Jacqueline Noaillac-Depeyre

The Role of the Schizosaccharomyces pombe gar2 Protein in Nucleolar Structure and Function Depends on the Concerted Action of its Highly Charged N Terminus and its RNA-binding Domains

Sicard, Hélène, Faubladier, Marlène, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Gas, Nicole, Caizergues-Ferrer, Michèle

Nonribosomal nucleolar protein gar2 is required for 18S rRNA and 40S ribosomal subunit production in Schizosaccharomyces pombe. We have investigated the consequences of the absence of each structural...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we show that...

Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs†

Dez, Christophe, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Henry, Yves

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. The ways in which these particles are assembled and correctly localized...

Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein

Vanrobays, Emmanuel, Gleizes, Pierre-Emmanuel, Bousquet-Antonelli, Cécile, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Gélugne, Jean-Paul

Numerous non-ribosomal trans-acting factors involved in pre-ribosomal RNA processing have been characterized, but none of them is specifically required for the last cytoplasmic steps of 18S rRNA...

Sequential Protein Association with Nascent 60S Ribosomal Particles

Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...

Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...

Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification†

Dez, Christophe, Froment, Carine, Noaillac-Depeyre, Jacqueline, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, Henry, Yves

We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S...

The Role of the Schizosaccharomyces pombe gar2 Protein in Nucleolar Structure and Function Depends on the Concerted Action of its Highly Charged N Terminus and its RNA-binding Domains

Sicard, Hélène, Faubladier, Marlène, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Gas, Nicole, Caizergues-Ferrer, Michèle

Nonribosomal nucleolar protein gar2 is required for 18S rRNA and 40S ribosomal subunit production in Schizosaccharomyces pombe. We have investigated the consequences of the absence of each structural...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we show that...

Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs†

Dez, Christophe, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Henry, Yves

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. The ways in which these particles are assembled and correctly localized...

Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein

Vanrobays, Emmanuel, Gleizes, Pierre-Emmanuel, Bousquet-Antonelli, Cécile, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Gélugne, Jean-Paul

Numerous non-ribosomal trans-acting factors involved in pre-ribosomal RNA processing have been characterized, but none of them is specifically required for the last cytoplasmic steps of 18S rRNA...

Sequential Protein Association with Nascent 60S Ribosomal Particles

Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...

Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...

Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification†

Dez, Christophe, Froment, Carine, Noaillac-Depeyre, Jacqueline, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, Henry, Yves

We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S...

Impaired ribosome biogenesis in Diamond-Blackfan anemia

Choesmel, Valérie, Bacqueville, Daniel, Rouquette, Jacques, Noaillac-Depeyre, Jacqueline, Fribourg, Sébastien, Crétien, Aurore, ...

The gene encoding the ribosomal protein S19 (RPS19) is frequently mutated in Diamond-Blackfan anemia (DBA), a congenital erythroblastopenia. The consequence of these mutations on the onset of the...

Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex

Gleizes, Pierre-Emmanuel, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Teulières, Frédéric, Dauxois, Jean-Yves, Pommet, Denys, ...

To study the nuclear export of preribosomes, ribosomal RNAs were detected by in situ hybridization using fluorescence and EM, in the yeast Saccharomyces cerevisiae. In wild-type cells,...