J. S. Khillan

Partial rescue of a lethal phenotype of fragile bones in transgenic mice with a chimeric antisense gene directed against a mutated collagen gene.

Khillan, J S, Li, S W, Prockop, D J

Previously, transgenic mice were prepared that developed a lethal phenotype of fragile bones because they expressed an internally deleted mini-gene for the pro alpha 1(I) chain of human type I...

Expression of a partially deleted gene of human type II procollagen (COL2A1) in transgenic mice produces a chondrodysplasia.

Vandenberg, P, Khillan, J S, Prockop, D J, Helminen, H, Kontusaari, S, Ala-Kokko, L

A minigene version of the human gene for type II procollagen (COL2A1) was prepared that lacked a large central region containing 12 of the 52 exons and therefore 291 of the 1523 codons of the gene....

Transgenic mice expressing a partially deleted gene for type I procollagen (COL1A1). A breeding line with a phenotype of spontaneous fractures and decreased bone collagen and mineral.

Pereira, R, Khillan, J S, Helminen, H J, Hume, E L, Prockop, D J

A line of transgenic mice was prepared that expressed moderate levels of an internally deleted human gene for the pro alpha 1(I) chain of type I procollagen. The gene construct was modeled after a...

Phenotypic variability and incomplete penetrance of spontaneous fractures in an inbred strain of transgenic mice expressing a mutated collagen gene (COL1A1).

Pereira, R, Halford, K, Sokolov, B P, Khillan, J S, Prockop, D J

Phenotype variability and incomplete penetrance are frequently observed in human monogenic diseases such as osteogenesis imperfecta. Here an inbred strain of transgenic mice expressing an internally...

An inbred line of transgenic mice expressing an internally deleted gene for type II procollagen (COL2A1). Young mice have a variable phenotype of a chondrodysplasia and older mice have osteoarthritic changes in joints.

Helminen, H J, Kiraly, K, Pelttari, A, Tammi, M I, Vandenberg, P, Pereira, R, ...

Studies were carried out on a line of transgenic mice that expressed an internally deleted COL2A1 gene and developed a phenotype resembling human chondrodysplasias (Vandenberg et al. 1991. Proc....

Developmental and tissue-specific expression directed by the alpha 2 type I collagen promoter in transgenic mice.

Khillan, J S, Schmidt, A, Overbeek, P A, De Crombrugghe, B, Westphal, H

Eight transgenic mice were generated in which the promoter of the mouse alpha 2(I) collagen gene (nucleotides -2000 to +54), linked to the bacterial gene for chloramphenicol acetyltransferase (CAT),...

Gene transactivation mediated by the TAT gene of human immunodeficiency virus in transgenic mice.

Khillan, J S, Deen, K C, Yu, S H, Sweet, R W, Rosenberg, M, Westphal, H

Transgenic mice were generated carrying either the long terminal repeat of Human Immunodeficiency Virus fused to the bacterial chloramphenicol acetyl transferase reporter gene or a control element of...

Lens-specific expression and developmental regulation of the bacterial chloramphenicol acetyltransferase gene driven by the murine alpha A-crystallin promoter in transgenic mice.

Overbeek, P A, Chepelinsky, A B, Khillan, J S, Piatigorsky, J, Westphal, H

Two lines of transgenic mice with one to two copies of a DNA fragment containing nucleotides -364 to +45 of the murine alpha A-crystallin gene linked to the bacterial chloramphenicol...

Lack of a phenotype in transgenic mice aberrantly expressing COL2A1 mRNA because of highly selective post-transcriptional down-regulation.

Yuan, C M, Ala-Kokko, L, Le Guellec, D, Franc, S, Fertala, A, Khillan, J S, ...

We reported previously that a 1.9-kb 5'-fragment from the human COL1A1 gene drove transcription of a promoterless human COL2A1 gene in tissues of transgenic mice that normally express the COL1A1 but...

Crystallin genes: lens specificity of the murine alpha A-crystallin gene.

Chepelinsky, A B, Khillan, J S, Mahon, K A, Overbeek, P A, Westphal, H, Piatigorsky, J

The abundant soluble proteins of the eye lens, the crystallins, are encoded by several gene families which are developmentally regulated in the embryonic lens. We have studied the expression of the...

Partial rescue of a lethal phenotype of fragile bones in transgenic mice with a chimeric antisense gene directed against a mutated collagen gene.

Khillan, J S, Li, S W, Prockop, D J

Previously, transgenic mice were prepared that developed a lethal phenotype of fragile bones because they expressed an internally deleted mini-gene for the pro alpha 1(I) chain of human type I...

Expression of a partially deleted gene of human type II procollagen (COL2A1) in transgenic mice produces a chondrodysplasia.

Vandenberg, P, Khillan, J S, Prockop, D J, Helminen, H, Kontusaari, S, Ala-Kokko, L

A minigene version of the human gene for type II procollagen (COL2A1) was prepared that lacked a large central region containing 12 of the 52 exons and therefore 291 of the 1523 codons of the gene....

Transgenic mice expressing a partially deleted gene for type I procollagen (COL1A1). A breeding line with a phenotype of spontaneous fractures and decreased bone collagen and mineral.

Pereira, R, Khillan, J S, Helminen, H J, Hume, E L, Prockop, D J

A line of transgenic mice was prepared that expressed moderate levels of an internally deleted human gene for the pro alpha 1(I) chain of type I procollagen. The gene construct was modeled after a...

Phenotypic variability and incomplete penetrance of spontaneous fractures in an inbred strain of transgenic mice expressing a mutated collagen gene (COL1A1).

Pereira, R, Halford, K, Sokolov, B P, Khillan, J S, Prockop, D J

Phenotype variability and incomplete penetrance are frequently observed in human monogenic diseases such as osteogenesis imperfecta. Here an inbred strain of transgenic mice expressing an internally...

An inbred line of transgenic mice expressing an internally deleted gene for type II procollagen (COL2A1). Young mice have a variable phenotype of a chondrodysplasia and older mice have osteoarthritic changes in joints.

Helminen, H J, Kiraly, K, Pelttari, A, Tammi, M I, Vandenberg, P, Pereira, R, ...

Studies were carried out on a line of transgenic mice that expressed an internally deleted COL2A1 gene and developed a phenotype resembling human chondrodysplasias (Vandenberg et al. 1991. Proc....

Developmental and tissue-specific expression directed by the alpha 2 type I collagen promoter in transgenic mice.

Khillan, J S, Schmidt, A, Overbeek, P A, De Crombrugghe, B, Westphal, H

Eight transgenic mice were generated in which the promoter of the mouse alpha 2(I) collagen gene (nucleotides -2000 to +54), linked to the bacterial gene for chloramphenicol acetyltransferase (CAT),...

Gene transactivation mediated by the TAT gene of human immunodeficiency virus in transgenic mice.

Khillan, J S, Deen, K C, Yu, S H, Sweet, R W, Rosenberg, M, Westphal, H

Transgenic mice were generated carrying either the long terminal repeat of Human Immunodeficiency Virus fused to the bacterial chloramphenicol acetyl transferase reporter gene or a control element of...

Lens-specific expression and developmental regulation of the bacterial chloramphenicol acetyltransferase gene driven by the murine alpha A-crystallin promoter in transgenic mice.

Overbeek, P A, Chepelinsky, A B, Khillan, J S, Piatigorsky, J, Westphal, H

Two lines of transgenic mice with one to two copies of a DNA fragment containing nucleotides -364 to +45 of the murine alpha A-crystallin gene linked to the bacterial chloramphenicol...

Lack of a phenotype in transgenic mice aberrantly expressing COL2A1 mRNA because of highly selective post-transcriptional down-regulation.

Yuan, C M, Ala-Kokko, L, Le Guellec, D, Franc, S, Fertala, A, Khillan, J S, ...

We reported previously that a 1.9-kb 5'-fragment from the human COL1A1 gene drove transcription of a promoterless human COL2A1 gene in tissues of transgenic mice that normally express the COL1A1 but...

Crystallin genes: lens specificity of the murine alpha A-crystallin gene.

Chepelinsky, A B, Khillan, J S, Mahon, K A, Overbeek, P A, Westphal, H, Piatigorsky, J

The abundant soluble proteins of the eye lens, the crystallins, are encoded by several gene families which are developmentally regulated in the embryonic lens. We have studied the expression of the...