Athanasopoulos, V., Praszkier, J., Pittard, A. J.
Replication of the IncL/M plasmid pMU604 is controlled by a small antisense RNA molecule (RNAI), which, by inhibiting the formation of an RNA pseudoknot, regulates translation of the replication...
Role of CIS in Replication of an IncB Plasmid
Replication of the IncB plasmid pMU720 requires the synthesis of the cis-acting RepA protein and the presence of two DNA elements, ori and CIS. CIS is the 166-bp sequence separating the RepA coding...
Effect of CIS on Activity in trans of the Replication Initiator Protein of an IncB Plasmid
Praszkier, J., Murthy, S., Pittard, A. J.
RepA, the replication initiator protein of the IncB plasmid pMU720, acts preferentially in cis. The cis activity of RepA is thought to be mediated by CIS, a 166-bp region of DNA separating the coding...
Replication of the IncB miniplasmid pMU720 requires synthesis of the replication initiator protein, RepA, whose translation is coupled to that of a leader peptide, RepB. The unusual feature of this...
Interaction of the Initiator Protein of an IncB Plasmid with Its Origin of DNA Replication
Betteridge, T., Yang, J., Pittard, A. J., Praszkier, J.
The replication initiator protein RepA of the IncB plasmid pMU720 was purified and used in DNase I protection assays in vitro. RepA protected a 68-bp region of the origin of replication of pMU720....
The replication of an IncL/M plasmid is subject to antisense control.
Athanasopoulos, V, Praszkier, J, Pittard, A J
A 2,385-bp sequence that contains the information for the autonomous replication of the IncL/M plasmid pMU604 was characterized. Genetic analyses revealed that the replicon specifies at least four...
Wilson, I W, Siemering, K R, Praszkier, J, Pittard, A J
Replication of the IncB miniplasmid pMU720 is dependent on the expression of repA, the gene encoding replication initiator protein RepA. Binding of a small antisense RNA (RNAI) to its complementary...
Molecular analysis and characterization of a broad-host-range plasmid, pEP2.
Zhang, Y, Praszkier, J, Hodgson, A, Pittard, A J
Plasmid pEP2 was found to encode a protein, RepA, which is essential and rate limiting for its replication in Escherichia coli and Corynebacterium pseudotuberculosis. Mutations which altered the rate...
Molecular analysis of RNAI control of repB translation in IncB plasmids.
Wilson, I W, Praszkier, J, Pittard, A J
The translation of RepA, the replication initiation protein of the IncB plasmid pMU720, requires that its mRNA (RNAII) folds to form a pseudoknot immediately upstream of the repA Shine-Dalgarno...
Siemering, K R, Praszkier, J, Pittard, A J
Physical analysis of RNA I, the small antisense RNA which regulates the replication of IncB miniplasmid pMU720, showed that it is a highly structured molecule containing an imperfectly paired stem...
Siemering, K R, Praszkier, J, Pittard, A J
The replication frequency of the IncB miniplasmid pMU720 is dependent upon the expression of the repA gene. Binding of a small, highly structured, antisense RNA (RNA I) to its complementary target in...
Mutations affecting translational coupling between the rep genes of an IncB miniplasmid.
Praszkier, J, Wilson, I W, Pittard, A J
The nature of translational coupling between repB and repA, the overlapping rep genes of the IncB plasmid pMU720, was examined. Mutations in the start codon of the promoter proximal gene, repB,...
Mutations affecting pseudoknot control of the replication of B group plasmids.
Wilson, I W, Praszkier, J, Pittard, A J
The translational initiation region of the mRNA for the replication initiation protein (RepA) of pMU720 is predicted to be sequestered in an inhibitory secondary structure designated stem-loop III....
Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids.
Praszkier, J, Wei, T, Siemering, K, Pittard, J
Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720....
Role of countertranscript RNA in the copy number control system of an IncB miniplasmid.
Praszkier, J, Bird, P, Nikoletti, S, Pittard, J
Transcriptional mapping studies of the IncB minireplicon pMU720 demonstrated the existence of a long RNA molecule, RNA II, whose 5' portion is complementary to the product of the incompatibility gene...
Analysis of the incompatibility determinants of I-complex plasmids.
Nikoletti, S, Bird, P, Praszkier, J, Pittard, J
The isolation and characterization of minireplicons corresponding to group B and I-complex plasmids are reported. The molecular structures of the small RNAs that may play a major role in the...
Isolation and characterization of temperature-sensitive mutants of adenovirus type 7.
Fifty temperature-sensitive mutants, which replicate at 32 degrees C but not at 39.5 degrees C, were isolated after mutagenesis of the vaccine strain of adenovirus type 7 with hydroxylamine (mutation...
Role of RepA and DnaA Proteins in the Opening of the Origin of DNA Replication of an IncB Plasmid
Betteridge, T., Yang, J., Pittard, A. J., Praszkier, J.
The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia...
Evidence that there are only two tRNA(Phe) genes in Escherichia coli.
Pittard, J, Praszkier, J, Certoma, A, Eggertsson, G, Gowrishankar, J, Narasaiah, G, ...
pheV, one of the genes that code for tRNA(Phe), was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate...
Athanasopoulos, V., Praszkier, J., Pittard, A. J.
Replication of the IncL/M plasmid pMU604 is controlled by a small antisense RNA molecule (RNAI), which, by inhibiting the formation of an RNA pseudoknot, regulates translation of the replication...
Role of CIS in Replication of an IncB Plasmid
Replication of the IncB plasmid pMU720 requires the synthesis of the cis-acting RepA protein and the presence of two DNA elements, ori and CIS. CIS is the 166-bp sequence separating the RepA coding...
Effect of CIS on Activity in trans of the Replication Initiator Protein of an IncB Plasmid
Praszkier, J., Murthy, S., Pittard, A. J.
RepA, the replication initiator protein of the IncB plasmid pMU720, acts preferentially in cis. The cis activity of RepA is thought to be mediated by CIS, a 166-bp region of DNA separating the coding...
Replication of the IncB miniplasmid pMU720 requires synthesis of the replication initiator protein, RepA, whose translation is coupled to that of a leader peptide, RepB. The unusual feature of this...
Interaction of the Initiator Protein of an IncB Plasmid with Its Origin of DNA Replication
Betteridge, T., Yang, J., Pittard, A. J., Praszkier, J.
The replication initiator protein RepA of the IncB plasmid pMU720 was purified and used in DNase I protection assays in vitro. RepA protected a 68-bp region of the origin of replication of pMU720....
The replication of an IncL/M plasmid is subject to antisense control.
Athanasopoulos, V, Praszkier, J, Pittard, A J
A 2,385-bp sequence that contains the information for the autonomous replication of the IncL/M plasmid pMU604 was characterized. Genetic analyses revealed that the replicon specifies at least four...
Wilson, I W, Siemering, K R, Praszkier, J, Pittard, A J
Replication of the IncB miniplasmid pMU720 is dependent on the expression of repA, the gene encoding replication initiator protein RepA. Binding of a small antisense RNA (RNAI) to its complementary...
Molecular analysis and characterization of a broad-host-range plasmid, pEP2.
Zhang, Y, Praszkier, J, Hodgson, A, Pittard, A J
Plasmid pEP2 was found to encode a protein, RepA, which is essential and rate limiting for its replication in Escherichia coli and Corynebacterium pseudotuberculosis. Mutations which altered the rate...
Molecular analysis of RNAI control of repB translation in IncB plasmids.
Wilson, I W, Praszkier, J, Pittard, A J
The translation of RepA, the replication initiation protein of the IncB plasmid pMU720, requires that its mRNA (RNAII) folds to form a pseudoknot immediately upstream of the repA Shine-Dalgarno...
Siemering, K R, Praszkier, J, Pittard, A J
Physical analysis of RNA I, the small antisense RNA which regulates the replication of IncB miniplasmid pMU720, showed that it is a highly structured molecule containing an imperfectly paired stem...
Siemering, K R, Praszkier, J, Pittard, A J
The replication frequency of the IncB miniplasmid pMU720 is dependent upon the expression of the repA gene. Binding of a small, highly structured, antisense RNA (RNA I) to its complementary target in...
Mutations affecting translational coupling between the rep genes of an IncB miniplasmid.
Praszkier, J, Wilson, I W, Pittard, A J
The nature of translational coupling between repB and repA, the overlapping rep genes of the IncB plasmid pMU720, was examined. Mutations in the start codon of the promoter proximal gene, repB,...
Mutations affecting pseudoknot control of the replication of B group plasmids.
Wilson, I W, Praszkier, J, Pittard, A J
The translational initiation region of the mRNA for the replication initiation protein (RepA) of pMU720 is predicted to be sequestered in an inhibitory secondary structure designated stem-loop III....
Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids.
Praszkier, J, Wei, T, Siemering, K, Pittard, J
Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720....
Role of countertranscript RNA in the copy number control system of an IncB miniplasmid.
Praszkier, J, Bird, P, Nikoletti, S, Pittard, J
Transcriptional mapping studies of the IncB minireplicon pMU720 demonstrated the existence of a long RNA molecule, RNA II, whose 5' portion is complementary to the product of the incompatibility gene...
Analysis of the incompatibility determinants of I-complex plasmids.
Nikoletti, S, Bird, P, Praszkier, J, Pittard, J
The isolation and characterization of minireplicons corresponding to group B and I-complex plasmids are reported. The molecular structures of the small RNAs that may play a major role in the...
Isolation and characterization of temperature-sensitive mutants of adenovirus type 7.
Fifty temperature-sensitive mutants, which replicate at 32 degrees C but not at 39.5 degrees C, were isolated after mutagenesis of the vaccine strain of adenovirus type 7 with hydroxylamine (mutation...
Role of RepA and DnaA Proteins in the Opening of the Origin of DNA Replication of an IncB Plasmid
Betteridge, T., Yang, J., Pittard, A. J., Praszkier, J.
The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia...
Evidence that there are only two tRNA(Phe) genes in Escherichia coli.
Pittard, J, Praszkier, J, Certoma, A, Eggertsson, G, Gowrishankar, J, Narasaiah, G, ...
pheV, one of the genes that code for tRNA(Phe), was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate...