Jack R. Bennink

The TL MHC class Ib molecule has only marginal effects on the activation, survival and trafficking of mouse small intestinal intraepithelial lymphocytes (2004)

Pardigon, Nathalie, Darche, Sylvie, Kelsall, Brian, Bennink, Jack R., Yewdell, Jonathan W.

Thymus leukemia antigen (TL) is an MHC class Ib molecule that is highly conserved in rats and mice with no obvious human homolog. TL is expressed in mouse small intestinal epithelial cells and is...

Multiple antigen-specific processing pathways for activating naive CD8+ T cells in vivo (2001)

Norbury, Christopher C., Princiotta, Michael F., Bacik, Igor, Brutkiewicz, Randy R., Wood, Phillip, Elliott, Tim, ...

Current knowledge of the processing of viral Ags into MHC class I-associated ligands is based almost completely on in vitro studies using nonprofessional APCs (pAPCs). This is two steps removed from...

Cells adapted to the proteasome inhibitor 4-hydroxy- 5-iodo-3-nitrophenylacetyl-Leu-Leu-leucinal-vinyl sulfone require enzymatically active proteasomes for continued survival

Princiotta, Michael F., Schubert, Ulrich, Chen, Weisan, Bennink, Jack R., Myung, Jayhyuk, Crews, Craig M., ...

The proteasome is the primary protease used by cells for degrading proteins and generating peptide ligands for class I molecules of the major histocompatibility complex. Based on the properties of...

Direct delivery of exogenous MHC class I molecule-binding oligopeptides to the endoplasmic reticulum of viable cells

Day, Patricia M., Yewdell, Jonathan W., Porgador, Angel, Germain, Ronald N., Bennink, Jack R.

After brief incubation of cells with fluorescein-conjugated peptides that bind major histocompatibility complex (MHC) class I molecules, peptides were detected within the endoplasmic reticulum (ER)...

Proteasome inhibition interferes with Gag polyprotein processing, release, and maturation of HIV-1 and HIV-2

Schubert, Ulrich, Ott, David E., Chertova, Elena N., Welker, Reinhold, Tessmer, Uwe, Princiotta, Michael F., ...

Retrovirus assembly and maturation involve folding and transport of viral proteins to the virus assembly site followed by subsequent proteolytic cleavage of the Gag polyprotein within the nascent...

The Export of Major Histocompatibility Complex Class I Molecules from the Endoplasmic Reticulum of Rat Brown Adipose Cells Is Acutely Stimulated by Insulin

Malide, Daniela, Yewdell, Jonathan W., Bennink, Jack R., Cushman, Samuel W.

Major histocompatibility complex class I (MHC-I) molecules have been implicated in several nonimmunological functions including the regulation and intracellular trafficking of the insulin-responsive...

CD4 Glycoprotein Degradation Induced by Human Immunodeficiency Virus Type 1 Vpu Protein Requires the Function of Proteasomes and the Ubiquitin-Conjugating Pathway

Schubert, Ulrich, Antón, Luis C., Bačík, Igor, Cox, Josephine H., Bour, Stéphane, Bennink, Jack R., ...

The human immunodeficiency virus type 1 (HIV-1) vpu gene encodes a type I anchored integral membrane phosphoprotein with two independent functions. First, it regulates virus release from a...

Human Cytomegalovirus Protein US2 Interferes with the Expression of Human HFE, a Nonclassical Class I Major Histocompatibility Complex Molecule That Regulates Iron Homeostasis

Ben-Arieh, Sayeh Vahdati, Zimerman, Baruch, Smorodinsky, Nechama I., Yaacubovicz, Margalit, Schechter, Chana, Bacik, Igor, ...

HFE is a nonclassical class I major histocompatibility complex (MHC) molecule that is mutated in the autosomal recessive iron overload disease hereditary hemochromatosis. There is evidence linking...

Cross-Reactivity between Hepatitis C Virus and Influenza A Virus Determinant-Specific Cytotoxic T Cells

Wedemeyer, Heiner, Mizukoshi, Eishiro, Davis, Anthony R., Bennink, Jack R., Rehermann, Barbara

The cellular immune response contributes to viral clearance as well as to liver injury in acute and chronic hepatitis C virus (HCV) infection. An immunodominant determinant frequently recognized by...

An Endoplasmic Reticulum-Targeting Signal Sequence Enhances the Immunogenicity of an Immunorecessive Simian Virus 40 Large T Antigen Cytotoxic T-Lymphocyte Epitope

Fu, Tong-Ming, Mylin, Lawrence M., Schell, Todd D., Bacik, Igor, Russ, Gustav, Yewdell, Jonathan W., ...

An immunological hierarchy among three H-2Db-restricted cytotoxic T lymphocyte (CTL) determinants in simian virus 40 (SV40) large T antigen (Tag) was described previously: determinants I and II/III...

Mice Deficient in Perforin, CD4+ T Cells, or CD28-Mediated Signaling Maintain the Typical Immunodominance Hierarchies of CD8+ T-Cell Responses to Influenza Virus

Chen, Weisan, Bennink, Jack R., Morton, Phillip A., Yewdell, Jonathan W.

CD8 T-cell (TCD8+) responses elicited by viral infection demonstrate the phenomenon of immunodominance: the numbers of TCD8+ responding to different viral peptides vary over a wide range in a...

Heat-Aggregated Noninfectious Influenza Virus Induces a More Balanced CD8+-T-Lymphocyte Immunodominance Hierarchy Than Infectious Virus

Cho, Yunjung, Basta, Sameh, Chen, Weisan, Bennink, Jack R., Yewdell, Jonathan W.

CD8+-T-cell (TCD8+) responses to infectious viruses are characterized by an immunodominance hierarchy in which the majority of TCD8+ respond to one or a few immunodominant determinants, with a...

The Influenza A Virus PB1-F2 Protein Targets the Inner Mitochondrial Membrane via a Predicted Basic Amphipathic Helix That Disrupts Mitochondrial Function

Gibbs, James S., Malide, Daniela, Hornung, Felicita, Bennink, Jack R., Yewdell, Jonathan W.

The 11th influenza A virus gene product is an 87-amino-acid protein provisionally named PB1-F2 (because it is encoded by an open reading frame overlapping the PB1 open reading frame). A significant...

HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation

Oseroff, Carla, Kos, Ferdynand, Bui, Huynh-Hoa, Peters, Bjoern, Pasquetto, Valerie, Glenn, Jean, ...

We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from...

Poxvirus CD8+ T-Cell Determinants and Cross-Reactivity in BALB/c Mice

Tscharke, David C., Woo, Wai-Ping, Sakala, Isaac G., Sidney, John, Sette, Alessandro, Moss, Denis J., ...

Mouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and...

Cells adapted to the proteasome inhibitor 4-hydroxy- 5-iodo-3-nitrophenylacetyl-Leu-Leu-leucinal-vinyl sulfone require enzymatically active proteasomes for continued survival

Princiotta, Michael F., Schubert, Ulrich, Chen, Weisan, Bennink, Jack R., Myung, Jayhyuk, Crews, Craig M., ...

The proteasome is the primary protease used by cells for degrading proteins and generating peptide ligands for class I molecules of the major histocompatibility complex. Based on the properties of...

Direct delivery of exogenous MHC class I molecule-binding oligopeptides to the endoplasmic reticulum of viable cells

Day, Patricia M., Yewdell, Jonathan W., Porgador, Angel, Germain, Ronald N., Bennink, Jack R.

After brief incubation of cells with fluorescein-conjugated peptides that bind major histocompatibility complex (MHC) class I molecules, peptides were detected within the endoplasmic reticulum (ER)...

Proteasome inhibition interferes with Gag polyprotein processing, release, and maturation of HIV-1 and HIV-2

Schubert, Ulrich, Ott, David E., Chertova, Elena N., Welker, Reinhold, Tessmer, Uwe, Princiotta, Michael F., ...

Retrovirus assembly and maturation involve folding and transport of viral proteins to the virus assembly site followed by subsequent proteolytic cleavage of the Gag polyprotein within the nascent...

The Export of Major Histocompatibility Complex Class I Molecules from the Endoplasmic Reticulum of Rat Brown Adipose Cells Is Acutely Stimulated by Insulin

Malide, Daniela, Yewdell, Jonathan W., Bennink, Jack R., Cushman, Samuel W.

Major histocompatibility complex class I (MHC-I) molecules have been implicated in several nonimmunological functions including the regulation and intracellular trafficking of the insulin-responsive...

CD4 Glycoprotein Degradation Induced by Human Immunodeficiency Virus Type 1 Vpu Protein Requires the Function of Proteasomes and the Ubiquitin-Conjugating Pathway

Schubert, Ulrich, Antón, Luis C., Bačík, Igor, Cox, Josephine H., Bour, Stéphane, Bennink, Jack R., ...

The human immunodeficiency virus type 1 (HIV-1) vpu gene encodes a type I anchored integral membrane phosphoprotein with two independent functions. First, it regulates virus release from a...

Human Cytomegalovirus Protein US2 Interferes with the Expression of Human HFE, a Nonclassical Class I Major Histocompatibility Complex Molecule That Regulates Iron Homeostasis

Ben-Arieh, Sayeh Vahdati, Zimerman, Baruch, Smorodinsky, Nechama I., Yaacubovicz, Margalit, Schechter, Chana, Bacik, Igor, ...

HFE is a nonclassical class I major histocompatibility complex (MHC) molecule that is mutated in the autosomal recessive iron overload disease hereditary hemochromatosis. There is evidence linking...

Cross-Reactivity between Hepatitis C Virus and Influenza A Virus Determinant-Specific Cytotoxic T Cells

Wedemeyer, Heiner, Mizukoshi, Eishiro, Davis, Anthony R., Bennink, Jack R., Rehermann, Barbara

The cellular immune response contributes to viral clearance as well as to liver injury in acute and chronic hepatitis C virus (HCV) infection. An immunodominant determinant frequently recognized by...

An Endoplasmic Reticulum-Targeting Signal Sequence Enhances the Immunogenicity of an Immunorecessive Simian Virus 40 Large T Antigen Cytotoxic T-Lymphocyte Epitope

Fu, Tong-Ming, Mylin, Lawrence M., Schell, Todd D., Bacik, Igor, Russ, Gustav, Yewdell, Jonathan W., ...

An immunological hierarchy among three H-2Db-restricted cytotoxic T lymphocyte (CTL) determinants in simian virus 40 (SV40) large T antigen (Tag) was described previously: determinants I and II/III...

Mice Deficient in Perforin, CD4+ T Cells, or CD28-Mediated Signaling Maintain the Typical Immunodominance Hierarchies of CD8+ T-Cell Responses to Influenza Virus

Chen, Weisan, Bennink, Jack R., Morton, Phillip A., Yewdell, Jonathan W.

CD8 T-cell (TCD8+) responses elicited by viral infection demonstrate the phenomenon of immunodominance: the numbers of TCD8+ responding to different viral peptides vary over a wide range in a...

Heat-Aggregated Noninfectious Influenza Virus Induces a More Balanced CD8+-T-Lymphocyte Immunodominance Hierarchy Than Infectious Virus

Cho, Yunjung, Basta, Sameh, Chen, Weisan, Bennink, Jack R., Yewdell, Jonathan W.

CD8+-T-cell (TCD8+) responses to infectious viruses are characterized by an immunodominance hierarchy in which the majority of TCD8+ respond to one or a few immunodominant determinants, with a...

The Influenza A Virus PB1-F2 Protein Targets the Inner Mitochondrial Membrane via a Predicted Basic Amphipathic Helix That Disrupts Mitochondrial Function

Gibbs, James S., Malide, Daniela, Hornung, Felicita, Bennink, Jack R., Yewdell, Jonathan W.

The 11th influenza A virus gene product is an 87-amino-acid protein provisionally named PB1-F2 (because it is encoded by an open reading frame overlapping the PB1 open reading frame). A significant...

HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation

Oseroff, Carla, Kos, Ferdynand, Bui, Huynh-Hoa, Peters, Bjoern, Pasquetto, Valerie, Glenn, Jean, ...

We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from...

Poxvirus CD8+ T-Cell Determinants and Cross-Reactivity in BALB/c Mice

Tscharke, David C., Woo, Wai-Ping, Sakala, Isaac G., Sidney, John, Sette, Alessandro, Moss, Denis J., ...

Mouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and...

Viral Alteration of Cellular Translational Machinery Increases Defective Ribosomal Products▿

Berglund, Peter, Finzi, Diana, Bennink, Jack R., Yewdell, Jonathan W.

Here we show that cells expressing genes inserted into Semliki Forest virus (SFV) vectors generate a large fraction of defective ribosomal products (DRiPs) due to frequent initiation on downstream...

Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines

Tscharke, David C., Karupiah, Gunasegaran, Zhou, Jie, Palmore, Tara, Irvine, Kari R., Haeryfar, S.M. Mansour, ...

The large size of poxvirus genomes has stymied attempts to identify determinants recognized by CD8+ T cells and greatly impeded development of mouse smallpox vaccination models. Here, we use a...

Modification of Cysteine Residues In Vitro and In Vivo Affects the Immunogenicity and Antigenicity of Major Histocompatibility Complex Class I–restricted Viral Determinants

Chen, Weisan, Yewdell, Jonathan W., Levine, Rodney L., Bennink, Jack R.

In studying the subdominant status of two cysteine-containing influenza virus nuclear protein (NP) determinants (NP39–47 and NP218–226) restricted by H-2Kd, we found that the antigenicity of...

Immunoproteasomes Shape Immunodominance Hierarchies of Antiviral Cd8+ T Cells at the Levels of T Cell Repertoire and Presentation of Viral Antigens

Chen, Weisan, Norbury, Christopher C., Cho, Yunjung, Yewdell, Jonathan W., Bennink, Jack R.

Vertebrates express three cytokine-inducible proteasome subunits that are incorporated in the place of their constitutively synthesized counterparts. There is increasing evidence that the set of...

The Human Immunodeficiency Virus Type 1 (HIV-1) Vpu Protein Interferes with an Early Step in the Biosynthesis of Major Histocompatibility Complex (MHC) Class I Molecules

Kerkau, Thomas, Bacik, Igor, Bennink, Jack R., Yewdell, Jonathan W., Hünig, Thomas, Schimpl, Anneliese, ...

The human immunodeficiency virus type 1 (HIV-1) vpu gene encodes a small integral membrane phosphoprotein with two established functions: degradation of the viral coreceptor CD4 in the endoplasmic...

Introduction of a Glycosylation Site into a Secreted Protein Provides Evidence for an Alternative Antigen Processing Pathway: Transport of Precursors of Major Histocompatability Complex Class I–Restricted Peptides from the Endoplasmic Reticulum to the Cytosol

Bačík, Igor, Snyder, Heidi Link, Antón, Luis C., Russ, Gustav, Chen, Weisan, Bennink, Jack R., ...

We found that the presentation of a H-2Kd-restricted determinant from influenza virus nucleoprotein (NP) to T cells is strictly dependent on expression of the transporter associated with antigen...

Two Novel Routes of Transporter Associated with Antigen Processing (TAP)-independent Major Histocompatibility Complex Class I Antigen Processing

Snyder, Heidi Link, Bačík, Igor, Bennink, Jack R., Kearns, Grainne, Behrens, Timothy W., Bächi, Thomas, ...

Jaw1 is an endoplasmic reticulum (ER) resident protein representative of a class of proteins post translationally inserted into membranes via a type II membrane anchor (cytosolic NH2 domain, lumenal...

Intracellular Localization of Proteasomal Degradation of a Viral Antigen

Antón, Luis C., Schubert, Ulrich, Bacík, Igor, Princiotta, Michael F., Wearsch, Pamela A., Gibbs, James, ...

To better understand proteasomal degradation of nuclear proteins and viral antigens we studied mutated forms of influenza virus nucleoprotein (NP) that misfold and are rapidly degraded by...

Sympathetic nervous system control of anti-influenza CD8+ T cell responses

Grebe, Kristie M., Hickman, Heather D., Irvine, Kari R., Takeda, Kazuyo, Bennink, Jack R., Yewdell, Jonathan W.

Despite the longstanding appreciation of communication between the nervous and the immune systems, the nature and significance of these interactions to immunity remain enigmatic. Here, we show that...