Emerging Roles As Voltage Sensors? (2008)
Phosphoinositide Phosphatases, Carolyn A. Worby, Jack E. Dixon
Phosphoinositide phosphatases belong to the protein tyrosine phosphatase (PTP) superfamily whose hallmark is a highly conserved active site motif, Cys-Xxx 5-Arg (CX 5R). Other members of this...
A role for AGL ubiquitination in the glycogen storage disorders of Lafora and Cori's disease (2007)
Cheng, Alan, Zhang, Mei, Gentry, Matthew S., Worby, Carolyn A., Dixon, Jack E., Saltiel, Alan R.
Cori’s disease is a glycogen storage disorder characterized by a deficiency in the glycogen debranching enzyme, amylo-1,6-glucosidase,4-α-glucanotransferase (AGL). Here, we demonstrate that the...
The Sorcerer II Global Ocean Sampling Expedition: Expanding the Universe of Protein Families (2007)
Shibu Yooseph, Granger Sutton, Douglas B. Rusch, Aaron L. Halpern, Shannon J. Williamson, Karin Remington, ...
Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a...
The Sorcerer II global ocean sampling expedition: expanding the universe of protein families (2007)
Shibu Yooseph, Granger Sutton, Douglas B. Rusch, Aaron L. Halpern, Shannon J. Williamson, Karin Remington, ...
Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a...
Paulsen, Michelle T, Starks, Adrienne M, Derheimer, Frederick A, Hanasoge, Sheela, Li, Liwu, Dixon, Jack E, ...
Abstract Background The evolutionary conserved cyclin-dependent kinase phosphatase hCdc14A has been shown to play potential roles in the regulation of mitotic exit and in the centrosome duplication...
The Sorcerer II Global Ocean Sampling Expedition: Expanding the Universe of Protein Families (2006)
Yooseph, Shibu, Sutton, Granger, Rusch, Douglas B., Halpern, Aaron L., Williamson, Shannon J., Remington, Karin, ...
Protein tyrosine phosphatases in disease processes (1994)
Ninfa, Elizabeth G., Dixon, Jack E.
Given the importance of tyrosine phosphorylation of proteins in signalling pathways, it is perhaps not surprising that protein tyrosine phosphatases (PTPs) are involved in the pathogenesis of certain...
Chromosomal Localization of Four Human VH1-like Protein-Tyrosine Phosphatases (1994)
Martell, Karen J., Kwak, Seung P., Hakes, David J., Dixon, Jack E., Trent, Jeffrey M.
Four human protein-tyrosine phosphatase (PTPase) genes of the VH1-like subclass were cloned by low-stringency screening of a genomic library. These genes were localized to their respective...
Mauro, Laura J., Dixon, Jack E.
The transmembrane and intracellular protein tyrosine phosphatases (PTPs) play an essential role as signal transduction proteins involved in various cellular processes including division,...
Protein tyrosine phosphatases: characterization of extracellular and intracellular domains (1994)
Mourey, Robert J., Dixon, Jack E.
Protein tyrosine phosphatases (PTPs) play an important role in the regulation of cell growth and differentiation. With over 30 PTPs identified, the specific functions of these enzymes are now being...
Bacterial and viral protein tyrosine phosphatases (1993)
Guan, Kun-Liang, Dixon, Jack E.
Unrestricted protein tyrosine phosphatase (PTPase) activity may play a role in pathogenesis. For instance, the virulence determinant gene, yopH, of Yersinia pseudotuberculosis encodes a PTPase. The...
Walton, Kevin M., Martell, Karen J., Kwak, Seung P., Dixon, Jack E., Largent, Brian L.
Tyrosine phosphorylation plays a central role in the control of neuronal cell development and function. Yet, few neuronal protein tyrosine phosphatases (PTPs) have been identified. We examined rat...
Tissue-specific expression of the human neuropeptide Y gene in transgenic mice (1992)
Waldbieser, Geoffrey C., Minth, Carolyn D., Chrisman, C. Larry, Dixon, Jack E.
Neuropeptide Y (NPY) is the most abundant neuropeptide detected in the mammalian brain, and is found throughout the central and peripheral nervous systems. This peptide is a proposed regulator of...
Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase (1992)
Powell, Steven M., Zalkin, Howard, Dixon, Jack E.
Adenylosuccinate synthetase (AS) catalyzes the first committed step in the conversion of IMP to AMP. A cDNA was isolated from a human liver library which encodes a protein of 455 amino acids (Mr of...
New vectors for high level expression of recombinant proteins in bacteria (1992)
Hakes, David J., Dixon, Jack E.
A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase ([3.], Gene 67, 31-40). Expressed fusion proteins are...
Aimi, Junko, Qiu, Hong, Williams, John, Zalkin, Howard, Dixon, Jack E.
The trifunctional enzyme encoding glycinamide ribonucleotide synthetase (GARS)-aminolmidazole ribonucleotide synthetase(AIRS)-glycinamide ribonucleotide transformylase (GART) was cloned by functional...
Andrisani, Ourania M., Hayes, Timothy E., Roos, Bernard, Dixon, Jack E.
DNA sequences containing the 5′ flanking region of the rat somatoscatin gene were linked to the coding sequence of the bacterial chloramphenicol acetyl transferase gene. This recombinant plasmid is...
Taylor, Gregory S., Maehama, Tomohiko, Dixon, Jack E.
The lipid second messenger phosphatidylinositol 3-phosphate [PI(3)P] plays a crucial role in intracellular membrane trafficking. We report here that myotubularin, a protein tyrosine phosphatase...
Juris, Stephen J., Rudolph, Amy E., Huddler, Don, Orth, Kim, Dixon, Jack E.
The bacterial pathogens of the genus Yersinia deliver several virulence factors into target cells using a type III secretion system. We demonstrate that Yersinia protein kinase A (YpkA), an essential...
Clemens, James C., Worby, Carolyn A., Simonson-Leff, Nancy, Muda, Marco, Maehama, Tomohiko, Hemmings, Brian A., ...
We demonstrate the efficacy of double-stranded RNA-mediated interference (RNAi) of gene expression in generating “knock-out” phenotypes for specific proteins in several Drosophila cell lines. We...
Gottlin, Elizabeth B., Rudolph, Amy E., Zhao, Yi, Matthews, Harry R., Dixon, Jack E.
The phospholipase D (PLD) superfamily includes enzymes of phospholipid metabolism, nucleases, as well as ORFs of unknown function in viruses and pathogenic bacteria. These enzymes are characterized...
Ozes, Osman Nidai, Akca, Hakan, Mayo, Lindsey D., Gustin, Jason A., Maehama, Tomohiko, Dixon, Jack E., ...
Tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) by the insulin receptor permits this docking protein to interact with signaling proteins that promote insulin action. Serine...
Wishart, Matthew J., Dixon, Jack E.
Differentiation of spermatids into spermatozoa is regulated via phosphorylated RNA-binding proteins that modulate the expression of stage-specific mRNAs. We demonstrate that the phosphoserine,...
Kim, Soo-A, Vacratsis, Panayiotis O., Firestein, Ron, Cleary, Michael L., Dixon, Jack E.
The myotubularin (MTM) family constitutes one of the most highly conserved protein-tyrosine phosphatase subfamilies in eukaryotes. MTM1, the archetypal member of this family, is mutated in X-linked...
Membrane-binding and activation mechanism of PTEN
Das, Sudipto, Dixon, Jack E., Cho, Wonhwa
PTEN is a tumor suppressor that reverses the action of phosphoinositide 3-kinase by catalyzing the removal of the 3′ phosphate of phosphoinositides. Despite the critical role of PTEN in cell...
Shao, Feng, Vacratsis, Panayiotis O., Bao, Zhaoqin, Bowers, Katherine E., Fierke, Carol A., Dixon, Jack E.
The Gram-negative bacterial pathogen Yersinia delivers six effector proteins into the host cells to thwart the host innate immune response. One of the effectors, YopT, causes the disruption of the...
Zhu, Minfeng, Shao, Feng, Innes, Roger W., Dixon, Jack E., Xu, Zhaohui
AvrPphB is an avirulence (Avr) protein from the plant pathogen Pseudomonas syringae that can trigger a disease-resistance response in a number of host plants including Arabidopsis. AvrPphB belongs to...
Biologically Active Catecholamines Covalently Bound to Glass Beads
Venter, J. Craig, Dixon, Jack E., Maroko, Peter R., Kaplan, Nathan O.
Catecholamines bound covalently to glass beads have been found to have biological activity in several systems. Experimental evidence has been found that immobilized epinephrine and isoproterenol...
Immobilized Catecholamine and Cocaine Effects on Contractility of Cardiac Muscle
Venter, J. Craig, Ross, John, Dixon, Jack E., Mayer, Steven E., Kaplan, Nathan O.
Isoproterenol, norepinephrine, and epinephrine covalently bound to glass beads exert a positive inotropic effect on isometrically contracting papillary muscles from cats. Immobilized isoproterenol...
Kaplan, Nathan O., Everse, Johannes, Dixon, Jack E., Stolzenbach, Francis E., Lee, Chi-Yu, Lee, Ching-Lun T., ...
A number of different dehydrogenases have been shown to bind to Sepharose-bound N6-(6-aminohexyl)-AMP. These dehydrogenases can be specifically eluted by binary adducts of NAD+ or with cofactor...
Gentry, Matthew S., Worby, Carolyn A., Dixon, Jack E.
Lafora disease (LD) is a fatal form of progressive myoclonus epilepsy caused by recessive mutations in either a gene encoding a dual-specificity phosphatase, known as laforin, or a recently...
Muda, Marco, Worby, Carolyn A, Simonson-Leff, Nancy, Clemens, James C, Dixon, Jack E
Despite the wealth of information generated by genome-sequencing projects, the identification of in vivo substrates of specific protein kinases and phosphatases is hampered by the large number of...
Begley, Michael J., Taylor, Gregory S., Brock, Melissa A., Ghosh, Partho, Woods, Virgil L., Dixon, Jack E.
Myotubularins, a large family of catalytically active and inactive proteins, belong to a unique subgroup of protein tyrosine phosphatases that use inositol phospholipids, rather than phosphoproteins,...
Paulsen, Michelle T, Starks, Adrienne M, Derheimer, Frederick A, Hanasoge, Sheela, Li, Liwu, Dixon, Jack E, ...
Taylor, Gregory S., Maehama, Tomohiko, Dixon, Jack E.
The lipid second messenger phosphatidylinositol 3-phosphate [PI(3)P] plays a crucial role in intracellular membrane trafficking. We report here that myotubularin, a protein tyrosine phosphatase...
Juris, Stephen J., Rudolph, Amy E., Huddler, Don, Orth, Kim, Dixon, Jack E.
The bacterial pathogens of the genus Yersinia deliver several virulence factors into target cells using a type III secretion system. We demonstrate that Yersinia protein kinase A (YpkA), an essential...
Clemens, James C., Worby, Carolyn A., Simonson-Leff, Nancy, Muda, Marco, Maehama, Tomohiko, Hemmings, Brian A., ...
We demonstrate the efficacy of double-stranded RNA-mediated interference (RNAi) of gene expression in generating “knock-out” phenotypes for specific proteins in several Drosophila cell lines. We...
Gottlin, Elizabeth B., Rudolph, Amy E., Zhao, Yi, Matthews, Harry R., Dixon, Jack E.
The phospholipase D (PLD) superfamily includes enzymes of phospholipid metabolism, nucleases, as well as ORFs of unknown function in viruses and pathogenic bacteria. These enzymes are characterized...
Ozes, Osman Nidai, Akca, Hakan, Mayo, Lindsey D., Gustin, Jason A., Maehama, Tomohiko, Dixon, Jack E., ...
Tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) by the insulin receptor permits this docking protein to interact with signaling proteins that promote insulin action. Serine...
Wishart, Matthew J., Dixon, Jack E.
Differentiation of spermatids into spermatozoa is regulated via phosphorylated RNA-binding proteins that modulate the expression of stage-specific mRNAs. We demonstrate that the phosphoserine,...
Kim, Soo-A, Vacratsis, Panayiotis O., Firestein, Ron, Cleary, Michael L., Dixon, Jack E.
The myotubularin (MTM) family constitutes one of the most highly conserved protein-tyrosine phosphatase subfamilies in eukaryotes. MTM1, the archetypal member of this family, is mutated in X-linked...
Membrane-binding and activation mechanism of PTEN
Das, Sudipto, Dixon, Jack E., Cho, Wonhwa
PTEN is a tumor suppressor that reverses the action of phosphoinositide 3-kinase by catalyzing the removal of the 3′ phosphate of phosphoinositides. Despite the critical role of PTEN in cell...
Shao, Feng, Vacratsis, Panayiotis O., Bao, Zhaoqin, Bowers, Katherine E., Fierke, Carol A., Dixon, Jack E.
The Gram-negative bacterial pathogen Yersinia delivers six effector proteins into the host cells to thwart the host innate immune response. One of the effectors, YopT, causes the disruption of the...
Zhu, Minfeng, Shao, Feng, Innes, Roger W., Dixon, Jack E., Xu, Zhaohui
AvrPphB is an avirulence (Avr) protein from the plant pathogen Pseudomonas syringae that can trigger a disease-resistance response in a number of host plants including Arabidopsis. AvrPphB belongs to...
Biologically Active Catecholamines Covalently Bound to Glass Beads
Venter, J. Craig, Dixon, Jack E., Maroko, Peter R., Kaplan, Nathan O.
Catecholamines bound covalently to glass beads have been found to have biological activity in several systems. Experimental evidence has been found that immobilized epinephrine and isoproterenol...
Immobilized Catecholamine and Cocaine Effects on Contractility of Cardiac Muscle
Venter, J. Craig, Ross, John, Dixon, Jack E., Mayer, Steven E., Kaplan, Nathan O.
Isoproterenol, norepinephrine, and epinephrine covalently bound to glass beads exert a positive inotropic effect on isometrically contracting papillary muscles from cats. Immobilized isoproterenol...
Kaplan, Nathan O., Everse, Johannes, Dixon, Jack E., Stolzenbach, Francis E., Lee, Chi-Yu, Lee, Ching-Lun T., ...
A number of different dehydrogenases have been shown to bind to Sepharose-bound N6-(6-aminohexyl)-AMP. These dehydrogenases can be specifically eluted by binary adducts of NAD+ or with cofactor...
Gentry, Matthew S., Worby, Carolyn A., Dixon, Jack E.
Lafora disease (LD) is a fatal form of progressive myoclonus epilepsy caused by recessive mutations in either a gene encoding a dual-specificity phosphatase, known as laforin, or a recently...
Muda, Marco, Worby, Carolyn A, Simonson-Leff, Nancy, Clemens, James C, Dixon, Jack E
Despite the wealth of information generated by genome-sequencing projects, the identification of in vivo substrates of specific protein kinases and phosphatases is hampered by the large number of...
Begley, Michael J., Taylor, Gregory S., Brock, Melissa A., Ghosh, Partho, Woods, Virgil L., Dixon, Jack E.
Myotubularins, a large family of catalytically active and inactive proteins, belong to a unique subgroup of protein tyrosine phosphatases that use inositol phospholipids, rather than phosphoproteins,...
Paulsen, Michelle T, Starks, Adrienne M, Derheimer, Frederick A, Hanasoge, Sheela, Li, Liwu, Dixon, Jack E, ...
The Sorcerer II Global Ocean Sampling Expedition: Expanding the Universe of Protein Families
Yooseph, Shibu, Sutton, Granger, Rusch, Douglas B, Halpern, Aaron L, Williamson, Shannon J, Remington, Karin, ...
Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a...
Wiley, Sandra E., Murphy, Anne N., Ross, Stuart A., Van Der Geer, Peter, Dixon, Jack E.
Members of the thiazolidinedione (TZD) class of insulin-sensitizing drugs are extensively used in the treatment of type 2 diabetes. Pioglitazone, a member of the TZD family, has been shown to bind...
A conserved phosphatase cascade that regulates nuclear membrane biogenesis
Kim, Youngjun, Gentry, Matthew S., Harris, Thurl E., Wiley, Sandra E., Lawrence, John C., Dixon, Jack E.
A newly emerging family of phosphatases that are members of the haloacid dehalogenase superfamily contains the catalytic motif DXDX(T/V). A member of this DXDX(T/V) phosphatase family known as...
Gentry, Matthew S., Dowen, Robert H., Worby, Carolyn A., Mattoo, Seema, Ecker, Joseph R., Dixon, Jack E.
Lafora disease (LD) is a progressive myoclonic epilepsy resulting in severe neurodegeneration followed by death. A hallmark of LD is the accumulation of insoluble polyglucosans called Lafora bodies...
MitoNEET is a uniquely folded 2Fe–2S outer mitochondrial membrane protein stabilized by pioglitazone
Paddock, Mark L., Wiley, Sandra E., Axelrod, Herbert L., Cohen, Aina E., Roy, Melinda, Abresch, Edward C., ...
Iron–sulfur (Fe–S) proteins are key players in vital processes involving energy homeostasis and metabolism from the simplest to most complex organisms. We report a 1.5 Å x-ray crystal structure...
Alto, Neal M., Weflen, Andrew W., Rardin, Matthew J., Yarar, Defne, Lazar, Cheri S., Tonikian, Raffi, ...
Bacterial toxins and effector proteins hijack eukaryotic enzymes that are spatially localized and display rapid signaling kinetics. However, the molecular mechanisms by which virulence factors engage...
A role for AGL ubiquitination in the glycogen storage disorders of Lafora and Cori’s disease
Cheng, Alan, Zhang, Mei, Gentry, Matthew S., Worby, Carolyn A., Dixon, Jack E., Saltiel, Alan R.
Cori’s disease is a glycogen storage disorder characterized by a deficiency in the glycogen debranching enzyme, amylo-1,6-glucosidase,4-α-glucanotransferase (AGL). Here, we demonstrate that the...
Robinson, Fred L., Niesman, Ingrid R., Beiswenger, Kristina K., Dixon, Jack E.
Charcot–Marie–Tooth disease type 4B (CMT4B) is a severe, demyelinating peripheral neuropathy characterized by slowed nerve conduction velocity, axon loss, and distinctive myelin outfolding and...
Rardin, Matthew J., Wiley, Sandra E., Murphy, Anne N., Pagliarini, David J., Dixon, Jack E.
Although large-scale approaches have identified numerous mitochondrial phosphoproteins, little is known about the mitochondrial kinases and phosphatases that regulate these phosphoproteins. Here, we...
Discovery of a widely distributed toxin biosynthetic gene cluster
Lee, Shaun W., Mitchell, Douglas A., Markley, Andrew L., Hensler, Mary E., Gonzalez, David, Wohlrab, Aaron, ...
Bacteriocins represent a large family of ribosomally produced peptide antibiotics. Here we describe the discovery of a widely conserved biosynthetic gene cluster for the synthesis of thiazole and...
Iwasaki, Hirohide, Murata, Yoshimichi, Kim, Youngjun, Hossain, Md. Israil, Worby, Carolyn A., Dixon, Jack E., ...
Phosphatidylinositol lipids play diverse physiological roles, and their concentrations are tightly regulated by various kinases and phosphatases. The enzymatic activity of Ciona intestinalis voltage...
Structural and Functional Dissection of the Heterocyclic Peptide Cytotoxin Streptolysin S*S⃞
Mitchell, Douglas A., Lee, Shaun W., Pence, Morgan A., Markley, Andrew L., Limm, Joyce D., Nizet, Victor, ...
The human pathogen Streptococcus pyogenes secretes a highly cytolytic toxin known as streptolysin S (SLS). SLS is a key virulence determinant and responsible for the β-hemolytic phenotype of these...
Dowen, Robert H., Engel, James L., Shao, Feng, Ecker, Joseph R., Dixon, Jack E.
Bacterial phytopathogens employ a type III secretion system to deliver effector proteins into the plant cell to suppress defense pathways; however, the molecular mechanisms and subcellular...