Sotoca Covaleda, A.M., Vervoort, J., Strom, A., Gustafsson, J.A., ...
Breast cancer cells show overexpression of estrogen receptor (ER) relative to ERß compared to normal breast tissues. This observation has lead to the hypothesis that ERß may modulate the...
S. J. Abspoel, J. Vervoort, J. E. Rooda
Multipoint linear approximations for stochastic chance constrained
Usta, M., Wortelboer, H.M., Vervoort, J., Boersma, M.G., ...
Curcumin, an ¿,ß-unsaturated carbonyl compound, reacts with glutathione, leading to the formation of two monoglutathionyl curcumin conjugates. In the present study, the structures of both...
Fluorene transformation by bacteria of the genus Rhodococcus (2003)
Finkelstein, Z.I., Baskunov, B.P., Golovlev, E.L., Vervoort, J., Baboshin, M.A., ...
Of the four investigated Rhodococcus strains (R. rhodochrous172, R. opacus 4a and 557, and R. rhodnii 135), the first three strains were found to be able to completely transform fluorene when it was...
Awad, H.M., Boersma, M.G., Boeren, S., Vervoort, J.
In the present study, the formation of glutathionyl adducts from a series of 3',4'-dihydroxy flavonoid o-quinone/p-quinone methides was investigated with special emphasis on the regioselectivity of...
A new His-tagged based immobilization method for the reconstitution of apoflavoproteins (2002)
Hefti, M.H., Milder, F.J., Boeren, S., Vervoort, J.
The NifL PAS domain from Azotobacter vinelandii is a flavoprotein with FAD as the prosthetic group. Here we describe a novel immobilization procedure for the large-scale preparation of apo NifL PAS...
Prenafeta-Boldú, F.X., Vervoort, J., Grotenhuis, J.T.C.
The soil fungus Cladophialophora sp. strain T1 (= ATCC MYA-2335) was capable of growth on a model water-soluble fraction of gasoline that contained all six BTEX components (benzene, toluene,...
Isolation and characterization of a microperoxidase-8 with a modified histidine axial ligand (2002)
Primus, J.L., Boeren, S., Nielen, M.W.F., Vervoort, J., Banci, L.
Microperoxidase-8, Fe(III)MP-8, the heme octapeptide obtained by horse heart cytochrome c digestion, was studied in the presence of H 2O 2. A modified form of the catalyst was isolated by HPLC and...
Rivas, S., Mucyn, T., Vervoort, J., Jones, J.D.
Cf-9 proteins discriminate between two Cladosporium-encoded avirulence determinants, Avr4 and Avr9. Comparative studies between Cf-4 and Cf-9 are thus of particular interest. To investigate Cf-4...
Awad, H.M., Boersma, M.G., Boeren, S., ...
Formation of quercetin quinone/quinone methide metabolites, reflected by formation of the glutathionyl quercetin adducts as authentic metabolites, was investigated in an in vitro cell model (B16F-10...
Travkin, V., Baskunov, B.P., Golovlev, E.L., Boersma, M.G., Boeren, S., Vervoort, J., ...
In this paper we report the isolation and characterization of an anaerobic enrichment culture as well as of a Rhodococcus sp. strain 2 capable of degrading 3,4-dihaloanilines under nitrate reducing...
Ridder, L., Vervoort, J., Mullholland, A.J.
Glutathione S-transferases (GSTs) play an important role in the detoxification of xenobiotics in mammals. They catalyze the conjugation of glutathione to a wide range of electrophilic compounds....
19F NMR metabolomics for the elucidation of microbial degradation pathways of fluorophenols (2001)
Boersma, M.G., Solyanikova, I.P., Vervoort, J., Golovleva, L.A.
Of all NMR-observable isotopes 19F is the one most convenient for studies on the biodegradation of environmental pollutants and especially for fast initial metabolic screening of newly isolated...
Structure activity study on the quinone/quinone methide chemistry of flavonoids (2001)
Awad, H.M., Boersma, M.G., Boeren, S., Vervoort, J.
A structure-activity study on the quinone/quinone methide chemistry of a series of 3',4'-dihydroxyflavonoids was performed. Using the glutathione trapping method followed by HPLC, 1H NMR, MALDI-TOF,...
Transformation of the insecticide teflubenzuron by microorganisms (2001)
Finkelstein, Z.I., Baskunov, B.P., Boersma, M.G., Vervoort, J., Golovleva, L.A.
Transformation of teflubenzuron, the active component in the insecticide commercialized as Nomolt, by soil microorganisms was studied. It was shown that microorganisms, belonging to Bacillus,...
19F NMR metabolomics for the elucidation of microbial degradation pathways of fluorophenols (2000)
Boersma, M.G., Solyanikova, I., Vervoort, J., Golovleva, L.A.
In vivo or in situ nuclear magnetic resonance (NMR) offers a powerful tool to study the degradation of xenobiotics by microorganisms. Most studies reported are based on the use of heteronuclei, and...
Peroxidase-catalyzed formation of quercetin quinone methide-glutathione adducts (2000)
Awad, H.M., Boersma, M.G., Vervoort, J.
The oxidation of quercetin by horseradish peroxidase/H2O2 was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the...
Ridder, L., Mulholland, A.J., Vervoort, J.
A combined quantum mechanical and molecular mechanical (QM/MM) method (AM1/CHARMM) was used to investigate the mechanism of the aromatic hydroxylation of phenol by a flavin dependent phenol...
Ridder, L., Vervoort, J., Palfrey, B.
The simulation of enzymatic reactions, using computer models, is becoming a powerful tool in the most fundamental challenge in biochemistry: to relate the catalytic activity of enzymes to their...
Boersma, M.G., Vervoort, J., Szymusiak, H., Lemanska, K., Tyrakowska, B., Cenas, N., ...
The chemical reactivity, isomerization, and glutathione conjugation of quercetin o-quinone were investigated. Tyrosinase was used to generate the unstable quercetin o-quinone derivative which could...
Finkelstein, Z.I., Baskunov, B.P., Boersma, M.G., Vervoort, J., Golovlev, E.L., ...
The transformation of monofluorophenols by whole cells of Rhodococcus opacus 1cp was investigated, with special emphasis on the nature of hydroxylated intermediates formed. Thin-layer chromatography,...
S. J. Abspoel, J. Vervoort, J. E. Rooda
linear approximations for stochastic chance constrained optimization problems with integer design variables
The TEAC antioxidant activity of 4-hydroxybenzoates (1999)
Tyrakowska, B., Szymusiak, H., Boeren, S., Boersma, M.G., Lemanska, J., ...
Preferential oxidative dehalogenetion upon conversion of 2-halophenols by Rh.opacus 1G (1999)
Bondar, V.S., Boersma, M.G., Finkelstein, Z.I., Golovlev, E.L., Baskunov, B.P., ...
Claassens, M.M.J., Boersma, M.G., Vervoort, J.
For the first time saturating overall kcat values for horseradish peroxidase (HRP) catalysed conversion of phenols and anilines are described. These kcat values correlate quantitatively with...
Ridder, L., Mulholland, A., Vervoort, J.
The reaction pathway for the aromatic 3-hydroxylation of p-hydroxybenzoate by the reactive C4a-hydroperoxyflavin cofactor intermediate in p-hydroxybenzoate hydroxylase (PHBH) has been investigated by...
GSTP1-1 stereospecifically catalyzes glutathione conjugation of ethacrynic acid. (1998)
Using 1H NMR two diastereoisomers of the ethacrynic acid glutathione conjugate (EASG) as well as ethacrynic acid (EA) could be distinguished and quantified individually. Chemically prepared EASG...
19F NMR study on the biodegradation of fluorophenols by various Rhodococcus species (1998)
Bondar, V., Boersma, M.G., Golovleva, E.L., Vervoort, J., Finkelstein, Z.I., ...
Vervoort, J., Berg, A., Vossen, P.
The secondary structure and global fold of the AVR9 elicitor protein of Cladosporium fulvum has been determined by 2D NMR and distance-geometry protocols. The protein consists of three anti-parallel...
Veeger, C., Vervoort, J., Modi, S., ...
Moonen, M.J.H., Wobbes, T., Vervoort, J., ...
Cadmium-induced luminescence of recombinant photoprotein obelin. (1995)
Bondar, V.S., Sergeev, A.G., Illarionov, B.A., Vervoort, J., Hagen, W.R.
Honée, G., Cozijnsen, T.J., Laugé, R., ...
Eppink, M.H.M., Veeger, C., ...
Photoinduced charge separation by porphyrins immobilized in microemulsion interfaces. (1993)
Verhaert, R.M.D., Roeterdink, F., Vervoort, J., Hilhorst, R.
A comparative NMR study on the interaction of FMN with apoflavodoxins (1985)
Vervoort, J., Muller, F., Mayhew, S.G., Legall, J., Bacher, A.
A subanosecond resolving spectrofluorimeter for the analyses of protein fluorescence kinetics (1983)
Coherent anti-stokes raman spectra of isoalloxazines (1983)
Muller, F., Vervoort, J., Lee, J., Horowitz, M., Carreira, L.A.
Prenafeta-Boldú, F. X., Vervoort, J., Grotenhuis, J. T. C., Van Groenestijn, J. W.
The soil fungus Cladophialophora sp. strain T1 (= ATCC MYA-2335) was capable of growth on a model water-soluble fraction of gasoline that contained all six BTEX components (benzene, toluene,...
Eppink, M H, Boeren, S A, Vervoort, J, Van Berkel, W J
A novel flavoprotein monooxygenase, 4-hydroxybenzoate 1-hydroxylase (decarboxylating), from Candida parapsilosis CBS604 was purified to apparent homogeneity. The enzyme is induced when the yeast is...