Accelerating phage-display library selection by reversible and site-specific biotinylation (2009)
Koide, Akiko, Wojcik, John, Gilbreth, Ryan N., Reichel, Annett, Piehler, Jacob, Koide, Shohei
Immobilization of a target molecule to a solid support is an indispensable step in phage display library sorting. Here we describe an immobilization method that addresses shortcomings of existing...
Cell-free production of G protein-coupled receptors for functional and structural studies (2007)
Klammt, Christian, Schwarz, Daniel, Eifler, Nora, Engel, Andreas, Piehler, Jacob, Haase, Winfried, ...
Martynas Gavutis, Suman Lata, Peter Lamken, Pia Müller, Jacob Piehler
ABSTRACT We describe an experimental approach for studying ligand-receptor interactions in the plane of the membrane. The extracellular domains of the type I interferon receptor subunits ifnar1-EC...
Zugl.: Tübingen, Universiẗat, Diss., 1997.
Roisman, Laila C., Piehler, Jacob, Trosset, Jean-Yves, Scheraga, Harold A., Schreiber, Gideon
The pleiotropic activity of type I interferons has been attributed to the specific interaction of IFN with the cell-surface receptor components ifnar1 and ifnar2. To date, the structure of IFN has...
Jaitin, Diego A., Roisman, Laila C., Jaks, Eva, Gavutis, Martynas, Piehler, Jacob, Van Der Heyden, Jose, ...
Alpha and beta interferons (IFN-α and IFN-β) are multifunctional cytokines that exhibit differential activities through a common receptor composed of the subunits IFNAR1 and IFNAR2. Here we...
Gavutis, Martynas, Lata, Suman, Lamken, Peter, Müller, Pia, Piehler, Jacob
We describe an experimental approach for studying ligand-receptor interactions in the plane of the membrane. The extracellular domains of the type I interferon receptor subunits ifnar1-EC and...
Roisman, Laila C., Piehler, Jacob, Trosset, Jean-Yves, Scheraga, Harold A., Schreiber, Gideon
The pleiotropic activity of type I interferons has been attributed to the specific interaction of IFN with the cell-surface receptor components ifnar1 and ifnar2. To date, the structure of IFN has...
Gavutis, Martynas, Lata, Suman, Lamken, Peter, Müller, Pia, Piehler, Jacob
We describe an experimental approach for studying ligand-receptor interactions in the plane of the membrane. The extracellular domains of the type I interferon receptor subunits ifnar1-EC and...
Jaitin, Diego A., Roisman, Laila C., Jaks, Eva, Gavutis, Martynas, Piehler, Jacob, Van Der Heyden, Jose, ...
Alpha and beta interferons (IFN-α and IFN-β) are multifunctional cytokines that exhibit differential activities through a common receptor composed of the subunits IFNAR1 and IFNAR2. Here we...
Determination of the Two-Dimensional Interaction Rate Constants of a Cytokine Receptor Complex
Gavutis, Martynas, Jaks, Eva, Lamken, Peter, Piehler, Jacob
Ligand-receptor interactions within the plane of the plasma membrane play a pivotal role for transmembrane signaling. The biophysical principles of protein-protein interactions on lipid bilayers,...
Processive kinesins require loose mechanical coupling for efficient collective motility
Bieling, Peter, Telley, Ivo A, Piehler, Jacob, Surrey, Thomas
Processive motor proteins are stochastic steppers that perform actual mechanical steps for only a minor fraction of the time they are bound to the filament track. Motors usually work in teams and...