Jan Schirawski

Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis (2006)

Kahmann, Regine, Bölker, Michael, Ma, Li-Jun, Brefort, Thomas, Saville, Barry J, ...

Ustilago maydis is a ubiquitous pathogen of maize and a well-established model organism for the study of plant-microbe interactions. This basidiomycete fungus does not use aggressive virulence...

Availability of O2 as a Substrate in the Cytoplasm of Bacteria under Aerobic and Microaerobic Conditions

Arras, Tanja, Schirawski, Jan, Unden, Gottfried

The growth rates of Pseudomonas putida KT2442 and mt-2 on benzoate, 4-hydroxybenzoate, or 4-methylbenzoate showed an exponential decrease with decreasing oxygen tensions (partial O2 tension [pO2]...

The fnr Gene of Bacillus licheniformis and the Cysteine Ligands of the C-Terminal FeS Cluster

Klinger, Anette, Schirawski, Jan, Glaser, Philippe, Unden, Gottfried

In the facultatively anaerobic bacterium Bacillus licheniformis a gene encoding a protein of the fumarate nitrate reductase family of transcriptional regulators (Fnr) was isolated. Unlike Fnr...

Identification and Functional Analysis of the Turnip Yellow Mosaic Tymovirus Subgenomic Promoter

Schirawski, Jan, Voyatzakis, Ariane, Zaccomer, Bruno, Bernardi, Françoise, Haenni, Anne-Lise

Most plant viruses rely on the production of subgenomic RNAs (sgRNAs) for the expression of their genes and survival in the plant. Although this is a widely adopted strategy among viruses, the...

Molecular Characterization of Cadmium Resistance in Streptococcus thermophilus Strain 4134: an Example of Lateral Gene Transfer

Schirawski, Jan, Hagens, Werner, Fitzgerald, Gerald F., Van Sinderen, Douwe

Two genes (cadCSt and cadASt [subscript St represents Streptococcus thermophilus]), located on the chromosome of S. thermophilus 4134, were shown to constitute a cadmium/zinc resistance cassette. The...

An unusual MAP kinase is required for efficient penetration of the plant surface by Ustilago maydis

Brachmann, Andreas, Schirawski, Jan, Müller, Philip, Kahmann, Regine

In Ustilago maydis, pathogenic development is controlled by a heterodimer of the two homeodomain proteins bW and bE. We have identified by RNA fingerprinting a b-regulated gene, kpp6, which encodes...

The 5′-Proximal Hairpin of Turnip Yellow Mosaic Virus RNA: Its Role in Translation and Encapsidation

Bink, Hugo H. J., Schirawski, Jan, Haenni, Anne-Lise, Pleij, Cornelis W. A.

The RNA genome of turnip yellow mosaic virus (TYMV) consists of more than 6,000 nucleotides. During a study of the roles of the two hairpins located in its 90-nucleotide 5′ untranslated region, it...

Mating Type Loci of Sporisorium reilianum: Novel Pattern with Three a and Multiple b Specificities

Schirawski, Jan, Heinze, Bernadette, Wagenknecht, Martin, Kahmann, Regine

Sporisorium reilianum and Ustilago maydis are two closely related smut fungi, which both infect maize but differ fundamentally in their mode of plant invasion and site of symptom development. As a...

Endoplasmic Reticulum Glucosidase II Is Required for Pathogenicity of Ustilago maydisW⃞

Schirawski, Jan, Böhnert, Heidi U., Steinberg, Gero, Snetselaar, Karen, Adamikowa, Lubica, Kahmann, Regine

We identified a nonpathogenic strain of Ustilago maydis by tagging mutagenesis. The affected gene, glucosidase1 (gas1), displays similarity to catalytic α-subunits of endoplasmic reticulum (ER)...

Availability of O2 as a Substrate in the Cytoplasm of Bacteria under Aerobic and Microaerobic Conditions

Arras, Tanja, Schirawski, Jan, Unden, Gottfried

The growth rates of Pseudomonas putida KT2442 and mt-2 on benzoate, 4-hydroxybenzoate, or 4-methylbenzoate showed an exponential decrease with decreasing oxygen tensions (partial O2 tension [pO2]...

The fnr Gene of Bacillus licheniformis and the Cysteine Ligands of the C-Terminal FeS Cluster

Klinger, Anette, Schirawski, Jan, Glaser, Philippe, Unden, Gottfried

In the facultatively anaerobic bacterium Bacillus licheniformis a gene encoding a protein of the fumarate nitrate reductase family of transcriptional regulators (Fnr) was isolated. Unlike Fnr...

Identification and Functional Analysis of the Turnip Yellow Mosaic Tymovirus Subgenomic Promoter

Schirawski, Jan, Voyatzakis, Ariane, Zaccomer, Bruno, Bernardi, Françoise, Haenni, Anne-Lise

Most plant viruses rely on the production of subgenomic RNAs (sgRNAs) for the expression of their genes and survival in the plant. Although this is a widely adopted strategy among viruses, the...

Molecular Characterization of Cadmium Resistance in Streptococcus thermophilus Strain 4134: an Example of Lateral Gene Transfer

Schirawski, Jan, Hagens, Werner, Fitzgerald, Gerald F., Van Sinderen, Douwe

Two genes (cadCSt and cadASt [subscript St represents Streptococcus thermophilus]), located on the chromosome of S. thermophilus 4134, were shown to constitute a cadmium/zinc resistance cassette. The...

An unusual MAP kinase is required for efficient penetration of the plant surface by Ustilago maydis

Brachmann, Andreas, Schirawski, Jan, Müller, Philip, Kahmann, Regine

In Ustilago maydis, pathogenic development is controlled by a heterodimer of the two homeodomain proteins bW and bE. We have identified by RNA fingerprinting a b-regulated gene, kpp6, which encodes...

The 5′-Proximal Hairpin of Turnip Yellow Mosaic Virus RNA: Its Role in Translation and Encapsidation

Bink, Hugo H. J., Schirawski, Jan, Haenni, Anne-Lise, Pleij, Cornelis W. A.

The RNA genome of turnip yellow mosaic virus (TYMV) consists of more than 6,000 nucleotides. During a study of the roles of the two hairpins located in its 90-nucleotide 5′ untranslated region, it...

Mating Type Loci of Sporisorium reilianum: Novel Pattern with Three a and Multiple b Specificities

Schirawski, Jan, Heinze, Bernadette, Wagenknecht, Martin, Kahmann, Regine

Sporisorium reilianum and Ustilago maydis are two closely related smut fungi, which both infect maize but differ fundamentally in their mode of plant invasion and site of symptom development. As a...

Endoplasmic Reticulum Glucosidase II Is Required for Pathogenicity of Ustilago maydisW⃞

Schirawski, Jan, Böhnert, Heidi U., Steinberg, Gero, Snetselaar, Karen, Adamikowa, Lubica, Kahmann, Regine

We identified a nonpathogenic strain of Ustilago maydis by tagging mutagenesis. The affected gene, glucosidase1 (gas1), displays similarity to catalytic α-subunits of endoplasmic reticulum (ER)...

A Ferroxidation/Permeation Iron Uptake System Is Required for Virulence in Ustilago maydis[W]

Eichhorn, Heiko, Lessing, Franziska, Winterberg, Britta, Schirawski, Jan, Kämper, Jörg, Müller, Philip, ...

In the smut fungus Ustilago maydis, a tightly regulated cAMP signaling cascade is necessary for pathogenic development. Transcriptome analysis using whole genome microarrays set up to identify...