Jacob Dankert

Antibiotic guidelines and antibiotic use in adult bacterial meningitis in The Netherlands (2002)

Van De Beek, Diederik, De Gans, Jan, Spanjaard, Lodewijk, Vermeulen, Marinus, Dankert, Jacob

In The Netherlands, national guidelines for the treatment of adult patients with bacterial meningitis were introduced in October 1997. In 1998 we began a prospective, nationwide study to evaluate the...

Thrombocidins, Microbicidal Proteins from Human Blood Platelets, Are C-terminal Deletion Products of CXC Chemokines (2000)

Kuijpers, Alma J., Ehlert, Jan E., Brandt, Ernst, Poolman, Bert, Fang, Gang, Veelen, Peter A. Van, ...

Antibacterial proteins are components of the innate immune system found in many organisms and produced by a variety of cell types. Human blood platelets contain a number of antibacterial proteins in...

Broad-Host-Range Shuttle Vectors for Screening of Regulated Promoter Activity in Viridans Group Streptococci: Isolation of a pH-Regulated Promoter (2000)

Vriesema, Aldwin J.M., Brinkman, René, Kok, Jan, Dankert, Jacob, Zaat, Sebastian A.J.

Viridans group streptococci are major constituents of the normal human oral flora and are also identified as the predominant pathogenic bacteria in native valve infective endocarditis. Little...

Thrombocidins, Microbicidal Proteins from Human Blood Platelets, Are C-terminal Deletion Products of CXC Chemokines (2000)

Krijgsveld, Jeroen, Zaat, Sebastian A.J., Meeldijk, Jan, Veelen, Peter A. Van, Fang, Gang, Poolman, Bert, ...

Antibacterial proteins are components of the innate immune system found in many organisms and produced by a variety of cell types. Human blood platelets contain a number of antibacterial proteins in...

Antibacterial activity of antibiotic-soaked polyvinylpyrrolidone-grafted silicon elastomer hydrocephalus shunts (2000)

Boelens, Jaap J., Tan, Wee-Fu, Dankert, Jacob, Zaat, Sebastian A. J.

If shunts, inserted for the relief of hydrocephalus, are pretreated with antimicrobials, the incidence of shunt-associated infections (SAI) may be reduced. The duration of the antibacterial activity...

Role of platelets in early pathogenesis of viridans group streptococcal endocarditis: a study on thrombodefensins. A study in which penicillin tolerance is shown to be associated with increased virulence. (1988)

Dankert, Jacob

In the pathogenesis of infective endocarditis the adherence of circulating bacteria to a preexisting cardiac vegetation (VG) and the survival of bacteria adherent to VG's are major events. One...

Tick-Borne Relapsing Fever Imported from West Africa: Diagnosis by Quantitative Buffy Coat Analysis and In Vitro Culture of Borrelia crocidurae

Van Dam, Alje P., Van Gool, Tom, Dankert, Jacob

West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia...

Phenotypic and Genetic Characterization of a Novel Borrelia burgdorferi Sensu Lato Isolate from a Patient with Lyme Borreliosis

Wang, Guiqing, Van Dam, Alje P., Dankert, Jacob

Borrelia burgdorferi sensu lato A14S was cultured from a skin biopsy specimen of a patient with erythema migrans in The Netherlands. This isolate had a unique DNA fingerprint pattern compared to 135...

Genotyping of Mycoplasma pneumoniae Clinical Isolates Reveals Eight P1 Subtypes within Two Genomic Groups

Dorigo-Zetsma, J. Wendelien, Dankert, Jacob, Zaat, Sebastian A. J.

Three methods for genotyping of Mycoplasma pneumoniae clinical isolates were applied to 2 reference strains and 21 clinical isolates. By a modified restriction fragment length polymorphism (RFLP)...

Rapid Diagnosis of Legionnaires' Disease Using an Immunochromatographic Assay for Legionella pneumophila Serogroup 1 Antigen in Urine during an Outbreak in The Netherlands

Wever, Peter C., Yzerman, Ed P. F., Kuijper, Ed J., Speelman, Peter, Dankert, Jacob

A new immunochromatographic assay for rapid qualitative detection of Legionella pneumophila serogroup 1 antigen in urine specimens was used during an outbreak of legionellosis in The Netherlands. The...

PCR Assessment of Chlamydia trachomatis Infection of Semen Specimens Processed for Artificial Insemination

Pannekoek, Yvonne, Westenberg, Steven M., De Vries, Jan, Repping, Sjoerd, Spanjaard, Lodewijk, Eijk, Paul P., ...

In order to ascertain the microbiological quality of stored semen specimens processed for artificial insemination by a donor (AID), we developed a PCR assay targeting the chlamydial plasmid to detect...

Genetic Relatedness within Serotypes of Penicillin-Susceptible Streptococcus pneumoniae Isolates

Overweg, Karin, Bogaert, Debby, Sluijter, Marcel, Yother, Janet, Dankert, Jacob, De Groot, Ronald, ...

The molecular epidemiological characteristics of all Streptococcus pneumoniae strains isolated in a nationwide manner from patients with meningitis in The Netherlands in 1994 were investigated....

Apparent Culture-Negative Prosthetic Valve Endocarditis Caused by Peptostreptococcus magnus

Van Der Vorm, Eric R., Dondorp, Arjen M., Van Ketel, Ruud J., Dankert, Jacob

In two patients with prosthetic valve endocarditis due to Peptostreptococcus magnus, blood cultures in the BacT/Alert and BACTEC 9240 systems were signal negative. The capability of the BacT/Alert...

Molecular Typing of Borrelia burgdorferi Sensu Lato: Taxonomic, Epidemiological, and Clinical Implications

Wang, Guiqing, Van Dam, Alje P., Schwartz, Ira, Dankert, Jacob

Borrelia burgdorferi sensu lato, the spirochete that causes human Lyme borreliosis (LB), is a genetically and phenotypically divergent species. In the past several years, various molecular approaches...

Variation in the Composition and Pore Function of Major Outer Membrane Pore Protein P2 of Haemophilus influenzae from Cystic Fibrosis Patients

Regelink, Annette G., Dahan, David, Möller, Lieke V. M., Coulton, James W., Eijk, Paul, Van Ulsen, Peter, ...

We investigated the relationship between susceptibility to β-lactam antibiotics and variation in the major outer membrane protein P2 (OmpP2; also called porin) of persistent nonencapsulated...

Broad-Host-Range Shuttle Vectors for Screening of Regulated Promoter Activity in Viridans Group Streptococci: Isolation of a pH-Regulated Promoter

Vriesema, Aldwin J. M., Brinkman, René, Kok, Jan, Dankert, Jacob, Zaat, Sebastian A. J.

Viridans group streptococci are major constituents of the normal human oral flora and are also identified as the predominant pathogenic bacteria in native valve infective endocarditis. Little...

Antigenic Variation of the Class I Outer Membrane Protein in Hyperendemic Neisseria meningitidis Strains in The Netherlands

Bart, Aldert, Dankert, Jacob, Van Der Ende, Arie

Since 1980, the number of cases of meningococcal disease caused by serogroup B isolates with the P1.4 serosubtype has greatly increased in The Netherlands. Screening for this serosubtype in the...

A Shift from Oral to Blood pH Is a Stimulus for Adaptive Gene Expression of Streptococcus gordonii CH1 and Induces Protection against Oxidative Stress and Enhanced Bacterial Growth by Expression of msrA

Vriesema, Aldwin J. M., Dankert, Jacob, Zaat, Sebastian A. J.

Viridans group streptococci (VS) from the oral cavity entering the bloodstream may initiate infective endocarditis (IE). We aimed to identify genes expressed in response to a pH increase from...

Altered Gene Expression in Staphylococcus aureus upon Interaction with Human Endothelial Cells

Vriesema, Aldwin J. M., Beekhuizen, Henry, Hamdi, Mohamed, Soufan, Alexandre, Lammers, Aart, Willekens, Ben, ...

Staphylococcus aureus is isolated from a substantial number of patients with infective endocarditis who are not known to have predisposing heart abnormalities. It has been suggested that the...

Multiple Mechanisms of Phase Variation of PorA in Neisseria meningitidis

Van Der Ende, Arie, Hopman, Carla T. P., Dankert, Jacob

Previously, we reported that PorA expression in Neisseria meningitidis is modulated by variation in the length of the homopolymeric tract of guanidine residues between the −35 and −10 regions of...

Interleukin-1 Receptor Type I Gene-Deficient Mice Are Less Susceptible to Staphylococcus epidermidis Biomaterial-Associated Infection than Are Wild-Type Mice

Boelens, Jaap Jan, Van Der Poll, Tom, Zaat, Sebastian A. J., Weening, Jan J., Dankert, Jacob

Elevated concentrations of interleukin-1 (IL-1) were found in tissue surrounding biomaterials infected with Staphylococcus epidermidis. To determine the role of IL-1 in biomaterial-associated...

NmeSI Restriction-Modification System Identified by Representational Difference Analysis of a Hypervirulent Neisseria meningitidis Strain

Bart, Aldert, Pannekoek, Yvonne, Dankert, Jacob, Van Der Ende, Arie

Neisseria meningitidis is a gram-negative bacterium that may cause meningitis, sepsis, or both. The increase in the incidence of meningococcal disease in various countries in the past 2 decades is...

Cloning of Genes of Nontypeable Haemophilus influenzae Involved in Penetration between Human Lung Epithelial Cells

Van Schilfgaarde, Muriel, Van Ulsen, Peter, Van Der Steeg, Wim, Winter, Victor, Eijk, Paul, Everts, Vincent, ...

Haemophilus influenzae penetrates between epithelial cells via an unknown mechanism. A chromosomal library of nonencapsulated H. influenzae strain A960053 DNA was constructed in Escherichia coli...

Characterization of Adherence of Nontypeable Haemophilus influenzae to Human Epithelial Cells

Van Schilfgaarde, Muriel, Van Ulsen, Peter, Eijk, Paul, Brand, Michiel, Stam, Martin, Kouame, Jamal, ...

The adherence of 58 nontypeable Haemophilus influenzae isolates obtained from patients with otitis media or chronic obstructive pulmonary disease (COPD) and obtained from the throats of healthy...

Normal IncA Expression and Fusogenicity of Inclusions in Chlamydia trachomatis Isolates with the incA I47T Mutation

Pannekoek, Yvonne, Van Der Ende, Arie, Eijk, Paul P., Van Marle, Jan, De Witte, Moniek A., Ossewaarde, Jacobus M., ...

To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were...

Mycoplasma pneumoniae P1 Type 1- and Type 2-Specific Sequences within the P1 Cytadhesin Gene of Individual Strains

Dorigo-Zetsma, J. Wendelien, Wilbrink, Berry, Dankert, Jacob, Zaat, Sebastian A. J.

Mycoplasma pneumoniae strains traditionally are divided into two types, based on sequence variation in the P1 gene. Recently, however, we have identified 8 P1 subtypes by restriction fragment length...

Molecular Typing of Borrelia burgdorferi Sensu Lato by Randomly Amplified Polymorphic DNA Fingerprinting Analysis

Wang, Guiqing, Van Dam, Alje P., Spanjaard, Lodewijk, Dankert, Jacob

To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from...

Randomly Amplified Polymorphic DNA Genotyping of Serogroup A Meningococci Yields Results Similar to Those Obtained by Multilocus Enzyme Electrophoresis and Reveals New Genotypes

Bart, Aldert, Schuurman, Ilse G. A., Achtman, Mark, Caugant, Dominique A., Dankert, Jacob, Van Der Ende, Arie

Randomly amplified polymorphic DNA (RAPD) genotyping was applied to one representative strain of each of the 84 electrophoretic types (ETs) of Neisseria meningitidis serogroup A previously defined by...

Antimicrobial Susceptibility of Haemophilus influenzae in the Respiratory Tracts of Patients with Cystic Fibrosis

Möller, Lieke V. M., Regelink, Annette G., Grasselier, Henny, Van Alphen, Loek, Dankert, Jacob

We analyzed the antimicrobial susceptibilities of Haemophilus influenzae isolates from 157 sputum specimens prospectively collected from 39 cystic fibrosis (CF) patients during a 2-year study. These...

cagA-Positive Helicobacter pylori Populations in China and The Netherlands Are Distinct

Van Der Ende, Arie, Pan, Zhi-Jun, Bart, Aldert, Feller, Monique, Xiao, Shu-Dong, ...

The aim of this research was to study whether and to what extent Chinese cagA-positive Helicobacter pylori isolates differ from those in The Netherlands. Analysis of random amplified polymorphic DNA...

Assessment of Clarithromycin-Resistant Helicobacter pylori among Patients in Shanghai and Guangzhou, China, by Primer-Mismatch PCR

Pan, Zhi-Jun, Su, Wan-Wen, Tytgat, Guido N. J., Dankert, Jacob, Van Der Ende, Arie

Of 96 Helicobacter pylori isolates from patients in Shanghai and Guangzhou, China, 5 had the A2143G 23S rRNA mutation as determined by primer-mismatch PCR and were resistant to clarithromycin by the...

Sensitivity of Three Urinary Antigen Tests Associated with Clinical Severity in a Large Outbreak of Legionnaires' Disease in The Netherlands

Yzerman, Ed P. F., Boer, Jeroen W. Den, Lettinga, Kamilla D., Schellekens, Joop, Dankert, Jacob, Peeters, Marcel

In 1999 an outbreak involving 188 patients with Legionnaires' disease (LD) occurred among visitors to a flower show in the Netherlands. Two enzyme immunoassays (Binax and Biotest) and one...

Detection of Imported Malaria with the Cell-Dyn 4000 Hematology Analyzer

Wever, Peter C., Henskens, Yvonne M. C., Kager, Piet A., Dankert, Jacob, Van Gool, Tom

The sensitivity and specificity of the Cell-Dyn 4000 hematology analyzer in the diagnosis of imported malaria were studied with samples from patients in an academic hospital setting. The performance...

Detection of a Point Mutation Associated with High-Level Isoniazid Resistance in Mycobacterium tuberculosis by Using Real-Time PCR Technology with 3′-Minor Groove Binder-DNA Probes

Van Doorn, H. Rogier, Claas, Eric C. J., Templeton, Kate E., Te Koppele Vije, Arianne, De Jong, Menno D., ...

Tuberculosis remains one of the leading infectious causes of death worldwide. The emergence of drug-resistant strains of Mycobacterium tuberculosis is a serious public health threat. Resistance to...

Major Histocompatibility Complex Class I Peptide Presentation after Salmonella enterica Serovar Typhimurium Infection Assessed via Stable Isotope Tagging of the B27-Presented Peptide Repertoire

Ringrose, Jeffrey H., Meiring, Hugo D., Speijer, Dave, Feltkamp, Theodorus E. W., ...

Reactive arthritis (ReA) induced by infection with several gram-negative bacteria is strongly associated with expression of the major histocompatibility complex class I molecule HLA-B27. It is...

Interleukin 1α Increases the Susceptibility of Rabbits to Experimental Viridans Streptococcal Endocarditis

Dankert, Jacob, Van Der Werff, Janneke, Joldersma, Willem, Zaat, Sebastian A. J.

Major predisposing conditions for infective endocarditis (IE) are the presence of a cardiac platelet-fibrin vegetation and of circulating bacteria with relatively low susceptibility to microbicidal...

Tick-Borne Relapsing Fever Imported from West Africa: Diagnosis by Quantitative Buffy Coat Analysis and In Vitro Culture of Borrelia crocidurae

Van Dam, Alje P., Van Gool, Tom, Dankert, Jacob

West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia...

Phenotypic and Genetic Characterization of a Novel Borrelia burgdorferi Sensu Lato Isolate from a Patient with Lyme Borreliosis

Wang, Guiqing, Van Dam, Alje P., Dankert, Jacob

Borrelia burgdorferi sensu lato A14S was cultured from a skin biopsy specimen of a patient with erythema migrans in The Netherlands. This isolate had a unique DNA fingerprint pattern compared to 135...

Genotyping of Mycoplasma pneumoniae Clinical Isolates Reveals Eight P1 Subtypes within Two Genomic Groups

Dorigo-Zetsma, J. Wendelien, Dankert, Jacob, Zaat, Sebastian A. J.

Three methods for genotyping of Mycoplasma pneumoniae clinical isolates were applied to 2 reference strains and 21 clinical isolates. By a modified restriction fragment length polymorphism (RFLP)...

Rapid Diagnosis of Legionnaires' Disease Using an Immunochromatographic Assay for Legionella pneumophila Serogroup 1 Antigen in Urine during an Outbreak in The Netherlands

Wever, Peter C., Yzerman, Ed P. F., Kuijper, Ed J., Speelman, Peter, Dankert, Jacob

A new immunochromatographic assay for rapid qualitative detection of Legionella pneumophila serogroup 1 antigen in urine specimens was used during an outbreak of legionellosis in The Netherlands. The...

PCR Assessment of Chlamydia trachomatis Infection of Semen Specimens Processed for Artificial Insemination

Pannekoek, Yvonne, Westenberg, Steven M., De Vries, Jan, Repping, Sjoerd, Spanjaard, Lodewijk, Eijk, Paul P., ...

In order to ascertain the microbiological quality of stored semen specimens processed for artificial insemination by a donor (AID), we developed a PCR assay targeting the chlamydial plasmid to detect...

Genetic Relatedness within Serotypes of Penicillin-Susceptible Streptococcus pneumoniae Isolates

Overweg, Karin, Bogaert, Debby, Sluijter, Marcel, Yother, Janet, Dankert, Jacob, De Groot, Ronald, ...

The molecular epidemiological characteristics of all Streptococcus pneumoniae strains isolated in a nationwide manner from patients with meningitis in The Netherlands in 1994 were investigated....

Apparent Culture-Negative Prosthetic Valve Endocarditis Caused by Peptostreptococcus magnus

Van Der Vorm, Eric R., Dondorp, Arjen M., Van Ketel, Ruud J., Dankert, Jacob

In two patients with prosthetic valve endocarditis due to Peptostreptococcus magnus, blood cultures in the BacT/Alert and BACTEC 9240 systems were signal negative. The capability of the BacT/Alert...

Molecular Typing of Borrelia burgdorferi Sensu Lato: Taxonomic, Epidemiological, and Clinical Implications

Wang, Guiqing, Van Dam, Alje P., Schwartz, Ira, Dankert, Jacob

Borrelia burgdorferi sensu lato, the spirochete that causes human Lyme borreliosis (LB), is a genetically and phenotypically divergent species. In the past several years, various molecular approaches...

Variation in the Composition and Pore Function of Major Outer Membrane Pore Protein P2 of Haemophilus influenzae from Cystic Fibrosis Patients

Regelink, Annette G., Dahan, David, Möller, Lieke V. M., Coulton, James W., Eijk, Paul, Van Ulsen, Peter, ...

We investigated the relationship between susceptibility to β-lactam antibiotics and variation in the major outer membrane protein P2 (OmpP2; also called porin) of persistent nonencapsulated...

Broad-Host-Range Shuttle Vectors for Screening of Regulated Promoter Activity in Viridans Group Streptococci: Isolation of a pH-Regulated Promoter

Vriesema, Aldwin J. M., Brinkman, René, Kok, Jan, Dankert, Jacob, Zaat, Sebastian A. J.

Viridans group streptococci are major constituents of the normal human oral flora and are also identified as the predominant pathogenic bacteria in native valve infective endocarditis. Little...

Antigenic Variation of the Class I Outer Membrane Protein in Hyperendemic Neisseria meningitidis Strains in The Netherlands

Bart, Aldert, Dankert, Jacob, Van Der Ende, Arie

Since 1980, the number of cases of meningococcal disease caused by serogroup B isolates with the P1.4 serosubtype has greatly increased in The Netherlands. Screening for this serosubtype in the...

A Shift from Oral to Blood pH Is a Stimulus for Adaptive Gene Expression of Streptococcus gordonii CH1 and Induces Protection against Oxidative Stress and Enhanced Bacterial Growth by Expression of msrA

Vriesema, Aldwin J. M., Dankert, Jacob, Zaat, Sebastian A. J.

Viridans group streptococci (VS) from the oral cavity entering the bloodstream may initiate infective endocarditis (IE). We aimed to identify genes expressed in response to a pH increase from...

Altered Gene Expression in Staphylococcus aureus upon Interaction with Human Endothelial Cells

Vriesema, Aldwin J. M., Beekhuizen, Henry, Hamdi, Mohamed, Soufan, Alexandre, Lammers, Aart, Willekens, Ben, ...

Staphylococcus aureus is isolated from a substantial number of patients with infective endocarditis who are not known to have predisposing heart abnormalities. It has been suggested that the...

Multiple Mechanisms of Phase Variation of PorA in Neisseria meningitidis

Van Der Ende, Arie, Hopman, Carla T. P., Dankert, Jacob

Previously, we reported that PorA expression in Neisseria meningitidis is modulated by variation in the length of the homopolymeric tract of guanidine residues between the −35 and −10 regions of...

Interleukin-1 Receptor Type I Gene-Deficient Mice Are Less Susceptible to Staphylococcus epidermidis Biomaterial-Associated Infection than Are Wild-Type Mice

Boelens, Jaap Jan, Van Der Poll, Tom, Zaat, Sebastian A. J., Weening, Jan J., Dankert, Jacob

Elevated concentrations of interleukin-1 (IL-1) were found in tissue surrounding biomaterials infected with Staphylococcus epidermidis. To determine the role of IL-1 in biomaterial-associated...

NmeSI Restriction-Modification System Identified by Representational Difference Analysis of a Hypervirulent Neisseria meningitidis Strain

Bart, Aldert, Pannekoek, Yvonne, Dankert, Jacob, Van Der Ende, Arie

Neisseria meningitidis is a gram-negative bacterium that may cause meningitis, sepsis, or both. The increase in the incidence of meningococcal disease in various countries in the past 2 decades is...

Cloning of Genes of Nontypeable Haemophilus influenzae Involved in Penetration between Human Lung Epithelial Cells

Van Schilfgaarde, Muriel, Van Ulsen, Peter, Van Der Steeg, Wim, Winter, Victor, Eijk, Paul, Everts, Vincent, ...

Haemophilus influenzae penetrates between epithelial cells via an unknown mechanism. A chromosomal library of nonencapsulated H. influenzae strain A960053 DNA was constructed in Escherichia coli...

Characterization of Adherence of Nontypeable Haemophilus influenzae to Human Epithelial Cells

Van Schilfgaarde, Muriel, Van Ulsen, Peter, Eijk, Paul, Brand, Michiel, Stam, Martin, Kouame, Jamal, ...

The adherence of 58 nontypeable Haemophilus influenzae isolates obtained from patients with otitis media or chronic obstructive pulmonary disease (COPD) and obtained from the throats of healthy...

Normal IncA Expression and Fusogenicity of Inclusions in Chlamydia trachomatis Isolates with the incA I47T Mutation

Pannekoek, Yvonne, Van Der Ende, Arie, Eijk, Paul P., Van Marle, Jan, De Witte, Moniek A., Ossewaarde, Jacobus M., ...

To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were...

Mycoplasma pneumoniae P1 Type 1- and Type 2-Specific Sequences within the P1 Cytadhesin Gene of Individual Strains

Dorigo-Zetsma, J. Wendelien, Wilbrink, Berry, Dankert, Jacob, Zaat, Sebastian A. J.

Mycoplasma pneumoniae strains traditionally are divided into two types, based on sequence variation in the P1 gene. Recently, however, we have identified 8 P1 subtypes by restriction fragment length...

Molecular Typing of Borrelia burgdorferi Sensu Lato by Randomly Amplified Polymorphic DNA Fingerprinting Analysis

Wang, Guiqing, Van Dam, Alje P., Spanjaard, Lodewijk, Dankert, Jacob

To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from...

Randomly Amplified Polymorphic DNA Genotyping of Serogroup A Meningococci Yields Results Similar to Those Obtained by Multilocus Enzyme Electrophoresis and Reveals New Genotypes

Bart, Aldert, Schuurman, Ilse G. A., Achtman, Mark, Caugant, Dominique A., Dankert, Jacob, Van Der Ende, Arie

Randomly amplified polymorphic DNA (RAPD) genotyping was applied to one representative strain of each of the 84 electrophoretic types (ETs) of Neisseria meningitidis serogroup A previously defined by...

Antimicrobial Susceptibility of Haemophilus influenzae in the Respiratory Tracts of Patients with Cystic Fibrosis

Möller, Lieke V. M., Regelink, Annette G., Grasselier, Henny, Van Alphen, Loek, Dankert, Jacob

We analyzed the antimicrobial susceptibilities of Haemophilus influenzae isolates from 157 sputum specimens prospectively collected from 39 cystic fibrosis (CF) patients during a 2-year study. These...

cagA-Positive Helicobacter pylori Populations in China and The Netherlands Are Distinct

Van Der Ende, Arie, Pan, Zhi-Jun, Bart, Aldert, Feller, Monique, Xiao, Shu-Dong, ...

The aim of this research was to study whether and to what extent Chinese cagA-positive Helicobacter pylori isolates differ from those in The Netherlands. Analysis of random amplified polymorphic DNA...

Assessment of Clarithromycin-Resistant Helicobacter pylori among Patients in Shanghai and Guangzhou, China, by Primer-Mismatch PCR

Pan, Zhi-Jun, Su, Wan-Wen, Tytgat, Guido N. J., Dankert, Jacob, Van Der Ende, Arie

Of 96 Helicobacter pylori isolates from patients in Shanghai and Guangzhou, China, 5 had the A2143G 23S rRNA mutation as determined by primer-mismatch PCR and were resistant to clarithromycin by the...

Sensitivity of Three Urinary Antigen Tests Associated with Clinical Severity in a Large Outbreak of Legionnaires' Disease in The Netherlands

Yzerman, Ed P. F., Boer, Jeroen W. Den, Lettinga, Kamilla D., Schellekens, Joop, Dankert, Jacob, Peeters, Marcel

In 1999 an outbreak involving 188 patients with Legionnaires' disease (LD) occurred among visitors to a flower show in the Netherlands. Two enzyme immunoassays (Binax and Biotest) and one...

Detection of Imported Malaria with the Cell-Dyn 4000 Hematology Analyzer

Wever, Peter C., Henskens, Yvonne M. C., Kager, Piet A., Dankert, Jacob, Van Gool, Tom

The sensitivity and specificity of the Cell-Dyn 4000 hematology analyzer in the diagnosis of imported malaria were studied with samples from patients in an academic hospital setting. The performance...

Detection of a Point Mutation Associated with High-Level Isoniazid Resistance in Mycobacterium tuberculosis by Using Real-Time PCR Technology with 3′-Minor Groove Binder-DNA Probes

Van Doorn, H. Rogier, Claas, Eric C. J., Templeton, Kate E., Te Koppele Vije, Arianne, De Jong, Menno D., ...

Tuberculosis remains one of the leading infectious causes of death worldwide. The emergence of drug-resistant strains of Mycobacterium tuberculosis is a serious public health threat. Resistance to...

Major Histocompatibility Complex Class I Peptide Presentation after Salmonella enterica Serovar Typhimurium Infection Assessed via Stable Isotope Tagging of the B27-Presented Peptide Repertoire

Ringrose, Jeffrey H., Meiring, Hugo D., Speijer, Dave, Feltkamp, Theodorus E. W., ...

Reactive arthritis (ReA) induced by infection with several gram-negative bacteria is strongly associated with expression of the major histocompatibility complex class I molecule HLA-B27. It is...

Interleukin 1α Increases the Susceptibility of Rabbits to Experimental Viridans Streptococcal Endocarditis

Dankert, Jacob, Van Der Werff, Janneke, Joldersma, Willem, Zaat, Sebastian A. J.

Major predisposing conditions for infective endocarditis (IE) are the presence of a cardiac platelet-fibrin vegetation and of circulating bacteria with relatively low susceptibility to microbicidal...

Peri-Implant Tissue Is an Important Niche for Staphylococcus epidermidis in Experimental Biomaterial-Associated Infection in Mice▿

Broekhuizen, Corine A. N., De Boer, Leonie, Schipper, Kim, Jones, Christopher D., Quadir, Shan, Feldman, Roger G., ...

Biomaterial-associated infections (BAI), which are predominantly caused by Staphylococcus epidermidis, are a significant problem in modern medicine. Biofilm formation is considered the pivotal...