Fukushima, T, Yao, Y, Kitajima, T, Yamamoto, H, Sekiguchi, J
The original publication is available at www.springerlink.com
Wycoff,Gary, Baker,William, Sekiguchi,J., Haas,Gerry
The Final Technical Report submitted by Northrop Corporation contains a brief synopsis of the problems encountered during the design phase of the Data Handling Recording System (DHRS) program. An...
Resolution of Holliday junctions by eukaryotic DNA topoisomerase I.
Sekiguchi, J, Seeman, N C, Shuman, S
The Holliday junction, a key intermediate in both homologous and site-specific recombination, is generated by the reciprocal exchange of single strands between two DNA duplexes. Resolution of the...
Shida, T, Noda, M, Sekiguchi, J
The Escherichia coli exonuclease III (AP endonuclease VI) is a DNA-repair enzyme that hydrolyzes the phosphodiester bond 5' to an abasic site in DNA. To study how the enzyme recognizes the abasic...
Domain structure of vaccinia DNA ligase.
The 552 amino acid vaccinia virus DNA ligase consists of three structural domains defined by partial proteolysis: (i) an amino-terminal 175 amino acid segment that is susceptible to digestion with...
Mutational analysis of vaccinia virus topoisomerase identifies residues involved in DNA binding.
Vaccinia DNA topoisomerase catalyzes the cleavage and re-joining of DNA strands through a DNA-(3'-phosphotyrosyl)-enzyme intermediate formed at a specific target sequence, 5'-(C/T)CCTT downward...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Conidiogenesis and secondary metabolism in Penicillium urticae.
Submerged cultures of Penicillium urticae (NRRL 2159A) produced the antibiotics patulin and griseofulvin when grown in a glucose-nitrate medium. A high concentration of calcium (i.e., 68 mM)...
Sekiguchi, J, Akeo, K, Yamamoto, H, Khasanov, F K, Alonso, J C, Kuroda, A
DNA sequencing of a region upstream of the mms223 gene of Bacillus subtilis showed the presence of two open reading frames, orf1 and orf2, which may encode 18- and 27-kDa polypeptides, respectively....
Rashid, M H, Tamakoshi, A, Sekiguchi, J
The expression of the major vegetative phase-specific autolysin genes (cwlB [lytC] and cwlG [lytD]) was greatly reduced by mecA and mecB null mutations. In contrast to the negative effects on late...
flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.
A flaD (sinR) null mutation depressed sigD-lacZ expression only two- to fourfold, whereas a flaD1 point mutation depressed it almost completely. Introduction of pHYSigD, a sigmaD-overproducing...
Transcription of the major Bacillus subtilis autolysin gene (cwlB) was investigated. Deletion of the region upstream of the gene cluster lppX-cwbA-cwlB led to a loss of promoter activity. Primer...
Tokunaga, T, Rashid, M H, Kuroda, A, Sekiguchi, J
Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy)...
Sekiguchi, J, Katayama, S, Yamada, Y
Penicillic acid-negative mutants were obtained from a color mutant derived from Penicillium cyclopium NRRL 1888 through N-methyl-N'-nitro-N-nitrosoguanidine treatment. One mutant (SK2N6) accumulated...
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
Kuroda, A, Asami, Y, Sekiguchi, J
Southern hybridization analysis of Bacillus subtilis 168S chromosomal DNA with a Bacillus licheniformis cell wall hydrolase gene, cwlM, as a probe indicated the presence of a cwlM homolog in B....
Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene.
A major Bacillus subtilis 168S autolysin (N-acetylmuramoyl-L-alanine amidase [EC 3.5.1.28]) was purified and then cleaved with cyanogen bromide. The N-terminal amino acid sequence of one of the...
Vaccinia virus DNA ligase has an intrinsic nick-sensing function. The enzyme discriminates at the substrate binding step between a DNA containing a 5' phosphate and a DNA containing a 5' hydroxyl at...
Patulin biosynthesis: enzymatic and nonenzymatic transformations of the mycotoxin (E)-ascladiol.
Sekiguchi, J, Shimamoto, T, Yamada, Y, Gaucher, G M
A mycotoxin, (E)-ascladiol, was established as a direct precursor of patulin in cell-free preparations of Penicillium urticae patulin-minus mutants J1 and S11, but not S15. Isomerization to a side...
Genes affecting the productivity of alpha-amylase in Bacillus subtilis Marburg.
Sekiguchi, J, Takada, N, Okada, H
Genetic control of alpha-amylase (alpha-1,4-glucan glucanohydrolase, EC 3.2.1.1.) production by Bacillus subtilis 168 was studied from the standpoint that alpha-amylase production by bacteria is...
Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA.
Vaccinia DNA topoisomerase binds duplex DNA and forms a covalent adduct at sites containing a conserved sequence element 5'(C/T)CCTT decreases in the scissile strand. Distinctive aspects of...
Vaccinia DNA topoisomerase, a eukaryotic type I enzyme, binds and cleaves duplex DNA at sites containing the sequence 5'-(T/C)CCTT. We report the identification of Tyr70 as the site of contact...
Intramolecular synapsis of duplex DNA by vaccinia topoisomerase.
Shuman, S, Bear, D G, Sekiguchi, J
Complexes formed by vaccinia topoisomerase I on plasmid DNA were visualized by electron microscopy. The enzyme formed intramolecular loop structures in which non-contiguous DNA segments were synapsed...
Resolution of Holliday junctions by eukaryotic DNA topoisomerase I.
Sekiguchi, J, Seeman, N C, Shuman, S
The Holliday junction, a key intermediate in both homologous and site-specific recombination, is generated by the reciprocal exchange of single strands between two DNA duplexes. Resolution of the...
Shida, T, Noda, M, Sekiguchi, J
The Escherichia coli exonuclease III (AP endonuclease VI) is a DNA-repair enzyme that hydrolyzes the phosphodiester bond 5' to an abasic site in DNA. To study how the enzyme recognizes the abasic...
Domain structure of vaccinia DNA ligase.
The 552 amino acid vaccinia virus DNA ligase consists of three structural domains defined by partial proteolysis: (i) an amino-terminal 175 amino acid segment that is susceptible to digestion with...
Mutational analysis of vaccinia virus topoisomerase identifies residues involved in DNA binding.
Vaccinia DNA topoisomerase catalyzes the cleavage and re-joining of DNA strands through a DNA-(3'-phosphotyrosyl)-enzyme intermediate formed at a specific target sequence, 5'-(C/T)CCTT downward...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Conidiogenesis and secondary metabolism in Penicillium urticae.
Submerged cultures of Penicillium urticae (NRRL 2159A) produced the antibiotics patulin and griseofulvin when grown in a glucose-nitrate medium. A high concentration of calcium (i.e., 68 mM)...
Sekiguchi, J, Akeo, K, Yamamoto, H, Khasanov, F K, Alonso, J C, Kuroda, A
DNA sequencing of a region upstream of the mms223 gene of Bacillus subtilis showed the presence of two open reading frames, orf1 and orf2, which may encode 18- and 27-kDa polypeptides, respectively....
Rashid, M H, Tamakoshi, A, Sekiguchi, J
The expression of the major vegetative phase-specific autolysin genes (cwlB [lytC] and cwlG [lytD]) was greatly reduced by mecA and mecB null mutations. In contrast to the negative effects on late...
flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis.
A flaD (sinR) null mutation depressed sigD-lacZ expression only two- to fourfold, whereas a flaD1 point mutation depressed it almost completely. Introduction of pHYSigD, a sigmaD-overproducing...
Transcription of the major Bacillus subtilis autolysin gene (cwlB) was investigated. Deletion of the region upstream of the gene cluster lppX-cwbA-cwlB led to a loss of promoter activity. Primer...
Tokunaga, T, Rashid, M H, Kuroda, A, Sekiguchi, J
Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy)...
Sekiguchi, J, Katayama, S, Yamada, Y
Penicillic acid-negative mutants were obtained from a color mutant derived from Penicillium cyclopium NRRL 1888 through N-methyl-N'-nitro-N-nitrosoguanidine treatment. One mutant (SK2N6) accumulated...
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
Kuroda, A, Asami, Y, Sekiguchi, J
Southern hybridization analysis of Bacillus subtilis 168S chromosomal DNA with a Bacillus licheniformis cell wall hydrolase gene, cwlM, as a probe indicated the presence of a cwlM homolog in B....
Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene.
A major Bacillus subtilis 168S autolysin (N-acetylmuramoyl-L-alanine amidase [EC 3.5.1.28]) was purified and then cleaved with cyanogen bromide. The N-terminal amino acid sequence of one of the...
Vaccinia virus DNA ligase has an intrinsic nick-sensing function. The enzyme discriminates at the substrate binding step between a DNA containing a 5' phosphate and a DNA containing a 5' hydroxyl at...
Patulin biosynthesis: enzymatic and nonenzymatic transformations of the mycotoxin (E)-ascladiol.
Sekiguchi, J, Shimamoto, T, Yamada, Y, Gaucher, G M
A mycotoxin, (E)-ascladiol, was established as a direct precursor of patulin in cell-free preparations of Penicillium urticae patulin-minus mutants J1 and S11, but not S15. Isomerization to a side...
Genes affecting the productivity of alpha-amylase in Bacillus subtilis Marburg.
Sekiguchi, J, Takada, N, Okada, H
Genetic control of alpha-amylase (alpha-1,4-glucan glucanohydrolase, EC 3.2.1.1.) production by Bacillus subtilis 168 was studied from the standpoint that alpha-amylase production by bacteria is...
Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA.
Vaccinia DNA topoisomerase binds duplex DNA and forms a covalent adduct at sites containing a conserved sequence element 5'(C/T)CCTT decreases in the scissile strand. Distinctive aspects of...
Vaccinia DNA topoisomerase, a eukaryotic type I enzyme, binds and cleaves duplex DNA at sites containing the sequence 5'-(T/C)CCTT. We report the identification of Tyr70 as the site of contact...
Intramolecular synapsis of duplex DNA by vaccinia topoisomerase.
Shuman, S, Bear, D G, Sekiguchi, J
Complexes formed by vaccinia topoisomerase I on plasmid DNA were visualized by electron microscopy. The enzyme formed intramolecular loop structures in which non-contiguous DNA segments were synapsed...