Kasai, Masataka, Aokl, Katsunori, Matsuo, Yoshinobu, Minowada, Jun, Maziarz, Richard T., Strominger, Jack L.
A DNA binding protein was identified which binds to two novel target-like sequences: (I) at the 5′ flanking site of the breakpoint junction of chromosome 8 in a patient with T-acute lymphoblastlc...
Chicz, Roman M., Lane, William S., Robinson, Renée A., Trucco, Massimo, Strominger, Jack L., Gorga, Joan C.
Genetic susceptibility to several autoimmune disorders is associated with the expression of certain MHC class II alleles. Insight into the etiology of such diseases awaits the identification of the...
Fabbi, Marina, Groh, Veronika, Strominger, Jack L.
The proliferation potential of highly purified human CD3−CD4−CD8− (triple negative) and CD3low(lo)CD4−CD8− thymocyte precursors in response to various cytokines was investigated. High in...
Bray, Robert A., Kornbluth, Jacki, Landay, Alan L., Strominger, Jack L., Gebel, Howard, Seidman, Jon G., ...
To test the hypothesis that the T-cell receptor ( Tcr ) λ gene encodes a natural killer (NK) cell receptor molecule, three human NK clones and fresh peripheral blood lymphocytes with NK activity...
Structure of Class I and Class II HLA Antigens (1987)
Considerable information is now available about the overall structure of both class I and class II HLA antigens and in the not too distant future should be available in atomic detail in the crystal...
Wilson, S. Brian, Kent, Sally C., Horton, Heidi F., Hill, Andrew A., Bollyky, Paul L., Hafler, David A., ...
Quantitative and qualitative defects in CD1d-restricted T cells have been demonstrated in human and murine autoimmune diseases. To investigate the transcriptional consequences of T cell receptor...
Mandelboim, Ofer, Kent, Sally, Davis, Daniel M., Wilson, S. Brian, Okazaki, Taku, Jackson, Richard, ...
Proliferation of human CD4+ αβ T cells expressing a natural killer cell activating receptor (NKAR) has been shown to be enhanced, particularly in response to low doses of antigen, if the target...
Self and viral peptides can initiate lysis by autologous natural killer cells
Mandelboim, Ofer, Wilson, S. Brian, Valés-Gómez, Mar, Reyburn, Hugh T., Strominger, Jack L.
Natural killer (NK) cells are inhibited by specific allotypes of class I major histocompatibility complex ligands recognized by polymorphic inhibitory receptors (e.g., NKIR1 and NKIR2). NK1- and...
Mahanta, Sanjeev K., Scholl, Thomas, Yang, Fu-Chia, Strominger, Jack L.
CIITA is a positive regulator of class II major histocompatibility complex gene transcription that has been found to be defective in one of the five complementation groups of class II major...
Scholl, Thomas, Mahanta, Sanjeev K., Strominger, Jack L.
Two of the genes defective in the five complementation groups identified in the class II-negative bare lymphocyte syndrome or corresponding laboratory mutants have been cloned. One gene encodes a...
Gauthier, Laurent, Smith, Kathrine J., Pyrdol, Jason, Kalandadze, Avtandil, Strominger, Jack L., Wiley, Don C., ...
HLA-DR2 is associated with susceptibility to multiple sclerosis (MS). A peptide from human myelin basic protein (MBP, residues 85–99) was previously found to bind to purified HLA-DR2 (DRA,...
Human CD16 as a lysis receptor mediating direct natural killer cell cytotoxicity
Mandelboim, Ofer, Malik, Pratap, Davis, Daniel M., Jo, Chang H., Boyson, Jonathan E., Strominger, Jack L.
In addition to their role in peptide antigen presentation, class I MHC proteins also play a critical role in inhibiting natural killer (NK) cytotoxicity through interaction with NK inhibitory...
Heterophile antibodies segregate in families and are associated with protection from type 1 diabetes
She, Jin-Xiong, Ellis, Tamir M., Wilson, S. Brian, Wasserfall, Clive H., Marron, Michele, Reimsneider, Sharon, ...
Markedly elevated levels of serum IL-4 were reported previously in 50% of a small group of type 1 diabetes nonprogessors. To determine the patterns of expression for this phenotype, a larger cohort...
Fridkis-Hareli, Masha, Rosloniec, Edward F., Fugger, Lars, Strominger, Jack L.
Copolymer 1 [poly(Y,E,A,K)] is a random synthetic amino acid copolymer of l-tyrosine, l-glutamic acid, l-alanine, and l-lysine that is effective both in suppression of experimental allergic...
Trafficking of spontaneously endocytosed MHC proteins
Chiu, Isaac, Davis, Daniel M., Strominger, Jack L.
Class I MHC protein primarily presents endogenous antigen but also may present exogenous antigen. Here, we investigated the intracellular pathway of spontaneously internalized class I MHC protein by...
Porgador, Angel, Mandelboim, Ofer, Restifo, Nicholas P., Strominger, Jack L.
Cancer vaccines used to generate specific cytotoxic T lymphocytes are not effective against tumor cells that have lost or suppressed expression of their class I major histocompatibility complex...
Extracellular antigen processing and presentation by immature dendritic cells
Santambrogio, Laura, Sato, Aaron K., Carven, Gregory J., Belyanskaya, Svetlana L., Strominger, Jack L., Stern, Lawrence J.
In antigen presentation to CD4+ T cells, proteins are degraded to peptide fragments and loaded onto class II MHC molecules in a process involving the peptide exchange factors H-2M (murine) or HLA-DM...
The human natural killer cell immune synapse
Davis, Daniel M., Chiu, Isaac, Fassett, Marlys, Cohen, George B., Mandelboim, Ofer, Strominger, Jack L.
Inhibitory killer Ig-like receptors (KIR) at the surface of natural killer (NK) cells induced clustering of HLA-C at the contacting surface of target cells. In this manner, inhibitory immune synapses...
Superactivation of an immune response triggered by oligomerized T cell epitopes
Rötzschke, Olaf, Falk, Kirsten, Strominger, Jack L.
The peptides bound to class II major histocompatibility complex (MHC) molecules extend out both ends of the peptide binding groove. This structural feature provided the opportunity to design...
The role of zinc in the binding of killer cell Ig-like receptors to class I MHC proteins
Valés-Gómez, Mar, Erskine, Robert A., Deacon, Matthew P., Strominger, Jack L., Reyburn, Hugh T.
The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During...
Germ line deletion of the CD1 locus exacerbates diabetes in the NOD mouse
Shi, Fu-Dong, Flodström, Malin, Balasa, Balaji, Kim, Soon Ha, Van Gunst, Kurt, Strominger, Jack L., ...
Quantitative and qualitative defects in CD1-restricted natural killer T cells have been reported in several autoimmune-prone strains of mice, including the nonobese diabetic (NOD) mouse. These...
Naumov, Yuri N., Bahjat, Keith S., Gausling, Rudolph, Abraham, Roshini, Exley, Mark A., Koezuka, Yasuhiko, ...
CD1d-restricted invariant NKT (iNKT) cells are immunoregulatory cells whose loss exacerbates diabetes in nonobese diabetic (NOD) female mice. Here, we show that the relative numbers of iNKT cells...
Fassett, Marlys S., Davis, Daniel M., Valter, Markus M., Cohen, George B., Strominger, Jack L.
Natural killer (NK) cell cytotoxicity is determined by a balance of positive and negative signals. Negative signals are transmitted by NK inhibitory receptors (killer immunoglobulin-like receptors,...
Orange, Jordan S., Ramesh, Narayanaswamy, Remold-O'Donnell, Eileen, Sasahara, Yoji, Koopman, Louise, Byrne, Michael, ...
The Wiskott–Aldrich syndrome (WAS) is a primary immunodeficiency disorder caused by a mutation in WAS protein (WASp) that results in defective actin polymerization. Although the function of many...
CD1d and invariant NKT cells at the human maternal–fetal interface
Boyson, Jonathan E., Rybalov, Basya, Koopman, Louise A., Exley, Mark, Balk, Steven P., Racke, Frederick K., ...
Invariant CD1d-restricted natural killer T (iNKT) cells comprise a small, but significant, immunoregulatory T cell subset. Here, the presence of these cells and their CD1d ligand at the human...
Disulfide bond-mediated dimerization of HLA-G on the cell surface
Boyson, Jonathan E., Erskine, Robert, Whitman, Mary C., Chiu, Michael, Lau, Julie M., Koopman, Louise A., ...
HLA-G is a nonclassical class I MHC molecule with an unknown function and with unusual characteristics that distinguish it from other class I MHC molecules. Here, we demonstrate that HLA-G forms...
A pH-sensitive histidine residue as control element for ligand release from HLA-DR molecules
Rötzschke, Olaf, Lau, Julie M., Hofstätter, Maria, Falk, Kirsten, Strominger, Jack L.
Class II MHC molecules undergo conformational changes on shifts of the pH. As a consequence, low-affinity peptides tightly bound at pH 7.0 can be released at pH 5.0. The imidazole group of histidine...
Deficient natural killer cell cytotoxicity in patients with IKK-γ/NEMO mutations
Orange, Jordan S., Brodeur, Scott R., Jain, Ashish, Bonilla, Francisco A., Schneider, Lynda C., Kretschmer, Roberto, ...
NF-κB essential modifier (NEMO), also known as IKK-γ, is a member of the I-κB kinase complex responsible for phosphorylating I-κB, allowing the release and activation of NF-κB. Boys with an...
Fridkis-Hareli, Masha, Santambrogio, Laura, Stern, Joel N.H., Fugger, Lars, Brosnan, Celia, Strominger, Jack L.
Copolymer 1 (Cop 1, Copaxone [Teva Marion Partners, Kansas City, Missouri, USA]), a random amino acid copolymer of tyrosine (Y), glutamic acid (E), alanine (A), and lysine (K), reduces the frequency...
Gillessen, Silke, Naumov, Yuri N., Nieuwenhuis, Edward E. S., Exley, Mark A., Lee, Frederick S., Mach, Nicolas, ...
CD1d-restricted T cells contribute to tumor protection, but their precise roles remain unclear. Here we show that tumor cells engineered to secrete granulocyte–macrophage colony-stimulating factor...
Spratt, Brian G., Strominger, Jack L.
Penicillin-binding proteins 5 and 6 of Escherichia coli have been identified as d-alanine carboxypeptidase IA.
Buchanan, Christine E., Hsia, Judy, Strominger, Jack L.
The fact that antibody to d-alanine carboxypeptidase of Bacillus subtilis does not cross-react with other penicillin-binding proteins suggests that these proteins are not precursors or multimers of...
Wickus, Gary G., Warth, Alan D., Strominger, Jack L.
Evidence is presented for the appearance of muramic lactam during the late stages of sporulation at about the same time dipicolinic acid synthesis occurs.
Isolation of Polyisoprenyl Alcohols from Streptococcus faecalis
Umbreit, Jay N., Stone, K. John, Strominger, Jack L.
C55-isoprenyl alcohol and its derivatives have been isolated from Streptococcus faecalis and characterized. The relative amounts present as free alcohol, neutral lipid esters, and phosphate ester...
Isolation of the Lipid Intermediate in Peptidoglycan Biosynthesis from Escherichia coli
Umbreit, Jay N., Strominger, Jack L.
The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C55-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine.
Wickus, Gary G., Strominger, Jack L.
A method for the partial purification of the pyruvate-uridine-diphospho-N-acetylglucosamine transferase from Staphylococcus epidermidis is presented. Some properties of the enzyme are discussed.
Wickus, Gary G., Rubenstein, Peter A., Warth, Alan D., Strominger, Jack L.
A method for the partial purification of the uridine diphospho-N-acetylglucosamine-enolpyruvate reductase from Staphylococcus epidermidis is presented. Some properties of the enzyme, including its...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes were prepared from cells grown as spheres and from peptone- and succinate-induced rod stage cells. Undegraded polysaccharide backbones of the...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes grown as spheres and as rods were solubilized by treatment with the B enzyme from Chalaropsis, an N-acetylmuramidase. The neutral glycopeptides were then...
Biochemical Effects of Novobiocin on Staphylococcus aureus
Wishnow, Rodney M., Strominger, Jack L., Birge, Claire H., Threnn, Robert H.
Wishnow, Rodney M. (Washington University School of Medicine, St. Louis, Mo.), Jack L. Strominger, Claire H. Birge, and Robert H. Threnn. Biochemical effects of novobiocin on Staphylococcus aureus....
Nucleotide Accumulation Induced in Staphylococcus aureus by Glycine
Strominger, Jack L., Birge, Claire H.
Strominger, Jack L. (Washington University School of Medicine, St. Louis, Mo.), and Claire H. Birge. Nucleotide accumulation induced in Staphylococcus aureus by glycine. J. Bacteriol. 89:1124–1127....
Okazaki, Tuneko, Strominger, Jack L., Okazaki, Reiji
Okazaki, Tuneko (Washington University School of Medicine, St. Louis, Mo.), Jack L. Stominger, and Reiji Okazaki. Thymidine diphosphate-l-rhamnose metabolism in smooth and rough strains of...
EPIMERIZATION OF THYMIDINE DIPHOSPHATE GLUCOSE IN BACTERIAL EXTRACTS
Tinelli, Regina, Michelson, A. M., Strominger, Jack L.
Tinelli, Regina (Washington University School of Medicine, St. Louis, Mo.), A. M. Michelson, and Jack L. Strominger. Epimerization of thymidine diphosphate glucose in bacterial extracts. J....
The mature activating natural killer cell immunologic synapse is formed in distinct stages
Orange, Jordan S., Harris, K. Eliza, Andzelm, Milena M., Valter, Markus M., Geha, Raif S., Strominger, Jack L.
Natural killer (NK) cells form a structure at their interface with a susceptible target cell called the activating NK cell immunologic synapse (NKIS). The mature activating NKIS contains a central...
Kamiryo, Tatsuyuki, Strominger, Jack L.
A group of Escherichia coli mutants which are ampicillin resistant at 32 C and which either are unable to grow or lyse at 42 C has been selected. These mutants have been classified by a number of...
Mechanism of Action and Development of Resistance to a New Amidino Penicillin
Matsuhashi, Sachiko, Kamiryo, Tatsuyuki, Blumberg, Peter M., Linnett, Paul, Willoughby, Eileen, Strominger, Jack L.
The mechanism of killing of Escherichia coli by a novel beta-lactam antibiotic, an amidino penicillin, has been investigated. This compound converts E. coli to relatively stable spherical forms at...
Storm, Daniel R., Blumberg, Peter M., Strominger, Jack L.
An insoluble penicillin derivative, 6-aminopenicillanic acid covalently coupled to Sepharose, was used to investigate the location of the d-alanine carboxypeptidase in the Bacillus subtilis membrane....
Penicillin Binding Components in Bacillius subtilis During Sporulation
Anwar, Rashid A., Blumberg, Peter M., Strominger, Jack L.
The penicillin binding components of sporulating Bacillus subtilis 3610 were compared with those of the vegetative organism. No qualitative difference in binding components was observed. The total...
d-Alanine Carboxypeptidase and Cell Wall Cross-Linking in Bacillus subtilis
Sharpe, Arlene, Blumberg, Peter M., Strominger, Jack L.
Inhibition of d-alanine carboxypeptidase in Bacillus subtilis by 95% caused no measurable change in the degree of cross-linking of the peptidoglycan.
Specificity of a Bacteriolytic Enzyme from Pseudomonas aeruginosa1
Lache, Marvin, Hearn, Walter R., Zyskind, Judith W., Tipper, Donald J., Strominger, Jack L.
A bacteriolytic enzyme isolated from shake-flask cultures of Pseudomonas aeruginosa and capable of lysing cells of Staphylococcus aureus was purified approximately 500-fold by passage through...
Shackelford, Deborah A., Mann, Dean L., Van Rood, Jon J., Ferrara, G. B., Strominger, Jack L.
Some human B-lymphoblastoid cell lines are shown to express at least two types of Ia-like antigens. One antigen is defined by alloantisera to HLA-DR, and the other antigen is defined by alloantisera...
MLL 5 protein forms intranuclear foci, and overexpression inhibits cell cycle progression
Deng, Lih-Wen, Chiu, Isaac, Strominger, Jack L.
MLL5 is a mammalian trithorax group (trx-G) gene identified within chromosome band 7q22, a frequently deleted element found in cytogenetic aberrations of acute myeloid malignancies. MLL5 cDNA was...
Rosenthal, Ken S., Yanovich, Saul, Inbar, Michael, Strominger, Jack L.
Translocation of the hydrocarbon fluorescent probe diphenylhexatriene (DPH) between membranes was studied by fluorescence polarization (P) analysis. First, using a model system, the high P value...
Siebold, Christian, Hansen, Bjarke E., Wyer, Jessica R., Harlos, Karl, Esnouf, Robert E., Svejgaard, Arne, ...
The MHC class II molecule DQ0602 confers strong susceptibility to narcolepsy but dominant protection against type 1 diabetes. The crystal structure of DQ0602 reveals the molecular features underlying...
Partial Purification of Detergent-Soluble HL-A Antigen and Its Cleavage by Papain
Springer, Timothy A., Strominger, Jack L., Mann, Dean
HL-A antigen solubilized with the non-ionic detergent, Brij 99, has been purified to about 50% of homogeneity from a cultured human lymphoblast line. It consists of two nonidentical subunits of...
Cresswell, Peter, Springer, Timothy, Strominger, Jack L., Turner, Mervyn J., Grey, Howard M., Kubo, Ralph T.
A number of immunological methods have been employed to show that the small subunit of HL-A antigens, isolated either after papain treatment or after solubilization with detergent, is identical to...
C55-Isoprenoid Alcohol Phosphokinase: An Extremely Hydrophobic Protein from the Bacterial Membrane
Sandermann, Heinrich, Strominger, Jack L.
C55-isoprenoid alcohol phosphokinase, a butanol-soluble enzyme from the membrane of Staphylococcus aureus, has been purified to homogeneity by the inclusion of organic solvents in all of the...
Blumberg, Peter M., Strominger, Jack L.
The D-alanine carboxypeptidase of Bacillus subtilis is a particulate enzyme that is irreversibly inactivated by penicillins and cephalosporins. However, the lethal concentrations of these antibiotics...
Mechanism of Action of Bacitracin: Complexation with Metal Ion and C55-Isoprenyl Pyrophosphate
Stone, K. John, Strominger, Jack L.
The inhibition by bacitracin of the enzymatic dephosphorylation of C55-isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a...
Blumberg, Peter M., Strominger, Jack L.
An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet...
Rasmussen, James R., Strominger, Jack L.
The penicillin-sensitive D-alanine carboxypeptidases of Bacillus subtilis, Escherichia coli, and Staphylococcus aureus catalyzed the hydrolysis of the D-lactic acid residue from the depsipeptide...
Inhibition of Sterol Biosynthesis by Bacitracin
Stone, K. John, Strominger, Jack L.
Bacitracin is an inhibitor of the biosynthesis of squalene and sterols from mevalonic acid, C5-isopentenyl pyrophosphate, or C15-farnesyl pyrophosphate catalyzed by preparations from rat liver. The...
Gonzalez-Porque, Pedro, Strominger, Jack L.
A cofactor needed for the reduction of CDP-4-keto-6-deoxy-D-glucose to CDP-4-keto-3,6-dideoxy D-glucose has been detected and purified. Its spectra in neutral, acid, and basic solutions, its behavior...
Umbreit, Jay N., Strominger, Jack L.
Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay....
Umbreit, Jay N., Strominger, Jack L.
The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a...
Papain-Solubilized HL-A Antigens from Cultured Human Lymphocytes Contain Two Peptide Fragments
Cresswell, Peter, Turner, Mervyn J., Strominger, Jack L.
HL-A 2 and HL-A 7 antigens have been solubilized from cultured human lymphocytes by papain and purified on a small radioactive scale after formation of immune complexes, or on a large scale by column...
Wedgwood, Josiah F., Warren, Christopher D., Jeanloz, Roger W., Strominger, Jack L.
Fully acetylated chitobiose was treated with phosphoric acid to give a mixture of products from which 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-...
Heterogeneity of HL-A Antigen Preparations Is Due to Variable Sialic Acid Content
Parham, Peter, Humphreys, Robert E., Turner, Mervyn J., Strominger, Jack L.
Purified, papain-solubilized preparations of HL-A antigen from cultured human lymphoblastoid cells (HL-A2 and HL-A7,12 from RPMI 4265 cells and a mixture of HL-A3, W-25, HL-A12, and HL-A27 from IM-1...
Additional Antibiotic Inhibitors of Peptidoglycan Synthesis
Linnett, Paul E., Strominger, Jack L.
Diumycin, janiemycin, nisin, and subtilin inhibited peptidoglycan synthesis catalyzed by particulate enzyme systems from Bacillus stearothermophilus and Escherichia coli. All of these, except for...
Stern, Joel N. H., Illés, Zsolt, Reddy, Jayagopala, Keskin, Derin B., Sheu, Eric, Fridkis-Hareli, Masha, ...
Copolymer 1 [Cop1, glatiramer acetate, Copaxone, poly(Y,E,A,K)n] is widely used in the treatment of relapsing/remitting multiple sclerosis in which it reduces the frequency of relapses by ≈30%. In...
Illés, Zsolt, Stern, Joel N. H., Reddy, Jayagopala, Waldner, Hanspeter, Mycko, Marcin P., Brosnan, Celia F., ...
A humanized mouse bearing the HLA-DR2 (DRA/DRB1*1501) protein associated with multiple sclerosis (MS) and the myelin basic protein (MBP) 85–99-specific HLA-DR2-restricted T cell receptor from an MS...
Wong, Siew Heng, Santambrogio, Laura, Strominger, Jack L.
The passage of dendritic cells (DC) from immature to terminally differentiated antigen-presenting cells is accompanied by numerous morphological, phenotypic, and functional changes. These changes...
Stern, Joel N. H., Illés, Zsolt, Reddy, Jayagopala, Keskin, Derin B., Fridkis-Hareli, Masha, Kuchroo, Vijay K., ...
Myelin basic protein (MBP) is a major candidate autoantigen in multiple sclerosis (MS). Its immunodominant epitope, MBP 85–99, forms a complex with human leukocyte antigen (HLA)-DR2 with which...
Smith, Cassandra E., Eagar, Todd N., Strominger, Jack L., Miller, Stephen D.
The ability of different forms of myelin peptides to induce tolerance for the treatment of preestablished murine experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis,...
Vieillard, Vincent, Strominger, Jack L., Debré, Patrice
HIV infection leads to a state of chronic immune activation and progressive deterioration in immune function, manifested most recognizably by the progressive depletion of CD4+ T cells. A substantial...
Human decidual NK cells form immature activating synapses and are not cytotoxic
Kopcow, Hernan D., Allan, David S. J., Chen, Xi, Rybalov, Basya, Andzelm, Milena M., Ge, Baoxue, ...
In early pregnancy invading fetal trophoblasts encounter abundant maternal decidual natural killer cells (dNK). dNK express perforin, granzymes A and B and the activating receptors NKp30, NKp44,...
Nucleotide Intermediates in the Biosynthesis of Heteropolymeric Polysaccharides
The role of nucleotides as “carriers” of small molecules for biosynthetic reactions is discussed. Following this introduction, the particular problem of nucleotide intermediates in chondroitin...
Nishimura, Hiroyuki, Strominger, Jack L.
Human systemic lupus erythematosus (SLE) and its murine model, MRL lpr/lpr mice, are well known to develop a wide range of symptoms, such as glomerulonephritis, dermatitis, and arthritis, as an...
Gestation stage-dependent mechanisms of invariant natural killer T cell-mediated pregnancy loss
Boyson, Jonathan E., Nagarkatti, Nisha, Nizam, Leena, Exley, Mark A., Strominger, Jack L.
Stimulation of CD1d-restricted semiinvariant natural killer T cells by using the CD1d ligand α-galactosylceramide (αGalCer) induces pregnancy loss in mice through an ill-defined mechanism involving...
Wilson, S. Brian, Kent, Sally C., Horton, Heidi F., Hill, Andrew A., Bollyky, Paul L., Hafler, David A., ...
Quantitative and qualitative defects in CD1d-restricted T cells have been demonstrated in human and murine autoimmune diseases. To investigate the transcriptional consequences of T cell receptor...
Mandelboim, Ofer, Kent, Sally, Davis, Daniel M., Wilson, S. Brian, Okazaki, Taku, Jackson, Richard, ...
Proliferation of human CD4+ αβ T cells expressing a natural killer cell activating receptor (NKAR) has been shown to be enhanced, particularly in response to low doses of antigen, if the target...
Self and viral peptides can initiate lysis by autologous natural killer cells
Mandelboim, Ofer, Wilson, S. Brian, Valés-Gómez, Mar, Reyburn, Hugh T., Strominger, Jack L.
Natural killer (NK) cells are inhibited by specific allotypes of class I major histocompatibility complex ligands recognized by polymorphic inhibitory receptors (e.g., NKIR1 and NKIR2). NK1- and...
Mahanta, Sanjeev K., Scholl, Thomas, Yang, Fu-Chia, Strominger, Jack L.
CIITA is a positive regulator of class II major histocompatibility complex gene transcription that has been found to be defective in one of the five complementation groups of class II major...
Scholl, Thomas, Mahanta, Sanjeev K., Strominger, Jack L.
Two of the genes defective in the five complementation groups identified in the class II-negative bare lymphocyte syndrome or corresponding laboratory mutants have been cloned. One gene encodes a...
Gauthier, Laurent, Smith, Kathrine J., Pyrdol, Jason, Kalandadze, Avtandil, Strominger, Jack L., Wiley, Don C., ...
HLA-DR2 is associated with susceptibility to multiple sclerosis (MS). A peptide from human myelin basic protein (MBP, residues 85–99) was previously found to bind to purified HLA-DR2 (DRA,...
Human CD16 as a lysis receptor mediating direct natural killer cell cytotoxicity
Mandelboim, Ofer, Malik, Pratap, Davis, Daniel M., Jo, Chang H., Boyson, Jonathan E., Strominger, Jack L.
In addition to their role in peptide antigen presentation, class I MHC proteins also play a critical role in inhibiting natural killer (NK) cytotoxicity through interaction with NK inhibitory...
Heterophile antibodies segregate in families and are associated with protection from type 1 diabetes
She, Jin-Xiong, Ellis, Tamir M., Wilson, S. Brian, Wasserfall, Clive H., Marron, Michele, Reimsneider, Sharon, ...
Markedly elevated levels of serum IL-4 were reported previously in 50% of a small group of type 1 diabetes nonprogessors. To determine the patterns of expression for this phenotype, a larger cohort...
Fridkis-Hareli, Masha, Rosloniec, Edward F., Fugger, Lars, Strominger, Jack L.
Copolymer 1 [poly(Y,E,A,K)] is a random synthetic amino acid copolymer of l-tyrosine, l-glutamic acid, l-alanine, and l-lysine that is effective both in suppression of experimental allergic...
Trafficking of spontaneously endocytosed MHC proteins
Chiu, Isaac, Davis, Daniel M., Strominger, Jack L.
Class I MHC protein primarily presents endogenous antigen but also may present exogenous antigen. Here, we investigated the intracellular pathway of spontaneously internalized class I MHC protein by...
Porgador, Angel, Mandelboim, Ofer, Restifo, Nicholas P., Strominger, Jack L.
Cancer vaccines used to generate specific cytotoxic T lymphocytes are not effective against tumor cells that have lost or suppressed expression of their class I major histocompatibility complex...
Extracellular antigen processing and presentation by immature dendritic cells
Santambrogio, Laura, Sato, Aaron K., Carven, Gregory J., Belyanskaya, Svetlana L., Strominger, Jack L., Stern, Lawrence J.
In antigen presentation to CD4+ T cells, proteins are degraded to peptide fragments and loaded onto class II MHC molecules in a process involving the peptide exchange factors H-2M (murine) or HLA-DM...
The human natural killer cell immune synapse
Davis, Daniel M., Chiu, Isaac, Fassett, Marlys, Cohen, George B., Mandelboim, Ofer, Strominger, Jack L.
Inhibitory killer Ig-like receptors (KIR) at the surface of natural killer (NK) cells induced clustering of HLA-C at the contacting surface of target cells. In this manner, inhibitory immune synapses...
Superactivation of an immune response triggered by oligomerized T cell epitopes
Rötzschke, Olaf, Falk, Kirsten, Strominger, Jack L.
The peptides bound to class II major histocompatibility complex (MHC) molecules extend out both ends of the peptide binding groove. This structural feature provided the opportunity to design...
The role of zinc in the binding of killer cell Ig-like receptors to class I MHC proteins
Valés-Gómez, Mar, Erskine, Robert A., Deacon, Matthew P., Strominger, Jack L., Reyburn, Hugh T.
The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During...
Germ line deletion of the CD1 locus exacerbates diabetes in the NOD mouse
Shi, Fu-Dong, Flodström, Malin, Balasa, Balaji, Kim, Soon Ha, Van Gunst, Kurt, Strominger, Jack L., ...
Quantitative and qualitative defects in CD1-restricted natural killer T cells have been reported in several autoimmune-prone strains of mice, including the nonobese diabetic (NOD) mouse. These...
Naumov, Yuri N., Bahjat, Keith S., Gausling, Rudolph, Abraham, Roshini, Exley, Mark A., Koezuka, Yasuhiko, ...
CD1d-restricted invariant NKT (iNKT) cells are immunoregulatory cells whose loss exacerbates diabetes in nonobese diabetic (NOD) female mice. Here, we show that the relative numbers of iNKT cells...
Fassett, Marlys S., Davis, Daniel M., Valter, Markus M., Cohen, George B., Strominger, Jack L.
Natural killer (NK) cell cytotoxicity is determined by a balance of positive and negative signals. Negative signals are transmitted by NK inhibitory receptors (killer immunoglobulin-like receptors,...
Orange, Jordan S., Ramesh, Narayanaswamy, Remold-O'Donnell, Eileen, Sasahara, Yoji, Koopman, Louise, Byrne, Michael, ...
The Wiskott–Aldrich syndrome (WAS) is a primary immunodeficiency disorder caused by a mutation in WAS protein (WASp) that results in defective actin polymerization. Although the function of many...
CD1d and invariant NKT cells at the human maternal–fetal interface
Boyson, Jonathan E., Rybalov, Basya, Koopman, Louise A., Exley, Mark, Balk, Steven P., Racke, Frederick K., ...
Invariant CD1d-restricted natural killer T (iNKT) cells comprise a small, but significant, immunoregulatory T cell subset. Here, the presence of these cells and their CD1d ligand at the human...
Disulfide bond-mediated dimerization of HLA-G on the cell surface
Boyson, Jonathan E., Erskine, Robert, Whitman, Mary C., Chiu, Michael, Lau, Julie M., Koopman, Louise A., ...
HLA-G is a nonclassical class I MHC molecule with an unknown function and with unusual characteristics that distinguish it from other class I MHC molecules. Here, we demonstrate that HLA-G forms...
A pH-sensitive histidine residue as control element for ligand release from HLA-DR molecules
Rötzschke, Olaf, Lau, Julie M., Hofstätter, Maria, Falk, Kirsten, Strominger, Jack L.
Class II MHC molecules undergo conformational changes on shifts of the pH. As a consequence, low-affinity peptides tightly bound at pH 7.0 can be released at pH 5.0. The imidazole group of histidine...
Deficient natural killer cell cytotoxicity in patients with IKK-γ/NEMO mutations
Orange, Jordan S., Brodeur, Scott R., Jain, Ashish, Bonilla, Francisco A., Schneider, Lynda C., Kretschmer, Roberto, ...
NF-κB essential modifier (NEMO), also known as IKK-γ, is a member of the I-κB kinase complex responsible for phosphorylating I-κB, allowing the release and activation of NF-κB. Boys with an...
Fridkis-Hareli, Masha, Santambrogio, Laura, Stern, Joel N.H., Fugger, Lars, Brosnan, Celia, Strominger, Jack L.
Copolymer 1 (Cop 1, Copaxone [Teva Marion Partners, Kansas City, Missouri, USA]), a random amino acid copolymer of tyrosine (Y), glutamic acid (E), alanine (A), and lysine (K), reduces the frequency...
Gillessen, Silke, Naumov, Yuri N., Nieuwenhuis, Edward E. S., Exley, Mark A., Lee, Frederick S., Mach, Nicolas, ...
CD1d-restricted T cells contribute to tumor protection, but their precise roles remain unclear. Here we show that tumor cells engineered to secrete granulocyte–macrophage colony-stimulating factor...
Spratt, Brian G., Strominger, Jack L.
Penicillin-binding proteins 5 and 6 of Escherichia coli have been identified as d-alanine carboxypeptidase IA.
Buchanan, Christine E., Hsia, Judy, Strominger, Jack L.
The fact that antibody to d-alanine carboxypeptidase of Bacillus subtilis does not cross-react with other penicillin-binding proteins suggests that these proteins are not precursors or multimers of...
Wickus, Gary G., Warth, Alan D., Strominger, Jack L.
Evidence is presented for the appearance of muramic lactam during the late stages of sporulation at about the same time dipicolinic acid synthesis occurs.
Isolation of Polyisoprenyl Alcohols from Streptococcus faecalis
Umbreit, Jay N., Stone, K. John, Strominger, Jack L.
C55-isoprenyl alcohol and its derivatives have been isolated from Streptococcus faecalis and characterized. The relative amounts present as free alcohol, neutral lipid esters, and phosphate ester...
Isolation of the Lipid Intermediate in Peptidoglycan Biosynthesis from Escherichia coli
Umbreit, Jay N., Strominger, Jack L.
The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C55-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine.
Wickus, Gary G., Strominger, Jack L.
A method for the partial purification of the pyruvate-uridine-diphospho-N-acetylglucosamine transferase from Staphylococcus epidermidis is presented. Some properties of the enzyme are discussed.
Wickus, Gary G., Rubenstein, Peter A., Warth, Alan D., Strominger, Jack L.
A method for the partial purification of the uridine diphospho-N-acetylglucosamine-enolpyruvate reductase from Staphylococcus epidermidis is presented. Some properties of the enzyme, including its...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes were prepared from cells grown as spheres and from peptone- and succinate-induced rod stage cells. Undegraded polysaccharide backbones of the...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes grown as spheres and as rods were solubilized by treatment with the B enzyme from Chalaropsis, an N-acetylmuramidase. The neutral glycopeptides were then...
Biochemical Effects of Novobiocin on Staphylococcus aureus
Wishnow, Rodney M., Strominger, Jack L., Birge, Claire H., Threnn, Robert H.
Wishnow, Rodney M. (Washington University School of Medicine, St. Louis, Mo.), Jack L. Strominger, Claire H. Birge, and Robert H. Threnn. Biochemical effects of novobiocin on Staphylococcus aureus....
Nucleotide Accumulation Induced in Staphylococcus aureus by Glycine
Strominger, Jack L., Birge, Claire H.
Strominger, Jack L. (Washington University School of Medicine, St. Louis, Mo.), and Claire H. Birge. Nucleotide accumulation induced in Staphylococcus aureus by glycine. J. Bacteriol. 89:1124–1127....
Okazaki, Tuneko, Strominger, Jack L., Okazaki, Reiji
Okazaki, Tuneko (Washington University School of Medicine, St. Louis, Mo.), Jack L. Stominger, and Reiji Okazaki. Thymidine diphosphate-l-rhamnose metabolism in smooth and rough strains of...
EPIMERIZATION OF THYMIDINE DIPHOSPHATE GLUCOSE IN BACTERIAL EXTRACTS
Tinelli, Regina, Michelson, A. M., Strominger, Jack L.
Tinelli, Regina (Washington University School of Medicine, St. Louis, Mo.), A. M. Michelson, and Jack L. Strominger. Epimerization of thymidine diphosphate glucose in bacterial extracts. J....
The mature activating natural killer cell immunologic synapse is formed in distinct stages
Orange, Jordan S., Harris, K. Eliza, Andzelm, Milena M., Valter, Markus M., Geha, Raif S., Strominger, Jack L.
Natural killer (NK) cells form a structure at their interface with a susceptible target cell called the activating NK cell immunologic synapse (NKIS). The mature activating NKIS contains a central...
Kamiryo, Tatsuyuki, Strominger, Jack L.
A group of Escherichia coli mutants which are ampicillin resistant at 32 C and which either are unable to grow or lyse at 42 C has been selected. These mutants have been classified by a number of...
Mechanism of Action and Development of Resistance to a New Amidino Penicillin
Matsuhashi, Sachiko, Kamiryo, Tatsuyuki, Blumberg, Peter M., Linnett, Paul, Willoughby, Eileen, Strominger, Jack L.
The mechanism of killing of Escherichia coli by a novel beta-lactam antibiotic, an amidino penicillin, has been investigated. This compound converts E. coli to relatively stable spherical forms at...
Storm, Daniel R., Blumberg, Peter M., Strominger, Jack L.
An insoluble penicillin derivative, 6-aminopenicillanic acid covalently coupled to Sepharose, was used to investigate the location of the d-alanine carboxypeptidase in the Bacillus subtilis membrane....
Penicillin Binding Components in Bacillius subtilis During Sporulation
Anwar, Rashid A., Blumberg, Peter M., Strominger, Jack L.
The penicillin binding components of sporulating Bacillus subtilis 3610 were compared with those of the vegetative organism. No qualitative difference in binding components was observed. The total...
d-Alanine Carboxypeptidase and Cell Wall Cross-Linking in Bacillus subtilis
Sharpe, Arlene, Blumberg, Peter M., Strominger, Jack L.
Inhibition of d-alanine carboxypeptidase in Bacillus subtilis by 95% caused no measurable change in the degree of cross-linking of the peptidoglycan.
Specificity of a Bacteriolytic Enzyme from Pseudomonas aeruginosa1
Lache, Marvin, Hearn, Walter R., Zyskind, Judith W., Tipper, Donald J., Strominger, Jack L.
A bacteriolytic enzyme isolated from shake-flask cultures of Pseudomonas aeruginosa and capable of lysing cells of Staphylococcus aureus was purified approximately 500-fold by passage through...
Shackelford, Deborah A., Mann, Dean L., Van Rood, Jon J., Ferrara, G. B., Strominger, Jack L.
Some human B-lymphoblastoid cell lines are shown to express at least two types of Ia-like antigens. One antigen is defined by alloantisera to HLA-DR, and the other antigen is defined by alloantisera...
MLL 5 protein forms intranuclear foci, and overexpression inhibits cell cycle progression
Deng, Lih-Wen, Chiu, Isaac, Strominger, Jack L.
MLL5 is a mammalian trithorax group (trx-G) gene identified within chromosome band 7q22, a frequently deleted element found in cytogenetic aberrations of acute myeloid malignancies. MLL5 cDNA was...
Rosenthal, Ken S., Yanovich, Saul, Inbar, Michael, Strominger, Jack L.
Translocation of the hydrocarbon fluorescent probe diphenylhexatriene (DPH) between membranes was studied by fluorescence polarization (P) analysis. First, using a model system, the high P value...
Siebold, Christian, Hansen, Bjarke E., Wyer, Jessica R., Harlos, Karl, Esnouf, Robert E., Svejgaard, Arne, ...
The MHC class II molecule DQ0602 confers strong susceptibility to narcolepsy but dominant protection against type 1 diabetes. The crystal structure of DQ0602 reveals the molecular features underlying...
Partial Purification of Detergent-Soluble HL-A Antigen and Its Cleavage by Papain
Springer, Timothy A., Strominger, Jack L., Mann, Dean
HL-A antigen solubilized with the non-ionic detergent, Brij 99, has been purified to about 50% of homogeneity from a cultured human lymphoblast line. It consists of two nonidentical subunits of...
Cresswell, Peter, Springer, Timothy, Strominger, Jack L., Turner, Mervyn J., Grey, Howard M., Kubo, Ralph T.
A number of immunological methods have been employed to show that the small subunit of HL-A antigens, isolated either after papain treatment or after solubilization with detergent, is identical to...
C55-Isoprenoid Alcohol Phosphokinase: An Extremely Hydrophobic Protein from the Bacterial Membrane
Sandermann, Heinrich, Strominger, Jack L.
C55-isoprenoid alcohol phosphokinase, a butanol-soluble enzyme from the membrane of Staphylococcus aureus, has been purified to homogeneity by the inclusion of organic solvents in all of the...
Blumberg, Peter M., Strominger, Jack L.
The D-alanine carboxypeptidase of Bacillus subtilis is a particulate enzyme that is irreversibly inactivated by penicillins and cephalosporins. However, the lethal concentrations of these antibiotics...
Mechanism of Action of Bacitracin: Complexation with Metal Ion and C55-Isoprenyl Pyrophosphate
Stone, K. John, Strominger, Jack L.
The inhibition by bacitracin of the enzymatic dephosphorylation of C55-isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a...
Blumberg, Peter M., Strominger, Jack L.
An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet...
Rasmussen, James R., Strominger, Jack L.
The penicillin-sensitive D-alanine carboxypeptidases of Bacillus subtilis, Escherichia coli, and Staphylococcus aureus catalyzed the hydrolysis of the D-lactic acid residue from the depsipeptide...
Inhibition of Sterol Biosynthesis by Bacitracin
Stone, K. John, Strominger, Jack L.
Bacitracin is an inhibitor of the biosynthesis of squalene and sterols from mevalonic acid, C5-isopentenyl pyrophosphate, or C15-farnesyl pyrophosphate catalyzed by preparations from rat liver. The...
Gonzalez-Porque, Pedro, Strominger, Jack L.
A cofactor needed for the reduction of CDP-4-keto-6-deoxy-D-glucose to CDP-4-keto-3,6-dideoxy D-glucose has been detected and purified. Its spectra in neutral, acid, and basic solutions, its behavior...
Umbreit, Jay N., Strominger, Jack L.
Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay....
Umbreit, Jay N., Strominger, Jack L.
The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a...
Papain-Solubilized HL-A Antigens from Cultured Human Lymphocytes Contain Two Peptide Fragments
Cresswell, Peter, Turner, Mervyn J., Strominger, Jack L.
HL-A 2 and HL-A 7 antigens have been solubilized from cultured human lymphocytes by papain and purified on a small radioactive scale after formation of immune complexes, or on a large scale by column...
Wedgwood, Josiah F., Warren, Christopher D., Jeanloz, Roger W., Strominger, Jack L.
Fully acetylated chitobiose was treated with phosphoric acid to give a mixture of products from which 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-...
Heterogeneity of HL-A Antigen Preparations Is Due to Variable Sialic Acid Content
Parham, Peter, Humphreys, Robert E., Turner, Mervyn J., Strominger, Jack L.
Purified, papain-solubilized preparations of HL-A antigen from cultured human lymphoblastoid cells (HL-A2 and HL-A7,12 from RPMI 4265 cells and a mixture of HL-A3, W-25, HL-A12, and HL-A27 from IM-1...
Additional Antibiotic Inhibitors of Peptidoglycan Synthesis
Linnett, Paul E., Strominger, Jack L.
Diumycin, janiemycin, nisin, and subtilin inhibited peptidoglycan synthesis catalyzed by particulate enzyme systems from Bacillus stearothermophilus and Escherichia coli. All of these, except for...
Stern, Joel N. H., Illés, Zsolt, Reddy, Jayagopala, Keskin, Derin B., Sheu, Eric, Fridkis-Hareli, Masha, ...
Copolymer 1 [Cop1, glatiramer acetate, Copaxone, poly(Y,E,A,K)n] is widely used in the treatment of relapsing/remitting multiple sclerosis in which it reduces the frequency of relapses by ≈30%. In...
Illés, Zsolt, Stern, Joel N. H., Reddy, Jayagopala, Waldner, Hanspeter, Mycko, Marcin P., Brosnan, Celia F., ...
A humanized mouse bearing the HLA-DR2 (DRA/DRB1*1501) protein associated with multiple sclerosis (MS) and the myelin basic protein (MBP) 85–99-specific HLA-DR2-restricted T cell receptor from an MS...
Wong, Siew Heng, Santambrogio, Laura, Strominger, Jack L.
The passage of dendritic cells (DC) from immature to terminally differentiated antigen-presenting cells is accompanied by numerous morphological, phenotypic, and functional changes. These changes...
Stern, Joel N. H., Illés, Zsolt, Reddy, Jayagopala, Keskin, Derin B., Fridkis-Hareli, Masha, Kuchroo, Vijay K., ...
Myelin basic protein (MBP) is a major candidate autoantigen in multiple sclerosis (MS). Its immunodominant epitope, MBP 85–99, forms a complex with human leukocyte antigen (HLA)-DR2 with which...
Smith, Cassandra E., Eagar, Todd N., Strominger, Jack L., Miller, Stephen D.
The ability of different forms of myelin peptides to induce tolerance for the treatment of preestablished murine experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis,...
Vieillard, Vincent, Strominger, Jack L., Debré, Patrice
HIV infection leads to a state of chronic immune activation and progressive deterioration in immune function, manifested most recognizably by the progressive depletion of CD4+ T cells. A substantial...
Human decidual NK cells form immature activating synapses and are not cytotoxic
Kopcow, Hernan D., Allan, David S. J., Chen, Xi, Rybalov, Basya, Andzelm, Milena M., Ge, Baoxue, ...
In early pregnancy invading fetal trophoblasts encounter abundant maternal decidual natural killer cells (dNK). dNK express perforin, granzymes A and B and the activating receptors NKp30, NKp44,...
Nucleotide Intermediates in the Biosynthesis of Heteropolymeric Polysaccharides
The role of nucleotides as “carriers” of small molecules for biosynthetic reactions is discussed. Following this introduction, the particular problem of nucleotide intermediates in chondroitin...
Nishimura, Hiroyuki, Strominger, Jack L.
Human systemic lupus erythematosus (SLE) and its murine model, MRL lpr/lpr mice, are well known to develop a wide range of symptoms, such as glomerulonephritis, dermatitis, and arthritis, as an...
Gestation stage-dependent mechanisms of invariant natural killer T cell-mediated pregnancy loss
Boyson, Jonathan E., Nagarkatti, Nisha, Nizam, Leena, Exley, Mark A., Strominger, Jack L.
Stimulation of CD1d-restricted semiinvariant natural killer T cells by using the CD1d ligand α-galactosylceramide (αGalCer) induces pregnancy loss in mice through an ill-defined mechanism involving...
Chen, Xi, Allan, David S. J., Krzewski, Konrad, Ge, Baoxue, Kopcow, Hernan, Strominger, Jack L.
Activation of natural killer (NK) cell cytotoxicity requires adhesion and formation of a conjugate with a susceptible target cell, followed by actin polymerization, and polarization of the...
Hou, Runhua, Goloubeva, Olga, Neuberg, Donna S, Strominger, Jack L, Wilson, S Brian
Human invariant natural killer (iNK) T cells expressing an invariant Vα24-Jα15 T-cell receptor (TCR) are thought to be important regulators of autoimmunity and tumour surveillance. Two major...
Keskin, Derin B., Allan, David S. J., Rybalov, Basya, Andzelm, Milena M., Stern, Joel N. H., Kopcow, Hernan D., ...
During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56superbrightCD16−CD9+KIR+ distinct from both subsets of peripheral blood NK...
Chen, Xi, Trivedi, Prachi P., Ge, Baoxue, Krzewski, Konrad, Strominger, Jack L.
Natural killer (NK) cells are components of the innate immune system that recognize and kill tumor or virus-infected target cells through specific NK activating receptor/ligand interactions....
Krzewski, Konrad, Chen, Xi, Orange, Jordan S., Strominger, Jack L.
The tumor natural killer (NK) cell line YTS was used to examine the cytoskeletal rearrangements required for cytolysis. A multiprotein complex weighing ∼1.3 mD and consisting of WASp-interacting...
Myosin IIA is required for cytolytic granule exocytosis in human NK cells
Andzelm, Milena M., Chen, Xi, Krzewski, Konrad, Orange, Jordan S., Strominger, Jack L.
Natural killer (NK) cell cytotoxicity involves the formation of an activating immunological synapse (IS) between the effector and target cell through which granzymes and perforin contained in lytic...
Human Decidual Natural Killer Cells Are a Unique NK Cell Subset with Immunomodulatory Potential
Koopman, Louise A., Kopcow, Hernan D., Rybalov, Basya, Boyson, Jonathan E., Orange, Jordan S., Schatz, Frederick, ...
Natural killer cells constitute 50–90% of lymphocytes in human uterine decidua in early pregnancy. Here, CD56bright uterine decidual NK (dNK) cells were compared with the CD56bright and CD56dim...
CHEMICAL BASIS FOR AN IMMUNOLOGICAL SPECIFICITY OF A STRAIN OF STAPHYLOCOCCUS AUREUS
Juergens, William G., Sanderson, Arnold R., Strominger, Jack L.
Antisera, prepared against formalin-killed cells of Staphylococcus aureus, strain Copenhagen, agglutinated the cell walls of this strain. The agglutination was inhibited by the teichoic acid from the...
THE SMALL SUBUNIT OF HL-A ANTIGENS IS β2-MICROGLOBULIN
Grey, Howard M., Kubo, Ralph T., Colon, Sonia M., Poulik, M. D., Cresswell, Peter, Springer, Timothy, ...
Davis, Daniel M., Mandelboim, Ofer, Luque, Isabel, Baba, Eishi, Boyson, Jonathan, Strominger, Jack L.
Molecular interactions with the extracellular domains of class I major histocompatibility complex proteins are major determinants of immune recognition that have been extensively studied both...
Falk, Kirsten, Rötzschke, Olaf, Santambrogio, Laura, Dorf, Martin E., Brosnan, Celia, Strominger, Jack L.
T cell epitope peptides derived from proteolipid protein (PLP139–151) or myelin basic protein (MBP86–100) induce experimental autoimmune encephalomyelitis (EAE) in “susceptible” strains of...
WIP is essential for lytic granule polarization and NK cell cytotoxicity
Krzewski, Konrad, Chen, Xi, Strominger, Jack L.
Natural killer (NK) cells play important roles in host immunity by killing virus-infected and tumor cells. Killing of the target cell is achieved by formation of an immune synapse and localized...
Li, Changlin, Ge, Baoxue, Nicotra, Matthew, Stern, Joel N. H., Kopcow, Hernan D., Chen, Xi, ...
Interaction of the activating receptor NKG2D with its ligands is a major stimulatory pathway for cytotoxicity of natural killer (NK) cells. Here, the signaling pathway involved after NKG2D ligation...
Stern, Joel N. H., Keskin, Derin B., Zhang, Hong, Lv, HuiJuan, Kato, Zenichiro, Strominger, Jack L.
IL-10-secreting regulatory T cell lines specific to glatiramer acetate [poly(Y,E,A,K)n] or poly(Y,F,A,K)n have been established from the enlarged spleen and lymph nodes that result from copolymer...
Pallandre, Jean R., Krzewski, Konrad, Bedel, Romain, Ryffel, Bernhard, Caignard, Anne, Rohrlich, Pierre Simon, ...
Initial molecular events leading to natural killer lymphocyte (NK) and dendritic cell (DC) interactions are largely unknown. Here, the role of CX3CL1 (fractalkine), a chemokine expressed on mature...
Positioning of autoimmune TCR-Ob.2F3 and TCR-Ob.3D1 on the MBP85–99/HLA-DR2 complex
Kato, Zenichiro, Stern, Joel N. H., Nakamura, Hironori K., Kuwata, Kazuo, Kondo, Naomi, Strominger, Jack L.
Since the first determination of structure of the HLA-A2 complex, >200 MHC/peptide structures have been recorded, whereas the available T cell receptor (TCR)/peptide/MHC complex structures now are
T cell apoptosis at the maternal–fetal interface in early human pregnancy, involvement of galectin-1
Kopcow, Hernan D., Rosetti, Florencia, Leung, Yiuka, Allan, David S. J., Kutok, Jeffrey L., Strominger, Jack L.
The human fetus is not rejected by the maternal immune system despite expressing paternal antigens. Natural killer cells, the major lymphocyte population of the human decidua (dNKs), express genes...
Zhang, Hong, Stern, Joel N. H., Strominger, Jack L.
The T cell receptors from the regulatory IL-10-secreting T cell line induced by the random amino acid copolymers poly(F,Y,A,K,)n in SJL mice (H-2s) have been characterized, cloned, sequenced and...
Li, Changlin, Houser, Brandy L., Nicotra, Matthew L., Strominger, Jack L.
Human decidual CD14+ macrophages and CD56+ NK cells were isolated from material obtained after first-trimester pregnancy terminations. Each cell type expressed a specific surface receptor for...