J. R. Bertino

Lack of functional retinoblastoma protein mediates increased resistance to antimetabolites in human sarcoma cell lines.

Li, W, Fan, J, Hochhauser, D, Banerjee, D, Zielinski, Z, Almasan, A, ...

Growth inhibition assays indicated that the IC50 values for methotrexate (MTX) and 5-fluorodeoxyuridine (FdUrd) in HS-18, a liposarcoma cell line lacking retinoblastoma protein (pRB), and SaOS-2, an...

Deficiency of retinoblastoma protein leads to inappropriate S-phase entry, activation of E2F-responsive genes, and apoptosis.

Almasan, A, Yin, Y, Kelly, R E, Lee, E Y, Bradley, A, Li, W, ...

The retinoblastoma susceptibility gene (Rb) participates in controlling the G1/S-phase transition, presumably by binding and inactivating E2F transcription activator family members. Mouse embryonic...

Methotrexate resistance in an in vivo mouse tumor due to a non-active-site dihydrofolate reductase mutation.

Dicker, A P, Waltham, M C, Volkenandt, M, Schweitzer, B I, Otter, G M, Schmid, F A, ...

A series of methotrexate (MTX)-resistant L1210 leukemia murine ascites tumors were developed in vivo and analyzed for drug resistance. Three of 20 tumors studied expressed an altered dihydrofolate...

Increase of dihydrofolate reductase activity in cultured mammalian cells after exposure to methotrexate.

Hillcoat, B L, Swett, V, Bertino, J R

Multidrug-resistance gene (P-glycoprotein) is expressed by endothelial cells at blood-brain barrier sites.

Cordon-Cardo, C, O'Brien, J P, Casals, D, Rittman-Grauer, L, Biedler, J L, Melamed, M R, ...

Endothelial cells of human capillary blood vessels at the blood-brain and other blood-tissue barrier sites express P-glycoprotein as detected by mouse monoclonal antibodies against the human...

THE “INDUCTION” OF DIHYDROFOLIC REDUCTASE ACTIVITY IN LEUKOCYTES AND ERYTHROCYTES OF PATIENTS TREATED WITH AMETHOPTERIN*

Bertino, J. R., Donohue, D. M., Simmons, B., Gabrio, B. W., Silber, R., Huennekens, F. M.

STUDIES ON NORMAL AND LEUKEMIC LEUKOCYTES. IV. TETRAHYDROFOLATE-DEPENDENT ENZYME SYSTEMS AND DIHYDROFOLIC REDUCTASE*

Bertino, J. R., Silber, Robert, Freeman, M., Alenty, A., Albrecht, M., Gabrio, Beverly W., ...

Methotrexate Displacement in Man*

Johns, D. G., Hollingsworth, J. W., Cashmore, A. R., Plenderleith, I. H., Bertino, J. R.

Transport characteristics of folates in cerebrospinal fluid; a study utilizing doubly labeled 5-methyltetrahydrofolate and 5-formyltetrahydrofolate

Levitt, M., Nixon, P. F., Pincus, J. H., Bertino, J. R.

Serum disappearance curves in dogs after the intravenous injection of radioactive methotrexate, 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, and folic acid followed first-order kinetics with...

Detection of point mutations in human DNA by analysis of RNA conformation polymorphism(s).

Danenberg, P V, Horikoshi, T, Volkenandt, M, Danenberg, K, Lenz, H J, Shea, L C, ...

RNA molecules were found to separate into numerous metastable conformational forms upon non-denaturing gel electrophoresis. The equilibration of the conformations was accelerated by heating or mild...

On the mechanism of selective inhibition of herpesvirus replication by (E)-5-(2-bromovinyl)-2'-deoxyuridine.

Allaudeen, H S, Kozarich, J W, Bertino, J R, De Clercq, E

Bromovinyldeoxyuridine (BVdUrd) is a potent antiherpesvirus compound with low cytotoxicity. To gain an insight into its selectivity and mechanism of inhibition, we chemically synthesized the...

Isolation of a herpesvirus-specific DNA polymerase from tissues of an American patient with Burkitt lymphoma.

Allaudeen, H S, Bertino, J R

A DNA polymerase (DNA nucleotidyltransferase) has been partially purified from a neck mass of an American patient with Burkitt lymphoma and separated from the cellular DNA polymerases. The molecular...

5-fluorouracil-methotrexate synergy: enhancement of 5-fluorodeoxyridylate binding to thymidylate synthase by dihydropteroylpolyglutamates.

Fernandes, D J, Bertino, J R

Ternary complex formation of thymidylate synthase (5,10-methylenetetrahydrofolated:dUMP C-methyltransferase, EC 2.1.1.45), 5-fluorodeoxyuridylate (FdUMP), and poly(gamma-glutamyl) conjugates of...

Formation of 10-Formylfolic Acid, a Potent Inhibitor of Dihydrofolate Reductase, in Rat Liver Slices Incubated with Folic Acid

D'Urso-Scott, M., Uhoch, J., Bertino, J. R.

During investigation of folate polyglutamate biosynthesis in rat liver slices utilizing [2-14C]folic acid, a folate compound that behaved like a polyglutamate form in the Sephadex G-15 gel filtration...

Sequence analysis of polymerase chain reaction amplified t(14;18) chromosomal breakpoints in formalin fixed, paraffin wax embedded follicular lymphoma.

Volkenandt, M, Koch, O, Fanin, R, Banerjee, D, Seger, A, Vogel, J, ...

AIMS: To determine whether junctional sequences of rearranged chromosomes can be amplified by use of the polymerase chain reaction (PCR) and whether direct sequence analysis of the PCR products is...

Lack of functional retinoblastoma protein mediates increased resistance to antimetabolites in human sarcoma cell lines.

Li, W, Fan, J, Hochhauser, D, Banerjee, D, Zielinski, Z, Almasan, A, ...

Growth inhibition assays indicated that the IC50 values for methotrexate (MTX) and 5-fluorodeoxyuridine (FdUrd) in HS-18, a liposarcoma cell line lacking retinoblastoma protein (pRB), and SaOS-2, an...

Deficiency of retinoblastoma protein leads to inappropriate S-phase entry, activation of E2F-responsive genes, and apoptosis.

Almasan, A, Yin, Y, Kelly, R E, Lee, E Y, Bradley, A, Li, W, ...

The retinoblastoma susceptibility gene (Rb) participates in controlling the G1/S-phase transition, presumably by binding and inactivating E2F transcription activator family members. Mouse embryonic...

Methotrexate resistance in an in vivo mouse tumor due to a non-active-site dihydrofolate reductase mutation.

Dicker, A P, Waltham, M C, Volkenandt, M, Schweitzer, B I, Otter, G M, Schmid, F A, ...

A series of methotrexate (MTX)-resistant L1210 leukemia murine ascites tumors were developed in vivo and analyzed for drug resistance. Three of 20 tumors studied expressed an altered dihydrofolate...

Increase of dihydrofolate reductase activity in cultured mammalian cells after exposure to methotrexate.

Hillcoat, B L, Swett, V, Bertino, J R

Multidrug-resistance gene (P-glycoprotein) is expressed by endothelial cells at blood-brain barrier sites.

Cordon-Cardo, C, O'Brien, J P, Casals, D, Rittman-Grauer, L, Biedler, J L, Melamed, M R, ...

Endothelial cells of human capillary blood vessels at the blood-brain and other blood-tissue barrier sites express P-glycoprotein as detected by mouse monoclonal antibodies against the human...

THE “INDUCTION” OF DIHYDROFOLIC REDUCTASE ACTIVITY IN LEUKOCYTES AND ERYTHROCYTES OF PATIENTS TREATED WITH AMETHOPTERIN*

Bertino, J. R., Donohue, D. M., Simmons, B., Gabrio, B. W., Silber, R., Huennekens, F. M.

STUDIES ON NORMAL AND LEUKEMIC LEUKOCYTES. IV. TETRAHYDROFOLATE-DEPENDENT ENZYME SYSTEMS AND DIHYDROFOLIC REDUCTASE*

Bertino, J. R., Silber, Robert, Freeman, M., Alenty, A., Albrecht, M., Gabrio, Beverly W., ...

Methotrexate Displacement in Man*

Johns, D. G., Hollingsworth, J. W., Cashmore, A. R., Plenderleith, I. H., Bertino, J. R.

Transport characteristics of folates in cerebrospinal fluid; a study utilizing doubly labeled 5-methyltetrahydrofolate and 5-formyltetrahydrofolate

Levitt, M., Nixon, P. F., Pincus, J. H., Bertino, J. R.

Serum disappearance curves in dogs after the intravenous injection of radioactive methotrexate, 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, and folic acid followed first-order kinetics with...

Detection of point mutations in human DNA by analysis of RNA conformation polymorphism(s).

Danenberg, P V, Horikoshi, T, Volkenandt, M, Danenberg, K, Lenz, H J, Shea, L C, ...

RNA molecules were found to separate into numerous metastable conformational forms upon non-denaturing gel electrophoresis. The equilibration of the conformations was accelerated by heating or mild...

On the mechanism of selective inhibition of herpesvirus replication by (E)-5-(2-bromovinyl)-2'-deoxyuridine.

Allaudeen, H S, Kozarich, J W, Bertino, J R, De Clercq, E

Bromovinyldeoxyuridine (BVdUrd) is a potent antiherpesvirus compound with low cytotoxicity. To gain an insight into its selectivity and mechanism of inhibition, we chemically synthesized the...

Isolation of a herpesvirus-specific DNA polymerase from tissues of an American patient with Burkitt lymphoma.

Allaudeen, H S, Bertino, J R

A DNA polymerase (DNA nucleotidyltransferase) has been partially purified from a neck mass of an American patient with Burkitt lymphoma and separated from the cellular DNA polymerases. The molecular...

5-fluorouracil-methotrexate synergy: enhancement of 5-fluorodeoxyridylate binding to thymidylate synthase by dihydropteroylpolyglutamates.

Fernandes, D J, Bertino, J R

Ternary complex formation of thymidylate synthase (5,10-methylenetetrahydrofolated:dUMP C-methyltransferase, EC 2.1.1.45), 5-fluorodeoxyuridylate (FdUMP), and poly(gamma-glutamyl) conjugates of...

Formation of 10-Formylfolic Acid, a Potent Inhibitor of Dihydrofolate Reductase, in Rat Liver Slices Incubated with Folic Acid

D'Urso-Scott, M., Uhoch, J., Bertino, J. R.

During investigation of folate polyglutamate biosynthesis in rat liver slices utilizing [2-14C]folic acid, a folate compound that behaved like a polyglutamate form in the Sephadex G-15 gel filtration...

Sequence analysis of polymerase chain reaction amplified t(14;18) chromosomal breakpoints in formalin fixed, paraffin wax embedded follicular lymphoma.

Volkenandt, M., Koch, O., Fanin, R., Banerjee, D., Seger, A., Vogel, J., ...

AIMS: To determine whether junctional sequences of rearranged chromosomes can be amplified by use of the polymerase chain reaction (PCR) and whether direct sequence analysis of the PCR products is...

Examination of the reduced affinity of the thymidylate synthase G52S mutation for FdUMP by ab initio and semi-empirical studies.

Sapse, A. M., Capiaux, G. M., Bertino, J. R.

BACKGROUND: The G52S mutation in the Arg50 loop of thymidylate synthase leads to decreased binding of FdUMP. It has been suggested that the mutation affects the Arg50 residue (within the Arg50 loop)...