J. P. Liautard

Cloning and characterization of the Brucella ovis heat shock protein DnaK functionally expressed in Escherichia coli.

Cellier, M F, Teyssier, J, Nicolas, M, Liautard, J P, Marti, J, Sri Widada, J

The Brucella ovis dnaK gene, homolog to the eukaryotic hsp70 genes, was cloned by using a Drosophila melanogaster probe. Comparison of B. ovis and Escherichia coli sequences revealed a similar...

Live Brucella spp. fail to induce tumor necrosis factor alpha excretion upon infection of U937-derived phagocytes.

Caron, E, Peyrard, T, Köhler, S, Cabane, S, Liautard, J P, Dornand, J

Tumor necrosis factor alpha (TNF-alpha) plays a central role in activation of first-line defenses of a host against foreign organisms. To determine whether Brucella infection modulated TNF-alpha...

Mouse DNA sequences complementary to small nuclear RNA U1.

Piechaczyk, M, Lelay-Taha, M N, Sri-Widada, J, Brunel, C, Liautard, J P, Jeanteur, P

A mouse genomic library was screened for sequences complementary to U1 nuclear RNA. Out of the eight clones tested, none contained more than one copy of U1. Six of them were identical and one of...

In vitro translation studies of the cytoplasmic nonpolysomal particles containing messenger RNA.

Liautard, J P, Egly, J M

We analyzed the translational capacity of different kinds of free cytoplasmic messenger ribonucleoprotein complexes (free mRNP) in a Hela cell cell free system. Native free mRNP are not translated...

Interaction of snRNAs with rapidly sedimenting nuclear sub-structures (hnRNPs) from HeLa cells.

Sri-Widada, J, Liautard, J P, Brunel, C, Jeanteur, P

We have shown previously (Liautard et al., 1982, J. Mol. Biol., 162, 623-643) that digestion with micrococcal nuclease under drastic conditions of a pure U1 snRNP, as well as a mixture containing U2,...

Purification and characterization of a simple ribonucleoprotein particle containing small nucleoplasmic RNAs (snRNP) as a subset of RNP containing heterogenous nuclear RNA (hnRNP) from HeLa cells.

Brunel, C, Widada, J S, Lelay, M N, Jeanteur, P, Liautard, J P

A ribonucleoprotein complex whose RNA complement consists exclusively of small nuclear RNA species (snRNA) has been purified from particles containing heterogenous nuclear RNA (hnRNP) from HeLa...

Purification of histone messenger ribonucleoprotein particles from HeLa cell S-phase polysomes. Characterization of associated proteins

Liautard, J.P., Jeanteur, Ph.

We have purified HeLa histone mRNA from polysomes of S-phase cells which had been synchronized by hydroxyurea treatment. This mRNA was shown to direct the in vitro synthesis of all five histones...

Cloning and characterization of the Brucella ovis heat shock protein DnaK functionally expressed in Escherichia coli.

Cellier, M F, Teyssier, J, Nicolas, M, Liautard, J P, Marti, J, Sri Widada, J

The Brucella ovis dnaK gene, homolog to the eukaryotic hsp70 genes, was cloned by using a Drosophila melanogaster probe. Comparison of B. ovis and Escherichia coli sequences revealed a similar...

Live Brucella spp. fail to induce tumor necrosis factor alpha excretion upon infection of U937-derived phagocytes.

Caron, E, Peyrard, T, Köhler, S, Cabane, S, Liautard, J P, Dornand, J

Tumor necrosis factor alpha (TNF-alpha) plays a central role in activation of first-line defenses of a host against foreign organisms. To determine whether Brucella infection modulated TNF-alpha...

Mouse DNA sequences complementary to small nuclear RNA U1.

Piechaczyk, M, Lelay-Taha, M N, Sri-Widada, J, Brunel, C, Liautard, J P, Jeanteur, P

A mouse genomic library was screened for sequences complementary to U1 nuclear RNA. Out of the eight clones tested, none contained more than one copy of U1. Six of them were identical and one of...

In vitro translation studies of the cytoplasmic nonpolysomal particles containing messenger RNA.

Liautard, J P, Egly, J M

We analyzed the translational capacity of different kinds of free cytoplasmic messenger ribonucleoprotein complexes (free mRNP) in a Hela cell cell free system. Native free mRNP are not translated...

Interaction of snRNAs with rapidly sedimenting nuclear sub-structures (hnRNPs) from HeLa cells.

Sri-Widada, J, Liautard, J P, Brunel, C, Jeanteur, P

We have shown previously (Liautard et al., 1982, J. Mol. Biol., 162, 623-643) that digestion with micrococcal nuclease under drastic conditions of a pure U1 snRNP, as well as a mixture containing U2,...

Purification and characterization of a simple ribonucleoprotein particle containing small nucleoplasmic RNAs (snRNP) as a subset of RNP containing heterogenous nuclear RNA (hnRNP) from HeLa cells.

Brunel, C, Widada, J S, Lelay, M N, Jeanteur, P, Liautard, J P

A ribonucleoprotein complex whose RNA complement consists exclusively of small nuclear RNA species (snRNA) has been purified from particles containing heterogenous nuclear RNA (hnRNP) from HeLa...

Purification of histone messenger ribonucleoprotein particles from HeLa cell S-phase polysomes. Characterization of associated proteins

Liautard, J.P., Jeanteur, Ph.

We have purified HeLa histone mRNA from polysomes of S-phase cells which had been synchronized by hydroxyurea treatment. This mRNA was shown to direct the in vitro synthesis of all five histones...

Activation of human Vγ9Vδ2 T cells by a Brucella suis non-peptidic fraction impairs bacterial intracellular multiplication in monocytic infected cells

Ottones, F, Liautard, J, Gross, A, Rabenoelina, F, Liautard, J P, Favero, J

Human γδ T cells are considered to play an important role in the early response to infection with intracellular pathogens. Evidence has been presented that the percentage of γδ T cells with...