Characterization of a monothiol glutaredoxin encoded by Chlorella virus PBCV-1 (2009)
Fitzgerald, Lisa A, Zhang, Yuanzheng, Lewis, Gentry, Van Etten, James L.
Annotation of the 330-kb Chlorella virus PBCV-1 genome identified a 237 nucleotide gene (a438l) that codes for a protein with ~35% amino acid identity to glutaredoxins (Grx) found in other organisms....
Agarkova, Irina V., Dunigan, David, Gurnon, James R., Greiner, Timo, Barres, Julia, Thiel, Gerhard, ...
Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family of large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from the...
Putative Gene Promoter Sequences in the Chlorella Viruses (2008)
Fitzgerald, Lisa, Boucher, Philip, Yanai-Balser, Giane, Suhre, Karsten, Graves, Michael, Van Etten, James L.
Three short (7 to 9 nucleotides) highly conserved nucleotide sequences were identified in the putative promoter regions (150 bp upstream and 50 bp downstream of the ATG translation start site) of...
Neupartl, Monika, Meyer, Christine, Woll, Isabell, Frohns, Florian, Kang, Ming, Van Etten, James L., ...
Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of...
Functional Implication of the tRNA Genes Encoded in the Chlorella Virus PBCV-l Genome (2008)
Lee, Da Young, Graves, Michael V., Van Etten, James L., Choi, Tae-Jin
The prototype Chlorella virus PBCV-l encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative...
Myron Kendall Brakke: 1921 to 2007 (2008)
Scholthof, Karen-Beth G, Jackson, Andrew O, Van Etten, James L
Plant pathology lost a distinguished pioneer in plant virology with the death of Dr. Myron K. Brakke on 15 June 2007. Those who were his close colleagues also lost a valued mentor, an irreplaceable...
Zhang, Yuanzheng, Maley, Frank, Maley, Gladys F., Duncan, Garry, Dunigan, David, Van Etten, James L.
The chlorovirus PBCV-1, like many large double-stranded DNA-containing viruses, contains several genes that encode putative proteins involved in nucleotide biosynthesis. This report describes the...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Hartigan, James, Van Etten, James L.
Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Hartigan, James, Van Etten, James L.
Appendix A: Gene Names m002R through m843L Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, %...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Hartigan, James, Van Etten, James L.
Appendix B: Gene Names M001L through M807R Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, %...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Hartigan, James, Van Etten, James L.
Appendix C: Gene Names n001L through n849RDocument, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, %...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Hartigan, James, Van Etten, James L.
Appendix D: Gene Names N003L through N847R Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, %...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Nierman, Willaim C., Van Etten, James L.
Viruses NY-2A and AR158, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Nierman, William C., Van Etten, James L.
Appendix A: Gene Names b002R - b797RDocument shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Nierman, William C., Van Etten, James L.
Appendix B: Gene Names B001L - B886R Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Nierman, William C., Van Etten, James L.
Appendix C: Gene Names c001R - c814L Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query...
Fitzgerald, Lisa A., Graves, Michael V., Li, Xiao, Feldblyum, Tamara, Nierman, Willaim C., Van Etten, James L.
Appendix D: Gene Names C006R - C815L Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query...
Chan, Siu-Hong, Zhu, Zhenyu, Dunigan, David, Van Etten, James L., Xu, Shuang-yong
Chlorella virus NY-2A has a large, highly methylated dsDNA genome (45% of the cytosines are 5-methylcytosine and 37% of the adenines are N6-methyladenine). Here, we report the cloning, expression,...
Virion-Associated Restriction Endonucleases of Chloroviruses (2006)
Agarkova, Irina V., Dunigan, David, Van Etten, James L.
Chloroviruses are large, double-stranded-DNA, plaque-forming viruses that infect certain eukaryotic chlorella- like green algae. The prototype of the genus is Paramecium bursaria chlorella virus 1...
Phycodnaviruses: A peek at genetic diversity (2006)
Dunigan, David, Fitzgerald, Lisa, Van Etten, James L.
The family Phycodnaviridae encompasses a diverse collection of large icosahedral, dsDNA viruses infecting algae. These viruses have genomes ranging from 160 to 560 kb. The family consists of six...
Frohns, Florian, Kasmann, Anja, Kramer, Detlef, Schafer, Britta, Mehmel, Mario, Kang, Ming, ...
revious studies have established that chlorella viruses encode K+ channels with different structural and functional properties. In the current study, we exploit the different sensitivities of these...
Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima (2005)
Zhang, Yuanzheng, Moriyama, Hideaki, Homma, Kohei, Van Etten, James L.
A putative deoxyuridine triphosphatase (dUTPase) gene from chlorella virus PBCV-1 was cloned, and the recombinant protein was expressed in Escherichia coli. The recombinant protein has dUTPase...
Chlorovirus: A Genus of Phycodnaviridae that Infects Certain Chlorella-Like Green Algae (2005)
Kang, Ming, Dunigan, David, Van Etten, James L.
Taxonomy: Chlorella viruses are assigned to the family Phycodnaviridae, genus Chlorovirus, and are divided into three species: Chlorella NC64A viruses, Chlorella Pbi viruses and Hydra viridis...
Chan, Siu-hong, Zhu, Zhenyu, Van Etten, James L., Xu, Shuang-yong
The cloning and expression of the CviPII DNA nicking and modification system encoded by chlorella virus NYs-1 is described. The system consists of a co-linear MTase encoding gene (cviPIIM) and a...
Glycosyltransferases encoded by viruses. (2004)
Markine-Goriaynoff, Nicolas, Gillet, Laurent, Van Etten, James L, Korres, Haralambos, Verma, Naresh, Vanderplasschen, Alain
Studies of cellular biology in recent decades have highlighted the crucial roles of glycans in numerous important biological processes, raising the concept of glycomics that is now considered as...
Roberts,Richard J., Belfort,Marlene, Bestor,Timothy, Bhagwat,Ashok S., Bickle,Thomas A., Bitinaite,Jurate, ...
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different...
Roberts, Richard J, Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S, Bickle, Thomas A, Bitinaite, Jurate, ...
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different...
Roberts, Richard J., Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S., Bickle, Thomas A., Bitinaite, Jurate, ...
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different...
Roberts, Richard J, Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S, Bickle, Thomas A, Bitinaite, Jurate, ...
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different...
Roberts, Richard J., Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S., Bickle, Thomas A., Bitinaite, Jurate, ...
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different...
Nandhagopal, Narayanasamy, Simpson, Alan, Gurnon, James, Yan, Xiadong, Baker, Timothy, Graves, Michael V., ...
Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a glycoprotein coat consisting of pseudohexagonal arrays of...
Ornithine Decarboxylase Encoded by Chlorella Virus PBCV-1 (2002)
Gurnon, James R., Adams, Byron, Nickerson, Kenneth, Fitzgerald, Lisa A., Van Etten, James L.
Sequence analysis of the 330-kb genome of chlorella virus PBCV-1 revealed an open reading frame, A207R, which encodes a protein with 37-41% amino acid identity to ornithine decarboxylase (ODC) from...
Isolation and characterization of a virus that infects Emiliania huxleyi (Haptophyta) (2002)
Castberg, Tonje, Thyrhaug, Runar, Larsen, Aud, Sandaa, Ruth-Anne, Heldal, Mikal, Van Etten, James L., ...
The isolation and characterization of a virus (designated EhV) that infects the marine coccolithophorid Emiliania huxleyi (Lohmann) Hay & Mohler are described. Three independent clones of EhV...
A Potassium Channel Protein Encoded by Chlorella Virus PBCV-1 (2000)
Plugge, B., Gazzarrini, S., Nelson, M., Cerana, R., Van Etten, James L., Derst, C., ...
The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), en¬codes a 94-codon open reading frame (ORF) that contains a motif resembling the signature se¬quence of the pore...
Giant Viruses Infecting Algae (1999)
Van Etten, James L., Meints, Russel
Paramecium bursaria chlorella virus (PBCV-1) is the prototype of a family of large, icosahedral, plaque-forming, double-stranded-DNA-containing viruses that replicate in certain unicellular,...
Hyaluronan Synthase of Chlorella Virus PBCV-1 (1997)
DeAngelis, Paul L., Jing, Wei, Graves, Michael V., Burbank, Dwight E., Van Etten, James L.
Sequence analysis of the 330-kilobase genome of the virus PBCV-1 that infects a chlorella-like green algae revealed an open reading frame, A98R, with similarity to several hyaluronan synthases....
Chlorella Virus PBCV-1 Encodes a Homolog of the Bacteriophage T4 UV Damage Repair Gene denV (1997)
Furuta, Masakazu, Schrader, John, Schrader, Holly, Kokjohn, Tyler, Nyaga, Simon, McCullough, Amanda, ...
The bacteriophage T4 denV gene encodes a well-characterized DNA repair enzyme involved in pyrimidine photodimer excision. We have discovered the first homologs of the denV gene in chlorella viruses,...
Characterization of an ATP-Dependent DNA Ligase Encoded by Chlorella Virus PBCV-1 (1997)
Ho, C. Kiong, Van Etten, James L., Shuman, Stewart
We report that Chlorella virus PBCV-1 encodes a 298-amino-acid ATP-dependent DNA ligase. The PBCV-1 enzyme is the smallest member of the covalent nucleotidyl transferase superfamily, which includes...
Expression and Characterization of an RNA Capping Enzyme Encoded by Chlorella Virus PBCV-1 (1996)
Ho, C. Kiong, Van Etten, James L., Shuman, Stewart
We report that the A103R protein of Chlorella virus PBCV-1 is an mRNA capping enzyme that catalyzes the transfer of GMP from GTP to the 5' diphosphate end of RNA. This is a two-step reaction in which...
Restriction generated oligonucleotides utilizing the two base recognition endonuclease CwJI (1994)
Swaminathan, Neela, George, David, McMaster, Karolyn, Szablewski, Jim, Van Etten, James L., Mead, David A.
The conversion of an anonymous DNA sample into numerous oligonucleotides is enzymatlcally feasible using an unusual restriction endonuclease, CViJI. Depending on reaction conditions, CWJI is capable...
Leptospira Genomes Are Modified at 5'-GTAC (1993)
Ralph, David, Que, Quideng, Van Etten, James L., McClelland, Michael
Genomic DNAs of 14 strains from seven species of the spirochete Leptospira were resistant to cleavage by the restriction endonuclease RsaI 1 (5'-GTAC). A modified base comigrating with m4C was...
Evidence for Virus-Encoded Glycosylation Specificity (1993)
Wang, Ing-Nang, Li, Yu, Que, Quideng, Bhattacharya, Meenakshi, Lane, Leslie C., Chaney, William G., ...
Four spontaneously derived serologically distinct classes of mutants of the Paramecium bursaria chlorella virus (PBCV-1) were isolated using polydonal antiserum prepared against either intact PBCV-1...
Rapid shotgeun cloning utillizing the two base recongition endonuclease CviJI (1992)
Fitzgerald, Michael C., Skowron, Peter, Van Etten, James L., Smith, Lloyd M., Maed, David A.
A new approach has been developed for the rapid fragmentation and fractionation of DNA Into a size suitable for shotgun cloning and sequencing. The restriction endonuclease CviJI normally cleaves the...
Zhang, Yanping, Nelson, Michael, Van Etten, James L.
The chlorelia virus PBCV−1 contains an open reading frame, named P17−ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia...
Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system (1992)
Zhang, Yanping, Nelson, Michael, Nietfeldt, Joseph W., Burbank, Dwight E., Van Etten, James L.
A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells....
DNA sequencing of four bases using three lanes (1992)
Nelson, Michael, Van Etten, James L., Grabherr, Reingard
A three-lane DNA sequencing strategy Is described which is based on redundant binary coding principles from communications theory. Three-lane sequencing Is an efficient, accurate, and flexible...
Viruses and Virus-like Particles of Eukaryotic Algae (1991)
Van Etten, James L., Lane, Leslie C., Meints, Russel H.
TABLE OF CONTENTS: INTRODUCTION CHARACTERISTICS OF CHLORELLA SPECIES General Properties of Chlorella Species Symbiotic Chlorella Species Chlorella Strains NC64A, Nla, Pbi, and SAG-241-80DISCOVERY OF...
A Phylogenetic Analysis of the Mycoplasmas: Basis for Their Classification (1989)
Weisburg, W. G., Tully, J. G., Rose, D. L., Petzel, J. P., Oyaizu, H., Yang, D., ...
Small-subunit rRNA sequences were determined for almost 50 species of mycoplasmas and their walled relatives, providing the basis for a phylogenetic systematic analysis of these organisms. Five...
ROHOZINSKI, JAN, VAN ETTEN, JAMES L.
DNA polymerases were purified several hundred-fold from the 10 000 × g soluble (polymerase I) and particulate (polymerase III) fractions prepared from virus PBCV-1 infected Chlorella NC64A extracts....
Chlorella Viruses Isolated in China (1988)
Zhang, Yanping, Burbank, Dwight, Van Etten, James L.
Plaque-forming viruses of the unicellular, eucaryotic, exsymbiotic, Chlorella-like green algae strain NC64A, which are common in the United States, were also present in fresh water collected in the...
Xia, Yuannan, Morgan, Richard, Schildkraut, Ira, Van Etten, James L.
A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the...
Narva, Kenneth E., Wendell, Douglas L., Skrdla, Merri P., Van Etten, James L.
The gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A was cloned and expressed in E. coli plasmid pUC8.Plasmid (pNC-1A.14.8) encoded M.CviBIII methylates adenine in TCGA...
Xia, Yuannan, Burbank, Dwight E., Uher, Lothar, Rabussay, Dietmar, Van Etten, James L.
A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease...
DNA Methyltransferase Induced by PBCV-1 Virus Infection of a Chlorella-Like Green Alga (1986)
Xia, Yuannan, Van Etten, James L.
A DNA methyltransferase was isolated from a eucaryotic, Chlorella-like green alga infected with the virus PBCV-1. The enzyme recognized the sequence GATC and methylated deoxyadenosine solely in GATC...
Xia, Yuannan, Burbank, Dwight, Uher, Lothar, Rabussay, Dietrmar, Van Etten, James L.
An enzyme was isolated from a eucaryotic, Chlorella-lie green alga infected with the virus PBCV-1 which exhibits type II restriction endonuclease activity. The enzyme recognized the sequence GATC and...
Xia, Yunnan, Burbank, Dwight E., Van Etten, James L.
A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and...
Russo, Geraldine, Van Etten, James L.
A development-specific protein (SSP) makes up about 35 to 40% of the total protein in sclerotia of the fungus Sclerotinia sclerotiorum. The protein consists of three charge isomers, with one isomer...
A Survey for Viruses from Fresh Water That Infect a Eucaryotic Chlorella-Like Green Alga (1985)
Van Etten, James L., Van Etten, Cecil H., Johnson, Jeffrey, Burbank, Dwight
Viruses which formed plaques on lawns of a eucaryotic, chlorella-like green alga were detected in 37% of the 35 freshwater samples surveyed. Virus populations, monitored in seven locations,...
DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga (1985)
Van Etten, James L., Schuster, Anne M., Girton, Lois, Burbank, Dwight E., Swinton, David, Hattman, Stanley
The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six...
Petersen, Gary, Dahlberg, Kurt, Van Etten, James L.
Muiridin, a spore-specific protein of the fungus Botryodiplodia theobromae, comprises about 25% of the mature pycnidiospore protein. It has an apparent molecular weight of 16,000 to 17,000 and is...
In Vitro Translation of the Three Bacteriophage φ6 RNAs (1980)
Cuppels, Diane A., Van Etten, James L., Burbank, Dwight E., Lane, Leslie C., Vidaver, Anne M.
In vitro translation of the three single-stranded RNAs transcribed in vitro by bacteriophage φ6 RNA polymerase revealed that the large RNA codes for phage proteins P1, P2, P4, and P7, the medium RNA...
Semiconservative Synthesis of Single-Stranded RNA by Bacteriophage φ6 RNA Polymerase (1980)
Van Etten, James L., Burbank, Dwight E., Cuppels, Diane A., Lane, Leslie C., Vidaver, Anne M.
The RNA polymerase in the nucleocapsid of seudomonas phaseolicola bacteriophage φ6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a...
Van Etten, James L., Freer, S. N., McCune, B. K.
Approximately 23% of the protein isolated from dormant spores of Botryodiplodia theobromae consisted of a single polypeptide; the polypeptide is probably degraded during germination.
Simple Procedure for Disruption of Fungal Spores (1978)
Van Etten, James L., Freer, Shelby
A simple, inexpensive method for disrupting dormant fungal spores is described.
Absence of Spermine in Filamentous Fungi (1977)
Nickerson, Kenneth, Dunkle, Larry D., Van Etten, James L.
Polyamines were examined in several yeasts and filamentous fungi. Whereas putrescine, spermidine, and spennine were present in the yeasts, spernine was not detected in any of the filamentous fungi.
Comparative Properties of Bacteriophage φ6 and φ6 Nucleocapsid (1976)
Van Etten, James L., Lane, Les, Gonzalez, Carlos, Partridge, James, Vidaver, Anne M.
Nonionic detergent treatments released a nucleocapsid from the enveloped bacteriophage φ6. The nucleocapsid sedimented at nearly the same rate as the whole phage in sucrose density gradients, but...
Intermediates in the Biosynthesis of Double-Stranded Ribonucleic Acids of Bacteriophage φ6 (1975)
Coplin, D. L., Van Etten, James L., Koski, James A., Vidaver, Anne M.
Pseudomonas phaseolicola infected with bacteriophage φ6 synthesized all three viral double-stranded RNA segments, three single-stranded RNAs, and three replicative intermediate-like RNAs in the...
Van Etten, James L., Vidaver, Anne M., Koski, R. K., Burnett, J. P.
The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage φ6 were isolated and shown to have similar melting temperatures and base compositions. RNA: RNA hybridization...
Physiological and Morphological Correlation of Rhizopus stolonifer Spore Germination (1974)
Van Etten, James L., St. Julian, Grant
Sporangiospores of Rhizopus stolonifer were examined at various stages of germination by scanning electron and phase-contrast microscopy. These observations were correlated with changes in spore dry...
RNA Polymerase Activity Associated with Bacteriophage φ6 (1973)
Van Etten, James L., Vidaver, Anne M., Koski, R. K., Semancik, J. S.
The Pseudomonas phaseolicola bacteriophage φ6 incorporated labeled UTP into an acid-insoluble precipitate. Incorporation was dependent on the presence of manganese acetate, ATP, GTP, CTP, and a...
Gong, Cheng-Shung, Dunkle, Larry, Van Etten, James L.
Deoxyribonucleic acid (DNA)-dependent DNA polymerase was purified several hundredfold from germinated and ungerminated spores of the fungus Rhizopus stolonifer. The partially purified enzymes from...
Bacteriophage φ6: a Lipid-Containing Virus of Pseudomonas phaseolicola (1973)
Vidaver, Anne M., Koski, R. K., Van Etten, James L.
The purification and properties of a lipid-containing bacteriophage, φ 6, are described. The phage contains a lipid envelope which is probably essential for infection. Infectivity of φ 6 was lost...
Van Etten, James L., Roker, Henry, Davies, Eric
The preformed messenger ribonucleic acid in Botryodiplodia theobromae spores directs the synthesis of several relatively stable polypeptides.
Preparation of Biologically Active Ribosomal Subunits from Fungal Spores (1971)
Ribosomal subunits were prepared from fungal spore ribosomes and reassociated to yield biologically active ribosomes.
Van Etten, James L., Koski, R. Kent, El-Olemy, Mahmoud
Transfer ribonucleic acid (tRNA) fractions isolated from germinated and ungerminated spores of Botryodiplodia theobromae and Rhizopus stolonifer had acceptor activity for all 20 amino acids commonly...
Van Etten, James L., Brambl, Robert
The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The...
Van Etten, James L., Molitoris, H. Peter, Gottlieb, David
The rate of respiration of Rhizoctonia solani and Sclerotium bataticola decreased with age. This decrease in respiratory rate might be produced by a decrease in the specific activity of one or more...
Gottlieb, David, Van Etten, James L.
The chemical composition of the mycelium of Rhizoctonia solani and Sclerotium bataticola was determined in cells of various ages. The percentage, per unit of dry weight, of soluble amino nitrogen,...
Gottlieb, David, Van Etten, James L.
Changes in the lipid constituents of Penicillium atrovenetum were studied during the growth and development of this fungus. The stages in development, as measured by the dry weight, were divided into...
Gottlieb, David, Van Etten, James L.
Changes in the biochemical constituents of cells were studied during the growth and development of Penicillium atrovenetum. Growth of the fungus, as measured by the dry weight, could be divided into...
The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus
Nandhagopal, Narayanasamy, Simpson, Alan A., Gurnon, James R., Yan, Xiadong, Baker, Timothy S., Graves, Michael V., ...
Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a glycoprotein coat consisting of pseudohexagonal arrays of...
Roberts, Richard J., Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S., Bickle, Thomas A., Bitinaite, Jurate, ...
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different...
Characteristics of Deoxyribonucleic Acid Polymerase Isolated from Spores of Rhizopus stolonifer1
Gong, Cheng-Shung, Dunkle, Larry D., Van Etten, James L.
Deoxyribonucleic acid (DNA)-dependent DNA polymerase was purified several hundredfold from germinated and ungerminated spores of the fungus Rhizopus stolonifer. The partially purified enzymes from...
Van Etten, James L., Koski, R. Kent, El-Olemy, Mahmoud M.
Transfer ribonucleic acid (tRNA) fractions isolated from germinated and ungerminated spores of Botryodiplodia theobromae and Rhizopus stolonifer had acceptor activity for all 20 amino acids commonly...
Van Etten, James L., Roker, Henry R., Davies, Eric
The preformed messenger ribonucleic acid in Botryodiplodia theobromae spores directs the synthesis of several relatively stable polypeptides
Gottlieb, David, Van Etten, James L.
Gottlieb, David (University of Illinois, Urbana), and James L. Van Etten. Biochemical changes during the growth of fungi. I. Nitrogen compounds and carbohydrate changes in Penicillium atrovenetum. J....
Preparation of Biologically Active Ribosomal Subunits from Fungal Spores1
Ribosomal subunits were prepared from fungal spore ribosomes and reassociated to yield biologically active ribosomes.
Physiological and Morphological Correlation of Rhizopus stolonifer Spore Germination1
Van Etten, James L., Bulla, Lee A., St. Julian, Grant
Sporangiospores of Rhizopus stolonifer were examined at various stages of germination by scanning electron and phase-contrast microscopy. These observations were correlated with changes in spore dry...
Van Etten, James L., Gottlieb, David
Van Etten, James L. (University of Illinois, Urbana), and David Gottlieb. Biochemical changes during the growth of fungi. II. Ergosterol and fatty acids in Penicillium atrovenetum. J. Bacteriol....
Changes in Fungi with Age I. Chemical Composition of Rhizoctonia solani and Sclerotium bataticola1
Gottlieb, David, Van Etten, James L.
Gottlieb, David (University of Illinois, Urbana), and James L. Van Etten. Changes in fungi with age. I. Chemical composition of Rhizoctonia solani and Sclerotium bataticola. J. Bacteriol....
Van Etten, James L., Molitoris, H. Peter, Gottlieb, David
Van Etten, James L. (University of Illinois, Urbana), H. Peter Molitoris, and David Gottlieb. Changes in fungi with age. II. Respiration and respiratory enzymes of Rhizoctonia solani and Sclerotium...
Viruses of symbiotic Chlorella-like algae isolated from Paramecium bursaria and Hydra viridis
Van Etten, James L., Meints, Russel H., Kuczmarski, Daniel, Burbank, Dwight E., Lee, Kit
We previously reported that isolation of symbiotic Chlorella-like algae from the Florida strain of Hydra viridis induced replication of a virus (designated HVCV-1) in the algae. We now report that...
Small potassium ion channel proteins encoded by chlorella viruses
Kang, Ming, Moroni, Anna, Gazzarrini, Sabrina, DiFrancesco, Dario, Thiel, Gerhard, Severino, Maria, ...
Kcv, a 94-aa protein encoded by Paramecium bursaria chlorella virus 1, is the smallest known protein to form a functional potassium ion channel and basically corresponds to the “pore module” of...
Chan, Siu-hong, Zhu, Zhenyu, Van Etten, James L., Xu, Shuang-yong
The cloning and expression of the CviPII DNA nicking and modification system encoded by chlorella virus NYs-1 is described. The system consists of a co-linear MTase encoding gene (cviPIIM) and a...
Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima
Zhang, Yuanzheng, Moriyama, Hideaki, Homma, Kohei, Van Etten, James L.
A putative deoxyuridine triphosphatase (dUTPase) gene from chlorella virus PBCV-1 was cloned, and the recombinant protein was expressed in Escherichia coli. The recombinant protein has dUTPase...
Chlorella virus MT325 encodes water and potassium channels that interact synergistically
Gazzarrini, Sabrina, Kang, Ming, Epimashko, Svetlana, Van Etten, James L., Dainty, Jack, Thiel, Gerhard, ...
Fast and selective transport of water through cell membranes is facilitated by water channels. Water channels belonging to the major intrinsic proteins (MIPs) family have been found in all three...
Frohns, Florian, Käsmann, Anja, Kramer, Detlef, Schäfer, Britta, Mehmel, Mario, Kang, Ming, ...
Previous studies have established that chlorella viruses encode K+ channels with different structural and functional properties. In the current study, we exploit the different sensitivities of these...
Virion-Associated Restriction Endonucleases of Chloroviruses
Agarkova, Irina V., Dunigan, David D., Van Etten, James L.
Chloroviruses are large, double-stranded-DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae. The prototype of the genus is Paramecium bursaria chlorella virus 1...
The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus
Nandhagopal, Narayanasamy, Simpson, Alan A., Gurnon, James R., Yan, Xiadong, Baker, Timothy S., Graves, Michael V., ...
Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a glycoprotein coat consisting of pseudohexagonal arrays of...
Roberts, Richard J., Belfort, Marlene, Bestor, Timothy, Bhagwat, Ashok S., Bickle, Thomas A., Bitinaite, Jurate, ...
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different...
Characteristics of Deoxyribonucleic Acid Polymerase Isolated from Spores of Rhizopus stolonifer1
Gong, Cheng-Shung, Dunkle, Larry D., Van Etten, James L.
Deoxyribonucleic acid (DNA)-dependent DNA polymerase was purified several hundredfold from germinated and ungerminated spores of the fungus Rhizopus stolonifer. The partially purified enzymes from...
Van Etten, James L., Koski, R. Kent, El-Olemy, Mahmoud M.
Transfer ribonucleic acid (tRNA) fractions isolated from germinated and ungerminated spores of Botryodiplodia theobromae and Rhizopus stolonifer had acceptor activity for all 20 amino acids commonly...
Van Etten, James L., Roker, Henry R., Davies, Eric
The preformed messenger ribonucleic acid in Botryodiplodia theobromae spores directs the synthesis of several relatively stable polypeptides
Gottlieb, David, Van Etten, James L.
Gottlieb, David (University of Illinois, Urbana), and James L. Van Etten. Biochemical changes during the growth of fungi. I. Nitrogen compounds and carbohydrate changes in Penicillium atrovenetum. J....
Preparation of Biologically Active Ribosomal Subunits from Fungal Spores1
Ribosomal subunits were prepared from fungal spore ribosomes and reassociated to yield biologically active ribosomes.
Physiological and Morphological Correlation of Rhizopus stolonifer Spore Germination1
Van Etten, James L., Bulla, Lee A., St. Julian, Grant
Sporangiospores of Rhizopus stolonifer were examined at various stages of germination by scanning electron and phase-contrast microscopy. These observations were correlated with changes in spore dry...
Van Etten, James L., Gottlieb, David
Van Etten, James L. (University of Illinois, Urbana), and David Gottlieb. Biochemical changes during the growth of fungi. II. Ergosterol and fatty acids in Penicillium atrovenetum. J. Bacteriol....
Changes in Fungi with Age I. Chemical Composition of Rhizoctonia solani and Sclerotium bataticola1
Gottlieb, David, Van Etten, James L.
Gottlieb, David (University of Illinois, Urbana), and James L. Van Etten. Changes in fungi with age. I. Chemical composition of Rhizoctonia solani and Sclerotium bataticola. J. Bacteriol....
Van Etten, James L., Molitoris, H. Peter, Gottlieb, David
Van Etten, James L. (University of Illinois, Urbana), H. Peter Molitoris, and David Gottlieb. Changes in fungi with age. II. Respiration and respiratory enzymes of Rhizoctonia solani and Sclerotium...
Viruses of symbiotic Chlorella-like algae isolated from Paramecium bursaria and Hydra viridis
Van Etten, James L., Meints, Russel H., Kuczmarski, Daniel, Burbank, Dwight E., Lee, Kit
We previously reported that isolation of symbiotic Chlorella-like algae from the Florida strain of Hydra viridis induced replication of a virus (designated HVCV-1) in the algae. We now report that...
Small potassium ion channel proteins encoded by chlorella viruses
Kang, Ming, Moroni, Anna, Gazzarrini, Sabrina, DiFrancesco, Dario, Thiel, Gerhard, Severino, Maria, ...
Kcv, a 94-aa protein encoded by Paramecium bursaria chlorella virus 1, is the smallest known protein to form a functional potassium ion channel and basically corresponds to the “pore module” of...
Chan, Siu-hong, Zhu, Zhenyu, Van Etten, James L., Xu, Shuang-yong
The cloning and expression of the CviPII DNA nicking and modification system encoded by chlorella virus NYs-1 is described. The system consists of a co-linear MTase encoding gene (cviPIIM) and a...
Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima
Zhang, Yuanzheng, Moriyama, Hideaki, Homma, Kohei, Van Etten, James L.
A putative deoxyuridine triphosphatase (dUTPase) gene from chlorella virus PBCV-1 was cloned, and the recombinant protein was expressed in Escherichia coli. The recombinant protein has dUTPase...
Frohns, Florian, Käsmann, Anja, Kramer, Detlef, Schäfer, Britta, Mehmel, Mario, Kang, Ming, ...
Previous studies have established that chlorella viruses encode K+ channels with different structural and functional properties. In the current study, we exploit the different sensitivities of these...
Chlorella virus MT325 encodes water and potassium channels that interact synergistically
Gazzarrini, Sabrina, Kang, Ming, Epimashko, Svetlana, Van Etten, James L., Dainty, Jack, Thiel, Gerhard, ...
Fast and selective transport of water through cell membranes is facilitated by water channels. Water channels belonging to the major intrinsic proteins (MIPs) family have been found in all three...
Virion-Associated Restriction Endonucleases of Chloroviruses
Agarkova, Irina V., Dunigan, David D., Van Etten, James L.
Chloroviruses are large, double-stranded-DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae. The prototype of the genus is Paramecium bursaria chlorella virus 1...
Zhang, Yuanzheng, Maley, Frank, Maley, Gladys F., Duncan, Garry, Dunigan, David D., Van Etten, James L.
The chlorovirus PBCV-1, like many large double-stranded DNA-containing viruses, contains several genes that encode putative proteins involved in nucleotide biosynthesis. This report describes the...
Transmembrane domain length of viral K+ channels is a signal for mitochondria targeting
Balss, Jörg, Papatheodorou, Panagiotis, Mehmel, Mario, Baumeister, Dirk, Hertel, Brigitte, Delaroque, Nicolas, ...
K+ channels operate in the plasma membrane and in membranes of organelles including mitochondria. The mechanisms and topogenic information for their differential synthesis and targeting is unknown....
Agarkova, Irina, Dunigan, David, Gurnon, James, Greiner, Timo, Barres, Julia, Thiel, Gerhard, ...
Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family of large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from the...