DNA MTase) is targeted to sites of DNA replication by an amino acid sequence (aa 207-455) lying in the...
Jost, J P, Schwarz, S, Hess, D, Angliker, H, Fuller-Pace, F V, Stahl, H, ...
We have shown previously that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. Amino acid sequences of nine peptides derived from a highly...
Saluz, H P, Feavers, I M, Jiricny, J, Jost, J P
Genomic sequencing was used to study the in vivo methylation pattern of two CpG sites in the promoter region of the avian vitellogenin gene. The CpG at position +10 was fully methylated in DNA...
We describe a method that permits the study of the state of cytosine methylation and of in vivo protein-DNA interactions in higher eukaryotes. This powerful technique is applicable to any gene of...
Feavers, I M, Jiricny, J, Moncharmont, B, Saluz, H P, Jost, J P
The DNA sequence corresponding to the estradiol response element has been synthesized and tested in vitro for the binding of specific proteins. Gel retardation experiments combined with dimethyl...
Wilks, A, Seldran, M, Jost, J P
A HpaII site 611 base pairs upstream from the 5' end of the chicken vitellogenin II gene exhibits an estrogen-dependent demethylation in hormone-responsive cells. The complete demethylation of this...
Jost, J P, Moncharmont, B, Jiricny, J, Saluz, H, Hertner, T
The vitellogenin II gene is specifically reactivated in vitro (secondary stimulation, memory effect) in purified liver nuclei that had ceased to express the gene in vivo a month after the roosters...
Jost, J P, Saluz, H P, Pawlak, A
A negative regulating protein (MDBP-2) from rooster liver nuclear extracts binds preferentially to a methylated promoter region 5'TTCACCTTmCGCTATGAGGGGGATCATACTGG3' of the avian vitellogenin II gene...
Zingg, J M, Alva, G P, Jost, J P
We have isolated the mouse MyoD1 gene flanked by its promoter region by screening a genomic library with synthetic oligonucleotides. The structural gene is interrupted by two G + C rich introns....
An estrogen-dependent protein present in chicken liver polysomes binds to the 5' untranslated region of the chicken vitellogenin II mRNA. Competition binding assays with different RNAs indicate that...
Pawlak, A, Bryans, M, Jost, J P
In vitro transcription competition with oligonucleotides has shown that a down regulating factor can be displaced by a methylated oligonucleotide covering a specific region of the avian vitellogenin...
In gel shift assays, affinity chromatography-purified NHP1 forms a stable complex with different sequences of the chicken vitellogenin II gene. The apparent KD of NHP1 with the estrogen response...
In vitro RNA synthesis and expression of vitellogenin gene in isolated chicken liver nuclei.
Optimal conditions for prolonged in vitro synthesis of RNA in isolated chicken liver nuclei have been described. It is shown by incorporation of gamma32P-GTP into RNA, analysis of the product on...
Identification of a large precursor of vitellogenin mRNA in the liver of estradiol-treated chicks.
Jost, J P, Pehling, G, Ohno, T, Cozens, P
Studies were performed to determine whether vitellogenin mRNA from avian liver has a precursor molecule or not. Total cellular RNA was prepared from estradiol-treated chicken liver in the presence of...
Jost, J P, Seldran, M, Geiser, M
DNA-cellulose competition binding assays were used to measure the ability of cloned DNA fragments of the chicken vitellogenin II gene to displace the estrogen-receptor complex from total chicken DNA...
Estrogen induces a demethylation at the 5' end region of the chicken vitellogenin gene.
Wilks, A F, Cozens, P J, Mattaj, I W, Jost, J P
The two isoschizomeric restriction endonucleases Msp I and Hpa II have been used to study changes in the methylation pattern of the vitellogenin gene after estrogen treatment. In the liver of the...
Saluz, H P, Jiricny, J, Jost, J P
Genomic sequencing was used to study the extent of cytosine methylation of four CpG sites within the regulatory region of the estradiol-inducible avian vitellogenin II gene. Three of these sites, two...
Jost, J P, Geiser, M, Seldran, M
Avian vitellogenin-cauliflower mosaic virus hybrid gene is effectively transcribed in vitro in the homologous embryonic liver nuclei system. The transcription of the hybrid gene is modulated by the...
MyoD1 promoter autoregulation is mediated by two proximal E-boxes.
Zingg, J M, Pedraza-Alva, G, Jost, J P
We show that in mouse myoblasts the MyoD1 promoter is highly stimulated by MyoD1 expression, suggesting that it is controlled by a positive feedback loop. Using deletion and mutation analyses, we...
Supercoiled DNA loops linked to the nuclear matrix can be progressively cleaved with deoxyribonuclease I. The DNA which remains associated with the nuclear matrix can be purified and analysed for...
DNA binding and methyl transfer catalysed by mouse DNA methyltransferase.
Reale, A, Lindsay, H, Saluz, H P, Pradhan, S, Adams, R L, Jost, J P, ...
By using a purified fraction of mouse DNA methyltransferase we have shown, by gel-retardation analysis, that the enzyme forms a low-affinity complex preferentially with hemimethylated DNA; the...