Scientific Commons: J. W. Erickson

Plasmodium falciparum is the major causative agent of malaria, a disease of worldwide importance. Resistance to current drugs such as chloroquine and mefloquine is spreading at an alarming rate, and...

Selection of multiple human immunodeficiency virus type 1 variants that encode viral proteases with decreased sensitivity to an inhibitor of the viral protease.

Kaplan, A H, Michael, S F, Wehbie, R S, Knigge, M F, Paul, D A, Everitt, L, ...

Inhibitors of the human immunodeficiency virus type 1 (HIV-1) protease represent a promising addition to the available agents used to inhibit virus replication in a therapeutic setting. HIV-1 is...

Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein.

Sakaguchi, K, Zambrano, N, Baldwin, E T, Shapiro, B A, Erickson, J W, Omichinski, J G, ...

The nucleocapsid (NC) protein NCp7 of human immunodeficiency virus type 1 (HIV-1) is important for encapsidation of the virus genome, RNA dimerization, and primer tRNA annealing in vitro. Here we...

Crystal structures of native and inhibited forms of human cathepsin D: implications for lysosomal targeting and drug design.

Baldwin, E T, Bhat, T N, Gulnik, S, Hosur, M V, Sowder, R C, Cachau, R E, ...

Cathepsin D (EC 3.4.23.5) is a lysosomal protease suspected to play important roles in protein catabolism, antigen processing, degenerative diseases, and breast cancer progression. Determination of...

Identification and characterization of an outer membrane protein, OmpX, in Escherichia coli that is homologous to a family of outer membrane proteins including Ail of Yersinia enterocolitica.

Mecsas, J, Welch, R, Erickson, J W, Gross, C A

We previously reported that a region of the Escherichia coli chromosome at 18 min increased E sigma E activity when cloned in multicopy (J. Mecsas, P. E. Rouviere, J. W. Erickson, T. J. Donohue, and...

Selection and analysis of human immunodeficiency virus type 1 variants with increased resistance to ABT-538, a novel protease inhibitor.

Markowitz, M, Mo, H, Kempf, D J, Norbeck, D W, Bhat, T N, Erickson, J W, ...

Inhibitors of the human immunodeficiency virus protease represent a promising new class of antiretroviral drugs for the treatment of AIDS. We now report the in vitro selection of viral variants with...

In vivo sequence diversity of the protease of human immunodeficiency virus type 1: presence of protease inhibitor-resistant variants in untreated subjects.

Lech, W J, Wang, G, Yang, Y L, Chee, Y, Dorman, K, McCrae, D, ...

We have evaluated the sequence diversity of the protease human immunodeficiency virus type 1 in vivo. Our analysis of 246 protease coding domain sequences obtained from 12 subjects indicates that...

Transcriptional regulation of the heat shock regulatory gene rpoH in Escherichia coli: involvement of a novel catabolite-sensitive promoter.

Nagai, H, Yano, R, Erickson, J W, Yura, T

A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ,...

Deletion and insertion mutations in the rpoH gene of Escherichia coli that produce functional sigma 32.

Calendar, R, Erickson, J W, Halling, C, Nolte, A

Escherichia coli K-12 strain 285c contains a short deletion mutation in rpoD, the gene encoding the sigma 70 subunit of RNA polymerase. The sigma 70 protein encoded by this allele (rpoD285) unstable,...

Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.

Zhou, Y N, Kusukawa, N, Erickson, J W, Gross, C A, Yura, T

The product of the Escherichia coli rpoH (htpR) gene, sigma 32, is required for heat-inducible transcription of the heat shock genes. Previous studies on the role of sigma 32 in growth at low...

Characterization of human immunodeficiency virus type 1 variants with increased resistance to a C2-symmetric protease inhibitor.

Ho, D D, Toyoshima, T, Mo, H, Kempf, D J, Norbeck, D, Chen, C M, ...

Inhibitors of the human immunodeficiency virus type 1 protease represent a promising class of antiviral drugs for the treatment of AIDS, and several are now in clinical trials. Here, we report the in...

A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.

Singer, M, Baker, T A, Schnitzler, G, Deischel, S M, Goel, M, Dove, W, ...

We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most...

The Drosophila melanogaster sex determination gene sisA is required in yolk nuclei for midgut formation.

Walker, J J, Lee, K K, Desai, R N, Erickson, J W

During sex determination, the sisterlessA (sisA) gene functions as one of four X:A numerator elements that set the alternative male or female regulatory states of the switch gene Sex-lethal. In...

Structure and inhibition of plasmepsin II, a hemoglobin-degrading enzyme from Plasmodium falciparum.

Silva, A M, Lee, A Y, Gulnik, S V, Maier, P, Collins, J, Bhat, T N, ...

Plasmodium falciparum is the major causative agent of malaria, a disease of worldwide importance. Resistance to current drugs such as chloroquine and mefloquine is spreading at an alarming rate, and...

Selection of multiple human immunodeficiency virus type 1 variants that encode viral proteases with decreased sensitivity to an inhibitor of the viral protease.

Kaplan, A H, Michael, S F, Wehbie, R S, Knigge, M F, Paul, D A, Everitt, L, ...

Inhibitors of the human immunodeficiency virus type 1 (HIV-1) protease represent a promising addition to the available agents used to inhibit virus replication in a therapeutic setting. HIV-1 is...

Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein.

Sakaguchi, K, Zambrano, N, Baldwin, E T, Shapiro, B A, Erickson, J W, Omichinski, J G, ...

The nucleocapsid (NC) protein NCp7 of human immunodeficiency virus type 1 (HIV-1) is important for encapsidation of the virus genome, RNA dimerization, and primer tRNA annealing in vitro. Here we...

Crystal structures of native and inhibited forms of human cathepsin D: implications for lysosomal targeting and drug design.

Baldwin, E T, Bhat, T N, Gulnik, S, Hosur, M V, Sowder, R C, Cachau, R E, ...

Cathepsin D (EC 3.4.23.5) is a lysosomal protease suspected to play important roles in protein catabolism, antigen processing, degenerative diseases, and breast cancer progression. Determination of...

Identification and characterization of an outer membrane protein, OmpX, in Escherichia coli that is homologous to a family of outer membrane proteins including Ail of Yersinia enterocolitica.

Mecsas, J, Welch, R, Erickson, J W, Gross, C A

We previously reported that a region of the Escherichia coli chromosome at 18 min increased E sigma E activity when cloned in multicopy (J. Mecsas, P. E. Rouviere, J. W. Erickson, T. J. Donohue, and...

Selection and analysis of human immunodeficiency virus type 1 variants with increased resistance to ABT-538, a novel protease inhibitor.

Markowitz, M, Mo, H, Kempf, D J, Norbeck, D W, Bhat, T N, Erickson, J W, ...

Inhibitors of the human immunodeficiency virus protease represent a promising new class of antiretroviral drugs for the treatment of AIDS. We now report the in vitro selection of viral variants with...

In vivo sequence diversity of the protease of human immunodeficiency virus type 1: presence of protease inhibitor-resistant variants in untreated subjects.

Lech, W J, Wang, G, Yang, Y L, Chee, Y, Dorman, K, McCrae, D, ...

We have evaluated the sequence diversity of the protease human immunodeficiency virus type 1 in vivo. Our analysis of 246 protease coding domain sequences obtained from 12 subjects indicates that...

Transcriptional regulation of the heat shock regulatory gene rpoH in Escherichia coli: involvement of a novel catabolite-sensitive promoter.

Nagai, H, Yano, R, Erickson, J W, Yura, T

A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ,...

Deletion and insertion mutations in the rpoH gene of Escherichia coli that produce functional sigma 32.

Calendar, R, Erickson, J W, Halling, C, Nolte, A

Escherichia coli K-12 strain 285c contains a short deletion mutation in rpoD, the gene encoding the sigma 70 subunit of RNA polymerase. The sigma 70 protein encoded by this allele (rpoD285) unstable,...

Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.

Zhou, Y N, Kusukawa, N, Erickson, J W, Gross, C A, Yura, T

The product of the Escherichia coli rpoH (htpR) gene, sigma 32, is required for heat-inducible transcription of the heat shock genes. Previous studies on the role of sigma 32 in growth at low...

Characterization of human immunodeficiency virus type 1 variants with increased resistance to a C2-symmetric protease inhibitor.

Ho, D D, Toyoshima, T, Mo, H, Kempf, D J, Norbeck, D, Chen, C M, ...

Inhibitors of the human immunodeficiency virus type 1 protease represent a promising class of antiviral drugs for the treatment of AIDS, and several are now in clinical trials. Here, we report the in...

A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.

Singer, M, Baker, T A, Schnitzler, G, Deischel, S M, Goel, M, Dove, W, ...

We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most...

The Drosophila melanogaster sex determination gene sisA is required in yolk nuclei for midgut formation.

Walker, J J, Lee, K K, Desai, R N, Erickson, J W

During sex determination, the sisterlessA (sisA) gene functions as one of four X:A numerator elements that set the alternative male or female regulatory states of the switch gene Sex-lethal. In...

Real-time DNA binding measurements of the ETS1 recombinant oncoproteins reveal significant kinetic differences between the p42 and p51 isoforms.

Fisher, R. J., Fivash, M., Casas-Finet, J., Erickson, J. W., Kondoh, A., Bladen, S. V., ...

The sequence-specific DNA binding of recombinant p42 and p51 ETS1 oncoprotein was examined quantitatively to determine whether the loss of the Exon VII phosphorylation domain in p42 ETS1 or the...

Structure-based subsite specificity mapping of human cathepsin D using statine-based inhibitors.

Majer, P., Collins, J. R., Gulnik, S. V., Erickson, J. W.

Human cathepsin D is a lysosomal aspartic protease that has been implicated in breast cancer metastasis and Alzheimer's disease. Based on a crystal structure of a human cathepsin D-pepstatin A...

Molecular mechanisms of resistance: free energy calculations of mutation effects on inhibitor binding to HIV-1 protease.

Rick, S. W., Topol, I. A., Erickson, J. W., Burt, S. K.

The changes in the inhibitor binding constants due to the mutation of isoleucine to valine at position 84 of HIV-1 protease are calculated using molecular dynamics simulations. The calculations are...

Real-time measurements of dark substrate catalysis.

Xie, D., Suvorov, L., Erickson, J. W., Gulnik, A. S.

We have developed a novel procedure to monitor the real-time cleavage of natural unmodified peptides (dark substrates). In the competition-based assay, the initial cleavage rate of a fluorogenic...

Drug resistance mutations can effect dimer stability of HIV-1 protease at neutral pH.

Xie, D., Gulnik, S., Gustchina, E., Yu, B., Shao, W., Qoronfleh, W., ...

The monomer-dimer equilibrium for the human immunodeficiency virus type 1 (HIV-1) protease has been investigated under physiological conditions. Dimer dissociation at pH 7.0 was correlated with a...

J. W. Erickson

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