Radical changes to chlamydial taxonomy are not necessary just yet (letter and reply) (2001)
Schachter, J., Stephens, R.S., Timms, P., Kuo, C., Bavoil, P.M., Birkelund, S., ...
Chlamydia pneumoniae (TWAR) in coronary arteries of young adults (15-34 years old).
Kuo, C C, Grayston, J T, Campbell, L A, Goo, Y A, Wissler, R W, Benditt, E P
An association of Chlamydia pneumoniae with atherosclerosis of coronary and carotid arteries and aorta has been found by seroepidemiology and by demonstration of the organism in atheromata....
Malinverni, R, Kuo, C C, Campbell, L A, Lee, A, Grayston, J T
We studied the effects of two antibiotic regimens on the course of Chlamydia pneumoniae infection in the lungs of Swiss Webster mice. After intranasal challenge with isolates AR-388 (1.3 x 10(7)...
Kuo, C C, Jackson, L A, Lee, A, Grayston, J T
The in vitro susceptibilities of Chlamydia pneumoniae isolates to macrolide, tetracycline, and quinolone antibiotics were determined. Tetracycline, clarithromycin, and erythromycin had the lowest...
In vitro drug susceptibility of Chlamydia sp. strain TWAR.
The in vitro susceptibilities of eight Chlamydia sp. strain TWAR isolates were tested against tetracycline, erythromycin, penicillin, ampicillin, sulfisoxazole, and a new drug, trospectomycin. The...
Kuo, C C, Jackson, L A, Campbell, L A, Grayston, J T
Chlamydia pneumoniae (TWAR) is a recently recognized third species of the genus Chlamydia that causes acute respiratory disease. It is distinct from the other two chlamydial species that infect...
Unique ultrastructure in the elementary body of Chlamydia sp. strain TWAR.
Chi, E Y, Kuo, C C, Grayston, J T
The ultrastructure of two prototype strains (TW-183 and AR-39) of Chlamydia sp. strain TWAR was described. The TWAR elementary body (EB) demonstrated a unique morphology and structure distinct from...
Structural and antigenic analysis of Chlamydia pneumoniae.
Campbell, L A, Kuo, C C, Grayston, J T
Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and...
Ultrastructural study of entry of Chlamydia strain TWAR into HeLa cells.
Kuo, C C, Chi, E Y, Grayston, J T
Ultrastructural studies of the entry of Chlamydia strain TWAR into HeLa cells showed that the elementary bodies first attach to host cells by the pointed end, secure other binding sites on the host...
Immunotypes of Chlamydia trachomatis isolates in Seattle, Washington.
Kuo, C C, Wang, S P, Holmes, K K, Grayston, J T
The immunotype distribution of 493 Chlamydia trachomatis isolates from 467 adults and 26 infants in the Seattle area from 1965 to 1982 is presented. All except one of the isolates from adults were...
Detection of Chlamydia pneumoniae by polymerase chain reaction.
Campbell, L A, Perez Melgosa, M, Hamilton, D J, Kuo, C C, Grayston, J T
While criteria for serodiagnosis of Chlamydia pneumoniae infection are well established, isolation of the organism is often difficult. To increase detection of this organism, C. pneumoniae-specific...
Puolakkainen, M, Kuo, C C, Shor, A, Wang, S P, Grayston, J T, Campbell, L A
The antigen-specific serological response to Chlamydia pneumoniae was studied in 45 adults with coronary artery atherosclerosis and compared with that in 40 adults with acute respiratory infection....
Factors affecting viability and growth in HeLa 229 cells of Chlamydia sp. strain TWAR.
Two prototype isolates (TW-183 and AR-39) of Chlamydia sp. strain TWAR were used to study factors affecting growth of this organism in HeLa 229 cells. The results showed that an incubation...
Amino acid requirements for growth of two isolates of Chlamydia pneumoniae were studied and compared with those for one strain of Chlamydia trachomatis in a HeLa 229 cell culture. It was shown that...
Serological response to Chlamydia pneumoniae infection.
Campbell, L A, Kuo, C C, Wang, S P, Grayston, J T
The human serological response was analyzed by using sera from patients who were serologically positive but isolation negative for Chlamydia pneumoniae and from patients with proven C. pneumoniae...
Identification of a new group of Chlamydia psittaci strains called TWAR.
Kuo, C C, Chen, H H, Wang, S P, Grayston, J T
A new group of Chlamydia psittaci strains has been identified. They are called TWAR after the laboratory designation of the first two isolates. Twelve strains were isolated from pharyngeal swabs of...
Campbell, L A, Kuo, C C, Grayston, J T
Several molecular techniques were used for comparison of the novel Chlamydia agent, TWAR, with Chlamydia trachomatis and Chlamydia psittaci. Unlike all serotypes of C. trachomatis and most strains of...
A new method for determining the serovars of Chlamydia trachomatis isolates by utilizing fluorescent-antibody staining of inclusions in cell culture is described. Monoclonal antibodies which have...
Methanol, used for fixing chlamydia organisms to microscopic slides, was shown to eliminate the antigenic reactivity of Chlamydia pneumoniae elementary bodies with fluorescent antibodies. Acetone...
Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test.
Wang, S P, Kuo, C C, Grayston, J T
Chlamydia trachomatis organisms grown in HeLa 229 cell cultures were purified and formalinized for use in the micro-immunofluorescence test. As test antigens, they were stable when stored unfrozen at...
Wang, S P, Grayston, J T, Alexander, E R, Holmes, K K
A simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens has been devised as a screening test for antibody in human sera. The test differs...
A mouse model of Chlamydia pneumoniae strain TWAR pneumonitis.
Yang, Z P, Kuo, C C, Grayston, J T
Chlamydia pneumoniae is a common cause of acute respiratory infections in humans. We evaluated mice as experimental animals for C. pneumoniae. Intranasal inoculation of Swiss Webster mice with C....
Campbell, L A, Kuo, C C, Thissen, R W, Grayston, J T
Chlamydia sp. strain TWAR is a unique Chlamydia sp. that causes acute respiratory disease. A gene bank consisting of TWAR isolate AR-39 DNA in pUC19 was screened with anti-AR-39 rabbit immune sera....
Comparative infectivity of trachoma organisms in HeLa 229 cells and egg cultures.
Kuo, C C, Wang, S P, Grayston, J T
Thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in HeLa 229 cells pretreated with diethylaminoethyl-dextran to determine the relative...
Characterization of the Group Antigen of Chlamydia trachomatis
Dhir, Sanittar P., Kenny, George E., Grayston, J. T.
A lipid complement-fixing group antigen is shared by all chlamydiae including trachoma organisms. A water-soluble polysaccharide antigen was obtained by alkali saponification from partially purified...
Chlamydia pneumoniae (TWAR) in coronary arteries of young adults (15-34 years old).
Kuo, C C, Grayston, J T, Campbell, L A, Goo, Y A, Wissler, R W, Benditt, E P
An association of Chlamydia pneumoniae with atherosclerosis of coronary and carotid arteries and aorta has been found by seroepidemiology and by demonstration of the organism in atheromata....
Malinverni, R, Kuo, C C, Campbell, L A, Lee, A, Grayston, J T
We studied the effects of two antibiotic regimens on the course of Chlamydia pneumoniae infection in the lungs of Swiss Webster mice. After intranasal challenge with isolates AR-388 (1.3 x 10(7)...
Kuo, C C, Jackson, L A, Lee, A, Grayston, J T
The in vitro susceptibilities of Chlamydia pneumoniae isolates to macrolide, tetracycline, and quinolone antibiotics were determined. Tetracycline, clarithromycin, and erythromycin had the lowest...
In vitro drug susceptibility of Chlamydia sp. strain TWAR.
The in vitro susceptibilities of eight Chlamydia sp. strain TWAR isolates were tested against tetracycline, erythromycin, penicillin, ampicillin, sulfisoxazole, and a new drug, trospectomycin. The...
Kuo, C C, Jackson, L A, Campbell, L A, Grayston, J T
Chlamydia pneumoniae (TWAR) is a recently recognized third species of the genus Chlamydia that causes acute respiratory disease. It is distinct from the other two chlamydial species that infect...
Unique ultrastructure in the elementary body of Chlamydia sp. strain TWAR.
Chi, E Y, Kuo, C C, Grayston, J T
The ultrastructure of two prototype strains (TW-183 and AR-39) of Chlamydia sp. strain TWAR was described. The TWAR elementary body (EB) demonstrated a unique morphology and structure distinct from...
Structural and antigenic analysis of Chlamydia pneumoniae.
Campbell, L A, Kuo, C C, Grayston, J T
Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and...
Ultrastructural study of entry of Chlamydia strain TWAR into HeLa cells.
Kuo, C C, Chi, E Y, Grayston, J T
Ultrastructural studies of the entry of Chlamydia strain TWAR into HeLa cells showed that the elementary bodies first attach to host cells by the pointed end, secure other binding sites on the host...
Immunotypes of Chlamydia trachomatis isolates in Seattle, Washington.
Kuo, C C, Wang, S P, Holmes, K K, Grayston, J T
The immunotype distribution of 493 Chlamydia trachomatis isolates from 467 adults and 26 infants in the Seattle area from 1965 to 1982 is presented. All except one of the isolates from adults were...
Detection of Chlamydia pneumoniae by polymerase chain reaction.
Campbell, L A, Perez Melgosa, M, Hamilton, D J, Kuo, C C, Grayston, J T
While criteria for serodiagnosis of Chlamydia pneumoniae infection are well established, isolation of the organism is often difficult. To increase detection of this organism, C. pneumoniae-specific...
Puolakkainen, M, Kuo, C C, Shor, A, Wang, S P, Grayston, J T, Campbell, L A
The antigen-specific serological response to Chlamydia pneumoniae was studied in 45 adults with coronary artery atherosclerosis and compared with that in 40 adults with acute respiratory infection....
Factors affecting viability and growth in HeLa 229 cells of Chlamydia sp. strain TWAR.
Two prototype isolates (TW-183 and AR-39) of Chlamydia sp. strain TWAR were used to study factors affecting growth of this organism in HeLa 229 cells. The results showed that an incubation...
Amino acid requirements for growth of two isolates of Chlamydia pneumoniae were studied and compared with those for one strain of Chlamydia trachomatis in a HeLa 229 cell culture. It was shown that...
Serological response to Chlamydia pneumoniae infection.
Campbell, L A, Kuo, C C, Wang, S P, Grayston, J T
The human serological response was analyzed by using sera from patients who were serologically positive but isolation negative for Chlamydia pneumoniae and from patients with proven C. pneumoniae...
Identification of a new group of Chlamydia psittaci strains called TWAR.
Kuo, C C, Chen, H H, Wang, S P, Grayston, J T
A new group of Chlamydia psittaci strains has been identified. They are called TWAR after the laboratory designation of the first two isolates. Twelve strains were isolated from pharyngeal swabs of...
Campbell, L A, Kuo, C C, Grayston, J T
Several molecular techniques were used for comparison of the novel Chlamydia agent, TWAR, with Chlamydia trachomatis and Chlamydia psittaci. Unlike all serotypes of C. trachomatis and most strains of...
A new method for determining the serovars of Chlamydia trachomatis isolates by utilizing fluorescent-antibody staining of inclusions in cell culture is described. Monoclonal antibodies which have...
Methanol, used for fixing chlamydia organisms to microscopic slides, was shown to eliminate the antigenic reactivity of Chlamydia pneumoniae elementary bodies with fluorescent antibodies. Acetone...
Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test.
Wang, S P, Kuo, C C, Grayston, J T
Chlamydia trachomatis organisms grown in HeLa 229 cell cultures were purified and formalinized for use in the micro-immunofluorescence test. As test antigens, they were stable when stored unfrozen at...
Wang, S P, Grayston, J T, Alexander, E R, Holmes, K K
A simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens has been devised as a screening test for antibody in human sera. The test differs...
A mouse model of Chlamydia pneumoniae strain TWAR pneumonitis.
Yang, Z P, Kuo, C C, Grayston, J T
Chlamydia pneumoniae is a common cause of acute respiratory infections in humans. We evaluated mice as experimental animals for C. pneumoniae. Intranasal inoculation of Swiss Webster mice with C....
Campbell, L A, Kuo, C C, Thissen, R W, Grayston, J T
Chlamydia sp. strain TWAR is a unique Chlamydia sp. that causes acute respiratory disease. A gene bank consisting of TWAR isolate AR-39 DNA in pUC19 was screened with anti-AR-39 rabbit immune sera....
Comparative infectivity of trachoma organisms in HeLa 229 cells and egg cultures.
Kuo, C C, Wang, S P, Grayston, J T
Thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in HeLa 229 cells pretreated with diethylaminoethyl-dextran to determine the relative...
Characterization of the Group Antigen of Chlamydia trachomatis
Dhir, Sanittar P., Kenny, George E., Grayston, J. T.
A lipid complement-fixing group antigen is shared by all chlamydiae including trachoma organisms. A water-soluble polysaccharide antigen was obtained by alkali saponification from partially purified...
Jackson, L. A., Campbell, L. A., Schmidt, R. A., Kuo, C. C., Cappuccio, A. L., Lee, M. J., ...
Chlamydia pneumoniae has been detected in atherosclerotic plaque, raising the question of whether this detection is specific to atheromatous tissue. To evaluate this question, we tested...
Experimental rabbit models of Chlamydia pneumoniae infection.
Moazed, T. C., Kuo, C., Patton, D. L., Grayston, J. T., Campbell, L. A.
Chlamydia pneumoniae (TWAR), a common cause of acute respiratory disease in humans, has recently been associated with coronary and aortic atherosclerosis. In this study, we evaluated rabbit models of...
Chlamydia pneumoniae and cardiovascular disease.
Campbell, L. A., Kuo, C. C., Grayston, J. T.
Chlamydia pneumoniae is a ubiquitous pathogen that causes acute respiratory disease. The spectrum of C. pneumoniae infection has been extended to atherosclerosis and its clinical manifestations....
Jackson, L. A., Smith, N. L., Heckbert, S. R., Grayston, J. T., Siscovick, D. S., Psaty, B. M.
To evaluate the association of prior treatment with antibiotics active against Chlamydia pneumoniae with the risk for incident myocardial infarction, we conducted a population-based case-control...
Trachoma virus isolation studies on Taiwan*
Woolridge, R. L., Wang, S. P., Grayston, J. T.
Studies on trachoma have been carried out on Taiwan since 1958 by the US Naval Medical Research Unit No. 2. Routine methods for the collection of virus specimens, storage and processing of successful...