J. Widengren

Triplet imaging contrast by modulated excitation (2008)

Geissbuehler, M., Spielmann, T., Vulliamy, R., Leutenegger, M., Märki, I., Sandén, T., ...

A novel imaging method employing the different kinetics of the energetic states of fluorescent markers is presented, where the contrast depends on the triplet kinetics. An attractive feature of...

Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy (2007)

Widengren, J., Chmyrov, A., Eggeling, C., Loefdahl, P. A., Seidel, C.

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy (2007)

Hassler, K., Rigler, P., Blom, H., Rigler, R., Widengren, J., Lasser, T.

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on...

Analysis of Photobleaching in Single-Molecule Multicolor Excitation and Förster Resonance Energy Transfer Measurements. (2006)

Eggeling, C., Widengren, J., Brand, L., Schaffer, J., Felekyan, S., Seidel, C.

Dual-color total internal reflection fluorescence cross-correlation spectroscopy (2006)

Leutenegger, M., Blom, H., Widengren, J., Eggeling, C., Goesch, M., Leitgeb, R. A., ...

Dual-color total internal reflection fluorescence cross- correlation spectroscopy (2006)

Leutenegger, M., Blom, H., Widengren, J., Eggeling, C., Gösch, M., Leitgeb, R.A., ...

We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation...

Dual-color Total Internal Reflection Fluorescence Fluctuation Spectroscopy (2006)

Leutenegger, M., Blom, H., Widengren, J., Eggeling, C., Gösch, M., Lasser, T.

We developed a dual-color total internal reflection fluorescence (TIRF) system for single molecule imaging and fluorescence fluctuation spectroscopy (FFS). For a performance analysis, we measured a...

Dual-color Total Internal Reflection Fluorescence Fluctuation Spectroscopy (2006)

Leutenegger, M., Blom, H., Widengren, J., Eggeling, C., Gösch, M., Lasser, T.

We developed a dual-color total internal reflection fluorescence (TIRF) system for single molecule imaging and fluorescence fluctuation spectroscopy (FFS). For a performance analysis, we measured a...

Single-molecule fluorescence resonance energy transfer reveals a dynamic equilibrium between closed and open conformations of syntaxin 1 (2003)

Margittai, M., Widengren, J., Schweinberger, E., Schroder, G., Felekyan, S., Haustein, E., ...

Single molecule FRET reveals an equilibrium between a closed and an open conformation of syntaxin 1 (2002)

Margittai, M., Schweinberger, E., Widengren, J., Fasshauer, D., Jahn, R., Schroeder, G., ...

Fluorescence correlation spectroscopy of flavins and flavoenzymes: photochemical and photophysical aspects. (2001)

Widengren, J., Hink, M.A., Rigler, R.

Two new concepts to measure fluorescence resonance energy transfer via fluorescence correlation spectroscopy: theory and experimental realization. (2001)

Widengren, J., Schweinberger, E., Berger, S., Seidel, C.

Characterization of photo-induced isomerization and back-isomerization of the cyanine dye Cy5 by fluorescence correlation spectroscopy. (2000)

Widengren, J., Schwille, P.

Manipulation and characterization of photo-induced states of merocyanine 540 by fluorescence correlation spectroscopy. (2000)

Widengren, J., Seidel, C.

Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis. (1998)

Eggeling, C., Widengren, J., Rigler, R., Seidel, C.

Photostability of fluorescent dyes for single-molecule spectroscopy: Mechanisms and experimental methods for estimating photobleaching in aqueous solution. (1998)

Rettig, W., Strehmel, B., Schrader, S., Eggeling, C., Widengren, J., Rigler, R., ...

Photophysical characterization of the dye Cy-5 by fluorescence correlation spectroscopy (1997)

Widengren, J., Schwille, P., Rigler, R.

Single-molecule fluorescence resonance energy transfer reveals a dynamic equilibrium between closed and open conformations of syntaxin 1

Margittai, M., Widengren, J., Schweinberger, E., Schröder, G. F., Felekyan, S., Haustein, E., ...

Protein conformational transitions form the molecular basis of many cellular processes, such as signal transduction and membrane traffic. However, in many cases, little is known about their...

Single-molecule fluorescence resonance energy transfer reveals a dynamic equilibrium between closed and open conformations of syntaxin 1

Margittai, M., Widengren, J., Schweinberger, E., Schröder, G. F., Felekyan, S., Haustein, E., ...

Protein conformational transitions form the molecular basis of many cellular processes, such as signal transduction and membrane traffic. However, in many cases, little is known about their...