J. P. Kamerling

High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG (2008)

Blanchard, V., Gadkari, R.P, George, A.V.E., Roy, R., Gerwig, G.J., Leeflang, B.R., ...

The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoproteins. With the aim to generate biologically active 15Nlabeled glycohormones for conformational...

Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris (2007)

Blanchard, Véronique, Gadkari, R.A., Gerwig, G.J., Leeflang, B.R., Dighe, R.R., Kamerling, J.P.

Human chorionic gonadotropin (hCG) is a heterodimeric, placental glycoprotein hormone involved in the maintenance of the corpus luteum during the first trimester of pregnancy. Biologically active hCG...

A lectin array-based methodology for the analysis of protein glycosylation (2007)

Rosenfeld, R., Bangio, H., Gerwig, G.J., Rosenberg, R., Aloni, R., Cohen, Y., ...

Glycosylation is the most versatile and one of the most abundant protein modifications. It has a structural role as well as diverse functional roles in many specific biological functions, including...

Conformational studies on five octasaccharides isolated from chondroitin sulfate using NMR spectroscopy and molecular modeling (2007)

Blanchard, Véronique, Chevalier, F., Imberty, A., Leeflang, B.R., Sugahara, K., Kamerling, J.P.

Chondroitin sulfate proteoglycans (CS-PG) are involved in the regulation of the central nervous system in vertebrates due to their presence on cell surfaces and in the extracellular matrix of...

N-glycans of recombinant human acid α-glucosidase expressed in the milk of transgenic rabbits (2007)

Jongen, S.P., Gerwig, G.J., Leeflang, B.R., Koles, K., Mannesse, M.L.M, ...

Pompe disease is a lysosomal glycogen storage disorder characterized by acid α-glucosidase (GAA) deficiency. More than 110 different pathogenic mutations in the gene encoding GAA have been observed....

Evaluation of protein N-glycosylation in 2-DE : erythropoietin as a study case (2007)

Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., ...

The structure, function, and physico-chemical properties of many proteins are determined by PTM, being glycosylation the most complex. This study describes how a combination of typical proteomics...

Dominant antibody responses to Fucα1-3GalNAc and Fucα1-2Fucα1-3GlcNAc containing carbohydrate epitopes in Pan troglodytes vaccinated and infected with Schistosoma mansoni (2006)

Remoortere, A. Van, Vermeer, H.J., Roon, A.M., Langermans, J.A., Thomas, A.W., Wilson, R.A., ...

Critical elements of oligosaccharide acceptor substrates for the Pasteurella multocida hyaluronan synthase (2006)

Williams, K.J., Halkes, K.M., Kamerling, J.P., DeAngelis, P.L.

Probing the substrate specificity of four different sialyltransferases using synthetic β-D-Galp-(1→4)-β-D-GlcpNAc-(1→2)-α-D-Manp-(1→O) (CH2)7CH3 analogues: General activating effect of replacing N-acetylglucosamine by N-propionylglucosamine (2006)

Rohfritsch, P.F., Joosten, J.A.F., Krzewinski-Recchi, M.A., Harduin-Lepers, A., Laporte, B., Juliant, S., ...

Structural analysis of naturally occurring sialic acids (2006)

Kamerling, J.P., Gerwig, G.J.

Mechanism of action of the endo-(1→3)-α-glucanase MutAp from the mycoparasitic fungus Trichoderma harzianum (2006)

Grun, C.H., Dekker, N., Nieuwland, A.A., Klis, F.M., Kamerling, J.P., Vliegenthart, J.F.G., ...

Structural medicine: the Importance of glycomics for health and disease (2006)

Kamerling, J.P., Geyer, R., Leeflang, B.R., Altmann, F., Dell, A., Holst, O., ...

Conformational analysis in aqueous solution and estimation of the persistence length of exopolysaccharides produced by Lactobacillus helveticus Lh59 and Streptococcus macedonicus Sc136 (2006)

Kuik, J.A. Van, Vincent, S.J.F., Leeflang, B.R., Kamerling, J.P.

Structural characterization of the N-glycans of gpMuc from Mucuna pruriens seeds (2006)

Patrizi, L. Di, Rosati, F., Guerranti, R., Pagani, R., Gerwig, G.J., Kamerling, J.P.

Affinity determination of Ricinus communis agglutinin ligands identified from combinatorial O- and S-,N-glycopeptide libraries (2006)

Maljaars, C.E.P., Halkes, K.M., Oude, W.L., Haseley, S.R., Upton, P.J., McDonnell, M.B., ...

Mechanism of action of the endo-(1--3)-alpha-glucanase MutAp from the mycoparasitic fungus Trichoderma harzianum. (2006)

Grün, C.H., Dekker, N., Nieuwland, A.A., Klis, F.M., Kamerling, J.P., Vliegenthart, J.F.G., ...

Mechanism of action of the endo-(1-->3)-alpha-glucanase MutAp from the mycoparasitic fungus Trichoderma harzianum (2006)

Grün, C.H., Dekker, N., Nieuwland, A.A., Klis, F.M., Kamerling, J.P., Vliegenthart, J.F.G., ...

The structure of cell wall alpha-glucan from fission yeast. (2005)

Grun, C.H., Hochstenbach, F., Humbel, B.M., Verkleij, A.J., Sietsma, J.H., Klis, F.M., ...

Morphology and structural integrity of fungal cells depend on cell wall polysaccharides. The chemical structure and biosynthesis of two types of these polysaccharides, chitin and...

Nidovirus sialate-O-acetylesterases: Evolution and substrate specificity of corona- and toroviral receptor-destroying enzymes (2005)

Smits, S.L., Gerwig, G.J., Vliet, A.L.W., Lissenberg, A., Briza, P., Kamerling, J.P., ...

SPR studies of carbohydrate-protein interactions: Signal enhancement of low-molecular-mass analytes by organoplatinum(II)-labeling (2005)

Beccati, D., Halkes, K.M., Batema, G.D., Guillena Townley, G., Koten, G. Van, ...

Synthesis of differentially protected ribitol derivatives from 3,4-O-benzylidene-D-ribono-1,5-lactone (2005)

Lefeber, D.J., Steunenberg, P., Vliegenthart, J.F.G., Kamerling, J.P.

Gold glyconanoparticles as probes to explore the carbohydrate-mediated self-recognition of marine sponge cells (2005)

Halkes, K.M., Meeldijk, J.D., Verkleij, A.J., Vliegenthart, J.F.G., Kamerling, J.P.

A facile method for the preparation of gold glyconanoparticles from free oligosaccharides and their applicability in carbohydrate-protein interaction studies (2005)

Halkes, K.M., Maljaars, C.E.P., Gerwig, G.J., Kamerling, J.P.

Determination of the specificity of monoclonal antibodies against Schistosoma mansoni CAA glycoprotein antigen, using neoglycoconjugate variants (2005)

Remoortere, A. Van, Hokke, C.H., Deelder, A.M., Vliegenthart, J.F.G., Kamerling, J.P.

The structure of cell wall alpha-glucan from fission yeast (2005)

Grün, C.H., Hochstenbach, F.M.H., Humbel, B.M., Verkleij, A.J., Sietsma, J.H., Klis, F.M., ...

The structure of cell wall α-glucan from fission yeast (2005)

Grun, C.H., Hochstenbach, F., Humbel, B.M., Verkleij, A.J., Sietsma, J.H., Klis, F.M., ...

N- and O-glycans of recombinant human C1 inhibitor expressed in the milk of transgenic rabbits (2004)

Vliegenthart, J.F.G., Koles, K., Pieper, F.R., Nuijens, J.H., Mannesse, M.L.M., ...

Human C1 inhibitor (hC1INH) is a therapeutic N, O-glycoprotein with a growing number of clinical applications, but the current natural supplies are not likely to meet the clinical demands. Therefore,...

Synthesis and conjugation of oligosaccharide analogues of fragments of the immunoreactive glycan part of the circulating anodic antigen of the parasite Schistosoma mansoni (2004)

Kuil, J., Maljaars, C.E.P., Halkes, K.M., Vliegenthart, J.F.G., Kamerling, J.P.

Influence of lactation parameters on the N-glycosylation of recombinant human C1 inhibitor isolated from the milk of transgenic rabbits (2004)

Koles, K., Mannesse, M.L.M, Zoetemelk, R., Vliegenthart, J.F.G., Kamerling, J.P.

Synthesis of gold glyconanoparticles: possible probes for the exploration of carbohydrate-mediated self-recognistion of marine sponge cells (2004)

Halkes, K.M., Meeldijk, J.D., Verkleij, A.J., Vliegenthart, J.F.G., Kamerling, J.P.

Presence of bioactive glycoconjugates on different stages of saffron corm (2004)

Rubio Moraga, A., Fernandez Nohales, P., Gerwig, G.J., Escribano, J., Kamerling, J.P., Fernandez, J.A.

Identification of carbohydrates binding to lectins by using surface plasmon resonance in combination with HPLC profiling (2004)

Gutierrez Gallego, R., Haseley, S.R., Vliegenthart, J.F.G., Kamerling, J.P.

Donor specificity in the glycosylation of TAMM-Horsefall glycoprotein: Conservation of the Sda determinant in pairs of twins (2004)

Rohfritsch, P.F., Rinnbauer, M., Vliegenthart, J.F.G., Kamerling, J.P.

Screening and characterization of β-N-acetylhexosaminidases for the synthesis of nucleotide-actived disaccharides (2004)

Nieder, V., Kutzer, M., Kren, V., Gutierrez Gallego, R., Kamerling, J.P., Elling, L.

Identification of sialic acids on Leishmania donovani amastigotes (2004)

Chava, A.K., Chatterjee, M., Gerwig, G.J., Kamerling, J.P., Mandal, C.

O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma (2004)

Shen, Y., Kohla, G., Sipos, B., Kalthoff, H., Gerwig, G.J., Kamerling, J.P., ...

N- and O-glycans of recombinant human C1 inhibitor expressed in the milk of transgenic rabbits (2004)

Koles, K., Pieper, F.R., Nuijens, J.H., Mannesse, M.L.M, Vliegenthart, J.F.G., ...

Human C1 inhibitor (hC1INH) is a therapeutic N, O-glycoprotein with a growing number of clinical applications, but the current natural supplies are not likely to meet the clinical demands. Therefore,...

Chemo-enzymatic synthesis of a tetra- and octasaccharide fragment of the capsular polysaccharide of Streptococcus pneumoniae type 14 (2003)

Vliegenthart, J.F.G., Joosten, J.A.F., Kamerling, J.P.

Chemo-enzymatic synthesis of tetra-, penta-, and hexasaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 14 (2003)

Vliegenthart, J.F.G., Joosten, J.A.F., Kamerling, J.P.

Enzymatic synthesis of the core-2 sialyl Lewis X O-glycan on the tumor-associated MUC1a' peptide (2003)

Vliegenthart, J.F.G., Gutiérrez-Gallego, R., Dudziak, G., Kragl, U., Wandrey, C., Kamerling, J.P.

Synthesis of nucleotide-activated disaccharides with b-galactosidase from Bacillus circulans and a-galactosidase from Bifidobacterium adolescentis (2003)

Vliegenthart, J.F.G., Nieder, V., Marx, S.P., Gutiérrez Gallego, R., Kamerling, J.P., Elling, L.

The enzymatic synthesis of nucleotide-activated disaccharides by glycosidase-catalyzed transglycosylation reactions was investigated. High product yields were obtained when the kinetically driven...

Organoplatinum(II) complexes as a color biomarker in solid-phase pepetide chemistry and screening (2003)

Koten, G. Van, Guilena,G., Halkes, K.M., Rodriguez, G., Batema, G.D., Kamerling, J.P.

Organoplatinum(II) complexes as a color biomarker in solid-phase pepetide chemistry and screening (2003)

Guillena Townley, G., Halkes, K.M., Rodriguez Longarella, G., Batema, G.D., Koten, G. Van, Kamerling, J.P.

Enzymatic synthesis of the core-2 sialyl Lewis X O-glycan on the tumor-associated MUC1a' peptide (2003)

Gutierrez Gallego, R., Dudziak, G., Kragl, U., Wandrey, C., Kamerling, J.P., Vliegenthart, J.F.G.

Th1-directing adjuvants increase the immunogenicity of oligosaccharide-protein conjugate vaccines related to Streptococcus pneumoniae Type 3 (2003)

Lefeber, D.J., Benaissa-Trouw, B.J., Vliegenthart, J.F.G., Kamerling, J.P., Jansen, W.T.M., Kraaijeveld, C.A., ...

Identification and characterization of adsorbed serum sialoglycans on leishmania donovani promastigotes (2003)

Chatterjee, M., Kumar Chava, A., Kohla, G., Pal, S., Merling, A., Hinderlich, S., ...

Synthesis of B-D-Galp-(1->4)-B-D-GlcpNAc-(1->2)-a-D-Manp-(1->O)(CH2)7CH3 mimics to explore the substrate specificity of sialyltransferase and trans-sialidases (2003)

Joosten, J.A.F., Evers, B., Summeren, R.P., Kamerling, J.P., Vliegenthart, J.F.G.

Specific antibody responses to three schistosome-related carbohydrate structures in recently exposed immigrants and established residents in an area of Schistosoma mansoni endemicity (2003)

Naus, C.W.A., Remoortere, A. Van, Ouma, J.H., Kimani, G., Dunne, D.W., Kamerling, J.P., ...

By the use of surface plasmon resonance spectroscopy, immunoglobulin G (IgG) subclass and IgM antibodies against three schistosome-derived carbohydrate structures, FLDN...

Immunodiagnostically applicable monoclonal antibodies to the circulating anodic antigen of Schistosoma mansoni bind to small, defined oligosaccharide epitopes (2003)

Vermeer, H.J., Dam, G.J. Van, Halkes, K.M., Kamerling, J.P., Vliegenthart, J.F.G., Hokke, C.H., ...

A novel open-chain nononic acid linked by an ether bond to glucose as a polysaccharide constituent (2002)

Vliegenthart, J.F.G., Faber, E.J., Kuik, J.A. Van, Halkes, K.M., Kamerling, J.P.

A novel sugar constituent was isolated from the heteropolysaccharide excreted by Streptococcus thermophilus 8 S when grown in skimmed milk. The structure and absolute configuration were determined by...

Characterization of the exopolysaccharide produced by Streptococcus thermophilus 8S containing an open chain nononic acid (2002)

Vliegenthart, J.F.G., Faber, E.J., Haaster, D.J. Van, Kamerling, J.P.

Chemoenzymic synthesis of oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 14 (2002)

Vliegenthart, J.F.G., Michalik, D., Kamerling, J.P.

Conformational analysis of two xylose-containing N-glycans in aqueous solution by using 1H NMR ROESY and NOESY spectroscopy in combination with MD simulations (2002)

Vliegenthart, J.F.G., Lommerse, J.P.M., Kamerling, J.P.

Immunogenicity in a mouse model of a conjugate vaccine made with a synthetic single repeating unit of type 14 pneumococcal polysaccharide coupled to CRM197 (2002)

Vliegenthart, J.F.G., Mawas, F., Niggemann, J., Jones, C., Corbel, M.J., Kamerling, J.P.

Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A...

Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines (2002)

Vliegenthart, J.F.G., Lefeber, D.J., Gutiérrez Gallego, R., Grün, C.H., Proietti, D., Ascenzi, S. D', ...

A series of well-defined oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide,...

Modeling of the structure in aqueous solution of the exopolysaccharide produced by Lactobacillus helveticus 766 (2002)

Vliegenthart, J.F.G., Faber, E.J., Kuik, J.A. Van, Kamerling, J.P.

A method is described for constructing a conformational model in water of a heteropolysaccharide built up from repeating units, and is applied to the exopolysaccharide produced by Lactobacillus...

Studies on the relevance of the glycan at Asn-52 of the a-subunit of human chorionic gonadotropin in the ab dimer (2002)

Vliegenthart, J.F.G., Erbel, P.J.A., Haseley, S.R., Kamerling, J.P.

Glycosylation of Asn-52 of the a-subunit (a Asn-52) is required for bioactivity of the ab-dimeric human chorionic gonadotropin (hCG), although at a molecular level the effect of the glycan at aAsn-52...

Synthesis of a hexasaccharide fragment of the capsular polysaccharide of Streptococcus pneumoniae type 3 (2002)

Vliegenthart, J.F.G., Lefeber, D.J., Aldaba Arévalo, E., Kamerling, J.P.

Synthesis of fragments of the glycocalyx glycan of the parasite Schistosoma mansoni (2002)

Vliegenthart, J.F.G., Ágoston, K., Kerékgyártó, J., Hajkó, J., Batta, G., Lefeber, D.J., ...

The Giardia intestinalis filamentous cyst wall contains a novel b(1-3)-N-acetyl-D-galactosamine polymer : a structural and conformational study (2002)

Vliegenthart, J.F.G., Gerwig, G.J., Kuik, J.A. Van, Leeflang, B.R., Kamerling, J.P., Karr, C.D., ...

Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G....

Unravelling carbohydrate interactions with biosensors using surface plasmon resonance (SPR) detection (2002)

Vliegenthart, J.F.G., Haseley, S.R., Kamerling, J.P.

Surface plasmon resonance (SPR) is an optical phenomenon occurring at a metal coated interface between two media of different refractive index, e.g., water and glass. Exploitation of this phenomenon...

Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines (2002)

Lefeber, D.J., Gutierrez Gallego, R., Grun, C.H., D'Ascenzi, S., Costantino, P., Kamerling, J.P., ...

A series of well-defined oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide,...

Unravelling carbohydrate interactions with biosensors using Surface Plasmon Resonance (SPR) detection (2002)

Haseley, S.R., Kamerling, J.P., Vliegenthart, J.F.G.

Surface plasmon resonance (SPR) is an optical phenomenon occurring at a metal coated interface between two media of different refractive index, e.g., water and glass. Exploitation of this phenomenon...

Immunogenicity in a mouse model of a conjugate vaccine made with a synthetic single repeating unit of type 14 peumococcal polysaccharide coupled to CRM197 (2002)

Mawas, F., Niggeman, J., Jones, C., Corbel, M.J., Kamerling, J.P., Vliegenthart, J.F.G.

Characterization of the exopolysaccharide produced by Streptococcus thermophilus 8S containing an open chain nononic acid (2002)

Faber, E.J., Haaster, D.J. Van, Kamerling, J.P., Vliegenthart, J.F.G.

Chemoenzymatic synthesis of oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 14 (2002)

Michalik, D., Vliegenthart, J.F.G., Kamerling, J.P.

The Giardia intestinalis filamentous cyst wall contains a novel b(1-3)-N-acetyl-D-galactosamine polymer: a structural and conformational study (2002)

Gerwig, G.J., Kuik, J.A. Van, Leeflang, B.R., Kamerling, J.P., Vliegenthart, J.F.G., Karr, C.D., ...

Conformational analysis of two xylose-containing N-glycans in aqueous solution by using 1H NMR ROESY and NOESY spectroscopy in combination with MD simulations (2002)

Lommerse, J.P.M., Kamerling, J.P., Vliegenthart, J.F.G.

Studies on the relevance of the glycan at Asn-52 of the a-subunit of human chorionic gonadotropin in the ab dimer (2002)

Erbel, P.J.A., Haseley, S.R., Kamerling, J.P., Vliegenthart, J.F.G.

Glycosylation of Asn-52 of the a-subunit (a Asn-52) is required for bioactivity of the ab-dimeric human chorionic gonadotropin (hCG), although at a molecular level the effect of the glycan at aAsn-52...

Synthesis of a hexasaccharide fragment of the capsular polysaccharide of Streptococcus Pneumoniae type 3 (2002)

Lefeber, D.J., Aldaba Arevalo, E., Kamerling, J.P.,