Multiplex approaches for biochemical TSE characteisation and differentiation (2008)
Thorne, J.K., Whatling, K.L., Jacobs, J.G., Erkens, J.H.F., Langeveld, J.P.M., Sauer, M.J.
Atypical status of bovine spongiform encephalopathy in Poland: a molecular typing study (2008)
Polak, M.P., Zmudzinski, J.F., Jacobs, J.G., Langeveld, J.P.M.
Bovine spongiform encephalopathy in Sweden: an H-type variant (2008)
Gavier-Widen, D., Noremark, M., Langeveld, J.P.M., Stack, M., Biacabe, A.G., Vulin, J., ...
Bovine spongiform encephalopathy (BSE) had never been detected in Sweden until 2006, when the active surveillance identified a case in a 12-year-old cow. The case was an unusual form, because several...
Beyond PrPresres type1/type2 dichotomy in Creutzfeldt-Jakob Disease (2008)
Uro-Coste, E., Cassard, H., Simon, S., Lugan, S., Bilheude, J.M., Perret-Liaudet, A., ...
Methionine Sulfoxides on PrPSc: A Prion-Specific Covalent Signature (2008)
Canello, T., Engelstein, R., Moshel, O., Xanthopoulos, K., Langeveld, J.P.M., Sklaviadis, T., ...
Prion diseases are fatal neurodegenerative disorders believed to be transmitted by PrPSc, an aberrant form of the membrane protein PrPC. In the absence of an established form-specific covalent...
Antibodies for discriminatory preclinical diagnosis of ovine scrapie and BSE (2008)
Jacobs, J.G., Erkens, J.H.F., Riepema, K.H., Bossers, A., ...
Unusual migration pattern of PrPres from bovine brainstem tissue (2008)
Polak, M.P., Zmudzinski, J.F., Larska, M., Erkens, J.H.F., ...
Characterization of an atypical type-H BSE case in Sweden (2008)
Gavier-Widen, D., Renstrom, L., Noremark, M., Otander, L., Klingeborn, B., Baron, T., ...
Atypical scrapie in Italy: immunohistochemical study with a panel of different antibodies (2008)
Lulini, B., Perazzini, A.Z., Porcario, C., Corona, C., Langeveld, J.P.M.
Strain variability in bovine atypical TSEs (2008)
Baron, T., Biacabe, A.G., Bencsik, A., Jacobs, J.G., Langeveld, J.P.M., Acutis, P.L., ...
Espinosa, J.C., Andreoletti, O., Castilla, J., Herva, M.E., Morales, M., Alamillo, E., ...
Sheep can be experimentally infected with bovine spongiform encephalopathy (BSE), and the ensuing disease is similar to scrapie in terms of pathogenesis and clinical signs. BSE infection in sheep is...
Notari, S., Capellari, S., Langeveld, J.P.M., Giese, A., Strammiello, R., Gambetti, P., ...
Molecular typing in Creutzfeldt¿Jakob disease (CJD) relies on the detection of distinct protease-resistant prion protein (PrPSc) core fragments, which differ in molecular mass or glycoform ratio....
Mapping of possible prion protein self interaction domains using peptide arrays (2007)
Rigter, A., Langeveld, J.P.M., Timmers-Parohi, D., Jacobs, J.G., Bossers, A.
Background The common event in transmissible spongiform encephalopathies (TSEs) or prion diseases is the conversion of host-encoded protease sensitive cellular prion protein (PrPC) into strain...
Enzymatic degradation of PrPSc fails to dis-infect bovine BSE brain homogenates (2007)
Langeveld, J.P.M., Groschup, M.H., Buschmann, A., Becher, D., Wang, J.K., Shih, J.C.H.
Immunohistochemical features of an H-type case of bovine spongiform encephalopathy in Sweden (2007)
Gavier-Widen, D., Ottander, L., Langeveld, J.P.M., Westergren, E., Baron, T.
A diferent antigenic isoform of PrPSc in species other than mice (2007)
Canello, T., Engelstein, R., Gasset, M., Sklaviadis, T., Xanthopoulos, K., Langeveld, J.P.M., ...
Antonis, A.F.G., Bruschke, C.J.M., Rueda, P., Maranga, L., Casal, J., Vela, C., ...
A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested...
Detection of type 1 prion protein in variant Creutzfeldt-Jakob disease (2006)
Yull, H.M., Ritchie, D.L., Langeveld, J.P.M., Bruce, M.E., Ironside, J.W., ...
Molecular typing of the abnormal form of the prion protein (PrPSc) has come to be regarded as a powerful tool in the investigation of the prion diseases. All evidence thus far presented indicates a...
Jeffrey, M., Martin, S., Gonzalez, L., Foster, J., Langeveld, J.P.M., ...
Scrapie is a transmissible spongiform encephalopathy (TSE) or prion disease, which naturally affects sheep and goats. Immunohistochemical epitope mapping of abnormal PrP accumulations (PrPd) in brain...
Langeveld, J.P.M., Jacobs, J.G., Erkens, J.H.F., Bossers, A.
Background Diagnosis based on prion detection in lymph nodes of sheep and goats can improve active surveillance for scrapie and, if it were circulating, for bovine spongiform encephalopathy (BSE)....
Transmission of new bovine prion to mice (2006)
Baron, T.G.M., Biacabe, A.G., Bencsik, A., Langeveld, J.P.M.
Biswas, S., Langeveld, J.P.M., Tipper, D., Lu, S.
Prion diseases are fatal neurodegenerative disorders characterized by the accumulation of an abnormal isoform (PrPSc) of the normal cellular prion protein (PrPC) in the brain. Reportedly, abnormal...
Gretzschel, A., Buschmann, A., Langeveld, J.P.M., Groschup, M.H.
After the implementation of an active surveillance programme for scrapie in sheep in the EU, the number of diagnosed classical scrapie cases rose sharply and a novel kind of so-called atypical...
Raymond, G.J., Olsen, E.A., Lee, K.S., Raymond, L.D., Bryant, P.K., Baron, G.S., ...
Chronic wasting disease (CWD) is an emerging transmissible spongiform encephalopathy (prion disease) of North American cervids, i.e., mule deer, white-tailed deer, and elk (wapiti). To facilitate in...
Sharpe, A., McElroy, M., Langeveld, J.P.M., Bassett, H., O'Donoghue, A.M., Sweeney, T.
Since scrapie and bovine spongiform encephalopathy (BSE) in sheep are clinicopathologically indistinguishable, BSE in sheep may have been misdiagnosed as scrapie. Disease-specific prion protein...
Veerhuis, R., Boshuizen, R.S., Morbin, M., Mazzoleni, G., Hoozemans, J.J., Langedijk, J.P., ...
Complement activation products C1q and C3d, serum amyloid P component (SAP) and activated glial cells accumulate in amyloid deposits of conformationally changed prion protein (PrPSc) in...
Thuring, C.M.A., Langeveld, J.P.M., Vromans, M.E.W., Sweeney, T.
Sheep are susceptible experimentally to bovine spongiform encephalopathy (BSE), the clinical signs being indistinguishable from those of scrapie. Because of the possibility of natural ovine BSE...
Sharpe, A., McElroy, M., Langeveld, J.P.M., Bassett, H., O'Donoghue, A.M., Sweeney, T.
Since scrapie and bovine spongiform encephalopathy (BSE) in sheep are clinicopathologically indistinguishable, BSE in sheep may have been misdiagnosed as scrapie. Disease-specific prion protein...
Multiple PrPSc conformations in variant Creutzfeldt-Jakob disease (2005)
Head, M.W., Yull, H.M., Ritchie, D.L., Langeveld, J.P.M., Bruce, M.E., ...
Boshuizen, R.S., Langeveld, J.P.M., Salmona, M., Williams, A., Meloen, R.H., Langendijk, J.P.
Transmissible spongiform encephalopathies are neurodegenerative diseases and are considered to be caused by malformed prion proteins accumulated into fibrillar structures that can then aggregate to...
Manipulation of PrPres production in scrapie-infected neuroblastoma cells (2004)
Bate, C., Langeveld, J.P.M., Williams, A.
In the present study the accumulation of protease resistant prion protein (PrPres) in scrapie-infected neuroblastoma cells (ScN2a cells) was shown to be dependent on culture conditions. The highest...
Sachsamanoglou, M., Paspaltzis, I., Petrakis, S., Verghese-Nikolakaki, S., Panagiotidis, C.H., Voitlander, T., ...
We describe the quality of a rabbit polyclonal antiserum (Sal1) that was raised against mature human recombinant prion protein (rhuPrP). Epitope mapping demonstrated that the Sal1 antiserum...
Thuring, C.M.A., Erkens, J.H.F., Jacobs, J.G., Bossers, A., Garssen, G.J., ...
A procedure for discrimination between scrapie and bovine spongiform encephalopathy (BSE) in sheep is of importance for establishing whether BSE has entered the sheep population. Since BSE has not...
Enzymatic degradation of prion protein in brain stem from infected cattle and sheep (2003)
Langeveld, J.P.M., Wang, J.J., Shih, G.C., Garssen, G.J., Bossers, A., ...
Prions¿infectious agents involved in transmissible spongiform encephalopathies¿normally survive proteolytic and mild protein-destructive processes. Using bacterial keratinase produced by Bacillus...
Large scale production and downstream processing of a recombinant porcine parvovirus vaccine (2002)
Maranga, L., Rueda, P., Antonis, A.F.G., Vela, C., Langeveld, J.P.M., Casal, J.I., ...
Porcine parvovirus (PPV) virus-like particles (VLPs) constitute a potential vaccine for prevention of parvovirus-induced reproductive failure in gilts. Here we report the development of a large scale...
Veerhuis, R., Hoozemans, J.J.M., Janssen, I., Boshuizen, R.S., Langeveld, J.P.M., Eikelenboom, P.
Prion diseases are characterized by accumulation of protease resistant isoforms of prion protein (termed PrPSC), glial activation and neurodegeneration. The time course of PrP deposition, appearance...
PrPCWD lymphoid cell targets in early and advanced chronic wasting disease of mule deer (2002)
Sigurdson, C.J., Barillas-Mury, C., Miller, M.W., Oesch, B., Langeveld, J.P.M., ...
Up to 15% of free-ranging mule deer in northeastern Colorado and southeastern Wyoming, USA, are afflicted with a prion disease, or transmissible spongiform encephalopathy (TSE), known as chronic...
Bate, C., Boshuizen, R.S., Langeveld, J.P.M., Williams, A.
Previous studies have demonstrated a role for microglia in the neuronal loss that occurs in the transmissible spongiform encephalopathies or prion diseases. In the present studies, the processes that...
Oral immunisation of naive and primed animals with transgenic potato tubers expressing LT-B (2001)
Lauterslager, T.G.M., Florack, D.E.A., Molthoff, J.W., Langeveld, J.P.M., Bosch, D., ...
The efficacy of edible vaccines produced in potato tubers was examined in mice. Transgenic plants were developed by Agrobacterium tumefaciens-mediated transformation. The antigen selected was the...
Schaaper, W.M.M., Langeveld, J.P.M., Boshuizen, R.S., Meloen, R.H.
The influence of the nature of the bond between a peptide and a (lipidic) carrier molecule on the immunogenicity of that construct was investigated. As types of bonds a thioester-, a disulfide-, an...
Oral immunisation of naive and primed animals with transgenic potato tubers expressing LT-B (2001)
Lauterslager, T.G.M., Florack, D.E.A., Molthoff, J.W., Langeveld, J.P.M., Bosch, D., ...
Diagnosis of bovine spongiform encephalopathy: a review (2000)
Langeveld, J.P.M., Garssen, G.J., Jacobs, J.G., Schreuder, B.E.C., Smits, M.A.
Rijsewijk, F.A.M., Kaashoek, M.J., Langeveld, J.P.M., Meloen, R., Judek, J., Bienkowska-Szewczyk, K., ...
Rapid assay for BSE and scrapie (1999)
Garssen, G.J., Jacobs, J.G., Smits, M.A., Schreuder, B.E.C., Langeveld, J.P.M.
Martinez-Torrecuadrada, J.L., Langeveld, J.P.M., Venteo, A., Sanz, A., Dalsgaard, K., Hamilton, W.D.O., ...
Scrapie-aasociated prion protein in the gastro-intestinal tract of sheep with natural scrapie (1999)
Schreuder, B.E.C., Vromans, M.E.W., Langeveld, J.P.M., Smits, M.A.
Pathogenesis of natural scrapie in sheep (1999)
Schreuder, B.E.C., Vromans, M.E.W., Langeveld, J.P.M., Smits, M.A.
Tonsillar biopsy and PrPSc detection in the preclinical diagnosis of scrapie (1998)
Schreuder, B.E.C., Vromans, M.E.W., Langeveld, J.P.M., Smits, M.A.