J. Pablo Radicella

APE1 overexpression in XRCC1-deficient cells complements the defective repair of oxidative single strand breaks but increases genomic instability (2005)

Sossou, Marguerite, Flohr-Beckhaus, Claudia, Schulz, Ina, Daboussi, Fayza, Epe, Bernd, Radicella, J. Pablo

XRCC1 protein is essential for mammalian viability and is required for the efficient repair of single strand breaks (SSBs) and damaged bases in DNA. XRCC1-deficient cells are genetically unstable and...

Poly(ADP-ribosyl)ation accelerates DNA repair in a pathway dependent on Cockayne syndrome B protein (2003)

Flohr, Claudia, Bürkle, Alexander, Radicella, J. Pablo, Epe, Bernd

Activation of poly(ADP‐ribose)polymerases 1 and 2 (PARP‐1 and PARP‐2) is one of the earliest responses of mammalian cells to DNA damage by numerous genotoxic agents. We have analysed...

Identification of KIN (KIN17), a Human Gene Encoding a Nuclear DNA-Binding Protein, as a Novel Component of the TP53-Independent Response to Ionizing Radiation (2001)

Christel Masson, Farid Menaa, Ghislaine Pinon-Lataillade, Yveline Frobert, J. Pablo Radicella, Jaime F. Angulo

Masson, C., Menaa, F., Pinon-Lataillade, G., Frobert, Y., Radicella, J. P. and Angulo, J. F. Identification of KIN (KIN17), a Human Gene Encoding a Nuclear DNA-Binding Protein, as a Novel Component...

Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: bypass of the AP lyase activity step (2001)

Vidal, Antonio E., Hickson, IanD., Boiteux, Serge, Radicella, J.Pablo

The generation of reactive oxygen species in the cell provokes, among other lesions, the formation of 8-oxo-7,8-dihydroguanine (8-oxoG) in DNA. Due to mispairing with adenine during replication,...

Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: bypass of the AP lyase activity step (2001)

Vidal, Antonio E., Hickson, Ian D., Boiteux, Serge, Radicella, J. Pablo

The generation of reactive oxygen species in the cell provokes, among other lesions, the formation of 8-oxo-7,8-dihydroguanine (8-oxoG) in DNA. Due to mispairing with adenine during replication,...

Effect of single mutations in the OGG1 gene found in human tumors on the substrate specificity of the Ogg1 protein (2000)

Audebert, Marc, Radicella, J. Pablo, Dizdaroglu, Miral

We have investigated the effect of single amino acid substitutions of conserved arginines on the catalytic activities of the human Ogg1 protein (α-hOgg1-Ser326) (wild-type α-hOgg1). Mutant forms of...

Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines (2000)

Dherin, Claudine, Dizdaroglu, Miral, Doerflinger, Hélène, Boiteux, Serge, Radicella, J. Pablo

In Drosophila, the S3 ribosomal protein has been shown to act as a DNA glycosylase/AP lyase capable of releasing 8-hydroxyguanine (8-OH-Gua) in damaged DNA. Here we describe a second Drosophila...

Overexpression of Ogg1 in mammalian cells: effects on induced and spontaneous oxidative DNA damage and mutagenesis (1999)

Hollenbach, Stephan, Dhénaut, Andreia, Eckert, Inge, Radicella, J. Pablo, Epe, Bernd

Chinese hamster ovary cell lines (AA8 and AS52) were stably transfected to overexpress hOgg1 protein, the human DNA repair glycosylase for 7,8-dihydro-8-oxoguanine (8-oxoG). In the transfectants, the...

Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae

Radicella, J. Pablo, Dherin, Claudine, Desmaze, Chantal, Fox, Maurice S., Boiteux, Serge

The OGG1 gene of Saccharomyces cerevisiae encodes a DNA glycosylase activity that is a functional analog of the Fpg protein from Escherichia coli and excises 7,8-dihydro-8-oxoguanine (8-oxoG) from...

Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: bypass of the AP lyase activity step

Vidal, Antonio E., Hickson, Ian D., Boiteux, Serge, Radicella, J. Pablo

The generation of reactive oxygen species in the cell provokes, among other lesions, the formation of 8-oxo-7,8-dihydroguanine (8-oxoG) in DNA. Due to mispairing with adenine during replication,...

Effect of single mutations in the OGG1 gene found in human tumors on the substrate specificity of the Ogg1 protein

Audebert, Marc, Radicella, J. Pablo, Dizdaroglu, Miral

We have investigated the effect of single amino acid substitutions of conserved arginines on the catalytic activities of the human Ogg1 protein (α-hOgg1-Ser326) (wild-type α-hOgg1). Mutant forms of...

Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines

Dherin, Claudine, Dizdaroglu, Miral, Doerflinger, Hélène, Boiteux, Serge, Radicella, J. Pablo

In Drosophila, the S3 ribosomal protein has been shown to act as a DNA glycosylase/AP lyase capable of releasing 8-hydroxyguanine (8-OH-Gua) in damaged DNA. Here we describe a second Drosophila...

XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein–protein interactions

Vidal, Antonio E., Boiteux, Serge, Hickson, Ian D., Radicella, J.Pablo

The major human AP endonuclease APE1 (HAP1, APEX, Ref1) initiates the repair of abasic sites generated either spontaneously, from attack of bases by free radicals, or during the course of the repair...

Global genome repair is required to activate KIN17, a UVC-responsive gene involved in DNA replication

Masson, Christel, Menaa, Farid, Pinon-Lataillade, Ghislaine, Frobert, Yveline, Chevillard, Sylvie, Radicella, J. Pablo, ...

UV light provokes DNA lesions that interfere with replication and transcription. These lesions may compromise cell viability and usually are removed by nucleotide excision repair (NER). In humans,...

Pathogen DNA as target for host-generated oxidative stress: Role for repair of bacterial DNA damage in Helicobacter pylori colonization

O'Rourke, Eyleen J., Chevalier, Catherine, Pinto, A. Viviana, Thiberge, Jean Michel, Ielpi, Luis, Labigne, Agnès, ...

Helicobacter pylori elicits an oxidative stress during host colonization. This oxidative stress is known to cause lesions in the host DNA. Here we addressed the question as to whether the pathogen...

Poly(ADP-ribosyl)ation accelerates DNA repair in a pathway dependent on Cockayne syndrome B protein

Flohr, Claudia, Bürkle, Alexander, Radicella, J. Pablo, Epe, Bernd

Activation of poly(ADP-ribose)polymerases 1 and 2 (PARP-1 and PARP-2) is one of the earliest responses of mammalian cells to DNA damage by numerous genotoxic agents. We have analysed the influence of...

Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases

Eichman, Brandt F., O’Rourke, Eyleen J., Radicella, J.Pablo, Ellenberger, Tom

DNA glycosylases catalyze the excision of chemically modified bases from DNA. Although most glycosylases are specific to a particular base, the 3-methyladenine (m3A) DNA glycosylases include both...

APE1 overexpression in XRCC1-deficient cells complements the defective repair of oxidative single strand breaks but increases genomic instability

Sossou, Marguerite, Flohr-Beckhaus, Claudia, Schulz, Ina, Daboussi, Fayza, Epe, Bernd, Radicella, J. Pablo

XRCC1 protein is essential for mammalian viability and is required for the efficient repair of single strand breaks (SSBs) and damaged bases in DNA. XRCC1-deficient cells are genetically unstable and...

Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae

Radicella, J. Pablo, Dherin, Claudine, Desmaze, Chantal, Fox, Maurice S., Boiteux, Serge

The OGG1 gene of Saccharomyces cerevisiae encodes a DNA glycosylase activity that is a functional analog of the Fpg protein from Escherichia coli and excises 7,8-dihydro-8-oxoguanine (8-oxoG) from...

Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: bypass of the AP lyase activity step

Vidal, Antonio E., Hickson, Ian D., Boiteux, Serge, Radicella, J. Pablo

The generation of reactive oxygen species in the cell provokes, among other lesions, the formation of 8-oxo-7,8-dihydroguanine (8-oxoG) in DNA. Due to mispairing with adenine during replication,...

Effect of single mutations in the OGG1 gene found in human tumors on the substrate specificity of the Ogg1 protein

Audebert, Marc, Radicella, J. Pablo, Dizdaroglu, Miral

We have investigated the effect of single amino acid substitutions of conserved arginines on the catalytic activities of the human Ogg1 protein (α-hOgg1-Ser326) (wild-type α-hOgg1). Mutant forms of...

Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines

Dherin, Claudine, Dizdaroglu, Miral, Doerflinger, Hélène, Boiteux, Serge, Radicella, J. Pablo

In Drosophila, the S3 ribosomal protein has been shown to act as a DNA glycosylase/AP lyase capable of releasing 8-hydroxyguanine (8-OH-Gua) in damaged DNA. Here we describe a second Drosophila...

XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein–protein interactions

Vidal, Antonio E., Boiteux, Serge, Hickson, Ian D., Radicella, J.Pablo

The major human AP endonuclease APE1 (HAP1, APEX, Ref1) initiates the repair of abasic sites generated either spontaneously, from attack of bases by free radicals, or during the course of the repair...

Global genome repair is required to activate KIN17, a UVC-responsive gene involved in DNA replication

Masson, Christel, Menaa, Farid, Pinon-Lataillade, Ghislaine, Frobert, Yveline, Chevillard, Sylvie, Radicella, J. Pablo, ...

UV light provokes DNA lesions that interfere with replication and transcription. These lesions may compromise cell viability and usually are removed by nucleotide excision repair (NER). In humans,...

Pathogen DNA as target for host-generated oxidative stress: Role for repair of bacterial DNA damage in Helicobacter pylori colonization

O'Rourke, Eyleen J., Chevalier, Catherine, Pinto, A. Viviana, Thiberge, Jean Michel, Ielpi, Luis, Labigne, Agnès, ...

Helicobacter pylori elicits an oxidative stress during host colonization. This oxidative stress is known to cause lesions in the host DNA. Here we addressed the question as to whether the pathogen...

Poly(ADP-ribosyl)ation accelerates DNA repair in a pathway dependent on Cockayne syndrome B protein

Flohr, Claudia, Bürkle, Alexander, Radicella, J. Pablo, Epe, Bernd

Activation of poly(ADP-ribose)polymerases 1 and 2 (PARP-1 and PARP-2) is one of the earliest responses of mammalian cells to DNA damage by numerous genotoxic agents. We have analysed the influence of...

Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases

Eichman, Brandt F., O’Rourke, Eyleen J., Radicella, J.Pablo, Ellenberger, Tom

DNA glycosylases catalyze the excision of chemically modified bases from DNA. Although most glycosylases are specific to a particular base, the 3-methyladenine (m3A) DNA glycosylases include both...

APE1 overexpression in XRCC1-deficient cells complements the defective repair of oxidative single strand breaks but increases genomic instability

Sossou, Marguerite, Flohr-Beckhaus, Claudia, Schulz, Ina, Daboussi, Fayza, Epe, Bernd, Radicella, J. Pablo

XRCC1 protein is essential for mammalian viability and is required for the efficient repair of single strand breaks (SSBs) and damaged bases in DNA. XRCC1-deficient cells are genetically unstable and...

Redox Regulation of Human OGG1 Activity in Response to Cellular Oxidative Stress▿

Bravard, Anne, Vacher, Monique, Gouget, Barbara, Coutant, Alexandre, De Boisferon, Florence Hillairet, Marsin, Stéphanie, ...

8-Oxoguanine (8-oxoG), a common and mutagenic form of oxidized guanine in DNA, is eliminated mainly through base excision repair. In human cells its repair is initiated by human OGG1 (hOGG1), an...

Helicobacter pylori Genes Involved in Avoidance of Mutations Induced by 8-Oxoguanine▿

Mathieu, Aurélie, O'Rourke, Eyleen J., Radicella, J. Pablo

Chromosomal rearrangements and base substitutions contribute to the large intraspecies genetic diversity of Helicobacter pylori. Here we explored the base excision repair pathway for the highly...

Unveiling Novel RecO Distant Orthologues Involved in Homologous Recombination

Marsin, Stéphanie, Mathieu, Aurélie, Kortulewski, Thierry, Guérois, Raphaël, Radicella, J. Pablo

The generation of a RecA filament on single-stranded DNA is a critical step in homologous recombination. Two main pathways leading to the formation of the nucleofilament have been identified in...

Expression of 8-oxoguanine DNA glycosylase (Ogg1) in mouse retina

Bigot, Karine, Leemput, Julia, Vacher, Monique, Campalans, Anna, Radicella, J. Pablo, Lacassagne, Emmanuelle, ...

APE1/Ref-1 Interacts with NPM1 within Nucleoli and Plays a Role in the rRNA Quality Control Process ▿ †

Vascotto, Carlo, Fantini, Damiano, Romanello, Milena, Cesaratto, Laura, Deganuto, Marta, Leonardi, Antonio, ...

APE1/Ref-1 (hereafter, APE1), a DNA repair enzyme and a transcriptional coactivator, is a vital protein in mammals. Its role in controlling cell growth and the molecular mechanisms that fine-tune its...