James M. Binley

Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques (2006)

Metzner, Karin J, Binley, James M, Gettie, Agegnehu, Marx, Preston, Nixon, Douglas F, Connor, Ruth I

Abstract Background Emergence of drug-resistant strains of human immunodeficiency virus type 1 (HIV-1) is a major obstacle to successful antiretroviral therapy (ART) in HIV-infected patients. Whether...

Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120 (2006)

Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...

Abstract During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope...

Vaccination with an Inactivated Virulent Feline Immunodeficiency Virus Engineered to Express High Levels of Env (2005)

Hosie, Margaret J., Klein, Dieter, Binley, James M., Dunsford, Thomas H., Jarrett, Oswald, Neil, James C., ...

An inactivated virus vaccine was prepared from a pathogenic isolate of feline immunodeficiency virus containing a mutation that eliminated an endocytic sorting signal in the envelope glycoprotein,...

Neutralization Sensitivity of Human Immunodeficiency Virus Type 1 Primary Isolates to Antibodies and CD4-Based Reagents Is Independent of Coreceptor Usage

Trkola, Alexandra, Ketas, Tom, KewalRamani, Vineet N., Endorf, Fred, Binley, James M., Katinger, Hermann, ...

We have investigated whether the identity of the coreceptor (CCR5, CXCR4, or both) used by primary human immunodeficiency virus type 1 (HIV-1) isolates to enter CD4+ cells influences the sensitivity...

Coreceptor Utilization by Human Immunodeficiency Virus Type 1 Is Not a Primary Determinant of Neutralization Sensitivity

Lacasse, Rachel A., Follis, Kathryn E., Moudgil, Tarsem, Trahey, Meg, Binley, James M., Planelles, Vicente, ...

We have examined the relationship between coreceptor utilization and sensitivity to neutralization in a primary isolate of human immunodeficiency virus type 1 and its T-cell line-adapted (TCLA)...

Persistent Antibody Responses but Declining Cytotoxic T-Lymphocyte Responses to Multiple Human Immunodeficiency Virus Type 1 Antigens in a Long-Term Nonprogressing Individual with a Defective p17 Proviral Sequence and No Detectable Viral RNA Expression

Binley, James M., Jin, Xia, Huang, Yaoxing, Zhang, Linqi, Cao, Yunzhen, Ho, David D., ...

Long-term nonprogressor AD-18 has been infected with human immunodeficiency virus type 1 (HIV-1) for at least 16 years. During the past 5 years, he has had undetectable levels of plasma viremia, and...

Temporal Analyses of Virus Replication, Immune Responses, and Efficacy in Rhesus Macaques Immunized with a Live, Attenuated Simian Immunodeficiency Virus Vaccine

Connor, Ruth I., Montefiori, David C., Binley, James M., Moore, John P., Bonhoeffer, Sebastian, Gettie, Agegnehu, ...

Despite evidence that live, attenuated simian immunodeficiency virus (SIV) vaccines can elicit potent protection against pathogenic SIV infection, detailed information on the replication kinetics of...

Antibody Neutralization-Resistant Primary Isolates of Human Immunodeficiency Virus Type 1

Wang, Meng, Trkola, Alexandra, Binley, James M., Purtscher, Martin, Katinger, Hermann, ...

Although typical primary isolates of human immunodeficiency virus type 1 (HIV-1) are relatively neutralization resistant, three human monoclonal antibodies and a small number of HIV-1+ human sera...

Variable-Loop-Deleted Variants of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Can Be Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits

Sanders, Rogier W., Schiffner, Linnea, Master, Aditi, Kajumo, Francis, Guo, Yong, Dragic, Tatjana, ...

We have described an oligomeric gp140 envelope glycoprotein from human immunodeficiency virus type 1 that is stabilized by an intermolecular disulfide bond between gp120 and the gp41 ectodomain,...

A Recombinant Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Complex Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits Is an Antigenic Mimic of the Trimeric Virion-Associated Structure

Binley, James M., Sanders, Rogier W., Clas, Brian, Schuelke, Norbert, Master, Aditi, Guo, Yong, ...

The few antibodies that can potently neutralize human immunodeficiency virus type 1 (HIV-1) recognize the limited number of envelope glycoprotein epitopes exposed on infectious virions. These native...

Broadly Neutralizing Antibodies Targeted to the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp41

Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...

The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...

Fine Definition of the Epitope on the gp41 Glycoprotein of Human Immunodeficiency Virus Type 1 for the Neutralizing Monoclonal Antibody 2F5

Parker, Carol E., Deterding, Leesa J., Hager-Braun, Christine, Binley, James M., Schülke, Norbert, Katinger, Hermann, ...

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), in combination with proteolytic protection assays, has been used to identify the functional epitope on human immunodeficiency...

Broadly cross-reactive HIV-1-neutralizing human monoclonal Fab selected for binding to gp120–CD4–CCR5 complexes

Moulard, Maxime, Phogat, Sanjay K., Shu, Yuuei, Labrijn, Aran F., Xiao, Xiaodong, Binley, James M., ...

HIV-1 entry into cells involves formation of a complex between gp120 of the viral envelope glycoprotein (Env), a receptor (CD4), and a coreceptor, typically CCR5. Here we provide evidence that...

Enhancing the Proteolytic Maturation of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins

Binley, James M., Sanders, Rogier W., Master, Aditi, Cayanan, Charmagne S., Wiley, Cheryl L., Schiffner, Linnea, ...

In virus-infected cells, the envelope glycoprotein (Env) precursor, gp160, of human immunodeficiency virus type 1 is cleaved by cellular proteases into a fusion-competent gp120-gp41 heterodimer in...

Oligomeric and Conformational Properties of a Proteolytically Mature, Disulfide-Stabilized Human Immunodeficiency Virus Type 1 gp140 Envelope Glycoprotein

Schülke, Norbert, Vesanen, Mika S., Sanders, Rogier W., Zhu, Ping, Lu, Min, Anselma, Deborah J., ...

We describe the further properties of a protein, designated SOS gp140, wherein the association of the gp120 and gp41 subunits of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein...

Redox-Triggered Infection by Disulfide-Shackled Human Immunodeficiency Virus Type 1 Pseudovirions

Binley, James M., Cayanan, Charmagne S., Wiley, Cheryl, Schülke, Norbert, Olson, William C., Burton, Dennis R.

We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M....

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Nature of Nonfunctional Envelope Proteins on the Surface of Human Immunodeficiency Virus Type 1

Moore, Penny L., Crooks, Emma T., Porter, Lauren, Zhu, Ping, Cayanan, Charmagne S., Grise, Henry, ...

Human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies are thought be distinguished from nonneutralizing antibodies by their ability to recognize functional gp120/gp41 envelope...

Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120

Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...

During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope (Env) with...

Vaccination with an Inactivated Virulent Feline Immunodeficiency Virus Engineered to Express High Levels of Env

Hosie, Margaret J., Klein, Dieter, Binley, James M., Dunsford, Thomas H., Jarrett, Oswald, Neil, James C., ...

An inactivated virus vaccine was prepared from a pathogenic isolate of feline immunodeficiency virus containing a mutation that eliminated an endocytic sorting signal in the envelope glycoprotein,...

Neutralization Sensitivity of Human Immunodeficiency Virus Type 1 Primary Isolates to Antibodies and CD4-Based Reagents Is Independent of Coreceptor Usage

Trkola, Alexandra, Ketas, Tom, KewalRamani, Vineet N., Endorf, Fred, Binley, James M., Katinger, Hermann, ...

We have investigated whether the identity of the coreceptor (CCR5, CXCR4, or both) used by primary human immunodeficiency virus type 1 (HIV-1) isolates to enter CD4+ cells influences the sensitivity...

Coreceptor Utilization by Human Immunodeficiency Virus Type 1 Is Not a Primary Determinant of Neutralization Sensitivity

Lacasse, Rachel A., Follis, Kathryn E., Moudgil, Tarsem, Trahey, Meg, Binley, James M., Planelles, Vicente, ...

We have examined the relationship between coreceptor utilization and sensitivity to neutralization in a primary isolate of human immunodeficiency virus type 1 and its T-cell line-adapted (TCLA)...

Persistent Antibody Responses but Declining Cytotoxic T-Lymphocyte Responses to Multiple Human Immunodeficiency Virus Type 1 Antigens in a Long-Term Nonprogressing Individual with a Defective p17 Proviral Sequence and No Detectable Viral RNA Expression

Binley, James M., Jin, Xia, Huang, Yaoxing, Zhang, Linqi, Cao, Yunzhen, Ho, David D., ...

Long-term nonprogressor AD-18 has been infected with human immunodeficiency virus type 1 (HIV-1) for at least 16 years. During the past 5 years, he has had undetectable levels of plasma viremia, and...

Temporal Analyses of Virus Replication, Immune Responses, and Efficacy in Rhesus Macaques Immunized with a Live, Attenuated Simian Immunodeficiency Virus Vaccine

Connor, Ruth I., Montefiori, David C., Binley, James M., Moore, John P., Bonhoeffer, Sebastian, Gettie, Agegnehu, ...

Despite evidence that live, attenuated simian immunodeficiency virus (SIV) vaccines can elicit potent protection against pathogenic SIV infection, detailed information on the replication kinetics of...

Antibody Neutralization-Resistant Primary Isolates of Human Immunodeficiency Virus Type 1

Wang, Meng, Trkola, Alexandra, Binley, James M., Purtscher, Martin, Katinger, Hermann, ...

Although typical primary isolates of human immunodeficiency virus type 1 (HIV-1) are relatively neutralization resistant, three human monoclonal antibodies and a small number of HIV-1+ human sera...

Variable-Loop-Deleted Variants of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Can Be Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits

Sanders, Rogier W., Schiffner, Linnea, Master, Aditi, Kajumo, Francis, Guo, Yong, Dragic, Tatjana, ...

We have described an oligomeric gp140 envelope glycoprotein from human immunodeficiency virus type 1 that is stabilized by an intermolecular disulfide bond between gp120 and the gp41 ectodomain,...

A Recombinant Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Complex Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits Is an Antigenic Mimic of the Trimeric Virion-Associated Structure

Binley, James M., Sanders, Rogier W., Clas, Brian, Schuelke, Norbert, Master, Aditi, Guo, Yong, ...

The few antibodies that can potently neutralize human immunodeficiency virus type 1 (HIV-1) recognize the limited number of envelope glycoprotein epitopes exposed on infectious virions. These native...

Broadly Neutralizing Antibodies Targeted to the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp41

Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...

The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...

Fine Definition of the Epitope on the gp41 Glycoprotein of Human Immunodeficiency Virus Type 1 for the Neutralizing Monoclonal Antibody 2F5

Parker, Carol E., Deterding, Leesa J., Hager-Braun, Christine, Binley, James M., Schülke, Norbert, Katinger, Hermann, ...

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), in combination with proteolytic protection assays, has been used to identify the functional epitope on human immunodeficiency...

Broadly cross-reactive HIV-1-neutralizing human monoclonal Fab selected for binding to gp120–CD4–CCR5 complexes

Moulard, Maxime, Phogat, Sanjay K., Shu, Yuuei, Labrijn, Aran F., Xiao, Xiaodong, Binley, James M., ...

HIV-1 entry into cells involves formation of a complex between gp120 of the viral envelope glycoprotein (Env), a receptor (CD4), and a coreceptor, typically CCR5. Here we provide evidence that...

Enhancing the Proteolytic Maturation of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins

Binley, James M., Sanders, Rogier W., Master, Aditi, Cayanan, Charmagne S., Wiley, Cheryl L., Schiffner, Linnea, ...

In virus-infected cells, the envelope glycoprotein (Env) precursor, gp160, of human immunodeficiency virus type 1 is cleaved by cellular proteases into a fusion-competent gp120-gp41 heterodimer in...

Oligomeric and Conformational Properties of a Proteolytically Mature, Disulfide-Stabilized Human Immunodeficiency Virus Type 1 gp140 Envelope Glycoprotein

Schülke, Norbert, Vesanen, Mika S., Sanders, Rogier W., Zhu, Ping, Lu, Min, Anselma, Deborah J., ...

We describe the further properties of a protein, designated SOS gp140, wherein the association of the gp120 and gp41 subunits of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein...

Redox-Triggered Infection by Disulfide-Shackled Human Immunodeficiency Virus Type 1 Pseudovirions

Binley, James M., Cayanan, Charmagne S., Wiley, Cheryl, Schülke, Norbert, Olson, William C., Burton, Dennis R.

We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M....

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Nature of Nonfunctional Envelope Proteins on the Surface of Human Immunodeficiency Virus Type 1

Moore, Penny L., Crooks, Emma T., Porter, Lauren, Zhu, Ping, Cayanan, Charmagne S., Grise, Henry, ...

Human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies are thought be distinguished from nonneutralizing antibodies by their ability to recognize functional gp120/gp41 envelope...

Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120

Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...

During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope (Env) with...

Vaccination with an Inactivated Virulent Feline Immunodeficiency Virus Engineered to Express High Levels of Env

Hosie, Margaret J., Klein, Dieter, Binley, James M., Dunsford, Thomas H., Jarrett, Oswald, Neil, James C., ...

An inactivated virus vaccine was prepared from a pathogenic isolate of feline immunodeficiency virus containing a mutation that eliminated an endocytic sorting signal in the envelope glycoprotein,...

An Affinity-Enhanced Neutralizing Antibody against the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 gp41 Recognizes an Epitope between Those of 2F5 and 4E10▿

Nelson, Josh D., Brunel, Florence M., Jensen, Richard, Crooks, Emma T., Cardoso, Rosa M. F., Wang, Meng, ...

The membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 bears the epitopes of two broadly neutralizing antibodies (Abs), 2F5 and 4E10, making it a target for...

HIV-1 Antigen–specific and –nonspecific B Cell Responses Are Sensitive to Combination Antiretroviral Therapy

Morris, Lynn, Binley, James M., Clas, Brian A., Bonhoeffer, Sebastian, Astill, Thomas P., Kost, Rhonda, ...

We studied how combination antiviral therapy affects B cell abnormalities associated with HIV-1 infection, namely elevated circulating immunoglobulin (Ig)G antibody-secreting cell (ASC) frequencies...

Profiling the Specificity of Neutralizing Antibodies in a Large Panel of Plasmas from Patients Chronically Infected with Human Immunodeficiency Virus Type 1 Subtypes B and C▿ †

Binley, James M., Lybarger, Elizabeth A., Crooks, Emma T., Seaman, Michael S., Gray, Elin, Davis, Katie L., ...

Identifying the viral epitopes targeted by broad neutralizing antibodies (NAbs) that sometimes develop in human immunodeficiency virus type 1 (HIV-1)-infected subjects should assist in the design of...