Determination of the transphosphorylation sites of Jak2 kinase. (2004)
Matsuda, Tadashi, Feng, Jian, Witthuhn, Bruce A., Sekine, Yuichi, Ihle, James N.
Janus kinases are the key enzymes involved in the initial transmission of signals in response to type I and II cytokines. Activation of the signal begins with the transphosphorylation of Jak kinases....
Determination of the transphosphorylation sites of Jak2 kinase. (2004)
Matsuda, Tadashi, Feng, Jian, Witthuhn, Bruce A., Sekine, Yuichi, Ihle, James N.
Janus kinases are the key enzymes involved in the initial transmission of signals in response to type I and II cytokines. Activation of the signal begins with the transphosphorylation of Jak kinases....
Baudino, Troy A., McKay, Catriona, Pendeville-Samain, Helene, Nilsson, Jonas A., Maclean, Kirsteen H., White, Elsie L., ...
Antiapoptotic activity of Stat5 required during terminal stages of myeloid differentiation (2000)
Kieslinger, Matthias, Woldman, Irina, Moriggl, Richard, Hofmann, Johannes, Marine, Jean-Christophe, Ihle, James N., ...
Quelle, Frederick W., Wang, JinLing, Feng, Jian, Wang, Demin, Cleveland, John L., Ihle, James N., ...
Packham, Graham, White, Elsie L., Eischen, Christine M., Yang, Hui, Parganas, Evan, Ihle, James N., ...
Identification of JAK2 as a growth hormone receptor-associated tyrosine kinase (1993)
Argetsinger, Lawrence S., Campbell, George S., Yang, Xianjie, Witthuhn, Bruce A., Silvennoinen, Olli, Ihle, James N., ...
Growth hormone receptor (GHR) forms a complex with a tyrosine kinase, suggesting involvement of a ligand-activated tyrosine kinase in intracellular signaling by growth hormone (GH). Here we identify...
Jak3 Selectively Regulates Bax and Bcl-2 Expression To Promote T-Cell Development
Wen, Renren, Wang, Demin, McKay, Catriona, Bunting, Kevin D., Marine, Jean-Christophe, Vanin, Elio F., ...
Jak3-deficient mice display vastly reduced numbers of lymphoid cells. Thymocytes and peripheral T cells from Jak3-deficient mice have a high apoptotic index, suggesting that Jak3 provides survival...
Gadd45γ Is Dispensable for Normal Mouse Development and T-Cell Proliferation
Hoffmeyer, Angelika, Piekorz, Roland, Moriggl, Richard, Ihle, James N.
Gadd45γ, a family member of the growth arrest and DNA damage-inducible gene family 45 (Gadd45), is strongly induced by interleukin-2 (IL-2) in peripheral T cells. While in most tissues all Gadd45...
Piekorz, Roland P., Hoffmeyer, Angelika, Duntsch, Christopher D., McKay, Catriona, Nakajima, Hideaki, Sexl, Veronika, ...
TACC3 is a centrosomal/mitotic spindle-associated protein that is highly expressed in a cell cycle-dependent manner in hematopoietic lineage cells. During embryonic development, TACC3 is expressed in...
Nakajima, Hideaki, Brindle, Paul K., Handa, Makoto, Ihle, James N.
Signal transducers and activators of transcription (STATs) play a central role in cytokine signaling. Activating and repressing gene transcription is a dynamic process involving chromatin remodeling...
Zhao, Shengming, Zoller, Karen, Masuko, Masayoshi, Rojnuckarin, Ponlapat, Yang, Xuexian O., Parganas, Evan, ...
Defining signals that can support the self-renewal of multipotential hemopoietic progenitor cells (MHPCs) is pertinent to understanding leukemogenesis and may be relevant to developing stem...
Carpino, Nick, Kobayashi, Ryuji, Zang, Heesuk, Takahashi, Yutaka, Jou, Shiann-Tarrng, Feng, Jian, ...
In a screen for proteins that interact with Jak2, we identified a previously uncharacterized 70-kDa protein and cloned the corresponding cDNA. The predicated sequence indicates that p70 contains an...
Jou, Shiann-Tarng, Carpino, Nick, Takahashi, Yutaka, Piekorz, Roland, Chao, Jyh-Rong, Carpino, Neena, ...
Many receptor and nonreceptor tyrosine kinases activate phosphoinositide 3-kinases (PI3Ks). To assess the role of the δ isoform of the p110 catalytic subunit of PI3Ks, we derived enzyme-deficient...
SOCS3: an essential regulator of LIF receptor signaling in trophoblast giant cell differentiation
Takahashi, Yutaka, Carpino, Nick, Cross, James C., Torres, Miguel, Parganas, Evan, Ihle, James N.
Suppressor of cytokine signaling 3 (SOCS3) binds cytokine receptors and thereby suppresses cytokine signaling. Deletion of SOCS3 causes an embryonic lethality that is rescued by a tetraploid rescue...
Zang, Heesuk, Sato, Ken, Nakajima, Hideaki, McKay, Catriona, Ney, Paul A., Ihle, James N.
The erythropoietin receptor (EpoR) is required for the proliferation and survival of committed erythroid lineage cells. Previous studies have utilized receptor mutations to show the requirement for...
c-Myc is essential for vasculogenesis and angiogenesis during development and tumor progression
Baudino, Troy A., McKay, Catriona, Pendeville-Samain, Helene, Nilsson, Jonas A., Maclean, Kirsteen H., White, Elsie L., ...
c-Myc promotes cell growth and transformation by ill-defined mechanisms. c-myc−/− mice die by embryonic day 10.5 (E10.5) with defects in growth and in cardiac and neural development. Here we...
Wang, Demin, Moriggl, Richard, Stravopodis, Dimitrios, Carpino, Nick, Marine, Jean–Christophe, Teglund, Stephan, ...
Cytokines induce the tyrosine phosphorylation and associated activation of signal transducers and acti– vators of transcription (Stat). The mechanisms by which this response is terminated are...
Antiapoptotic activity of Stat5 required during terminal stages of myeloid differentiation
Kieslinger, Matthias, Woldman, Irina, Moriggl, Richard, Hofmann, Johannes, Marine, Jean-Christophe, Ihle, James N., ...
Stat5 is activated by multiple receptors of hematopoietic cytokines. To study its role during hematopoiesis, we have generated primary chicken myeloblasts expressing different dominant-negative (dn)...
Quelle, Frederick W., Wang, JinLing, Feng, Jian, Wang, Demin, Cleveland, John L., Ihle, James N., ...
Exposure of hematopoietic progenitors to γ-irradiation (IR) induces p53-dependent apoptosis and a p53-independent G2/M cell cycle arrest. These responses to DNA-damage can be inhibited by treatment...
Packham, Graham, White, Elsie L., Eischen, Christine M., Yang, Hui, Parganas, Evan, Ihle, James N., ...
Bcl-2 family proteins are key regulators of apoptosis and function as cell death antagonists (e.g., Bcl-2, Bcl-XL, and Mcl-1) or agonists (e.g., Bax, Bad, and Bak). Here we report that among the...
Evidence for a Stable Intermediate in Leukemia Virus Activation in AKR Mouse Embryo Cells
Ihle, James N., Kenney, Francis T., Tennant, Raymond W.
Analysis of the requirement for serum in the activation of the endogenous leukemia virus expression in AKR mouse embryo cells by 5-iododeoxyuridine shows that activation can be dissociated into two...
Absence of an Essential Role for Thymic Stromal Lymphopoietin Receptor in Murine B-Cell Development
Carpino, Nick, Thierfelder, William E., Chang, Ming-shi, Saris, Chris, Turner, Steven J., Ziegler, Steven F., ...
The murine cytokine thymic stromal lymphopoietin (TSLP) supports the development of B220+ IgM+ immature B cells and induces thymocyte proliferation in vitro. Human TSLP, by contrast, activates CD11c+...
Effects of Polyadenylic Acids on Functions of Murine RNA Tumor Viruses
Tennant, Raymond W., Farrelly, James G., Ihle, James N., Pal, B. C., Kenney, Francis T., Brown, Arthur
Single-stranded polyribonucleotides, which competitively inhibit murine RNA tumor virus reverse transcriptase in vitro, were tested as inhibitors of various virus functions in cell culture. The...
Oroszlan, Stephen, Henderson, Louis E., Stephenson, John R., Copeland, Terry D., Long, Cedric W., Ihle, James N., ...
The amino- and carboxyl-terminal amino acid sequences of proteins (p10, p12, p15, and p30) coded by the gag gene of Rauscher and AKR murine leukemia viruses were determined. Among these proteins, p15...
Schuendeln, Michael M., Piekorz, Roland P., Wichmann, Christian, Lee, Youngsoo, McKinnon, Peter J., Boyd, Kelli, ...
TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. In vivo, the TACC2 gene is expressed in...
Jak3 Selectively Regulates Bax and Bcl-2 Expression To Promote T-Cell Development
Wen, Renren, Wang, Demin, McKay, Catriona, Bunting, Kevin D., Marine, Jean-Christophe, Vanin, Elio F., ...
Jak3-deficient mice display vastly reduced numbers of lymphoid cells. Thymocytes and peripheral T cells from Jak3-deficient mice have a high apoptotic index, suggesting that Jak3 provides survival...
Gadd45γ Is Dispensable for Normal Mouse Development and T-Cell Proliferation
Hoffmeyer, Angelika, Piekorz, Roland, Moriggl, Richard, Ihle, James N.
Gadd45γ, a family member of the growth arrest and DNA damage-inducible gene family 45 (Gadd45), is strongly induced by interleukin-2 (IL-2) in peripheral T cells. While in most tissues all Gadd45...
Piekorz, Roland P., Hoffmeyer, Angelika, Duntsch, Christopher D., McKay, Catriona, Nakajima, Hideaki, Sexl, Veronika, ...
TACC3 is a centrosomal/mitotic spindle-associated protein that is highly expressed in a cell cycle-dependent manner in hematopoietic lineage cells. During embryonic development, TACC3 is expressed in...
Nakajima, Hideaki, Brindle, Paul K., Handa, Makoto, Ihle, James N.
Signal transducers and activators of transcription (STATs) play a central role in cytokine signaling. Activating and repressing gene transcription is a dynamic process involving chromatin remodeling...
Zhao, Shengming, Zoller, Karen, Masuko, Masayoshi, Rojnuckarin, Ponlapat, Yang, Xuexian O., Parganas, Evan, ...
Defining signals that can support the self-renewal of multipotential hemopoietic progenitor cells (MHPCs) is pertinent to understanding leukemogenesis and may be relevant to developing stem...
Carpino, Nick, Kobayashi, Ryuji, Zang, Heesuk, Takahashi, Yutaka, Jou, Shiann-Tarrng, Feng, Jian, ...
In a screen for proteins that interact with Jak2, we identified a previously uncharacterized 70-kDa protein and cloned the corresponding cDNA. The predicated sequence indicates that p70 contains an...
Jou, Shiann-Tarng, Carpino, Nick, Takahashi, Yutaka, Piekorz, Roland, Chao, Jyh-Rong, Carpino, Neena, ...
Many receptor and nonreceptor tyrosine kinases activate phosphoinositide 3-kinases (PI3Ks). To assess the role of the δ isoform of the p110 catalytic subunit of PI3Ks, we derived enzyme-deficient...
SOCS3: an essential regulator of LIF receptor signaling in trophoblast giant cell differentiation
Takahashi, Yutaka, Carpino, Nick, Cross, James C., Torres, Miguel, Parganas, Evan, Ihle, James N.
Suppressor of cytokine signaling 3 (SOCS3) binds cytokine receptors and thereby suppresses cytokine signaling. Deletion of SOCS3 causes an embryonic lethality that is rescued by a tetraploid rescue...
Zang, Heesuk, Sato, Ken, Nakajima, Hideaki, McKay, Catriona, Ney, Paul A., Ihle, James N.
The erythropoietin receptor (EpoR) is required for the proliferation and survival of committed erythroid lineage cells. Previous studies have utilized receptor mutations to show the requirement for...
c-Myc is essential for vasculogenesis and angiogenesis during development and tumor progression
Baudino, Troy A., McKay, Catriona, Pendeville-Samain, Helene, Nilsson, Jonas A., Maclean, Kirsteen H., White, Elsie L., ...
c-Myc promotes cell growth and transformation by ill-defined mechanisms. c-myc−/− mice die by embryonic day 10.5 (E10.5) with defects in growth and in cardiac and neural development. Here we...
Wang, Demin, Moriggl, Richard, Stravopodis, Dimitrios, Carpino, Nick, Marine, Jean–Christophe, Teglund, Stephan, ...
Cytokines induce the tyrosine phosphorylation and associated activation of signal transducers and acti– vators of transcription (Stat). The mechanisms by which this response is terminated are...
Antiapoptotic activity of Stat5 required during terminal stages of myeloid differentiation
Kieslinger, Matthias, Woldman, Irina, Moriggl, Richard, Hofmann, Johannes, Marine, Jean-Christophe, Ihle, James N., ...
Stat5 is activated by multiple receptors of hematopoietic cytokines. To study its role during hematopoiesis, we have generated primary chicken myeloblasts expressing different dominant-negative (dn)...
Quelle, Frederick W., Wang, JinLing, Feng, Jian, Wang, Demin, Cleveland, John L., Ihle, James N., ...
Exposure of hematopoietic progenitors to γ-irradiation (IR) induces p53-dependent apoptosis and a p53-independent G2/M cell cycle arrest. These responses to DNA-damage can be inhibited by treatment...
Packham, Graham, White, Elsie L., Eischen, Christine M., Yang, Hui, Parganas, Evan, Ihle, James N., ...
Bcl-2 family proteins are key regulators of apoptosis and function as cell death antagonists (e.g., Bcl-2, Bcl-XL, and Mcl-1) or agonists (e.g., Bax, Bad, and Bak). Here we report that among the...
Evidence for a Stable Intermediate in Leukemia Virus Activation in AKR Mouse Embryo Cells
Ihle, James N., Kenney, Francis T., Tennant, Raymond W.
Analysis of the requirement for serum in the activation of the endogenous leukemia virus expression in AKR mouse embryo cells by 5-iododeoxyuridine shows that activation can be dissociated into two...
Absence of an Essential Role for Thymic Stromal Lymphopoietin Receptor in Murine B-Cell Development
Carpino, Nick, Thierfelder, William E., Chang, Ming-shi, Saris, Chris, Turner, Steven J., Ziegler, Steven F., ...
The murine cytokine thymic stromal lymphopoietin (TSLP) supports the development of B220+ IgM+ immature B cells and induces thymocyte proliferation in vitro. Human TSLP, by contrast, activates CD11c+...
Effects of Polyadenylic Acids on Functions of Murine RNA Tumor Viruses
Tennant, Raymond W., Farrelly, James G., Ihle, James N., Pal, B. C., Kenney, Francis T., Brown, Arthur
Single-stranded polyribonucleotides, which competitively inhibit murine RNA tumor virus reverse transcriptase in vitro, were tested as inhibitors of various virus functions in cell culture. The...
Oroszlan, Stephen, Henderson, Louis E., Stephenson, John R., Copeland, Terry D., Long, Cedric W., Ihle, James N., ...
The amino- and carboxyl-terminal amino acid sequences of proteins (p10, p12, p15, and p30) coded by the gag gene of Rauscher and AKR murine leukemia viruses were determined. Among these proteins, p15...
Schuendeln, Michael M., Piekorz, Roland P., Wichmann, Christian, Lee, Youngsoo, McKinnon, Peter J., Boyd, Kelli, ...
TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. In vivo, the TACC2 gene is expressed in...
Two Domains of the Erythropoietin Receptor Are Sufficient for Jak2 Binding/Activation and Function▿
Pelletier, Stéphane, Gingras, Sébastien, Funakoshi-Tago, Megumi, Howell, Sherié, Ihle, James N.
Biochemical and genetic studies have shown that Jak2 is an essential component of EpoR signal transduction which is required for normal erythropoiesis. However, whether Jak2 is the sole direct...
Role of erythropoietin receptor signaling in Friend virus-induced erythroblastosis and polycythemia
Zhang, Ji, Randall, Mindy S., Loyd, Melanie R., Li, Weimin, Schweers, Rachel L., Persons, Derek A., ...
Friend virus is an acutely oncogenic retrovirus that causes erythroblastosis and polycythemia in mice. Previous studies suggested that the Friend virus oncoprotein, gp55, constitutively activates the...
Characterization of a Family of Novel Cysteine- Serine-Rich Nuclear Proteins (CSRNP)
Gingras, Sébastien, Pelletier, Stéphane, Boyd, Kelli, Ihle, James N.
Gene array analysis has been widely used to identify genes induced during T cell activation. Our studies identified an immediate early gene that is strongly induced in response to IL-2 in mouse T...
Funakoshi-Tago, Megumi, Pelletier, Stephane, Matsuda, Tadashi, Parganas, Evan, Ihle, James N
The tyrosine kinase, Janus kinase-2 (Jak2), plays a pivotal role in signal transduction through a variety of cytokine receptors, including the receptor for erythropoietin (Epo). Although the...
Jak2 FERM Domain Interaction with the Erythropoietin Receptor Regulates Jak2 Kinase Activity▿
Funakoshi-Tago, Megumi, Pelletier, Stéphane, Moritake, Hiroshi, Parganas, Evan, Ihle, James N.
Janus kinases are essential for signal transduction by a variety of cytokine receptors and when inappropriately activated can cause hematopoietic disorders and oncogenesis. Consequently, it can be...