James A. Fraser

Evolution of the Mating Type Locus: Insights Gained from the Dimorphic Primary Fungal Pathogens Histoplasma capsulatum, (2007)

Coccidioides Immitis, Coccidioides Posadasii, James A. Fraser, Jason E. Stajich, Eric J. Tarcha, Garry T. Cole, ...

Sexual reproduction of fungi is governed by the mating type (MAT) locus, a specialized region of the genome encoding key transcriptional regulators that direct regulatory networks to specify cell...

Recent Evolution of the Human Pathogen Cryptococcus neoformans by Intervarietal Transfer of a 14-Gene Fragment (2006)

Kavanaugh, Laura A., Fraser, James A., Dietrich, Fred S.

The availability of the whole-genome sequence from the 2 known varieties of the human pathogenic fungus Cryptococcus neoformans provides an opportunity to study the relative contribution of...

Recent Evolution of the Human Pathogen Cryptococcus neoformans by Inter-Varietal Transfer of a 14 Gene Fragment (2006)

Kavanaugh, Laura A., Fraser, James A., Dietrich, Fred S.

The availability of the whole-genome sequence from the two known varieties of the human pathogenic fungus Cryptococcus neoformans provides an opportunity to study the relative contribution of...

Convergent Evolution of Chromosomal Sex-Determining Regions in the Animal and Fungal Kingdoms (2004)

James A. Fraser, Stephanie Diezmann, Ryan L. Subaran, Andria Allen, Klaus B. Lengeler, Fred S. Dietrich, ...

A comparative genomic analysis of the sex determining region in fungi reveals a remarkable similarity between its evolution and the events which shaped mammalian sex chromosomes

Convergent Evolution of Chromosomal Sex-Determining Regions in the Animal and Fungal Kingdoms (2004)

James A. Fraser, Stephanie Diezmann, Ryan L. Subaran, Andria Allen, Klaus B. Lengeler, Fred S. Dietrich, ...

Sexual identity is governed by sex chromosomes in plants and animals, and by mating type (MAT) loci in fungi. Comparative analysis of the MAT locus from a species cluster of the human fungal pathogen...

Convergent evolution of chromosomal sex-determining regions in the animal and fungal kingdoms (2004)

Fraser, James A., Diezmann, Stephanie, Subaran, Ryan L., Allen, Andria, Lengeler, Klaus B., Dietrich, Fred S., ...

Sexual identity is governed by sex chromosomes in plants and animals, and by mating type (MAT) loci in fungi. Comparative analysis of the MAT locus from a species cluster of the human fungal pathogen...

Convergent evolution of chromosomal sex-determining regions in the animal and fungal kingdoms (2004)

Fraser, James A., Diezmann, Stephanie, Subaran, Ryan L., Allen, Andria, Lengeler, Klaus B., Dietrich, Fred S., ...

Sexual identity is governed by sex chromosomes in plants and animals, and by mating type (MAT) loci in fungi. Comparative analysis of the MAT locus from a species cluster of the human fungal pathogen...

Isolation and Characterization of Two Ammonium Permease Genes, meaA and mepA, from Aspergillus nidulans

Monahan, Brendon J., Fraser, James A., Hynes, Michael J., Davis, Meryl A.

Ammonium and the analogue methylammonium are taken into the cell by active transport systems which constitute a family of transmembrane proteins that have been identified in fungi, bacteria, plants,...

A Gene from Aspergillus nidulans with Similarity to URE2 of Saccharomyces cerevisiae Encodes a Glutathione S-Transferase Which Contributes to Heavy Metal and Xenobiotic Resistance

Fraser, James A., Davis, Meryl A., Hynes, Michael J.

Aspergillus nidulans is a saprophytic ascomycete that utilizes a wide variety of nitrogen sources. We identified a sequence from A. nidulans similar to the glutathione S-transferase-like nitrogen...

Mating-Type Locus of Cryptococcus neoformans: a Step in the Evolution of Sex Chromosomes

Lengeler, Klaus B., Fox, Deborah S., Fraser, James A., Allen, Andria, Forrester, Keri, Dietrich, Fred S., ...

The sexual development and virulence of the fungal pathogen Cryptococcus neoformans is controlled by a bipolar mating system determined by a single locus that exists in two alleles, α and a. The α...

Recapitulation of the Sexual Cycle of the Primary Fungal Pathogen Cryptococcus neoformans var. gattii: Implications for an Outbreak on Vancouver Island, Canada†

Fraser, James A., Subaran, Ryan L., Nichols, Connie B., Heitman, Joseph

Cryptococcus neoformans is a human fungal pathogen that exists as three distinct varieties or sibling species: the predominantly opportunistic pathogens C. neoformans var. neoformans (serotype D) and...

PAK Kinases Ste20 and Pak1 Govern Cell Polarity at Different Stages of Mating in Cryptococcus neoformans

Nichols, Connie B., Fraser, James A., Heitman, Joseph

Sexual identity and mating are linked to virulence of the fungal pathogen Cryptococcus neoformans. Cells of the α mating type are more prevalent and can be more virulent than a cells, and...

Convergent Evolution of Chromosomal Sex-Determining Regions in the Animal and Fungal Kingdoms

Fraser, James A, Diezmann, Stephanie, Subaran, Ryan L, Allen, Andria, Lengeler, Klaus B, Dietrich, Fred S, ...

Sexual identity is governed by sex chromosomes in plants and animals, and by mating type (MAT) loci in fungi. Comparative analysis of the MAT locus from a species cluster of the human fungal pathogen...

Chromosomal Translocation and Segmental Duplication in Cryptococcus neoformans†

Fraser, James A., Huang, Johnny C., Pukkila-Worley, Read, Alspaugh, J. Andrew, Mitchell, Thomas G., Heitman, Joseph

Large chromosomal events such as translocations and segmental duplications enable rapid adaptation to new environments. Here we marshal genomic, genetic, meiotic mapping, and physical evidence to...

Clinical and Environmental Isolates of Cryptococcus gattii from Australia That Retain Sexual Fecundity†

Campbell, Leona T., Fraser, James A., Nichols, Connie B., Dietrich, Fred S., Carter, Dee, Heitman, Joseph

Cryptococcus gattii is a primary pathogenic yeast that causes disease in both animals and humans. It is closely related to Cryptococcus neoformans and diverged from a common ancestor ∼40 million...

Nuclear Accumulation of the GATA Factor AreA in Response to Complete Nitrogen Starvation by Regulation of Nuclear Export

Todd, Richard B., Fraser, James A., Wong, Koon Ho, Davis, Meryl A., Hynes, Michael J.

Both the availability and the quality of nutrients affect cellular functions by controlling gene activity. AreA, a member of the GATA family of transcription factors, globally activates expression of...

Slow volume transients in amphibian skeletal muscle fibres studied in hypotonic solutions

Fraser, James A, Rang, Catherine EJ, Usher-Smith, Juliet A, Huang, Christopher L-H

The influence of extracellular hypotonicity on the relationship between cell volume (Vc) and resting membrane potential (Em) was investigated in Rana temporaria skeletal muscle. Vc was measured by...

A quantitative analysis of cell volume and resting potential determination and regulation in excitable cells

Fraser, James A, Huang, Christopher L-H

This paper quantifies recent experimental results through a general physical description of the mechanisms that might control two fundamental cellular parameters, resting potential (Em) and cell...

The effect of intracellular acidification on the relationship between cell volume and membrane potential in amphibian skeletal muscle

Fraser, James A, Middlebrook, Claire E, Usher-Smith, Juliet A, Schwiening, Christof J, Huang, Christopher L-H

The relationship between cell volume (Vc) and membrane potential (Em) in Rana temporaria striated muscle fibres was investigated under different conditions of intracellular acidification. Confocal...

Membrane potential stabilization in amphibian skeletal muscle fibres in hypertonic solutions

Ferenczi, Emily A, Fraser, James A, Chawla, Sangeeta, Skepper, Jeremy N, Schwiening, Christof J, Huang, Christopher L-H

This study investigated membrane transport mechanisms influencing relative changes in cell volume (V) and resting membrane potential (Em) following osmotic challenge in amphibian skeletal muscle...

Isolation and Characterization of Two Ammonium Permease Genes, meaA and mepA, from Aspergillus nidulans

Monahan, Brendon J., Fraser, James A., Hynes, Michael J., Davis, Meryl A.

Ammonium and the analogue methylammonium are taken into the cell by active transport systems which constitute a family of transmembrane proteins that have been identified in fungi, bacteria, plants,...

A Gene from Aspergillus nidulans with Similarity to URE2 of Saccharomyces cerevisiae Encodes a Glutathione S-Transferase Which Contributes to Heavy Metal and Xenobiotic Resistance

Fraser, James A., Davis, Meryl A., Hynes, Michael J.

Aspergillus nidulans is a saprophytic ascomycete that utilizes a wide variety of nitrogen sources. We identified a sequence from A. nidulans similar to the glutathione S-transferase-like nitrogen...

Mating-Type Locus of Cryptococcus neoformans: a Step in the Evolution of Sex Chromosomes

Lengeler, Klaus B., Fox, Deborah S., Fraser, James A., Allen, Andria, Forrester, Keri, Dietrich, Fred S., ...

The sexual development and virulence of the fungal pathogen Cryptococcus neoformans is controlled by a bipolar mating system determined by a single locus that exists in two alleles, α and a. The α...

Recapitulation of the Sexual Cycle of the Primary Fungal Pathogen Cryptococcus neoformans var. gattii: Implications for an Outbreak on Vancouver Island, Canada†

Fraser, James A., Subaran, Ryan L., Nichols, Connie B., Heitman, Joseph

Cryptococcus neoformans is a human fungal pathogen that exists as three distinct varieties or sibling species: the predominantly opportunistic pathogens C. neoformans var. neoformans (serotype D) and...

PAK Kinases Ste20 and Pak1 Govern Cell Polarity at Different Stages of Mating in Cryptococcus neoformans

Nichols, Connie B., Fraser, James A., Heitman, Joseph

Sexual identity and mating are linked to virulence of the fungal pathogen Cryptococcus neoformans. Cells of the α mating type are more prevalent and can be more virulent than a cells, and...

Convergent Evolution of Chromosomal Sex-Determining Regions in the Animal and Fungal Kingdoms

Fraser, James A, Diezmann, Stephanie, Subaran, Ryan L, Allen, Andria, Lengeler, Klaus B, Dietrich, Fred S, ...

Sexual identity is governed by sex chromosomes in plants and animals, and by mating type (MAT) loci in fungi. Comparative analysis of the MAT locus from a species cluster of the human fungal pathogen...

Chromosomal Translocation and Segmental Duplication in Cryptococcus neoformans†

Fraser, James A., Huang, Johnny C., Pukkila-Worley, Read, Alspaugh, J. Andrew, Mitchell, Thomas G., Heitman, Joseph

Large chromosomal events such as translocations and segmental duplications enable rapid adaptation to new environments. Here we marshal genomic, genetic, meiotic mapping, and physical evidence to...

Clinical and Environmental Isolates of Cryptococcus gattii from Australia That Retain Sexual Fecundity†

Campbell, Leona T., Fraser, James A., Nichols, Connie B., Dietrich, Fred S., Carter, Dee, Heitman, Joseph

Cryptococcus gattii is a primary pathogenic yeast that causes disease in both animals and humans. It is closely related to Cryptococcus neoformans and diverged from a common ancestor ∼40 million...

Nuclear Accumulation of the GATA Factor AreA in Response to Complete Nitrogen Starvation by Regulation of Nuclear Export

Todd, Richard B., Fraser, James A., Wong, Koon Ho, Davis, Meryl A., Hynes, Michael J.

Both the availability and the quality of nutrients affect cellular functions by controlling gene activity. AreA, a member of the GATA family of transcription factors, globally activates expression of...

Slow volume transients in amphibian skeletal muscle fibres studied in hypotonic solutions

Fraser, James A, Rang, Catherine EJ, Usher-Smith, Juliet A, Huang, Christopher L-H

The influence of extracellular hypotonicity on the relationship between cell volume (Vc) and resting membrane potential (Em) was investigated in Rana temporaria skeletal muscle. Vc was measured by...

Membrane potential stabilization in amphibian skeletal muscle fibres in hypertonic solutions

Ferenczi, Emily A, Fraser, James A, Chawla, Sangeeta, Skepper, Jeremy N, Schwiening, Christof J, Huang, Christopher L-H

This study investigated membrane transport mechanisms influencing relative changes in cell volume (V) and resting membrane potential (Em) following osmotic challenge in amphibian skeletal muscle...

A quantitative analysis of cell volume and resting potential determination and regulation in excitable cells

Fraser, James A, Huang, Christopher L-H

This paper quantifies recent experimental results through a general physical description of the mechanisms that might control two fundamental cellular parameters, resting potential (Em) and cell...

The effect of intracellular acidification on the relationship between cell volume and membrane potential in amphibian skeletal muscle

Fraser, James A, Middlebrook, Claire E, Usher-Smith, Juliet A, Schwiening, Christof J, Huang, Christopher L-H

The relationship between cell volume (Vc) and membrane potential (Em) in Rana temporaria striated muscle fibres was investigated under different conditions of intracellular acidification. Confocal...

The contribution of refractoriness to arrhythmic substrate in hypokalemic Langendorff-perfused murine hearts

Sabir, Ian N., Fraser, James A., Killeen, Matthew J., Grace, Andrew A., Huang, Christopher L.-H.

The clinical effects of hypokalemia including action potential prolongation and arrhythmogenicity suppressible by lidocaine were reproduced in hypokalemic (3.0 mM K+) Langendorff-perfused murine...

The influence of intracellular lactate and H+ on cell volume in amphibian skeletal muscle

Usher-Smith, Juliet A, Fraser, James A, Bailey, Peter S J, Griffin, Julian L, Huang, Christopher L-H

The combined effects of intracellular lactate and proton accumulation on cell volume, Vc, were investigated in resting Rana temporaria striated muscle fibres. Intracellular lactate and H+...

Evolution of the Mating Type Locus: Insights Gained from the Dimorphic Primary Fungal Pathogens Histoplasma capsulatum, Coccidioides immitis, and Coccidioides posadasii▿ †

Fraser, James A., Stajich, Jason E., Tarcha, Eric J., Cole, Garry T., Inglis, Diane O., Sil, Anita, ...

Sexual reproduction of fungi is governed by the mating type (MAT) locus, a specialized region of the genome encoding key transcriptional regulators that direct regulatory networks to specify cell...

First Contemporary Case of Human Infection with Cryptococcus gattii in Puget Sound: Evidence for Spread of the Vancouver Island Outbreak▿

Upton, Arlo, Fraser, James A., Kidd, Sarah E., Bretz, Camille, Bartlett, Karen H., Heitman, Joseph, ...

We report a case of cryptococcosis due to C. gattii which appears to have been acquired in the Puget Sound region, Washington State. Genotyping confirmed identity to the predominant Vancouver Island...

A quantitative analysis of the effect of cycle length on arrhythmogenicity in hypokalaemic Langendorff-perfused murine hearts

Sabir, Ian N., Fraser, James A., Cass, Thomas R., Grace, Andrew A., Huang, Christopher L.-H.

The clinically established proarrhythmic effect of bradycardia and antiarrhythmic effect of lidocaine (10 μM) were reproduced in hypokalaemic (3.0 mM K+) Langendorff-perfused murine hearts paced...

Transitions in Sexuality: Recapitulation of an Ancestral Tri- and Tetrapolar Mating System in Cryptococcus neoformans▿ †

Hsueh, Yen-Ping, Fraser, James A., Heitman, Joseph

Sex is orchestrated by the mating-type locus (MAT) in fungi and by sex chromosomes in plants and animals. In fungi, two patterns of sexuality occur: bipolar with a single, typically biallelic sex...

Extracellular Charge Adsorption Influences Intracellular Electrochemical Homeostasis in Amphibian Skeletal Muscle

Mehta, Arpan R., Huang, Christopher L.-H., Skepper, Jeremy N., Fraser, James A.

The membrane potential measured by intracellular electrodes, Em, is the sum of the transmembrane potential difference (E1) between inner and outer cell membrane surfaces and a smaller potential...