Singer, B, Antoccia, A, Basu, A K, Dosanjh, M K, Fraenkel-Conrat, H, Gallagher, P E, ...
We previously described a protein, isolated from human tissues and cells, that bound to a defined double-stranded oligonucleotide containing a single site-specifically placed 1,N6-ethenoadenine. It...
Singer, B, Spengler, S J, Fraenkel-Conrat, H, Kuśmierek, J T
In a previous paper, we reported that O4-methyl dTTP can be incorporated into poly(dA-dT) in place of thymidine without distortion of the helical structure, but on replication it could behave as...
In non-aqueous solution, diazomethane and diazoethane react with the O2, O4 and N-3 sites of uridine, thymidine, 1-methyluracil and 1-methylthymine. Diazoethane has a higher affinity for alkylating...
Singer, B, Sági, J, Kuśmierek, J T
O2-and O4-alkyldeoxythymidine are among the four O-alkyl base-modified derivatives produced by the reaction of N-nitroso alkylating agents with nucleic acids in vitro and in vivo. We find that both...
Preparation and template activities of polynucleotides containing O2- and O4-alkyluridine.
Singer, B, Fraenkel-Conrat, H, Kuśmierek, J T
O2-Ethyl-UDP and O4-methyl-UDP have been prepared and copolymerized in various proportions with UDP or CDP, using polynucleotide phosphorylase. The copolymers were used as templates for DNA-dependent...
In vitro discrimination of replicases acting on carcinogen-modified polynucleotide templates
Singer, B., Kuśmierek, J. T., Fraenkel-Conrat, H.
Three different poly(dC)s with modifications that block the N-3 of deoxycytidine were used as templates for polymer synthesis by Escherichia coli DNA polymerase I (EC 2.7.7.7). In contrast to...
Singer, B, Antoccia, A, Basu, A K, Dosanjh, M K, Fraenkel-Conrat, H, Gallagher, P E, ...
We previously described a protein, isolated from human tissues and cells, that bound to a defined double-stranded oligonucleotide containing a single site-specifically placed 1,N6-ethenoadenine. It...
Singer, B, Spengler, S J, Fraenkel-Conrat, H, Kuśmierek, J T
In a previous paper, we reported that O4-methyl dTTP can be incorporated into poly(dA-dT) in place of thymidine without distortion of the helical structure, but on replication it could behave as...
In non-aqueous solution, diazomethane and diazoethane react with the O2, O4 and N-3 sites of uridine, thymidine, 1-methyluracil and 1-methylthymine. Diazoethane has a higher affinity for alkylating...
Singer, B, Sági, J, Kuśmierek, J T
O2-and O4-alkyldeoxythymidine are among the four O-alkyl base-modified derivatives produced by the reaction of N-nitroso alkylating agents with nucleic acids in vitro and in vivo. We find that both...
Preparation and template activities of polynucleotides containing O2- and O4-alkyluridine.
Singer, B, Fraenkel-Conrat, H, Kuśmierek, J T
O2-Ethyl-UDP and O4-methyl-UDP have been prepared and copolymerized in various proportions with UDP or CDP, using polynucleotide phosphorylase. The copolymers were used as templates for DNA-dependent...
In vitro discrimination of replicases acting on carcinogen-modified polynucleotide templates
Singer, B., Kuśmierek, J. T., Fraenkel-Conrat, H.
Three different poly(dC)s with modifications that block the N-3 of deoxycytidine were used as templates for polymer synthesis by Escherichia coli DNA polymerase I (EC 2.7.7.7). In contrast to...