Plasmid Detection in Legionella pneumophila and Legionella-like Organisms. (2002)
Mikesell,Perry, Ezzell,J. W., Knudson,Gregory B.
Five strains of bacteria, designated Legionella-like organisms, and one strain of Legionella pneumophila were examined for the presence of extrachromosomal deoxyribonucleic acid. Cryptic plasmids...
Plasmid Isolation in Legionella pneumophila and Legionella-like Organisms. (2002)
Mikesell,Perry, Ezzell,J. W., Knudson,Gregory B.
Agarose gel electrophoresis was employed to screen nine strains of Legionella-like bacteria and one strain of Legionella pneumophila for the presence of extrachromosomal deoxyribonucleic acid....
Production and Validation of the Use of Gamma Phage for Identification of Bacillus anthracis (1998)
Abshire, T. G., Brown, J. E., Ezzell, J. W.
Gamma phage specifically lyses vegetative cells of Bacillus anthracis and serves as part of the basis for identification of isolates from agar cultures. We report our study to standardize gamma phage...
Farchaus, J W, Ribot, W J, Downs, M B, Ezzell, J W
Many prokaryotic organisms possess surface layer (S-layer) proteins that are components of the outermost cell envelope. With immunogold labeling, it was demonstrated that the protein extractable...
Cyclic AMP regulation of the hexose phosphate transport system in Escherichia coli.
Synthesis of the hexosephosphate transport system in Escherichia coli required the cyclic AMP-receptor protein regulatory complex. The apparent Km value for hexosephosphate activity was affected by...
A cyclic adenosine 3',5'-monophosphate (cAMP)-deficient mutant strain of Escherichia coli K-12 was studied to determine the effect this cyclic nucleotide has on the overall growth and metabolism of...
Immunological analysis of cell-associated antigens of Bacillus anthracis.
Sera from Hartley guinea pigs vaccinated with a veterinary live spore anthrax vaccine were compared with sera from guinea pigs vaccinated with the human anthrax vaccine, which consists of aluminum...
Immunization studies with attenuated strains of Bacillus anthracis.
Ivins, B E, Ezzell, J W, Jemski, J, Hedlund, K W, Ristroph, J D, Leppla, S H
Live, attenuated strains of Bacillus anthracis lacking either the capsule plasmid pXO2, the toxin plasmid pXO1, or both were tested for their efficacy as vaccines against intravenous challenge with...
Ezzell, J W, Ivins, B E, Leppla, S H
The kinetics of Bacillus anthracis toxin production in culture and its lethal activity in rats, mice, and guinea pigs were investigated. Lethal toxin activity was produced in vitro throughout...
Use of immunoelectron microscopy to demonstrate Francisella tularensis.
Geisbert, T W, Jahrling, P B, Ezzell, J W
Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct...
Ezzell, J W, Abshire, T G, Little, S F, Lidgerding, B C, Brown, C
Guanidine extracts of crude Bacillus anthracis cell wall were used to vaccinate BALB/c mice and to develop monoclonal antibody (MAb) to vegetative cell surface antigens. Two hybridomas selected...
Differentiation of Bacillus anthracis and other Bacillus species by lectins.
Cole, H B, Ezzell, J W, Keller, K F, Doyle, R J
Bacillus anthracis was agglutinated by several lectins, including those from Griffonia simplicifolia, Glycine max, Abrus precatorius, and Ricinus communis. Some strains of Bacillus cereus var....
Glycosidase activities of Bacillus anthracis.
Sadler, D F, Ezzell, J W, Keller, K F, Doyle, R J
Bacillus anthracis could be distinguished from the taxonomically related species B. cereus, B. mycoides, and B. thuringiensis by a comparison of glycosidase activities. All the bacilli tested...
Isolation of plasmids in Legionella pneumophila and Legionella-like organisms.
Mikesell, P, Ezzell, J W, Knudson, G B
Agarose gel electrophoresis was employed to screen nine strains of Legionella-like bacteria and one strain of Legionella pneumophila for the presence of extrachromosomal deoxyribonucleic acid....
Production and Validation of the Use of Gamma Phage for Identification of Bacillus anthracis
Abshire, T. G., Brown, J. E., Ezzell, J. W.
Gamma phage specifically lyses vegetative cells of Bacillus anthracis and serves as part of the basis for identification of isolates from agar cultures. We report our study to standardize gamma phage...
Farchaus, J W, Ribot, W J, Downs, M B, Ezzell, J W
Many prokaryotic organisms possess surface layer (S-layer) proteins that are components of the outermost cell envelope. With immunogold labeling, it was demonstrated that the protein extractable...
Cyclic AMP regulation of the hexose phosphate transport system in Escherichia coli.
Synthesis of the hexosephosphate transport system in Escherichia coli required the cyclic AMP-receptor protein regulatory complex. The apparent Km value for hexosephosphate activity was affected by...
A cyclic adenosine 3',5'-monophosphate (cAMP)-deficient mutant strain of Escherichia coli K-12 was studied to determine the effect this cyclic nucleotide has on the overall growth and metabolism of...
Immunological analysis of cell-associated antigens of Bacillus anthracis.
Sera from Hartley guinea pigs vaccinated with a veterinary live spore anthrax vaccine were compared with sera from guinea pigs vaccinated with the human anthrax vaccine, which consists of aluminum...
Immunization studies with attenuated strains of Bacillus anthracis.
Ivins, B E, Ezzell, J W, Jemski, J, Hedlund, K W, Ristroph, J D, Leppla, S H
Live, attenuated strains of Bacillus anthracis lacking either the capsule plasmid pXO2, the toxin plasmid pXO1, or both were tested for their efficacy as vaccines against intravenous challenge with...
Ezzell, J W, Ivins, B E, Leppla, S H
The kinetics of Bacillus anthracis toxin production in culture and its lethal activity in rats, mice, and guinea pigs were investigated. Lethal toxin activity was produced in vitro throughout...
Use of immunoelectron microscopy to demonstrate Francisella tularensis.
Geisbert, T W, Jahrling, P B, Ezzell, J W
Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct...
Ezzell, J W, Abshire, T G, Little, S F, Lidgerding, B C, Brown, C
Guanidine extracts of crude Bacillus anthracis cell wall were used to vaccinate BALB/c mice and to develop monoclonal antibody (MAb) to vegetative cell surface antigens. Two hybridomas selected...
Differentiation of Bacillus anthracis and other Bacillus species by lectins.
Cole, H B, Ezzell, J W, Keller, K F, Doyle, R J
Bacillus anthracis was agglutinated by several lectins, including those from Griffonia simplicifolia, Glycine max, Abrus precatorius, and Ricinus communis. Some strains of Bacillus cereus var....
Glycosidase activities of Bacillus anthracis.
Sadler, D F, Ezzell, J W, Keller, K F, Doyle, R J
Bacillus anthracis could be distinguished from the taxonomically related species B. cereus, B. mycoides, and B. thuringiensis by a comparison of glycosidase activities. All the bacilli tested...
Isolation of plasmids in Legionella pneumophila and Legionella-like organisms.
Mikesell, P, Ezzell, J W, Knudson, G B
Agarose gel electrophoresis was employed to screen nine strains of Legionella-like bacteria and one strain of Legionella pneumophila for the presence of extrachromosomal deoxyribonucleic acid....
Production and Validation of the Use of Gamma Phage for Identification of Bacillus anthracis
Abshire, T. G., Brown, J. E., Ezzell, J. W.
Gamma phage specifically lyses vegetative cells of Bacillus anthracis and serves as part of the basis for identification of isolates from agar cultures. We report our study to standardize gamma phage...
Association of Bacillus anthracis Capsule with Lethal Toxin during Experimental Infectionâ–ż
Ezzell, J. W., Abshire, T. G., Panchal, R., Chabot, D., Bavari, S., Leffel, E. K., ...
Bacillus anthracis lethal toxin (LT) was characterized in plasma from infected African Green monkeys, rabbits, and guinea pigs. In all cases, during the terminal phase of infection only the...