J. Strassman

In vitro assembly of infectious nucleocapsids of bacteriophage phi 6: formation of a recombinant double-stranded RNA virus.

Olkkonen, V M, Gottlieb, P, Strassman, J, Qiao, X Y, Bamford, D H, Mindich, L

A system is described for assembling infectious bacteriophage phi 6 nucleocapsids in vitro. Procapsids encoded by cDNA copies of genomic segment L in Escherichia coli were used to package and...

Escherichia coli peptide methionine sulfoxide reductase gene: regulation of expression and role in protecting against oxidative damage.

Moskovitz, J, Rahman, M A, Strassman, J, Yancey, S O, Kushner, S R, Brot, N, ...

The Escherichia coli peptide methionine sulfoxide reductase gene (msrA) encodes a single-subunit polypeptide of 212 amino acid residues (M. A. Rahman, H. Nelson, H. Weissbach, and N. Brot, J. Biol....

Physical map location of the peptide methionine sulfoxide reductase gene on the Escherichia coli chromosome.

Rahman, M A, Moskovitz, J, Strassman, J, Weissbach, H, Brot, N

cDNA cloning of portions of the bacteriophage phi 6 genome.

Mindich, L, MacKenzie, G, Strassman, J, McGraw, T, Metzger, S, Romantschuk, M, ...

Phage phi 6 has a genome consisting of three pieces of double-stranded RNA. Single-stranded RNA was prepared from phi 6 nucleocapsids by in vitro transcription with the phage RNA polymerase. These...

RNA structure and heterologous recombination in the double-stranded RNA bacteriophage phi 6.

Onodera, S, Qiao, X, Gottlieb, P, Strassman, J, Frilander, M, Mindich, L

Bacteriophage phi 6 has a genome of three segments of double-stranded RNA, designated L, M, and S. A 1.2-kbp kanamycin resistance gene was inserted into segment M but was shown to be genetically...

Construction of a transducing virus from double-stranded RNA bacteriophage phi6: establishment of carrier states in host cells.

Onodera, S, Olkkonen, V M, Gottlieb, P, Strassman, J, Qiao, X Y, Bamford, D H, ...

Bacteriophage phi 6 contains three double-stranded RNA (dsRNA) genomic segments. We have constructed a plasmid that contains a cDNA copy of the middle (M) segment, with a gene for kanamycin...

Heterologous recombination in the double-stranded RNA bacteriophage phi 6.

Mindich, L, Qiao, X, Onodera, S, Gottlieb, P, Strassman, J

Bacteriophage phi 6 contains three double-stranded RNA genomic segments. We have constructed a virus with an insertion of a kanamycin resistance gene in genomic RNA segment M. The virus forms small,...

In vitro packaging and replication of individual genomic segments of bacteriophage phi 6 RNA.

Gottlieb, P, Strassman, J, Qiao, X, Frilander, M, Frucht, A, Mindich, L

The genome of bacteriophage phi 6 contains three segments of double-stranded RNA. Procapsid structures whose formation was directed by cDNA copies of the large genomic segment are capable of...

Dependence of minus-strand synthesis on complete genomic packaging in the double-stranded RNA bacteriophage phi 6.

Frilander, M, Gottlieb, P, Strassman, J, Bamford, D H, Mindich, L

Bacteriophage phi 6 has a segmented genome consisting of three pieces of double-stranded RNA (dsRNA). The viral procapsid is the structure that packages plus strands, synthesizes the complementary...

Production of a polyhedral particle in Escherichia coli from a cDNA copy of the large genomic segment of bacteriophage phi 6.

Gottlieb, P, Strassman, J, Bamford, D H, Mindich, L

A polyhedral particle that resembles in composition and structure the procapsid of bacteriophage phi 6 was produced in Escherichia coli containing cDNA copies of the entire large genomic segment...

Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase.

Mindich, L, Nemhauser, I, Gottlieb, P, Romantschuk, M, Carton, J, Frucht, S, ...

The genome of the lipid-containing bacteriophage phi 6 contains three segments of double-stranded RNA. We determined the nucleotide sequence of cDNA derived from the largest RNA segment (L). This...

Protein P4 of the bacteriophage phi 6 procapsid has a nucleoside triphosphate-binding site with associated nucleoside triphosphate phosphohydrolase activity.

Gottlieb, P, Strassman, J, Mindich, L

Bacteriophage phi 6 contains three segments of double-stranded RNA. The procapsid consists of proteins P1, P2, P4, and P7, which are encoded by the viral L segment. cDNA copies of this segment have...

In vitro replication, packaging, and transcription of the segmented double-stranded RNA genome of bacteriophage phi 6: studies with procapsids assembled from plasmid-encoded proteins.

Gottlieb, P, Strassman, J, Qiao, X Y, Frucht, A, Mindich, L

The genome of the lipid-containing bacteriophage phi 6 contains three segments of double-stranded RNA (dsRNA). We prepared cDNA copies of the viral genome and cloned this material in plasmids that...

In vitro assembly of infectious nucleocapsids of bacteriophage phi 6: formation of a recombinant double-stranded RNA virus.

Olkkonen, V M, Gottlieb, P, Strassman, J, Qiao, X Y, Bamford, D H, Mindich, L

A system is described for assembling infectious bacteriophage phi 6 nucleocapsids in vitro. Procapsids encoded by cDNA copies of genomic segment L in Escherichia coli were used to package and...

Escherichia coli peptide methionine sulfoxide reductase gene: regulation of expression and role in protecting against oxidative damage.

Moskovitz, J, Rahman, M A, Strassman, J, Yancey, S O, Kushner, S R, Brot, N, ...

The Escherichia coli peptide methionine sulfoxide reductase gene (msrA) encodes a single-subunit polypeptide of 212 amino acid residues (M. A. Rahman, H. Nelson, H. Weissbach, and N. Brot, J. Biol....

Physical map location of the peptide methionine sulfoxide reductase gene on the Escherichia coli chromosome.

Rahman, M A, Moskovitz, J, Strassman, J, Weissbach, H, Brot, N

cDNA cloning of portions of the bacteriophage phi 6 genome.

Mindich, L, MacKenzie, G, Strassman, J, McGraw, T, Metzger, S, Romantschuk, M, ...

Phage phi 6 has a genome consisting of three pieces of double-stranded RNA. Single-stranded RNA was prepared from phi 6 nucleocapsids by in vitro transcription with the phage RNA polymerase. These...

RNA structure and heterologous recombination in the double-stranded RNA bacteriophage phi 6.

Onodera, S, Qiao, X, Gottlieb, P, Strassman, J, Frilander, M, Mindich, L

Bacteriophage phi 6 has a genome of three segments of double-stranded RNA, designated L, M, and S. A 1.2-kbp kanamycin resistance gene was inserted into segment M but was shown to be genetically...

Construction of a transducing virus from double-stranded RNA bacteriophage phi6: establishment of carrier states in host cells.

Onodera, S, Olkkonen, V M, Gottlieb, P, Strassman, J, Qiao, X Y, Bamford, D H, ...

Bacteriophage phi 6 contains three double-stranded RNA (dsRNA) genomic segments. We have constructed a plasmid that contains a cDNA copy of the middle (M) segment, with a gene for kanamycin...

Heterologous recombination in the double-stranded RNA bacteriophage phi 6.

Mindich, L, Qiao, X, Onodera, S, Gottlieb, P, Strassman, J

Bacteriophage phi 6 contains three double-stranded RNA genomic segments. We have constructed a virus with an insertion of a kanamycin resistance gene in genomic RNA segment M. The virus forms small,...

In vitro packaging and replication of individual genomic segments of bacteriophage phi 6 RNA.

Gottlieb, P, Strassman, J, Qiao, X, Frilander, M, Frucht, A, Mindich, L

The genome of bacteriophage phi 6 contains three segments of double-stranded RNA. Procapsid structures whose formation was directed by cDNA copies of the large genomic segment are capable of...

Dependence of minus-strand synthesis on complete genomic packaging in the double-stranded RNA bacteriophage phi 6.

Frilander, M, Gottlieb, P, Strassman, J, Bamford, D H, Mindich, L

Bacteriophage phi 6 has a segmented genome consisting of three pieces of double-stranded RNA (dsRNA). The viral procapsid is the structure that packages plus strands, synthesizes the complementary...

Production of a polyhedral particle in Escherichia coli from a cDNA copy of the large genomic segment of bacteriophage phi 6.

Gottlieb, P, Strassman, J, Bamford, D H, Mindich, L

A polyhedral particle that resembles in composition and structure the procapsid of bacteriophage phi 6 was produced in Escherichia coli containing cDNA copies of the entire large genomic segment...

Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase.

Mindich, L, Nemhauser, I, Gottlieb, P, Romantschuk, M, Carton, J, Frucht, S, ...

The genome of the lipid-containing bacteriophage phi 6 contains three segments of double-stranded RNA. We determined the nucleotide sequence of cDNA derived from the largest RNA segment (L). This...

Protein P4 of the bacteriophage phi 6 procapsid has a nucleoside triphosphate-binding site with associated nucleoside triphosphate phosphohydrolase activity.

Gottlieb, P, Strassman, J, Mindich, L

Bacteriophage phi 6 contains three segments of double-stranded RNA. The procapsid consists of proteins P1, P2, P4, and P7, which are encoded by the viral L segment. cDNA copies of this segment have...

In vitro replication, packaging, and transcription of the segmented double-stranded RNA genome of bacteriophage phi 6: studies with procapsids assembled from plasmid-encoded proteins.

Gottlieb, P, Strassman, J, Qiao, X Y, Frucht, A, Mindich, L

The genome of the lipid-containing bacteriophage phi 6 contains three segments of double-stranded RNA (dsRNA). We prepared cDNA copies of the viral genome and cloned this material in plasmids that...