Kwak, E.L., Jankowski, J., Thayer, S.P., Lauwers, G.Y., Brannigan, B.W., Harris, P.L., ...
PURPOSE: Specific activating mutations within the epidermal growth factor receptor (EGFR) identify a subset of non-small cell lung cancers with dramatic sensitivity to the specific tyrosine kinase...
Foster, R, Hu, K Q, Lu, Y, Nolan, K M, Thissen, J, Settleman, J
We have identified a human Rho protein, RhoE, which has unusual structural and biochemical properties that suggest a novel mechanism of regulation. Within a region that is highly conserved among...
The Ras-related Rho family GTPases mediate signal transduction pathways that regulate a variety of cellular processes. Like Ras, the Rho proteins (which include Rho, Rac, and CDC42) interact directly...
O'Banion, M K, Winn, V D, Settleman, J, Young, D A
We have previously observed that bovine papillomavirus type 1 (BPV-1) induces the appearance of five cellular proteins in C127 mouse fibroblasts, four of which appear to arise by altered processing...
Hwang, E S, Riese, D J, Settleman, J, Nilson, L A, Honig, J, Flynn, S, ...
Human papillomavirus (HPV) E6 and E7 oncogenes are expressed in the great majority of human cervical carcinomas, whereas the viral E2 regulatory gene is usually disrupted in these cancers. To...
Riese, D J, Settleman, J, Neary, K, DiMaio, D
The methionine codon at bovine papillomavirus type 1 nucleotide 3091 was mutated to determine whether it may serve as an initiation codon for an E2 transcriptional repressor protein and to determine...
Bovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene.
Prakash, S S, Horwitz, B H, Zibello, T, Settleman, J, DiMaio, D
We have performed transient-expression experiments with CV1 monkey kidney cells to investigate the role of the bovine papillomavirus type 1 (BPV1) E2 gene in the regulation of the E5 transforming...
Regulation of NADPH oxidase activity by Rac GTPase activating protein(s).
Heyworth, P G, Knaus, U G, Settleman, J, Curnutte, J T, Bokoch, G M
Activation of the NADPH oxidase of phagocytic cells requires the action of Rac2 or Rac1, members of the Ras superfamily of GTP-binding proteins. Rac proteins are active when in the GTP-bound form and...
To efficiently introduce bovine papillomavirus type 1 genes into cultured cells, we constructed a hybrid viral genome in which the simian virus 40 early region is replaced with a segment of the...
Settleman, J, Fazeli, A, Malicki, J, Horwitz, B H, DiMaio, D
The bovine papillomavirus (BPV) type 1 E5 gene encodes a 44-amino-acid protein that can stably transform cultured rodent cells when expressed in the absence of all other viral genes. We have...
Hariharan, I K, Hu, K Q, Asha, H, Quintanilla, A, Ezzell, R M, Settleman, J
The rho family of GTPases has been implicated in regulating changes in cell morphology in response to extracellular signals. We have cloned three widely expressed members of this family from...
The N-terminal region of GAP regulates cytoskeletal structure and cell adhesion.
McGlade, J, Brunkhorst, B, Anderson, D, Mbamalu, G, Settleman, J, Dedhar, S, ...
Ras GTPase activating protein (GAP) possesses a C-terminal domain that interacts with GTP-bound Ras, and an N-terminal region containing two SH2 domains and an SH3 domain. In addition to its...
The genetic analysis of the papillomaviruses has been hampered by the lack of mutants conditionally defective for viral biological activities. We report here the construction and characterization of...
Many cellular signaling proteins contain SH3 (Src homology 3) domains that mediate protein interactions via specific proline-containing peptides. Unlike SH2 domains, whose interactions with...
p190 RhoGAP, the major RasGAP-associated protein, binds GTP directly.
Foster, R, Hu, K Q, Shaywitz, D A, Settleman, J
In mitogenically stimulated cells, a specific complex forms between the Ras GTPase-activating protein (RasGAP) and the cellular protein p190. We have previously reported that p190 contains a...
Foster, R, Hu, K Q, Lu, Y, Nolan, K M, Thissen, J, Settleman, J
We have identified a human Rho protein, RhoE, which has unusual structural and biochemical properties that suggest a novel mechanism of regulation. Within a region that is highly conserved among...
The Ras-related Rho family GTPases mediate signal transduction pathways that regulate a variety of cellular processes. Like Ras, the Rho proteins (which include Rho, Rac, and CDC42) interact directly...
O'Banion, M K, Winn, V D, Settleman, J, Young, D A
We have previously observed that bovine papillomavirus type 1 (BPV-1) induces the appearance of five cellular proteins in C127 mouse fibroblasts, four of which appear to arise by altered processing...
Hwang, E S, Riese, D J, Settleman, J, Nilson, L A, Honig, J, Flynn, S, ...
Human papillomavirus (HPV) E6 and E7 oncogenes are expressed in the great majority of human cervical carcinomas, whereas the viral E2 regulatory gene is usually disrupted in these cancers. To...
Riese, D J, Settleman, J, Neary, K, DiMaio, D
The methionine codon at bovine papillomavirus type 1 nucleotide 3091 was mutated to determine whether it may serve as an initiation codon for an E2 transcriptional repressor protein and to determine...
Bovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene.
Prakash, S S, Horwitz, B H, Zibello, T, Settleman, J, DiMaio, D
We have performed transient-expression experiments with CV1 monkey kidney cells to investigate the role of the bovine papillomavirus type 1 (BPV1) E2 gene in the regulation of the E5 transforming...
Regulation of NADPH oxidase activity by Rac GTPase activating protein(s).
Heyworth, P G, Knaus, U G, Settleman, J, Curnutte, J T, Bokoch, G M
Activation of the NADPH oxidase of phagocytic cells requires the action of Rac2 or Rac1, members of the Ras superfamily of GTP-binding proteins. Rac proteins are active when in the GTP-bound form and...
To efficiently introduce bovine papillomavirus type 1 genes into cultured cells, we constructed a hybrid viral genome in which the simian virus 40 early region is replaced with a segment of the...
p190 RhoGAP, the major RasGAP-associated protein, binds GTP directly.
Foster, R, Hu, K Q, Shaywitz, D A, Settleman, J
In mitogenically stimulated cells, a specific complex forms between the Ras GTPase-activating protein (RasGAP) and the cellular protein p190. We have previously reported that p190 contains a...
Settleman, J, Fazeli, A, Malicki, J, Horwitz, B H, DiMaio, D
The bovine papillomavirus (BPV) type 1 E5 gene encodes a 44-amino-acid protein that can stably transform cultured rodent cells when expressed in the absence of all other viral genes. We have...
Hariharan, I K, Hu, K Q, Asha, H, Quintanilla, A, Ezzell, R M, Settleman, J
The rho family of GTPases has been implicated in regulating changes in cell morphology in response to extracellular signals. We have cloned three widely expressed members of this family from...
The N-terminal region of GAP regulates cytoskeletal structure and cell adhesion.
McGlade, J, Brunkhorst, B, Anderson, D, Mbamalu, G, Settleman, J, Dedhar, S, ...
Ras GTPase activating protein (GAP) possesses a C-terminal domain that interacts with GTP-bound Ras, and an N-terminal region containing two SH2 domains and an SH3 domain. In addition to its...
The genetic analysis of the papillomaviruses has been hampered by the lack of mutants conditionally defective for viral biological activities. We report here the construction and characterization of...
Many cellular signaling proteins contain SH3 (Src homology 3) domains that mediate protein interactions via specific proline-containing peptides. Unlike SH2 domains, whose interactions with...