Type I signal peptidases of Gram-positive bacteria (2004)
Jongbloed, Jan D H, Tjalsma, Harold, Dubois, Jean-Yves F, Bron, Sierd, ...
Proteins that are exported from the cytoplasm to the periplasm and outer membrane of Gram-negative bacteria, or the cell wall and growth medium of Gram-positive bacteria, are generally synthesized as...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
A Proteomic View on Genome-Based Signal Peptide Predictions (2001)
Antelmann, Haike, Tjalsma, Harold, Voigt, Birgit, Ohlmeier, Steffen, Bron, Sierd, Van Dijl, Jan Maarten, ...
Tjalsma, Harold, Bolhuis, Albert, Van Roosmalen, Maarten L., Wiegert, Thomas, Schumann, Wolfgang, Broekhuizen, Cees P., ...
Van Dijl, Jan Maarten, Kutejová, Eva, Suda, Kitaru, Perečko, Dušan, Schatz, Gottfried, Suzuki, Carolyn K.
The ATP-dependent Lon protease of Saccharomyces cerevisiae mitochondria is required for selective proteolysis in the matrix, maintenance of mitochondrial DNA, and respiration-dependent growth. Lon...
Evaluation of Bottlenecks in the Late Stages of Protein Secretion in Bacillus subtilis
Bolhuis, Albert, Tjalsma, Harold, Smith, Hilde E., De Jong, Anne, Meima, Rob, Venema, Gerard, ...
Despite a high capacity for secretion of homologous proteins, the secretion of heterologous proteins by Bacillus subtilis is frequently inefficient. In the present studies, we have investigated and...
Tjalsma, Harold, Van Den Dolder, Juliëtte, Meijer, Wilfried J. J., Venema, Gerard, Bron, Sierd, Van Dijl, Jan Maarten
The gram-positive eubacterium Bacillus subtilis is the organism with the largest number of paralogous type I signal peptidases (SPases) known. These are specified both by chromosomal and...
Tjalsma, Harold, Bolhuis, Albert, Jongbloed, Jan D. H., Bron, Sierd, Van Dijl, Jan Maarten
One of the most salient features of Bacillus subtilis and related bacilli is their natural capacity to secrete a variety of proteins into their environment, frequently to high concentrations. This...
Tjalsma, Harold, Bolhuis, Albert, Van Roosmalen, Maarten L., Wiegert, Thomas, Schumann, Wolfgang, Broekhuizen, Cees P., ...
Approximately 47% of the genes of the Gram-positive bacterium Bacillus subtilis belong to paralogous gene families. The present studies were aimed at the functional analysis of the sip gene family of...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Smits, Wiep Klaas, Dubois, Jean-Yves F., Bron, Sierd, Van Dijl, Jan Maarten, Kuipers, Oscar P.
Thioredoxins are important thiol-reactive proteins. Most knowledge about this class of proteins is derived from proteome studies, and little is known about the global transcriptional response of...
Dröge, Melloney J., Boersma, Ykelien L., Braun, Peter G., Buining, Robbert Jan, Julsing, Mattijs K., Selles, Karin G. A., ...
Using the phage display technology, a protein can be displayed at the surface of bacteriophages as a fusion to one of the phage coat proteins. Here we describe development of this method for fusion...
Van Dijl, Jan Maarten, Kutejová, Eva, Suda, Kitaru, Perečko, Dušan, Schatz, Gottfried, Suzuki, Carolyn K.
The ATP-dependent Lon protease of Saccharomyces cerevisiae mitochondria is required for selective proteolysis in the matrix, maintenance of mitochondrial DNA, and respiration-dependent growth. Lon...
Evaluation of Bottlenecks in the Late Stages of Protein Secretion in Bacillus subtilis
Bolhuis, Albert, Tjalsma, Harold, Smith, Hilde E., De Jong, Anne, Meima, Rob, Venema, Gerard, ...
Despite a high capacity for secretion of homologous proteins, the secretion of heterologous proteins by Bacillus subtilis is frequently inefficient. In the present studies, we have investigated and...
Tjalsma, Harold, Van Den Dolder, Juliëtte, Meijer, Wilfried J. J., Venema, Gerard, Bron, Sierd, Van Dijl, Jan Maarten
The gram-positive eubacterium Bacillus subtilis is the organism with the largest number of paralogous type I signal peptidases (SPases) known. These are specified both by chromosomal and...
Tjalsma, Harold, Bolhuis, Albert, Jongbloed, Jan D. H., Bron, Sierd, Van Dijl, Jan Maarten
One of the most salient features of Bacillus subtilis and related bacilli is their natural capacity to secrete a variety of proteins into their environment, frequently to high concentrations. This...
Tjalsma, Harold, Bolhuis, Albert, Van Roosmalen, Maarten L., Wiegert, Thomas, Schumann, Wolfgang, Broekhuizen, Cees P., ...
Approximately 47% of the genes of the Gram-positive bacterium Bacillus subtilis belong to paralogous gene families. The present studies were aimed at the functional analysis of the sip gene family of...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Smits, Wiep Klaas, Dubois, Jean-Yves F., Bron, Sierd, Van Dijl, Jan Maarten, Kuipers, Oscar P.
Thioredoxins are important thiol-reactive proteins. Most knowledge about this class of proteins is derived from proteome studies, and little is known about the global transcriptional response of...
Dröge, Melloney J., Boersma, Ykelien L., Braun, Peter G., Buining, Robbert Jan, Julsing, Mattijs K., Selles, Karin G. A., ...
Using the phage display technology, a protein can be displayed at the surface of bacteriophages as a fusion to one of the phage coat proteins. Here we describe development of this method for fusion...
Darmon, Elise , Dorenbos, Ronald, Meens, Jochen, Freudl, Roland, Antelmann, Haike, Hecker, Michael, ...
The gram-positive bacterium Bacillus subtilis secretes high levels of proteins into its environment. Most of these secretory proteins are exported from the cytoplasm in an unfolded state and have to...
Zweers, Jessica C, Barák, Imrich, Becher, Dörte, Driessen, Arnold JM, Hecker, Michael, Kontinen, Vesa P, ...
Sabat, Artur J, Wladyka, Benedykt, Kosowska-Shick, Klaudia, Grundmann, Hajo, Van Dijl, Jan Maarten, Kowal, Julia, ...
Dubois, Jean-Yves F., Freudl, Roland, Quax, Wim J., Van Dijl, Jan Maarten
Disulfide bonds are important for the correct folding, structural integrity, and activity of many biotechnologically relevant proteins. For synthesis and subsequent secretion of these proteins in...
Kolkman, Marc A. B., Van Der Ploeg, René, Bertels, Michael, Van Dijk, Maurits, Van Der Laan, Joop, Van Dijl, Jan Maarten, ...
Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the...
Dubois, Jean-Yves F., Schurich, Anna K. C., Reis, Carlos R., Ensing, Hendrik T., Trip, Erik N., ...
Bacillus subtilis strain 168 produces the extremely stable lantibiotic sublancin 168, which has a broad spectrum of bactericidal activity. Both sublancin 168 production and producer immunity are...
Bacillus subtilis is a well-established cellular factory for proteins and fine chemicals. In particular, the direct secretion of proteinaceous products into the growth medium greatly facilitates...