A. Pringle, D. M. Baker, J. L. Platt, J. P. Wares, J. P. Latgé, J. W. Taylor
Microbes and other organisms smaller than one to a few millimeters in size are hypothesized to have global populations, in contrast to the geographically restricted ranges of larger organisms....
Rat monoclonal antibodies against Aspergillus galactomannan (1992)
Sarfati, J, Goris, A, Prévost, M C, Lesourd, M, Kamphuis, H, ...
Monoclonal antibodies (MAbs) against Aspergillus fumigatus galactomannan were produced in rats. Seven of them, EB-A1 through EB-A7, were characterized in more detail. They were all immunoglobulin M...
Glucan Synthase Complex of Aspergillus fumigatus
Beauvais, A., Bruneau, J. M., Mol, P. C., Buitrago, M. J., Legrand, R., Latgé, J. P.
The glucan synthase complex of the human pathogenic mold Aspergillus fumigatus has been investigated. The genes encoding the putative catalytic subunit Fks1p and four Rho proteins of A. fumigatus...
Paugam, A., Sarfati, J., Romieu, R., Viguier, M., Dupouy-Camet, J., Latgé, J. P.
With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an...
Phagocytosis and Intracellular Fate of Aspergillus fumigatus Conidia in Alveolar Macrophages
Ibrahim-Granet, O., Philippe, B., Boleti, H., Boisvieux-Ulrich, E., Grenet, D., Stern, M., ...
Aspergillus fumigatus is the most prevalent airborne fungal pathogen responsible for fatal invasive aspergillosis in immunocompromised patients. Upon arrival in the lung alveolus, conidia of A....
Philippe, B., Ibrahim-Granet, O., Prévost, M. C., Gougerot-Pocidalo, M. A., Sanchez Perez, M., ...
Phagocytosis and mechanisms of killing of Aspergillus fumigatus conidia by murine alveolar macrophages (AM), which are the main phagocytic cells of the innate immunity of the lung, were investigated....
Molecular characterization of the food-borne fungus Neosartorya fischeri (Malloch and Cain).
Girardin, H, Monod, M, Latgé, J P
The food-borne fungus Neosartorya fischeri, which is phenotypically related to the human opportunistic pathogen Aspergillus fumigatus, causes spoilage of heat-processed fruit products. Genomic...
Internalization of Aspergillus fumigatus conidia by epithelial and endothelial cells.
Paris, S, Boisvieux-Ulrich, E, Crestani, B, Houcine, O, Taramelli, D, Lombardi, L, ...
The internalization of conidia of the opportunistic fungus Aspergillus fumigatus by primary cell cultures of nonprofessional phagocytes was investigated. This study is the first to show that A....
Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans.
Beauvais, A, Monod, M, Wyniger, J, Debeaupuis, J P, Grouzmann, E, Brakch, N, ...
A dipeptidyl-peptidase IV was purified from the culture medium of the human-pathogenic fungus Aspergillus fumigatus. The enzyme has an apparent molecular mass of 95 kDa and contained approximately 10...
Genetic diversity among clinical and environmental isolates of Aspergillus fumigatus.
Debeaupuis, J P, Sarfati, J, Chazalet, V, Latgé, J P
To determine if cases of invasive aspergillosis (IA) were caused by strains of Aspergillus fumigatus with unique characteristics, strains from immunosuppressed patients with IA were compared to...
Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus.
Calera, J A, Paris, S, Monod, M, Hamilton, A J, Debeaupuis, J P, Diaquin, M, ...
Aspergillus fumigatus possesses two catalases (described as fast and slow on the basis of their electrophoretic mobility). The slow catalase has been recognized as a diagnostic antigen for...
Th1 and Th2 cytokines in mice with invasive aspergillosis.
Cenci, E, Perito, S, Enssle, K H, Mosci, P, Latgé, J P, Romani, L, ...
With a murine model of invasive aspergillosis we investigated cytokine production by CD4+ T helper cells and the effects of cytokine administration or neutralization on the course and outcome of...
Fontaine, T, Hartland, R P, Diaquin, M, Simenel, C, Latgé, J P
Two exo-beta-1,3-glucanases (herein designated exoG-I and exoG-II) were isolated from the cell wall autolysate of the filamentous fungus Aspergillus fumigatus and purified by ion-exchange,...
Stynen, D, Goris, A, Sarfati, J, Latgé, J P
A double-direct sandwich enzyme-linked immunosorbent assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoassay, which detects...
Verweij, P E, Latgé, J P, Rijs, A J, Melchers, W J, De Pauw, B E, Hoogkamp-Korstanje, J A, ...
The performance of a sandwich enzyme-linked immunosorbent assay (ELISA) which detects Aspergillus galactomannan (GM) was evaluated in bronchoalveolar lavage (BAL) fluid samples from 19 patients who...
Verweij, P E, Meis, J F, Sarfati, J, Hoogkamp-Korstanje, J A, Latgé, J P, Melchers, W J
Twenty-three sequential Aspergillus fumigatus sputum isolates, which had been collected over a period of 2 years, from two patients with cystic fibrosis were genotyped by random amplified polymorphic...
Interactions between conidia of Aspergillus fumigatus and human complement component C3.
Activation and deposition of C3 on Aspergillus fumigatus conidia have been previously demonstrated. This study investigated in further detail the interactions between complement component C3 and the...
Rat monoclonal antibodies against Aspergillus galactomannan.
Stynen, D, Sarfati, J, Goris, A, Prévost, M C, Lesourd, M, Kamphuis, H, ...
Monoclonal antibodies (MAbs) against Aspergillus fumigatus galactomannan were produced in rats. Seven of them, EB-A1 through EB-A7, were characterized in more detail. They were all immunoglobulin M...
The 18-kilodalton antigen secreted by Aspergillus fumigatus.
Latgé, J P, Moutaouakil, M, Debeaupuis, J P, Bouchara, J P, Haynes, K, Prévost, M C
One of the major antigens secreted in vitro by Aspergillus fumigatus is an 18-kDa basic protein which has been purified by cation-exchange chromatography. It is recognized by sera from aspergilloma...
Molecular epidemiology of nosocomial invasive aspergillosis.
Girardin, H, Sarfati, J, Traoré, F, Dupouy Camet, J, Derouin, F, Latgé, J P
Moderately repeated DNA sequences were used to fingerprint strains of Aspergillus fumigatus isolated from patients with invasive aspergillosis and their hospital environment. Most strains sampled...
Development of DNA probes for fingerprinting Aspergillus fumigatus.
Girardin, H, Latgé, J P, Srikantha, T, Morrow, B, Soll, D R
Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of...
Isolation and characterization of a secreted metalloprotease of Aspergillus fumigatus.
Monod, M, Paris, S, Sanglard, D, Jaton-Ogay, K, Bille, J, Latgé, J P
A metalloprotease (MEP) secreted by Aspergillus fumigatus was isolated from an alkaline protease-deficient mutant after the fungus was cultivated in the presence of collagen as the sole nitrogen and...
rodletless mutants of Aspergillus fumigatus.
Thau, N, Monod, M, Crestani, B, Rolland, C, Tronchin, G, Latgé, J P, ...
Conidia of Aspergillus fumigatus adhere in vitro to host proteins and cells via the outer cell wall layer. The rodA gene of A. fumigatus was cloned by homology with the rodA gene of Aspergillus...
Latgé, J P, Kobayashi, H, Debeaupuis, J P, Diaquin, M, Sarfati, J, Wieruszeski, J M, ...
The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the culture filtrate....
Two α(1-3) Glucan Synthases with Different Functions in Aspergillus fumigatus
Beauvais, A., Maubon, D., Park, S., Morelle, W., Tanguy, M., Huerre, M., ...
α(1-3) glucan is a main component of the Aspergillus fumigatus cell wall. In spite of its importance, synthesis of this amorphous polymer has not been investigated to date. Two genes in A....
Mouyna, I, Monod, M, Fontaine, T, Henrissat, B, Léchenne, B, Latgé, J P
A new family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases (Gelp), recently identified and characterized in the filamentous fungus Aspergillus fumigatus, showed functional...
Glucan Synthase Complex of Aspergillus fumigatus
Beauvais, A., Bruneau, J. M., Mol, P. C., Buitrago, M. J., Legrand, R., Latgé, J. P.
The glucan synthase complex of the human pathogenic mold Aspergillus fumigatus has been investigated. The genes encoding the putative catalytic subunit Fks1p and four Rho proteins of A. fumigatus...
Paugam, A., Sarfati, J., Romieu, R., Viguier, M., Dupouy-Camet, J., Latgé, J. P.
With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an...
Phagocytosis and Intracellular Fate of Aspergillus fumigatus Conidia in Alveolar Macrophages
Ibrahim-Granet, O., Philippe, B., Boleti, H., Boisvieux-Ulrich, E., Grenet, D., Stern, M., ...
Aspergillus fumigatus is the most prevalent airborne fungal pathogen responsible for fatal invasive aspergillosis in immunocompromised patients. Upon arrival in the lung alveolus, conidia of A....
Philippe, B., Ibrahim-Granet, O., Prévost, M. C., Gougerot-Pocidalo, M. A., Sanchez Perez, M., ...
Phagocytosis and mechanisms of killing of Aspergillus fumigatus conidia by murine alveolar macrophages (AM), which are the main phagocytic cells of the innate immunity of the lung, were investigated....
Molecular characterization of the food-borne fungus Neosartorya fischeri (Malloch and Cain).
Girardin, H, Monod, M, Latgé, J P
The food-borne fungus Neosartorya fischeri, which is phenotypically related to the human opportunistic pathogen Aspergillus fumigatus, causes spoilage of heat-processed fruit products. Genomic...
Internalization of Aspergillus fumigatus conidia by epithelial and endothelial cells.
Paris, S, Boisvieux-Ulrich, E, Crestani, B, Houcine, O, Taramelli, D, Lombardi, L, ...
The internalization of conidia of the opportunistic fungus Aspergillus fumigatus by primary cell cultures of nonprofessional phagocytes was investigated. This study is the first to show that A....
Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans.
Beauvais, A, Monod, M, Wyniger, J, Debeaupuis, J P, Grouzmann, E, Brakch, N, ...
A dipeptidyl-peptidase IV was purified from the culture medium of the human-pathogenic fungus Aspergillus fumigatus. The enzyme has an apparent molecular mass of 95 kDa and contained approximately 10...
Genetic diversity among clinical and environmental isolates of Aspergillus fumigatus.
Debeaupuis, J P, Sarfati, J, Chazalet, V, Latgé, J P
To determine if cases of invasive aspergillosis (IA) were caused by strains of Aspergillus fumigatus with unique characteristics, strains from immunosuppressed patients with IA were compared to...
Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus.
Calera, J A, Paris, S, Monod, M, Hamilton, A J, Debeaupuis, J P, Diaquin, M, ...
Aspergillus fumigatus possesses two catalases (described as fast and slow on the basis of their electrophoretic mobility). The slow catalase has been recognized as a diagnostic antigen for...
Th1 and Th2 cytokines in mice with invasive aspergillosis.
Cenci, E, Perito, S, Enssle, K H, Mosci, P, Latgé, J P, Romani, L, ...
With a murine model of invasive aspergillosis we investigated cytokine production by CD4+ T helper cells and the effects of cytokine administration or neutralization on the course and outcome of...
Fontaine, T, Hartland, R P, Diaquin, M, Simenel, C, Latgé, J P
Two exo-beta-1,3-glucanases (herein designated exoG-I and exoG-II) were isolated from the cell wall autolysate of the filamentous fungus Aspergillus fumigatus and purified by ion-exchange,...
Stynen, D, Goris, A, Sarfati, J, Latgé, J P
A double-direct sandwich enzyme-linked immunosorbent assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoassay, which detects...
Verweij, P E, Latgé, J P, Rijs, A J, Melchers, W J, De Pauw, B E, Hoogkamp-Korstanje, J A, ...
The performance of a sandwich enzyme-linked immunosorbent assay (ELISA) which detects Aspergillus galactomannan (GM) was evaluated in bronchoalveolar lavage (BAL) fluid samples from 19 patients who...
Verweij, P E, Meis, J F, Sarfati, J, Hoogkamp-Korstanje, J A, Latgé, J P, Melchers, W J
Twenty-three sequential Aspergillus fumigatus sputum isolates, which had been collected over a period of 2 years, from two patients with cystic fibrosis were genotyped by random amplified polymorphic...
Interactions between conidia of Aspergillus fumigatus and human complement component C3.
Activation and deposition of C3 on Aspergillus fumigatus conidia have been previously demonstrated. This study investigated in further detail the interactions between complement component C3 and the...
Rat monoclonal antibodies against Aspergillus galactomannan.
Stynen, D, Sarfati, J, Goris, A, Prévost, M C, Lesourd, M, Kamphuis, H, ...
Monoclonal antibodies (MAbs) against Aspergillus fumigatus galactomannan were produced in rats. Seven of them, EB-A1 through EB-A7, were characterized in more detail. They were all immunoglobulin M...
The 18-kilodalton antigen secreted by Aspergillus fumigatus.
Latgé, J P, Moutaouakil, M, Debeaupuis, J P, Bouchara, J P, Haynes, K, Prévost, M C
One of the major antigens secreted in vitro by Aspergillus fumigatus is an 18-kDa basic protein which has been purified by cation-exchange chromatography. It is recognized by sera from aspergilloma...
Molecular epidemiology of nosocomial invasive aspergillosis.
Girardin, H, Sarfati, J, Traoré, F, Dupouy Camet, J, Derouin, F, Latgé, J P
Moderately repeated DNA sequences were used to fingerprint strains of Aspergillus fumigatus isolated from patients with invasive aspergillosis and their hospital environment. Most strains sampled...
Development of DNA probes for fingerprinting Aspergillus fumigatus.
Girardin, H, Latgé, J P, Srikantha, T, Morrow, B, Soll, D R
Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of...
Isolation and characterization of a secreted metalloprotease of Aspergillus fumigatus.
Monod, M, Paris, S, Sanglard, D, Jaton-Ogay, K, Bille, J, Latgé, J P
A metalloprotease (MEP) secreted by Aspergillus fumigatus was isolated from an alkaline protease-deficient mutant after the fungus was cultivated in the presence of collagen as the sole nitrogen and...
rodletless mutants of Aspergillus fumigatus.
Thau, N, Monod, M, Crestani, B, Rolland, C, Tronchin, G, Latgé, J P, ...
Conidia of Aspergillus fumigatus adhere in vitro to host proteins and cells via the outer cell wall layer. The rodA gene of A. fumigatus was cloned by homology with the rodA gene of Aspergillus...
Latgé, J P, Kobayashi, H, Debeaupuis, J P, Diaquin, M, Sarfati, J, Wieruszeski, J M, ...
The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the culture filtrate....
Two α(1-3) Glucan Synthases with Different Functions in Aspergillus fumigatus
Beauvais, A., Maubon, D., Park, S., Morelle, W., Tanguy, M., Huerre, M., ...
α(1-3) glucan is a main component of the Aspergillus fumigatus cell wall. In spite of its importance, synthesis of this amorphous polymer has not been investigated to date. Two genes in A....
Mouyna, I, Monod, M, Fontaine, T, Henrissat, B, Léchenne, B, Latgé, J P
A new family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases (Gelp), recently identified and characterized in the filamentous fungus Aspergillus fumigatus, showed functional...