James P. Di Santo

Loss of the pro-apoptotic BH3-only Bcl-2 family member Bim sustains B lymphopoiesis in the absence of IL-7 (2009)

Huntington, Nicholas D., Labi, Verena, Cumano, Ana, Vieira, Paulo, Strasser, Andreas, Villunger, Andreas, ...

IL-7 is pivotal for B cell development. Proteins of the Bcl-2 family are essential regulators of lymphocyte survival. Particularly, the pro-apoptotic BH3-only members Bim and Puma mediate lymphocyte...

In vivo equilibrium of proinflammatory IL-17+ and regulatory IL-10+ Foxp3+ RORgamma t+ T cells (2008)

Lochner, Matthias, Peduto, Lucie, Cherrier, Marie, Sawa, Shinichiro, Langa, Francina, Varona, Rosa, ...

The nuclear hormone receptor retinoic acid receptor-related orphan receptor gamma t (RORgamma t) is required for the generation of T helper 17 cells expressing the proinflammatory cytokine...

NKG2D triggers cytotoxicity in mouse NK cells lacking DAP12 or Syk family kinases. (2003)

Zompi, Simona, Hamerman, Jessica A, Ogasawara, Kouetsu, Schweighoffer, Edina, Tybulewicz, Victor L J, Di Santo, James P, ...

In activated mouse natural killer (NK) cells, the NKG2D receptor associates with two intracellular adaptors, DAP10 and DAP12, which trigger phosphatidyl inositol 3 kinase (PI3K) and Syk family...

NKG2D triggers cytotoxicity in mouse NK cells lacking DAP12 or Syk family kinases (2003)

Zompi, Simona, Hamerman, Jessica A, Ogasawara, Kouetsu, Schweighoffer, Edina, Tybulewicz, Victor L J, Di Santo, James P, ...

In activated mouse natural killer (NK) cells, the NKG2D receptor associates with two intracellular adaptors, DAP10 and DAP12, which trigger phosphatidyl inositol 3 kinase (PI3K) and Syk family...

Differential requirement of the cytoplasmic subregions of γc chain in T cell development and function

Tsujino, Shiho, Di Santo, James P., Takaoka, Akinori, McKernan, Teresa L., Noguchi, Shigeru, Taya, Choji, ...

The common cytokine receptor γ chain (γc), a shared component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15, is critical for the development and function of lymphocytes. The cytoplasmic...

Stress-Induced ClpP Serine Protease of Listeria monocytogenes Is Essential for Induction of Listeriolysin O-Dependent Protective Immunity

Gaillot, Olivier, Bregenholt, Søren, Jaubert, Francis, Di Santo, James P., Berche, Patrick

The stress-induced protease ClpP is required for virulence of the facultative intracellular pathogen Listeria monocytogenes. We previously found that in the absence of ClpP, the virulence of this...

IL-15 availability conditions homeostasis of peripheral natural killer T cells

Ranson, Thomas, Vosshenrich, Christian A. J., Corcuff, Erwan, Richard, Odile, Laloux, Veronique, Lehuen, Agnès, ...

Steady-state numbers of peripheral lymphocyte are tightly controlled. For conventional T cells, signals delivered through the interaction of the T cell receptor (TCR) with antigen-loaded MHC...

Role of an Intact Splenic Microarchitecture in Early Lymphocytic Choriomeningitis Virus Production

Müller, Stefan, Hunziker, Lukas, Enzler, Susanne, Bühler-Jungo, Myriam, Di Santo, James P., Zinkernagel, Rolf M., ...

An acute infection with lymphocytic choriomeningitis virus (LCMV) is efficiently controlled by the cytotoxic-T-cell (CTL) response of the host, and LCMV titers in the spleen and peripheral solid...

IκBα/IκBε deficiency reveals that a critical NF-κB dosage is required for lymphocyte survival

Goudeau, Bertrand, Huetz, François, Samson, Sandrine, Di Santo, James P., Cumano, Ana, Beg, Amer, ...

In most cells, the NF-κB transcription factor is sequestered in the cytoplasm by interaction with inhibitory proteins, the IκBs. Here, we show that combined IκBα/IκBε deficiency in mice leads...

Interplay between Alpha/Beta and Gamma Interferons with B, T, and Natural Killer Cells in the Defense against Herpes Simplex Virus Type 1

Vollstedt, Sabine, Arnold, Susi, Schwerdel, Cornelia, Franchini, Marco, Alber, Gottfried, Di Santo, James P., ...

The essential components of the immune system that control primary and chronic infection with herpes simplex virus type 1 (HSV-1) in mice were investigated. Infection within the first few days can be...

Pre-B cell receptor expression is necessary for thymic stromal lymphopoietin responsiveness in the bone marrow but not in the liver environment

Vosshenrich, Christian A. J., Cumano, Ana, Müller, Werner, Di Santo, James P., Vieira, Paulo

IL-7 and thymic stromal lymphopoietin (TSLP) are two major cytokines controlling murine B cell development. IL-7 has been studied extensively, but only recently has it become possible to unravel the...

Differential requirement of the cytoplasmic subregions of γc chain in T cell development and function

Tsujino, Shiho, Di Santo, James P., Takaoka, Akinori, McKernan, Teresa L., Noguchi, Shigeru, Taya, Choji, ...

The common cytokine receptor γ chain (γc), a shared component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15, is critical for the development and function of lymphocytes. The cytoplasmic...

Stress-Induced ClpP Serine Protease of Listeria monocytogenes Is Essential for Induction of Listeriolysin O-Dependent Protective Immunity

Gaillot, Olivier, Bregenholt, Søren, Jaubert, Francis, Di Santo, James P., Berche, Patrick

The stress-induced protease ClpP is required for virulence of the facultative intracellular pathogen Listeria monocytogenes. We previously found that in the absence of ClpP, the virulence of this...

IL-15 availability conditions homeostasis of peripheral natural killer T cells

Ranson, Thomas, Vosshenrich, Christian A. J., Corcuff, Erwan, Richard, Odile, Laloux, Veronique, Lehuen, Agnès, ...

Steady-state numbers of peripheral lymphocyte are tightly controlled. For conventional T cells, signals delivered through the interaction of the T cell receptor (TCR) with antigen-loaded MHC...

Role of an Intact Splenic Microarchitecture in Early Lymphocytic Choriomeningitis Virus Production

Müller, Stefan, Hunziker, Lukas, Enzler, Susanne, Bühler-Jungo, Myriam, Di Santo, James P., Zinkernagel, Rolf M., ...

An acute infection with lymphocytic choriomeningitis virus (LCMV) is efficiently controlled by the cytotoxic-T-cell (CTL) response of the host, and LCMV titers in the spleen and peripheral solid...

IκBα/IκBε deficiency reveals that a critical NF-κB dosage is required for lymphocyte survival

Goudeau, Bertrand, Huetz, François, Samson, Sandrine, Di Santo, James P., Cumano, Ana, Beg, Amer, ...

In most cells, the NF-κB transcription factor is sequestered in the cytoplasm by interaction with inhibitory proteins, the IκBs. Here, we show that combined IκBα/IκBε deficiency in mice leads...

Interplay between Alpha/Beta and Gamma Interferons with B, T, and Natural Killer Cells in the Defense against Herpes Simplex Virus Type 1

Vollstedt, Sabine, Arnold, Susi, Schwerdel, Cornelia, Franchini, Marco, Alber, Gottfried, Di Santo, James P., ...

The essential components of the immune system that control primary and chronic infection with herpes simplex virus type 1 (HSV-1) in mice were investigated. Infection within the first few days can be...

Pre-B cell receptor expression is necessary for thymic stromal lymphopoietin responsiveness in the bone marrow but not in the liver environment

Vosshenrich, Christian A. J., Cumano, Ana, Müller, Werner, Di Santo, James P., Vieira, Paulo

IL-7 and thymic stromal lymphopoietin (TSLP) are two major cytokines controlling murine B cell development. IL-7 has been studied extensively, but only recently has it become possible to unravel the...

γc cytokines condition the progressive differentiation of CD4+ T cells

Masse, Guillemette X., Corcuff, Erwan, Strick-Marchand, Hélène, Guy-Grand, Delphine, Tafuri-Bladt, Anna, Albert, Matthew L., ...

After their initial antigen encounter in the secondary lymphoid organs, activated T cells must receive additional signals in the peripheral tissues to fully differentiate. Here, we provide evidence...

Are Major Histocompatibility Complex Molecules Involved in the Survival of Naive CD4+ T Cells?

Grandjean, Isabelle, Duban, Livine, Bonney, Elizabeth A., Corcuff, Erwan, Di Santo, James P., Matzinger, Polly, ...

The exact role of major histocompatibility complex (MHC) molecules in the peripheral survival of naive T cells is controversial, as some studies have suggested that they are critically required...

The Common Cytokine Receptor γ Chain and the Pre-T Cell Receptor Provide Independent but Critically Overlapping Signals in Early α/β T Cell Development

Di Santo, James P., Aifantis, Iannis, Rosmaraki, Eleftheria, Garcia, Corinne, Feinberg, Jacqueline, Fehling, Hans Jörg, ...

Intracellular signals emanating from cytokine and antigen receptors are integrated during the process of intrathymic development. Still, the relative contributions of cytokine receptor signaling to...

Interferon γ Contributes to Initiation of Uterine Vascular Modification, Decidual Integrity, and Uterine Natural Killer Cell Maturation during Normal Murine Pregnancy

Ashkar, Ali A., Di Santo, James P., Croy, B. Anne

The dominant lymphocytes in human and murine implantation sites are transient, pregnancy-associated uterine natural killer (uNK) cells. These cells are a major source of interferon (IFN)-γ....

Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor

Park, Linda S., Martin, Unja, Garka, Kirsten, Gliniak, Brian, Di Santo, James P., Muller, Werner, ...

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the...

Functional Dichotomy in Natural Killer Cell Signaling: Vav1-Dependent and -Independent Mechanisms

Colucci, Francesco, Rosmaraki, Eleftheria, Bregenholt, Søren, Samson, Sandrine I., Di Bartolo, Vincenzo, Turner, Martin, ...

The product of the protooncogene Vav1 participates in multiple signaling pathways and is a critical regulator of antigen–receptor signaling in B and T lymphocytes, but its role during in vivo...

Early T Cell Receptor β Gene Expression Is Regulated by the Pre-T Cell Receptor–CD3 Complex

Aifantis, Iannis, Feinberg, Jacqueline, Fehling, Hans Jörg, Di Santo, James P., Von Boehmer, Harald

We have examined the question of whether there is an additional checkpoint in T cell development that regulates T cell receptor (TCR)-β expression in CD25+44− thymocytes by mechanisms that are...

CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells

Vosshenrich, Christian A.J., Lesjean-Pottier, Sarah, Hasan, Milena, Corcuff, Erwan, Mandelboim, Ofer, ...

Interferon-producing killer dendritic cells (IKDCs) are a recently described subset of CD11cloB220+ cells that share phenotypic and functional properties of DCs and natural killer (NK) cells (Chan,...

Myogenic cell proliferation and generation of a reversible tumorigenic phenotype are triggered by preirradiation of the recipient site

Morgan, Jennifer E., Gross, Jacqueline G., Pagel, Charles N., Beauchamp, Jonathan R., Fassati, Ariberto, Thrasher, Adrian J., ...

Environmental influences have profound yet reversible effects on the behavior of resident cells. Earlier data have indicated that the amount of muscle formed from implanted myogenic cells is greatly...

IL-15 trans-presentation promotes human NK cell development and differentiation in vivo

Huntington, Nicholas D., Legrand, Nicolas, Alves, Nuno L., Jaron, Barbara, Weijer, Kees, Plet, Ariane, ...

The in vivo requirements for human natural killer (NK) cell development and differentiation into cytotoxic effectors expressing inhibitory receptors for self–major histocompatability complex class...

Characterization of the thymic IL-7 niche in vivo

Alves, Nuno L., Goff, Odile Richard-Le, Huntington, Nicholas D., Sousa, Ana Patricia, Ribeiro, Vera S. G., Bordack, Allison, ...

The thymus represents the “cradle” for T cell development, with thymic stroma providing multiple soluble and membrane cues to developing thymocytes. Although IL-7 is recognized as an essential...