Zaidi, Sayyed K., Dowdy, Christopher R., Van Wijnen, Andre J., Lian, Jane B., Raza, Azra, Stein, Janet L., ...
Disruption of Runx1/AML1 subnuclear localization, either by a single amino acid substitution or by a chromosomal translocation [e.g., t(8;21)], is linked to the etiology of acute myeloid leukemia...
Survival responses of human embryonic stem cells to DNA damage (2009)
Filion, Tera M., Qiao, Meng, Ghule, Prachi N., Mandeville, Matthew, Van Wijnen, Andre J., Stein, Janet L., ...
Pluripotent human embryonic stem (hES) cells require mechanisms to maintain genomic integrity in response to DNA damage that could compromise competency for lineage-commitment, development, and...
Stein, Gary S., Zaidi, Sayyed K., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., ...
There is growing awareness that the fidelity of gene expression necessitates coordination of transcription factor metabolism and organization of genes and regulatory proteins within the...
Transcription-factor-mediated epigenetic control of cell fate and lineage commitment (2009)
Stein, Gary S., Zaidi, Sayyed K., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., ...
Epigenetic control is required to maintain competency for the activation and suppression of genes during cell division. The association between regulatory proteins and target gene loci during mitosis...
A Runx2 threshold for the cleidocranial dysplasia phenotype (2009)
Lou, Yang, Javed, Amjad, Hussain, Sadiq, Colby, Jennifer, Frederick, Dana, Pratap, Jitesh, ...
Cleidocranial dysplasia (CCD) in humans is an autosomal-dominant skeletal disease that results from mutations in the bone-specific transcription factor RUNX2 (CBFA1/AML3). However, distinct RUNX2...
Ricardo Medina, Angela Miele-chamberl, Rong-lin Xie, Janet L. Stein, ...
HiNF-P and its cofactor p220 NPAT are principal factors regulating histone gene expression at the G1-S phase cell cycle transition. Here,we have investigated whether HiNF-P controls other cell cycle...
Pande, Sandhya, Ali, Syed A., Dowdy, Christopher, Zaidi, Sayyed K., Ito, Kosei, Ito, Yoshiaki, ...
Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation...
Bakshi, Rachit, Zaidi, Sayyed K., Pande, Sandhya, Hassan, Mohammad Q., Young, Daniel W., Montecino, Martin A., ...
RUNX1/AML1 is required for definitive hematopoiesis and is frequently targeted by chromosomal translocations in acute myeloid leukemia (AML). The t(8;21)-related AML1-ETO fusion protein blocks...
Bakshi, Rachit, Zaidi, Sayyed K., Pande, Sandhya, Hassan, Mohammad Q., Young, Daniel W., Montecino, Martin A., ...
RUNX1/AML1 is required for definitive hematopoiesis and is frequently targeted by chromosomal translocations in acute myeloid leukemia (AML). The t(8;21)-related AML1-ETO fusion protein blocks...
Ghule, Prachi N., Dominski, Zbigniew, Yang, Xiao-Cui, Marzluff, William F., Becker, Klaus A., Harper, J. Wade, ...
Human embryonic stem (hES) cells have an abbreviated G(1) phase of the cell cycle. How cells expedite G(1) events that are required for the initiation of S phase has not been resolved. One key...
Medina, Ricardo F., Buck, Timothy F., Zaidi, Sayyed K., Miele, Angela, Lian, Jane B., Stein, Janet L., ...
Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of...
Medina, Ricardo F., Buck, Timothy F., Zaidi, Sayyed K., Miele, Angela, Lian, Jane B., Stein, Janet L., ...
Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of...
Pratap, Jitesh, Wixted, John J., Gaur, Tripti, Zaidi, Sayyed K., Dobson, Jason, Gokul, Karthiga Devi, ...
Runx2, required for bone formation, is ectopically expressed in breast cancer cells. To address the mechanism by which Runx2 contributes to the osteolytic disease induced by MDA-MB-231 cells, we...
Pratap, Jitesh, Wixted, John J., Gaur, Tripti, Zaidi, Sayyed K., Dobson, Jason, Gokul, Karthiga Devi, ...
Runx2, required for bone formation, is ectopically expressed in breast cancer cells. To address the mechanism by which Runx2 contributes to the osteolytic disease induced by MDA-MB-231 cells, we...
A microRNA signature for a BMP2-induced osteoblast lineage commitment program (2008)
Li, Zhaoyong, Hassan, Mohammad Q., Volinia, Stefano, Van Wijnen, Andre J., Stein, Janet L., Croce, Carlo M., ...
Bone morphogenetic proteins (BMPs) are potent morphogens that activate transcriptional programs for lineage determination. How BMP induction of a phenotype is coordinated with microRNAs (miRNAs) that...
Javed, Amjad, Afzal, Faiza, Bae, Jong-Sup, Gutierrez, Soraya E., Zaidi, Kaleem, Pratap, Jitesh, ...
BMP2 signaling and RUNX2 regulatory pathways converge for transcriptional control of bone formation in vivo. SMAD proteins are recruited to RUNX2 regulatory complexes via an overlapping nuclear...
Hassan, Mohammad Q., Saini, Sharanjot, Gordon, Jonathan A. R., Van Wijnen, Andre J., Montecino, Martin A., Stein, Janet L., ...
The osteoinductive BMP2 signal facilitates commitment to the osteoblast phenotype by inducing several classes of early response genes. Among these are bone-related HOX factors, homeodomain, RUNX and...
Zhang, Ying, Hassan, Mohammad Q., Li, Zhaoyong, Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., ...
Helix-loop-helix (HLH) transcription factors are key regulators of neurogenesis, myogenesis and osteogenesis. Here the relative contributions of multiple classes of HLH factors to the expression of...
Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain (2008)
Jeong, Jae-Hwan, Jin, Jung-Sook, Kim, Hyun-Nam, Kang, Sang-Min, Liu, Julie C., Lengner, Christopher J., ...
Runx2 is a master transcription factor for chondrocyte and osteoblast differentiation and bone formation. However expression of Runx2 (by RT-PCR), has been reported in non-skeletal tissues such as...
Teplyuk, Nadiya M., Galindo, Mario, Teplyuk, Viktor, Pratap, Jitesh, Young, Daniel W., Lapointe, David S., ...
Runt-related transcription factor 2 (Runx2) controls lineage commitment, proliferation, and anabolic functions of osteoblasts as the subnuclear effector of multiple signaling axes (e.g. transforming...
Stein, Gary S., Zaidi, Sayyed K., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., ...
The regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex...
Van Der Deen, Margaretha, Hassan, Mohammad Q., Pratap, Jitesh, Teplyuk, Nadiya M., Young, Daniel W., Javed, Amjad, ...
Normal cell growth and differentiation of bone cells requires the sequential expression of cell type specific genes to permit lineage specification and development of cellular phenotypes....
In situ nuclear organization of regulatory machinery (2008)
Pockwinse, Shirwin M., Zaidi, Sayyed K., Medina, Ricardo F., Bakshi, Rachit, Kota, Krishna P., Ali, Syed A., ...
Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of...
Phenotypic transcription factors epigenetically mediate cell growth control (2008)
Ali, Syed A., Zaidi, Sayyed K., Dacwag, Caroline S., Salma, Nunciada, Young, Daniel W., Shakoori, A. Rauf, ...
Ribosomal RNA (rRNA) genes are down-regulated during osteogenesis, myogenesis, and adipogenesis, necessitating a mechanistic understanding of interrelationships between growth control and phenotype...
Phenotypic transcription factors epigenetically mediate cell growth control (2008)
Ali, Syed A., Zaidi, Sayyed K., Dacwag, Caroline S., Salma, Nunciada, Young, Daniel W., Shakoori, A. Rauf, ...
Ribosomal RNA (rRNA) genes are down-regulated during osteogenesis, myogenesis, and adipogenesis, necessitating a mechanistic understanding of interrelationships between growth control and phenotype...
MicroRNAs 221 and 222 bypass quiescence and compromise cell survival (2008)
Medina, Ricardo F., Zaidi, Sayyed K., Liu, Chang-Gong, Stein, Janet L., Van Wijnen, Andre J., Croce, Carlo M., ...
MicroRNAs (miRNA) have tumor suppressive and oncogenic potential in human cancer, but whether and how miRNAs control cell cycle progression is not understood. To address this question, we carried out...
MicroRNAs 221 and 222 bypass quiescence and compromise cell survival (2008)
Medina, Ricardo F., Zaidi, Sayyed K., Liu, Chang-Gong, Stein, Janet L., Van Wijnen, Andre J., Croce, Carlo M., ...
MicroRNAs (miRNA) have tumor suppressive and oncogenic potential in human cancer, but whether and how miRNAs control cell cycle progression is not understood. To address this question, we carried out...
Montecino, Martin A., Stein, Gary S., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Carvallo, Loreto, ...
Vitamin D is a principal modulator of skeletal gene expression, thus necessitating an understanding of interfaces between the activity of this steroid hormone and regulatory cascades that are...
Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal (2008)
Javed, Amjad, Bae, Jong-Sup, Afzal, Faiza, Gutierrez, Soraya E., Pratap, Jitesh, Zaidi, Sayyed K., ...
Two regulatory pathways, bone morphogenetic protein (BMP)/transforming growth factor-beta (TGFbeta) and the transcription factor RUNX2, are required for bone formation in vivo. Here we show the...
Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain (2008)
Jeong, Jae-Hwan, Jin, Jung-Sook, Kim, Hyun-Nam, Kang, Sang-Min, Liu, Julie C., Lengner, Christopher J., ...
Runx2 is a master transcription factor for chondrocyte and osteoblast differentiation and bone formation. However expression of Runx2 (by RT-PCR), has been reported in non-skeletal tissues such as...
Zaidi, Sayyed K., Pande, Sandhya, Pratap, Jitesh, Gaur, Tripti, Grigoriu, Simina R., Ali, Syed A., ...
The osteogenic Runt-related (Runx2) transcription factor negatively regulates proliferation and ribosomal gene expression in normal diploid osteoblasts, but is up-regulated in metastatic breast and...
Zaidi, Sayyed K., Pande, Sandhya, Pratap, Jitesh, Gaur, Tripti, Grigoriu, Simina R., Ali, Syed A., ...
The osteogenic Runt-related (Runx2) transcription factor negatively regulates proliferation and ribosomal gene expression in normal diploid osteoblasts, but is up-regulated in metastatic breast and...
Xie, Ronglin, Liu, Lijun, Mitra, Partha, Stein, Janet L., Van Wijnen, Andre J., Stein, Gary S.
Histone nuclear factor P (HiNF-P) activates histone H4 gene transcription at the G1/S phase transition upon association with its cyclin E/CDK2 responsive co-factor p220NPAT. Here we characterize the...
Carvallo, Loreto, Henriquez, Berta, Paredes, Roberto, Olate, Juan, Onate, Sergio, Van Wijnen, Andre J., ...
Binding of 1alpha,25-dihydroxy vitamin D(3) to the C-terminal ligand-binding domain (LBD) of its receptor (VDR) induces a conformational change that enables interaction of VDR with transcriptional...
Montecino, Martin A., Stein, Janet L., Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Cruzat, Fernando, ...
Chromatin organization within the nuclear compartment is a fundamental mechanism to regulate the expression of eukaryotic genes. During the last decade, a number of nuclear protein complexes with the...
Miele, Angela, Medina, Ricardo F., Van Wijnen, Andre J., Stein, Gary S., Stein, Janet L.
HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4...
Miele, Angela, Medina, Ricardo F., Van Wijnen, Andre J., Stein, Gary S., Stein, Janet L.
HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4...
Nuclear microenvironments in biological control and cancer (2007)
Zaidi, Sayyed K., Young, Daniel W., Javed, Amjad, Pratap, Jitesh, Montecino, Martin A., Van Wijnen, Andre J., ...
Nucleic acids and regulatory proteins are compartmentalized in microenvironments within the nucleus. This subnuclear organization may support convergence and the integration of physiological signals...
Ghule, Prachi N., Becker, Klaus A., Harper, J. Wade, Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., ...
Human embryonic stem (ES) cells have an expedited cell cycle ( approximately 15 h) due to an abbreviated G1 phase ( approximately 2.5 h) relative to somatic cells. One principal regulatory event...
Arriagada, Gloria, Paredes, Roberto, Olate, Juan, Van Wijnen, Andre J., Lian, Jane B., Stein, Gary S., ...
Upon ligand binding the 1alpha,25-dihydroxy Vitamin D3 receptor (VDR) undergoes a conformational change that allows interaction with coactivator proteins including p160/SRC family members and the...
Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Javed, Amjad, Montecino, Martin A., ...
Young, Daniel W., Hassan, Mohammad Q., Yang, Xiaoqing, Galindo, Mario, Javed, Amjad, Zaidi, Sayyed K., ...
During cell division, cessation of transcription is coupled with mitotic chromosome condensation. A fundamental biological question is how gene expression patterns are retained during mitosis to...
Hassan, Mohammad Q., Tare, Rahul S., Lee, Suk Hee, Mandeville, Matthew, Weiner, Brian, Montecino, Martin A., ...
HOXA10 is necessary for embryonic patterning of skeletal elements, but its function in bone formation beyond this early developmental stage is unknown. Here we show that HOXA10 contributes to...
Hassan, Mohammad Q., Tare, Rahul S., Lee, Suk Hee, Mandeville, Matthew, Weiner, Brian, Montecino, Martin A., ...
HOXA10 is necessary for embryonic patterning of skeletal elements, but its function in bone formation beyond this early developmental stage is unknown. Here we show that HOXA10 contributes to...
An architectural perspective of vitamin D responsiveness (2007)
Montecino, Martin A., Stein, Gary S., Cruzat, Fernando, Marcellini, Sylvain, Stein, Janet L., Lian, Jane B., ...
Vitamin D serves as a principal modulator of skeletal gene transcription, thus necessitating an understanding of interfaces between the activity of this steroid hormone and regulatory cascades that...
Gutierrez, Jose L., Paredes, Roberto, Cruzat, Fernando, Hill, David A., Van Wijnen, Andre J., Lian, Jane B., ...
Changes in local chromatin structure accompany transcriptional activation of eukaryotic genes. In vivo these changes in chromatin organization can be catalyzed by ATP-dependent chromatin-remodeling...
Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2 (2007)
Young, Daniel W., Hassan, Mohammad Q., Pratap, Jitesh, Galindo, Mario, Zaidi, Sayyed K., Lee, Suk Hee, ...
Regulation of ribosomal RNA genes is a fundamental process that supports the growth of cells and is tightly coupled with cell differentiation. Although rRNA transcriptional control by RNA polymerase...
Carvallo, Loreto, Henriquez, Berta, Olate, Juan, Van Wijnen, Andre J., Lian, Jane B., Stein, Gary S., ...
Binding of 1alpha,25-dihydroxy Vitamin D3 to the C-terminal domain (LBD) of its receptor (VDR), induces a conformational change that enables interaction of VDR with transcriptional coactivators such...
Rajgopal, Arun, Young, Daniel W., Mujeeb, Khwaja A., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., ...
Skeletal development and osteoblast maturation require the phenotype promoting activity of the transcription factor RUNX2, which controls both cell growth and differentiation in osteoblasts. We have...
Regulatory roles of Runx2 in metastatic tumor and cancer cell interactions with bone (2006)
Pratap, Jitesh, Lian, Jane B., Javed, Amjad, Barnes, George L., Van Wijnen, Andre J., Stein, Janet L., ...
The three mammalian Runt homology domain transcription factors (Runx1, Runx2, Runx3) support biological control by functioning as master regulatory genes for the differentiation of distinct tissues....
HiNF-P is a bifunctional regulator of cell cycle controlled histone H4 gene transcription (2006)
Mitra, Partha, Xie, Ronglin, Harper, J. Wade, Stein, Janet L., Stein, Gary S., Van Wijnen, Andre J.
Cell cycle progression beyond the G1/S phase transition requires the activation of a transcription complex containing histone nuclear factor P (HiNF-P) and nuclear protein mapped to ataxia...
Becker, Klaus A., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Stein, Gary S.
Rapid self-renewal of human embryonic stem (ES) cells (NIH designation WA01 and WA09) is accommodated by an abbreviated cell cycle due to a reduction in the G1 phase. Thus, molecular mechanisms...
Hassan, Mohammad Q., Tare, Rahul S., Lee, Suk Hee, Mandeville, Matthew, Morasso, Maria I., Javed, Amjad, ...
Several homeodomain (HD) proteins are critical for skeletal patterning and respond directly to BMP2 as an early step in bone formation. RUNX2, the earliest transcription factor proven essential for...
Networks and hubs for the transcriptional control of osteoblastogenesis (2006)
Lian, Jane B., Stein, Gary S., Javed, Amjad, Van Wijnen, Andre J., Stein, Janet L., Montecino, Martin, ...
We present an overview of the concepts of tissue-specific transcriptional control mechanisms essential for development of the bone cell phenotype. BMP2 induced transcription factors constitute a...
An architectural perspective of cell-cycle control at the G1/S phase cell-cycle transition (2006)
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Montecino, Martin A., Zaidi, Sayyed K., ...
A prominent role for the execution of cell cycle and growth regulatory mechanisms within the three-dimensional context of nuclear architecture is becoming increasingly evident. Signaling pathways and...
Self-renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase (2006)
Becker, Klaus A., Ghule, Prachi N., Therrien, Jaclyn A., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., ...
Competency for self-renewal of human embryonic stem (ES) cells is linked to pluripotency. However, there is a critical paucity of fundamental parameters of human ES cell division. In this study we...
Alterations in intranuclear localization of Runx2 affect biological activity (2006)
Zaidi, Sayyed K., Javed, Amjad, Pratap, Jitesh, Schroeder, Tania M., Westendorf, Jennifer J., Lian, Jane B., ...
The transcription factor Runx2 controls osteoblast proliferation and differentiation. Runx2 organizes and assembles gene-regulatory complexes in nuclear microenvironments where target genes are...
Bravo, Soraya, Paredes, Roberto, Izaurieta, Pamela, Lian, Jane B., Stein, Janet L., Stein, Gary S., ...
1alpha,25-dihydroxy vitamin D3 has a major role in the regulation of the bone metabolism as it promotes the expression of key bone-related proteins in osteoblastic cells. In recent years it has...
Bae, Jong-Sup, Gutierrez, Soraya E., Narla, Radhika, Pratap, Jitesh, Devados, Rajitha, Van Wijnen, Andre J., ...
The Runx2/Cbfa1 transcription factor is a scaffolding protein that promotes osteoblast differentiation; however, the specific Runx2-functional domains required for induction of the osteogenic lineage...
Villagra, Alejandro, Cruzat, Fernando, Carvallo, Loreto, Paredes, Roberto, Olate, Juan, Van Wijnen, Andre J., ...
Tissue-specific activation of the osteocalcin (OC) gene is associated with changes in chromatin structure at the promoter region. Two nuclease-hypersensitive sites span the key regulatory elements...
Gaur, Tripti, Rich, Lillian, Lengner, Christopher J., Hussain, Sadiq, Trevant, Brune, Ayers, David, ...
Canonical Wnt signaling (beta-catenin/TCF) has emerged as a key regulator of skeletogenesis. In this study, chondrogenesis is examined in a mouse model in which the Wnt antagonist secreted frizzled...
Kim, Yeon-Ju, Kim, Hyun-Nam, Park, Eui-Kyun, Lee, Byung-Heon, Ryoo, Hyun-Mo, Kim, Shin-Yoon, ...
Osterix is a bone-related transcription factor that functions genetically downstream of Runx2, which controls both growth and differentiation in osteoblasts. Here we assessed the biological function...
Kim, Hyo-Jin, Nam, Soon-Hyeun, Kim, Hyun-Jung, Park, Hyo-Sang, Ryoo, Hyun-Mo, Kim, Shin-Yoon, ...
Cleidocranial dysplasia (CCD) is an autosomal dominant disorder caused by haploinsufficiency of the RUNX2 gene. In this study, we analyzed by direct sequencing RUNX2 mutations from eleven CCD...
Pratap, Jitesh, Javed, Amjad, Languino, Lucia R., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., ...
The Runx2 (Cbfa1/AML3) transcription factor and matrix metalloproteinase 9 (MMP9) are key regulators of growth plate maturation and bone formation. The genes for both proteins are characteristic...
Li, Xiangen, Vradii, Diana, Gutierrez, Soraya E.u, Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., ...
Many types of acute myelogenous leukemia involve chromosomal translocations that target the C-terminus of Runx1/AML1 transcription factor, a master regulator of hematopoiesis. The C-terminus of...
Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Javed, Amjad, Montecino, Martin A., ...
The architecturally associated subnuclear organization of nucleic acids and cognate regulatory factors suggests functional interrelationships between nuclear structure and gene expression. Mechanisms...
Holmes, William F., Braastad, Corey D., Mitra, Partha, Hampe, Cornelia, Doenecke, Detlef, Albig, Werner, ...
The replication of eukaryotic genomes necessitates the coordination of histone biosynthesis with DNA replication at the onset of S phase. The multiple histone H4 genes encode identical proteins, but...
Microtubule-dependent nuclear-cytoplasmic shuttling of Runx2 (2005)
Pockwinse, Shirwin M., Rajgopal, Arun, Young, Daniel W., Mujeeb, Khwaja A., Nickerson, Jeffrey A., Javed, Amjad, ...
RUNX/AML transcription factors are critical regulators of cell growth and differentiation in multiple lineages and have been linked to human cancers including acute myelogenous leukemia (RUNX1), as...
Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression (2005)
Gaur, Tripti, Lengner, Christopher J., Hovhannisyan, Hayk, Bhat, Ramesh A., Bodine, Peter V. N., Komm, Barry S., ...
Both activating and null mutations of proteins required for canonical WNT signaling have revealed the importance of this pathway for normal skeletal development. However, tissue-specific...
Miele, Angela, Braastad, Corey D., Holmes, William F., Mitra, Partha, Medina, Ricardo F., Xie, Ronglin, ...
Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the...
Vradii, Diana, Wagner, Stefan, Doan, Diem N., Nickerson, Jeffrey A., Montecino, Martin A., Lian, Jane B., ...
Many mammalian SWI/SNF complexes use Brahma-related gene 1 (Brg1) as a catalytic subunit to remodel nucleosomes for transcription regulation. In several mesenchymal cells and tissues, expression of a...
Vradii, Diana, Zaidi, Sayyed K., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
The multifunctional C terminus of the hematopoietic AML1 transcription factor interacts with coregulatory proteins, supports the convergence and integration of physiological signals, and contains the...
Saltman, Laura H., Javed, Amjad, Ribadeneyra, John, Hussain, Sadiq, Young, Daniel W., Osdoby, Philip, ...
The osteoclast is a highly polarized multinucleated cell that resorbs bone. Using high resolution immunofluorescence microscopy, we demonstrated that all nuclei of an osteoclast are transcriptionally...
Galindo, Mario, Pratap, Jitesh, Young, Daniel W., Hovhannisyan, Hayk, Im, Hee-Jeong, Choi, Je-Yong, ...
The Runx2 (CBFA1/AML3/PEBP2alphaA) transcription factor promotes skeletal cell differentiation, but it also has a novel cell growth regulatory activity in osteoblasts. We addressed here whether Runx2...
Nkx3.2-mediated repression of Runx2 promotes chondrogenic differentiation (2005)
Lengner, Christopher J., Hassan, Mohammad Q., Serra, Ryan W., Lepper, Christoph, Van Wijnen, Andre J., Stein, Janet L., ...
Runx2, a transcription factor known to be essential for osteoblast maturation and skeletogenesis, is also expressed in pre-cartilaginous mesenchymal condensations in the developing embryo. It is...
The dynamic organization of gene-regulatory machinery in nuclear microenvironments (2005)
Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin A., ...
Nuclear components are functionally linked with the dynamic temporal and spatial compartmentalization, sorting and integration of regulatory information to facilitate its selective use. For example,...
Javed, Amjad, Barnes, George L., Pratap, Jitesh, Antkowiak, Tomasz, Gerstenfeld, Louis C., Van Wijnen, Andre J., ...
Runx transcription factors comprise a family of proteins that are essential for organogenesis. A unique nuclear matrix-targeting signal in the C terminus directs these factors to their appropriate...
Afzal, Faiza, Pratap, Jitesh, Ito, Kosei, Ito, Yoshiaki, Stein, Janet L., Van Wijnen, Andre J., ...
The coordinated activity of Runx2 and BMP/TGFbeta-activated Smads is critical for formation of the skeleton, but the precise structural basis for the Runx2/Smad interaction has not been resolved. By...
Young, Daniel W., Pratap, Jitesh, Javed, Amjad, Weiner, Brian, Yasuyuki, Ohkawa, Van Wijnen, Andre J., ...
Development of bone tissue requires maturation of osteoblasts from mesenchymal precursors. BMP2, a member of the TGFbeta superfamily, and the Runx2 (AML3/Cbfa1) transcription factor, a downstream...
Functional characterization of a human histone gene cluster duplication (2004)
Braastad, Corey D., Hovhannisyan, Hayk, Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
Histones are the major protein component of nucleosomes, and de novo histone synthesis is essential for packaging newly replicated DNA into chromatin. As a result, histone gene expression is...
Hassan, Mohammad Q., Javed, Amjad, Morasso, Maria I., Karlin, Jeremy, Montecino, Martin A., Van Wijnen, Andre J., ...
Genetic studies show that Msx2 and Dlx5 homeodomain (HD) proteins support skeletal development, but null mutation of the closely related Dlx3 gene results in early embryonic lethality. Here we find...
Paredes, Roberto, Arriagada, Gloria, Cruzat, Fernando, Villagra, Alejandro, Olate, Juan, Zaidi, Kaleem, ...
Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1alpha,25-dihydroxyvitamin D3 via its...
Smith, Nathan, Dong, Yufeng, Lian, Jane B., Pratap, Jitesh, Kingsley, Paul D., Van Wijnen, Andre J., ...
Identifying the genetic pathways that regulate skeletal development is necessary to correct a variety of cartilage and bone abnormalities. The Runx family of transcription factors play a fundamental...
Young, Daniel W., Zaidi, Sayyed K., Furcinitti, Paul S., Javed, Amjad, Van Wijnen, Andre J., Stein, Janet L., ...
Regulatory machinery for replication and gene expression is punctately organized in supramolecular complexes that are compartmentalized in nuclear microenvironments. Quantitative approaches are...
Gutierrez, Soraya E., Liu, Jilin, Javed, Amjad, Montecino, Martin A., Stein, Gary S., Lian, Jane B., ...
Vitamin D receptor (VDR) and Runx2 are key regulators of tissue-specific gene transcription. Using the bone-related osteocalcin (OC) gene, we have previously shown that Runx2 is required for the...
Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin A., ...
The molecular logistics of nuclear regulatory processes necessitate temporal and spatial regulation of protein-protein and protein-DNA interactions in response to physiological cues. Biochemical, in...
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
Chromatin immunoprecipitation (2004)
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
In situ immunofluorescence analysis: immunofluorescence microscopy (2004)
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
Analysis of in vivo gene expression using epitope-tagged proteins (2004)
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
Protein-deoxyribonucleic acid interactions linked to gene expression: DNase I digestion (2004)
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
Immunofluorescence analysis using epitope-tagged proteins: in vitro system (2004)
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...
Primary mouse embryonic fibroblasts: a model of mesenchymal cartilage formation (2004)
Lengner, Christopher J., Lepper, Christoph, Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.
Cartilage formation is an intricate process that requires temporal and spatial organization of regulatory factors in order for a mesenchymal progenitor cell to differentiate through the distinct...
Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Montecino, Martin A., Javed, Amjad, ...
We present an overview of Runx involvement in regulatory mechanisms that are requisite for fidelity of bone cell growth and differentiation, as well as for skeletal homeostasis and the structural and...
Stein, Gary S., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., Javed, Amjad, ...
Functional interrelationships between the intranuclear organization of nucleic acids and regulatory proteins are obligatory for fidelity of transcriptional activation and repression. In this article,...
Regulatory controls for osteoblast growth and differentiation: role of Runx/Cbfa/AML factors (2004)
Lian, Jane B., Javed, Amjad, Zaidi, Sayyed K., Lengner, Christopher J., Montecino, Martin, Van Wijnen, Andre J., ...
Formation of skeletal elements during embryogenesis and the dynamic remodeling of bone in the adult involve an exquisite interplay of developmental cues, signaling proteins, transcription factors,...
Zaidi, Sayyed K., Sullivan, Andrew J., Medina, Ricardo F., Ito, Yoshiaki, Van Wijnen, Andre J., Stein, Janet L., ...
Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of...
Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Javed, Amjad, Montecino, Martin A., ...
The temporal and spatial organization of transcriptional regulatory machinery provides microenvironments within the nucleus where threshold concentrations of genes and cognate factors facilitate...
Zaidi, Sayyed K., Young, Daniel W., Pockwinse, Shirwin M., Javed, Amjad, Lian, Jane B., Stein, Janet L., ...
Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately...
Kim, Joung-Ok, Kim, Hyun-Nam, Hwang, Mi-Hye, Shin, Hong-In, Kim, Shin-Yoon, Park, Rang-Woon, ...
Recent advances in laser microdissection allow for precise removal of pure cell populations from morphologically preserved tissue sections. However, RNA from paraffin-embedded samples is usually...
Mitra, Partha, Xie, Ronglin, Medina, Ricardo F., Hovhannisyan, Hayk, Zaidi, Sayyed K., Wei, Yue, ...
At the G(1)/S phase cell cycle transition, multiple histone genes are expressed to ensure that newly synthesized DNA is immediately packaged as chromatin. Here we have purified and functionally...
Stein, Gary S., Zaidi, Sayyed K., Braastad, Corey D., Montecino, Martin A., Van Wijnen, Andre J., Choi, Je-Yong, ...
The organization and sorting of regulatory information for transcription, replication and repair depends on components of nuclear architecture. It is necessary, therefore, to understand cellular...
Cell growth regulatory role of Runx2 during proliferative expansion of preosteoblasts (2003)
Pratap, Jitesh, Galindo, Mario, Zaidi, Sayyed K., Vradii, Diana, Bhat, Bheem M., Robinson, John A., ...
The Runx2 (CBFA1/AML3/PEBP2alphaA) transcription factor promotes lineage commitment and differentiation by activating bone phenotypic genes in postproliferative osteoblasts. However, the presence of...
Nuclear microenvironments support physiological control of gene expression (2003)
Stein, Gary S., Lian, Jane B., Montecino, Martin A., Stein, Janet L., Van Wijnen, Andre J., Javed, Amjad, ...
There is growing recognition that the organization of nucleic acids and regulatory proteins is functionally linked to the assembly, localization and activity of gene regulatory machinery. Cellular,...
Lian, Jane B., Stein, Janet L., Stein, Gary S., Van Wijnen, Andre J., Montecino, Martin, Javed, Amjad, ...
Development of the osteoblast phenotype requires transcriptional mechanisms that regulate induction of a program of temporally expressed genes. Key components of gene activation, repression, and...
Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Choi, Je-Yong, Pratap, Jitesh, ...
Key components of the basal transcription machinery and several tissue-specific transcription factor complexes are functionally compartmentalized as specialized subnuclear domains. We have identified...
Gupta, Sunita, Luong, Mai X., Bleuming, Sylvia A., Miele, Angela, Luong, Michael, Young, Daniel W., ...
The CCAAT displacement protein (CDP-cut/CUTL1/cux) performs a key proliferation-related function as the DNA binding subunit of the cell cycle controlled HiNF-D complex. HiNF-D interacts with all five...
Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo (2003)
Lian, Jane B., Balint, Eva, Javed, Amjad, Drissi, Hicham, Vitti, Regan, Quinlan, Edward J., ...
The requirement of Runx2 (Cbfal/AML3), a runt homology domain transcription factor essential for bone formation and osteoblast differentiation, is well established. Although Runx2 is expressed in the...
Balint, Eva, Lapointe, David, Drissi, Hicham, Van Der Meijden, Caroline, Young, Daniel W, Van Wijnen, Andre J, ...
Understanding physiological control of osteoblast differentiation necessitates characterization of the regulatory signals that initiate the events directing a cell to lineage commitment and...
Xie, Ronglin, Gupta, Sunita, Miele, Angela, Shiffman, Dov, Stein, Janet L., Stein, Gary S., ...
Cell growth control by interferons (IFNs) involves up-regulation of the tumor suppressor interferon regulatory factor 1 (IRF1). To exert its anti-proliferative effects, this factor must ultimately...
Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Montecino, Martin A., Pratap, Jitesh, ...
RUNX (AML/CBFA/PEBP2) transcription factors serve as paradigms for obligatory relationships between nuclear structure and physiological control of phenotypic gene expression. The RUNX proteins...
Balint, Eva, Lapointe, David S., Drissi, Hicham, Young, Daniel W., Van Wijnen, Andre J., ...
Understanding physiological control of osteoblast differentiation necessitates characterization of the regulatory signals that initiate the events directing a cell to lineage commitment and...
Sierra, Jose, Villagra, Alejandro, Paredes, Roberto, Cruzat, Fernando, Gutierrez, Soraya E., Javed, Amjad, ...
p300 is a multifunctional transcriptional coactivator that serves as an adapter for several transcription factors including nuclear steroid hormone receptors. p300 possesses an intrinsic histone...
Hovhannisyan, Hayk, Cho, Brian C., Mitra, Partha, Montecino, Martin A., Stein, Gary S., Van Wijnen, Andre J., ...
During the shutdown of proliferation and onset of differentiation of HL-60 promyelocytic leukemia cells, expression of the cell cycle-dependent histone genes is downregulated at the level of...
Shen, Jiali, Hovhannisyan, Hayk, Lian, Jane B., Montecino, Martin A., Stein, Gary S., Stein, Janet L., ...
The remodeling of chromatin is required for tissue-specific gene activation to permit interactions of transcription factors and coregulators with their cognate elements. Here, we investigate the...
Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Montecino, Martin A., Javed, Amjad, ...
The architecturally associated subnuclear organization of nucleic acids and cognate regulatory factors suggest functional interrelationships between nuclear structure and gene expression. Mechanisms...
Barseguian, Karina, Lutterbach, Bart, Hiebert, Scott W., Nickerson, Jeffrey A., Lian, Jane B., Stein, Janet L., ...
Leukemic disease can be linked to aberrant gene expression. This often is the result of molecular alterations in transcription factors that lead to their misrouting within the nucleus. The acute...
Harrington, Kimberly Stacy, Javed, Amjad, Drissi, Hicham, McNeil, Sandra Marie, Lian, Jane B., Stein, Janet L., ...
The runt-related transcription factors (RUNX/Cbfa/AML) are essential for cellular differentiation and fetal development. C-terminal truncations of RUNX factors that eliminate the targeting of these...
Lengner, Christopher J., Drissi, Hicham, Choi, Je-Yong, Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., ...
The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo...
Drissi, Hicham, Pouliot, Arlyssa, Stein, Janet L., Van Wijnen, Andre J., Stein, Gary S., Lian, Jane B.
The runt homology transcription factor Runx2/Cbfa1 is essential for bone development and osteoblast differentiation. Regulatory mechanisms that govern Runx2 transcription in osteoblasts define the...
Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites (2002)
Zaidi, Sayyed K., Sullivan, Andrew J., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.
Runx factors control lineage commitment and are transcriptional effectors of Smad signaling. Genetic defects in these pathways interfere with normal development. The in situ localization of Runx and...
Lapointe, David S., Luong, Mai X., Peric-Hupkes, Daniel, Cho, Brian C., Stein, Janet L., ...
The ordered expression of genes after growth factor stimulation in G(1) supportsthe onset of DNA replication. To characterize regulatory events during S-phase when cell cycle progression has become...
Xie, Ronglin, Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
The IFN regulatory factor-2 (IRF-2) oncoprotein controls the cell cycle-dependent expression of histone H4 genes during S phase and may function as a component of an E2F-independent mechanism to...
1,25-(OH)2-vitamin D3 suppresses the bone-related Runx2/Cbfa1 gene promoter (2002)
Drissi, Hicham, Pouliot, Arlyssa, Koolloos, Christian, Stein, Janet L., Lian, Jane B., Stein, Gary S., ...
The steroid hormone 1,25-(OH)2-vitamin D3 (VD3) regulates osteoblast differentiation by either activating or repressing transcription of numerous bone phenotypic genes. We addressed whether VD3 also...
Shen, Jiali, Montecino, Martin A., Lian, Jane B., Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L.
The accessibility of regulatory elements in chromatin represents a principal rate-limiting parameter of gene transcription and is modulated by enzymatic transcriptional co-factors that alter the...
Luong, Mai X., Xing, DongXia, Hesselton, RuthAnn M., Monuki, Edwin S., Jones, Stephen N., ...
Murine CDP/Cux, a homologue of the Drosophila Cut homeoprotein, modulates the promoter activity of cell cycle-related and cell-type-specific genes. CDP/Cux interacts with histone gene promoters as...
Gutierrez, Soraya E., Javed, Amjad, Tennant, Daniel K., Van Rees, Monique, Montecino, Martin A., Stein, Gary S., ...
CCAAT/enhancer-binding proteins (C/EBP) are critical determinants for cellular differentiation and cell type-specific gene expression. Their functional roles in osteoblast development have not been...
Zaidi, Sayyed K., Javed, Amjad, Choi, Je-Yong, Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., ...
Key components of DNA replication and the basal transcriptional machinery as well as several tissue-specific transcription factors are compartmentalized in specialized nuclear domains. In the present...
Mitra, Prasenjit, Vaughan, Patricia S., Stein, Janet L., Stein, Gary S., Van Wijnen, Andre J.
Regulation of histone gene transcription at the G1/S phase transition via the Site II cell cycle control element is distinct from E2F-dependent mechanisms operative at the growth factor-related...
Banerjee, Chaitali, Javed, Amjad, Choi, Je-Yong, Green, Jack, Rosen, Vicki, Van Wijnen, Andre J., ...
Cbfa1/Runx2 is a transcription factor essential for bone formation and osteoblast differentiation. Two major N-terminal isoforms of Cbfa1, designated type I/p56 (PEBP2aA1, starting with the sequence...
Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Zaidi, Sayyed K., Xing, Lianping, Balint, Eva, ...
Runx (Cbfa/AML) transcription factors are critical for tissue-specific gene expression. A unique targeting signal in the C terminus directs Runx factors to discrete foci within the nucleus. Using...
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Montecino, Martin A., Zaidi, Kaleem, ...
The regulated and regulatory components that interrelate nuclear structure and function must be experimentally established. A formidable challenge is to define further the control of transcription...
Javed, Amjad, Barnes, George L., Jasanya, B. O., Stein, Janet L., Gerstenfeld, Louis C., Lian, Jane B., ...
Expression of the bone sialoprotein (BSP) gene, a marker of bone formation, is largely restricted to cells in mineralized tissues. Recent studies have shown that the Cbfa1 (also known as Runx2,...
Xie, Ronglin, Van Wijnen, Andre J., Luong, Mai X., Stein, Janet L., Stein, Gary S.
Interferon regulatory factors (IRFs) are transcriptional mediators of interferon-responsive signaling pathways that are involved in antiviral defense, immune response, and cell growth regulation. To...
Shopland, Lindsay S., Byron, Meg, Stein, Janet L., Lian, Jane B., Stein, Gary S., Lawrence, Jeanne B.
Interactions between Cajal bodies (CBs) and replication-dependent histone loci occur more frequently than for other mRNA-encoding genes, but such interactions are not seen with all alleles at a given...
Lian, Jane B., Stein, Janet L., Stein, Gary S., Montecino, Martin, Van Wijnen, Andre J., Javed, Amjad, ...
The vitamin D response element in the bone tissue-specific osteocalcin gene has served as a prototype for understanding molecular mechanisms regulating physiologic responsiveness of vitamin...
Marrow transplantation and targeted gene therapy to the skeleton (2000)
Lian, Jane B., Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J.
Treatment of genetic or degenerative diseases severely affecting the entire skeleton may necessitate gene therapy involving transplantation of multipotential marrow cells. The ability of in vitro...
Hoffmann, Heidi M., Green, Jack, Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.
Contributing to bone-specific expression of the osteocalcin gene is the promoter element OC Box I (-99 to -76), which binds both Hox proteins and another nonhomeodomain factor (designated OCBP for...
Staal, Ada, Enserink, Jorrit M., Stein, Janet L., Stein, Gary S., Van Wijnen, Andre J.
Transcriptional control at the G1/S-phase transition of the cell cycle requires functional interactions of multimeric promoter regulatory complexes that contain DNA binding proteins, transcriptional...
Transcriptional autoregulation of the bone related CBFA1/RUNX2 gene (2000)
Drissi, Hicham, Luc, Quyen, Shakoori, A. Rauf, Choi, Je-Yong, Terry, Anne, ...
The runt related transcription factor CBFA1 (AML3/PEBP2alphaA/RUNX2) regulates expression of several bone- and cartilage-related genes and is required for bone formation in vivo. The gene regulatory...
McNeil, Sandra Marie, Javed, Amjad, Harrington, Kimberly Stacy, Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., ...
Promyelocytic leukemia (PML) nuclear bodies are important components of nuclear architecture that are functionally linked to aberrant gene expression and disease. To understand the mechanisms that...
Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J.
BACKGROUND: The transplantation of multipotential bone marrow cells containing bone tissue specific promoter-controlled transgenes provides an efficacious approach to deliver therapeutic gene...
Transcriptional control of osteoblast differentiation (2000)
Lian, Jane B., Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J.
Intranuclear trafficking of transcription factors: implications for biological control (2000)
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Montecino, Martin A., Choi, Je-Yong, ...
The subnuclear organization of nucleic acids and cognate regulatory factors suggests that there are functional interrelationships between nuclear structure and gene expression. Nuclear proteins that...
Javed, Amjad, Guo, Bo, Hiebert, Scott W., Choi, Je-Yong, Green, Jack, Zhao, Shan-Chuan, ...
The Runt related transcription factors RUNX (AML/CBF(alpha)/PEBP2(alpha)) are key regulators of hematopoiesis and osteogenesis. Using co-transfection experiments with four natural promoters,...
Stein, Gary S., Montecino, Martin A., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B.
There is long-standing recognition that transformed and tumor cells exhibit striking alterations in nuclear morphology as well as in the representation and intranuclear distribution of nucleic acids...
Gordon, Jeffrey A., Pockwinse, Shirwin M., Stewart, F. Marc, Quesenberry, Peter J., Nakamura, Tatsuya, Croce, Carlo M., ...
Acute leukemias arise secondary to chromosomal aberrations that cause dysfunctions in gene regulation and regulatory factors. Significant differences in morphology between acute leukemic and...
Interrelationships of transcriptional machinery with nuclear architecture (2000)
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B.
There is increasing evidence for functional linkages between nuclear architecture and the regulation of gene expression. The nuclear matrix provides a paradigm for involvement of nuclear morphology...
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., McNeil, Sandra Marie, Pockwinse, Shirwin M.
Evidence is accruing that the architectural organization of nucleic acids and regulatory proteins within the cell nucleus support functional interrelationships between nuclear structure and gene...
McNeil, Sandra Marie, Zeng, Congmei, Harrington, Kimberly Stacy, Hiebert, Scott W., Lian, Jane B., Stein, Janet L., ...
Targeting of gene regulatory factors to specific intranuclear sites may be critical for the accurate control of gene expression. The acute myelogenous leukemia 8;21 (AML1/ETO) fusion protein is...
Van Gurp, Maria F., Pratap, Jitesh, Luong, Mai X., Javed, Amjad, Hoffmann, Heidi M., Giordano, Antonio, ...
Developmental control of bone tissue-specific genes requires positive and negative regulatory factors to accommodate physiological requirements for the expression or suppression of the encoded...
Insight into regulatory factor targeting to transcriptionally active subnuclear sites (1999)
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Javed, Amjad, McNeil, Sandra Marie, ...
Mechanisms that coordinate the spatial organization of genes and regulatory proteins within the three-dimensional context of nuclear architecture contribute to the sorting of regulatory information...
Tang, Lei, Guo, Bo, Javed, Amjad, Choi, Je-Yong, Hiebert, Scott W., Lian, Jane B., ...
Transcription factors of the acute myelogenous leukemia (AML)/polyoma enhancer-binding protein (PEBP2alpha)/core-binding factor alpha (CBFA) class are key transactivators of tissue-specific genes of...
Last, Thomas J., Van Wijnen, Andre J., De Ridder, Marleen C., Stein, Gary S., Stein, Janet L.
The homeodomain transcription factor CDP/cut contains four separate DNA binding domains and interacts with large segments of DNA. Thus, CDP/cut has the potential to function as an architectural...
Javed, Amjad, Gutierrez, Soraya E., Montecino, Martin, Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., ...
Three Cbfa motifs are strategically positioned in the bone-specific rat osteocalcin (rOC) promoter. Sites A and B flank the vitamin D response element in the distal promoter and sites B and C flank a...
Nuclear structure/gene expression interrelationships (1999)
Stein, Gary S., Van Wijnen, Andre J., Montecino, Martin A., Stein, Janet L., Lian, Jane B.
The cell cycle regulator p27kip1 contributes to growth and differentiation of osteoblasts (1999)
Drissi, Hicham, Hushka, Dennet, Aslam, Fauzia, Nguyen, Que, Buffone, Elizabeth, Koff, Andrew, ...
The cyclin-dependent kinase (cdk) inhibitors are key regulators of cell cycle progression. p27 and p21 are members of the Cip/Kip family of cdk inhibitors and regulate cell growth by inactivating...
Hou, Zhen, Nguyen, Que, Frenkel, Baruch, Nilsson, Susan K., Milne, Moira, Van Wijnen, Andre J., ...
Somatic gene therapies require targeted transfer of the therapeutic gene(s) into stem cells that proliferate and then differentiate and express the gene in a tissue-restricted manner. We have...
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Pockwinse, Shirwin M., McNeil, Sandra Marie
Components of nuclear architecture are functionally interrelated with control of gene expression. There is growing appreciation that multiple levels of nuclear organization integrate the regulatory...
Lee, Mi-Hye, Javed, Amjad, Kim, Hyun-Jung, Shin, Hong-In, Gutierrez, Soraya E., Choi, Je-Yong, ...
The bone morphogenetic protein (BMP)-2 is a potent osteoinductive signal, inducing bone formation in vivo and osteoblast differentiation from non-osseous cells in vitro. The runt domain-related...
Last, Thomas J., Van Wijnen, Andre J., Birnbaum, Mark J., Stein, Gary S., Stein, Janet L.
Multiple regulatory elements and intricate protein-DNA interactions mediate the transcription of the human histone H4 genes in a cell growth-dependent manner. Upon analysis of the regulatory elements...
Montecino, Martin A., Van Wijnen, Andre J., Lian, Jane B., Stein, Janet L., Stein, Gary S.
We have analyzed the linkage of protein phosphorylation to the remodeling of chromatin structure that accompanies transcriptional activity of the rat osteocalcin (OC) gene in bone-derived cells....
Regulated expression of the bone-specific osteocalcin gene by vitamins and hormones (1999)
Lian, Jane B., Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J.
Montecino, Martin A., Frenkel, Baruch, Van Wijnen, Andre J., Lian, Jane B., Stein, Gary S., Stein, Janet L.
Cells expressing the bone-specific osteocalcin (OC) gene exhibit two DNase I hypersensitive sites within the proximal (nt -170 to -70) and distal (nt -600 to -400) promoter. These sites overlap...
Lian, Jane B., Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J.
The bone tissue-specific osteocalcin gene remains one of a few genes that exhibits osteoblast-restricted expression. Over the last decade, characterization of the promoter regulatory elements and...
Linkages of nuclear architecture to biological and pathological control of gene expression (1999)
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Pockwinse, Shirwin M., McNeil, Sandra Marie
Functional interrelationships between components of nuclear architecture and control of gene expression are becoming increasingly evident. There is growing appreciation that multiple levels of...
Aslam, Fauzia, McCabe, Laura R., Frenkel, Baruch, Van Wijnen, Andre J., Stein, Gary S., Lian, Jane B., ...
Responsiveness of genes to steroid hormones is a complex process involving synergistic and/or antagonistic interactions between specific receptors and other nonreceptor transcription factors. Thus,...
Last, Thomas J., Birnbaum, Mark J., Van Wijnen, Andre J., Stein, Gary S., Stein, Janet L.
The coding region of the human histone H4 gene FO108 undergoes dynamic changes in chromatin structure that correlate with modifications in gene expression. Such structural alterations generally...
Aziz, Farah, Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
Cell cycle control of histone H4 gene transcription is mediated by the multipartite promoter domain H4-Site II, which supports transcriptional activation at the G1/S phase transition and modulates...
Tang, Lei, Guo, Bo, Van Wijnen, Andre J., Lian, Jane B., Stein, Janet L., Stein, Gary S., ...
A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-alpha2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the...
Vaughan, Patricia S., Staal, Ada, Albig, Werner, Doenecke, Detlef, Stein, Janet L., ...
Expression of many histone H4 genes is stringently controlled during the cell cycle to maintain a functional coupling of histone biosynthesis with DNA replication. The histone H4 multigene family...
Choi, Je-Yong, Van Wijnen, Andre J., Aslam, Fauzia, Leszyk, John D., Stein, Janet L., Stein, Gary S., ...
The protein composition of the nuclear matrix changes significantly as the osteoblast matures from a proliferating pre-osteoblast to an osteocyte embedded in a mineralized matrix. These matrix...
Nuclear structure--skeletal gene expression interrelationships (1998)
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B.
Using the osteocalcin gene as a paradigm for bone tissue-specific transcription, evidence is presented for functional linkage of nuclear architecture with developmental and steroid hormone-responsive...
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Pockwinse, Shirwin M., McNeil, Sandra Marie
Functional interrelationships between components of nuclear architecture and control of gene expression are becoming increasingly evident. In this article we focus on the concept that association of...
TGF-beta1 modifications in nuclear matrix proteins of osteoblasts during differentiation (1998)
Lindenmuth, Danielle M., Van Wijnen, Andre J., Penman, Sheldon, Stein, Janet L., Stein, Gary S., Lian, Jane B.
Nuclear matrix protein (NMP) composition of osteoblasts shows distinct two-dimensional gel electrophoretic profiles of labeled proteins as a function of stages of cellular differentiation. Because...
Shalhoub, Victoria, Aslam, Fauzia, Breen, Ellen C., Van Wijnen, Andre J., Bortell, Rita, Stein, Gary S., ...
We have examined the contribution of transcriptional mechanisms to the pleiotropic effects of glucocorticoids on basal and vitamin D stimulated expression of the developmentally regulated...
Aziz, Farah, Van Wijnen, Andre J., Vaughan, Patricia S., Wu, Shujian, Shakoori, A. Rauf, Lian, Jane B., ...
Maximal transcription of a prototypical cell cycle controlled histone H4 gene requires a proliferation-specific in vivo genomic protein/DNA interaction element, Site II. Three sequence-specific...
Nuclear matrix associated DNA-binding proteins of ocular lens epithelial cells (1998)
Bagchi, Mihir, Ansari, Shamim A., Lindenmuth, Danielle M., Van Wijnen, Andre J., Lian, Jane B., Stein, Janet L., ...
Association of transcription factors with the nuclear matrix represents a mechanism by which nuclear architecture may influence transcriptional control of gene expression. This investigation examines...
Zeng, Congmei, McNeil, Sandra Marie, Pockwinse, Shirwin M., Nickerson, Jeffrey A., Shopland, Lindsay S., Lawrence, Jeanne B., ...
The AML/CBFalpha runt transcription factors are key regulators of hematopoietic and bone tissue-specific gene expression. These factors contain a 31-amino acid nuclear matrix targeting signal that...
McNeil, Sandra Marie, Guo, Bo, Stein, Janet L., Lian, Jane B., Bushmeyer, Sarah M., Seto, Edward, ...
The multifunctional transcription factor YY1 is associated with the nuclear matrix. In osteoblasts, the interaction of several nuclear matrix-associated transcription factors with the bone specific...
Cell cycle regulation of histone H4 gene transcription requires the oncogenic factor IRF-2 (1998)
Vaughan, Patricia S., Aziz, Farah, Harada, Hisashi, Taniguchi, Tadatsugu, Van Wijnen, Andre J., ...
Histone genes display a peak in transcription in early S phase and are ideal models for cell cycle-regulated gene expression. We have previously shown that the transcription factor interferon...
Guo, Bo, Stein, Janet L., Van Wijnen, Andre J., Stein, Gary S.
Proteins of the ATF/CREB class of transcription factors stimulate gene expression of several cell growth-related genes through protein kinase A-related cAMP response elements. The promoter activity...
Ryoo, Hyun-Mo, Hoffmann, Heidi M., Beumer, T. L., Frenkel, Baruch, Towler, Dwight A., Stein, Gary S., ...
Two homeotic genes, Dlx and Msx, appear to regulate development of mineralized tissues, including bone, cartilage, and tooth. Expression of Msx-1 and Msx-2 has been studied during development of the...
Van Wijnen, Andre J., Cooper, Cathleen L., Odgren, Paul R., Aziz, Farah, De Luca, Antonio, Shakoori, A. Rauf, ...
The histone H4 gene promoter provides a paradigm for defining transcriptional control operative at the G1/S phase transition point in the cell cycle. Transcription of the cell cycle-dependent histone...
Birnbaum, Mark J., Van Zundert, Brigitte, Vaughan, Patricia S., Whitmarsh, Alan J., Van Wijnen, Andre J., Davis, Roger J., ...
IRF2 is a transcription factor, possessing oncogenic potential, responsible for both the repression of growth-inhibiting genes (interferon) and the activation of cell cycle-regulated genes (histone...
Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L.
Influences of promoter regulatory elements that are responsive to basal and tissue-restricted transactivation factors, steroid hormones, growth factors and other physiologic mediators has provided...
Banerjee, Chaitali, McCabe, Laura R., Choi, Je-Yong, Hiebert, Scott W., Stein, Janet L., Stein, Gary S., ...
The AML/CBFA family of runt homology domain (rhd) transcription factors regulates expression of mammalian genes of the hematopoietic lineage. AML1, AML2 and AML3 are the three AML genes identified to...
Lindenmuth, Danielle M., Van Wijnen, Andre J., Hiebert, Scott W., Stein, Janet L., Lian, Jane B., Stein, Gary S.
The subnuclear location of transcription factors may functionally contribute to the regulation of gene expression. Several classes of gene regulators associate with the nuclear matrix in a cell type,...
Zeng, Congmei, Van Wijnen, Andre J., Stein, Janet L., Meyers, Shari, Sun, Wuhua, Shopland, Lindsay S., ...
Transcription factors of the AML (core binding factor-alpha/polyoma enhancer binding protein 2) class are key transactivators of tissue-specific genes of the hematopoietic and bone lineages....
Lian, Jane B., Shalhoub, Victoria, Aslam, Fauzia, Frenkel, Baruch, Green, Jack, Hamrah, Michael, ...
The mouse MC3T3-E1 cell line is nontumorigenic and undergoes a typical program of osteoblast differentiation in vitro, producing a bone-like mineralized extracellular matrix. We report responses of...
Frenkel, Baruch, Capparelli, Casey, Van Auken, Moira, Baran, Daniel T., Bryan, J., Stein, Janet L., ...
The bone-specific osteocalcin gene is a well established marker of osteoblast activity. We have studied osteocalcin transcription in transgenic mice carrying rat osteocalcin promoter-chloramphenicol...
Vaughan, Patricia S., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
Interferon-regulatory factors (IRFs) are a related family of proteins originally identified by their ability to bind a DNA sequence found in the beta-interferon gene and many interferon-stimulated...
Pockwinse, Shirwin M., Krockmalnic, Gabriela, Doxsey, Stephen J., Nickerson, Jeffrey A., Lian, Jane B., Van Wijnen, Andre J., ...
The cell cycle regulating Cdc2 protein kinase helps orchestrate cell cycle dependent changes in cell structure and function. This report shows that Cdc2 is localized to the centrosome region and is...
Guo, Bo, Aslam, Fauzia, Van Wijnen, Andre J., Roberts, Stefan G. E., Frenkel, Baruch, Green, Michael R., ...
The responsiveness of genes to steroid hormones is principally mediated by functional interactions between DNA-bound hormone receptors and components of the transcriptional initiation machinery,...
Ryoo, Hyun-Mo, Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Stein, Gary S.
Fetal rat calvarial-derived osteoblasts in vitro (ROB) reinitiate a developmental program from growth to differentiation concomitant with production of a bone tissue-like organized extracellular...
Staal, Ada, Van Wijnen, Andre J., Birkenhager, Jan C., Pols, Huibert A. P., Prahl, Jean, DeLuca, Hector F., ...
The 1 alpha,25-dihydroxyvitamin D3 (VD3)-dependent stimulation of osteocalcin (OC) and osteopontin (OP) gene transcription in bone tissue is mediated by interactions of trans-activating factors with...
Van Wijnen, Andre J., Van Gurp, Maria F., De Ridder, Marleen C., Tufarelli, Cristina, Last, Thomas J., Birnbaum, Mark J., ...
Transcription of the genes for the human histone proteins H4, H3, H2A, H2B, and H1 is activated at the G1/S phase transition of the cell cycle. We have previously shown that the promoter complex...
McCabe, Laura R., Banerjee, Chaitali, Kundu, R., Harrison, Robert J., Dobner, Paul R., Stein, Janet L., ...
Developmental studies of oncogene expression implicate the Fos and Jun family of transcription factors in the regulation of bone growth and differentiation. Promoters of many developmentally...
Stein, Gary S., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A.
Multiple levels of nuclear structure contribute to functional interrelationships with transcriptional control in vivo. The linear organization of gene regulatory sequences is necessary but...
Banerjee, Chaitali, Hiebert, Scott W., Stein, Janet L., Lian, Jane B., Stein, Gary S.
Tissue and cell-type specific expression of the rat osteocalcin (rOC) gene involves the interplay of multiple transcriptional regulatory factors. In this report we demonstrate that AML-1 (acute...
Banerjee, Chaitali, Stein, Janet L., Van Wijnen, Andre J., Frenkel, Baruch, Lian, Jane B., Stein, Gary S.
Osteocalcin (OC), a bone specific protein expressed during differentiation and mineralization of the bone extracellular matrix, is down-regulated upon treatment with transforming growth factor...
Hoffmann, Heidi M., Beumer, T. L., Rahman, Shamim, McCabe, Laura R., Banerjee, Chaitali, Aslam, Fauzia, ...
Bone-specific expression of the osteocalcin gene is transcriptionally controlled. Deletion analysis of osteocalcin promoter sequences by transient transfection of osseous (ROS 17/2.8) and nonosseous...
Staal, Ada, Van Wijnen, Andre J., Desai, Rajesh K., Pols, Huibert A. P., Birkenhager, Jan C., DeLuca, Hector F., ...
Osteocalcin and osteopontin are noncollagenous proteins secreted by osteoblasts and regulated by a complex interplay of systemic and locally produced factors, including growth factors and steroid...
Transcriptional control of osteoblast growth and differentiation (1996)
Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Montecino, Martin A.
Osteoblast differentiation is a multistep series of events modulated by an integrated cascade of gene expression that initially supports proliferation and the sequential expression of genes...
Frenkel, Baruch, Montecino, Martin A., Green, Jack, Aslam, Fauzia, Desai, R., Banerjee, Chaitali, ...
Osteocalcin (OC) is a bone-specific vitamin D- responsive protein that is developmentally expressed during osteoblast differentiation. In transient transfection assays, as little as approximately 0.1...
Control of cell cycle regulated histone genes during proliferation and differentiation (1996)
Stein, Janet L., Van Wijnen, Andre J., Lian, Jane B., Stein, Gary S.
Proliferation is a requirement during the initial stages of cell and tissue specialization. In most biological systems the down-regulation of proliferation is necessary for initiation of key steps in...
Lian, Jane B., Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J., McCabe, Laura R., Banerjee, Chaitali, ...
Characterization of regulatory sequences and their cognate binding factors in the bone-specific osteocalcin (OC) gene promoter has provided insight into mechanisms that control expression of the gene...
Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J., Lian, Jane B.
The histone gene is a paradigm for transcriptional control at the G1/S phase transition point in the cell cycle. The histone gene promoter provides a blueprint for integration of regulatory signals...
Sp1 trans-activation of cell cycle regulated promoters is selectively repressed by Sp3 (1995)
Birnbaum, Mark J., Van Wijnen, Andre J., Odgren, Paul R., Last, Thomas J., Suske, Guntram, Stein, Gary S., ...
The transcription factor Sp1 plays a key role in the activation of many cellular and viral gene promoters, including those that are regulated during the cell cycle. However, recent evidence indicates...
Desai, Rajesh K., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.
The 1,25-dihydroxyvitamin D3 (vitamin D) receptor (VDR) is a key trans-activating protein that mediates calcium regulation as well as cellular proliferation and differentiation. Phosphorylation of...
The nuclear matrix protein NMP-1 is the transcription factor YY1 (1995)
Guo, Bo, Odgren, Paul R., Van Wijnen, Andre J., Last, Thomas J., Nickerson, Jeffrey A., Penman, Sheldon, ...
NMP-1 was initially identified as a nuclear matrix-associated DNA-binding factor that exhibits sequence-specific recognition for the site IV regulatory element of a histone H4 gene. This distal...
Smith, Elisheva, Frenkel, Baruch, Schlegel, Robert, Giordano, Antonio, Lian, Jane B., Stein, Janet L., ...
The representation of cyclins and cyclin-dependent kinases (cdks) was analyzed during progressive development of the bone cell phenotype in cultures of normal diploid rat calvarial osteoblasts. Three...
Merriman, Harold L., Van Wijnen, Andre J., Hiebert, Scott W., Bidwell, Joseph P., Fey, Edward G., Lian, Jane B., ...
The nuclear matrix protein, NMP-2, was originally identified as an osteoblast-specific DNA-binding complex localized exclusively to the nuclear matrix. NMP-2 was shown to recognize two binding sites,...
Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2 (1995)
Vaughan, Patricia S., Aziz, Farah, Van Wijnen, Andre J., Wu, Shujian, Harada, Hisashi, Taniguchi, Tadatsugu, ...
The human histone H4 gene FO108 is regulated during the cell cycle with a peak in transcription during early S phase. The cell-cycle element (CCE) required for H4 histone activation is a sequence of...
Molecular mechanisms mediating control of cell cycle and cell growth (1995)
Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J., Lian, Jane B., Quesenberry, Peter J.
Bipartite structure of the proximal promoter of a human H4 histone gene (1995)
Wright, Kenneth Lynn, Birnbaum, Mark J., Van Wijnen, Andre J., Stein, Gary S., Stein, Janet L.
The proximal promoter of the human H4 histone gene FO108 contains two regions of in vivo protein-DNA interaction, Sites I and II. Electrophoretic mobility shift assays using a radiolabeled DNA probe...
Aslam, Fauzia, Shalhoub, Victoria, Van Wijnen, Andre J., Banerjee, Chaitali, Bortell, Rita, Shakoori, A. Rauf, ...
Previous studies identified several glucocorticoid response elements (GREs) in the 5'-promoter region of the rat osteocalcin (OC) gene by purified receptor binding. The present study addresses...
Kroeger, Paul E., Van Wijnen, Andre J., Pauli, Urs, Wright, Kenneth Lynn, Stein, Gary S., Stein, Janet L.
To assess systematically the structural and functional aspects of histone gene transcription within a chromosomal context, we stably integrated an extensive set of human histone H4 gene constructs...
Contributions of nuclear architecture to transcriptional control (1995)
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Montecino, Martin A.
Three parameters of nuclear structure contribute to transcriptional control. The linear representation of promoter elements provides competency for physiological responsiveness within the contexts of...
Breen, Ellen C., Van Wijnen, Andre J., Lian, Jane B., Stein, Gary S., Stein, Janet L.
The steroid hormone vitamin D is a principal mediator of skeletal homeostasis. 1,25-Dihydroxyvitamin D3 treatment of ROS 17/2.8 osteoblast-like cells results in a ligand-dependent increase in...
Hoffmann, Heidi M., Catron, Katrina M., Van Wijnen, Andre J., McCabe, Laura R., Lian, Jane B., Stein, Gary S., ...
The OC box of the rat osteocalcin promoter (nt -99 to -76) is the principal proximal regulatory element contributing to both tissue-specific and developmental control of osteocalcin gene expression....
Frenkel, Baruch, Montecino, Martin A., Stein, Janet L., Lian, Jane B., Stein, Gary S.
The osteocalcin (OC) silencer is a unique example of exonic sequences contributing to negative transcriptional control of mammalian gene expression. In this paper we demonstrate, using a reporter...
McCabe, Laura R., Last, Thomas J., Lynch, Maureen P., Lian, Jane B., Stein, Janet L., Stein, Gary S.
Establishing regulatory mechanisms that mediate proliferation of osteoblasts while restricting expression of genes associated with mature bone cell phenotypic properties to post-proliferative cells...
Shakoori, A. Rauf, Van Wijnen, Andre J., Bortell, Rita, Owen, T. A., Stein, Janet L., Lian, Jane B., ...
Vitamin D responsive transcription of the bone-specific osteocalcin gene differs markedly in osteosarcoma cells and normal diploid osteoblasts. In osteoblasts the osteocalcin gene is transcribed, and...
Van Wijnen, Andre J., Lian, Jane B., Stein, Janet L., Stein, Gary S.
Cell cycle and growth control of the DNA binding and transactivation functions of regulatory factors provides a direct mechanism by which cells may coordinate transcription of a multitude of genes in...
Ramsey-Ewing, Anna L., Van Wijnen, Andre J., Stein, Gary S., Stein, Janet L.
Histone gene expression is cell cycle regulated at the transcriptional and the post-transcriptional levels. Upon entry into S phase, histone gene transcription is stimulated 2- to 5-fold and peaks...
Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Bidwell, Joseph P., Montecino, Martin A.
During the past several years it has become increasingly evident that the three-dimensional organization of the nucleus plays a critical role in transcriptional control. The principal theme of this...
Bidwell, Joseph P., Van Wijnen, Andre J., Banerjee, Chaitali, Fey, Edward G., Merriman, Harold L., Penman, Sheldon, ...
PTH is a mediator of skeletal development and remodeling that influences gene expression in osteoblastic cells. It is well established that PTH modulates the activity of membrane-associated second...
Subnuclear distribution of the vitamin D receptor (1994)
Bidwell, Joseph P., Van Wijnen, Andre J., Fey, Edward G., Merriman, Harold L., Penman, Sheldon, Stein, Janet L., ...
The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D-responsive control of gene expression in ROS 17/2.8...
Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J., Lian, Jane B.
Histone gene expression is restricted to the S-phase of the cell cycle. Control is at multiple levels and is mediated by the integration of regulatory signals in response to cell cycle progression...
Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells (1994)
Bidwell, Joseph P., Fey, Edward G., Van Wijnen, Andre J., Penman, Sheldon, Stein, Janet L., Lian, Jane B., ...
Interrelationships between nuclear architecture and gene expression were examined by comparing the representation of nuclear matrix proteins in ROS 17/2.8 rat and MG-63 human osteosarcoma cells with...
Frenkel, Baruch, Mijnes, Jolanda, Aronow, Michael A., Zambetti, Gerard, Banerjee, Chaitali, Stein, Janet L., ...
Osteocalcin (OC) is a bone-specific protein which is expressed postproliferatively by osteoblasts during late stages of differentiation. We have found that a silencer element is present within the...
Multiple copies of the bone-specific osteocalcin gene in mouse and rat (1993)
Rahman, Shamim, Oberdorf, Annette M., Montecino, Martin A., Tanhauser, Susan M., Lian, Jane B., Stein, Gary S., ...
The osteocalcin (OC) gene was initially described as a single copy gene encoding the bone specific vitamin K dependent and vitamin D regulated protein. We report here the presence of multiple copies...
Van Wijnen, Andre J., Bidwell, Joseph P., Fey, Edward G., Penman, Sheldon, Lian, Jane B., Stein, Janet L., ...
The association of DNA binding proteins with the nuclear matrix may be related to a functional role of this subcellular structure in chromatin organization and gene regulation. In this study, nuclear...
Van Wijnen, Andre J., Last, Thomas J., Bortell, Rita, Stein, Janet L., Lian, Jane B., ...
Cell density-induced growth inhibition of osteosarcoma cells (ROS 17/2.8) results in the shutdown of proliferation-specific histone H4 and H2B genes and the concomitant up-regulation of several...
Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components (1993)
Bidwell, Joseph P., Van Wijnen, Andre J., Fey, Edward G., Dworetzky, Steven I., Penman, Sheldon, Stein, Janet L., ...
The nuclear matrix appears to play an important role in developmental gene expression during osteoblast differentiation. To better understand this role, we examined nuclear matrix DNA-binding...
Bortell, Rita, Owen, T. A., Shalhoub, Victoria, Heinrichs, A, Aronow, Michael A., Rochette-Egly, C, ...
The bone-specific osteocalcin (OC) gene is transcribed only after completion of proliferation in normal diploid calvarial-derived osteoblasts during extracellular matrix mineralization. In contrast,...
Owen, T. A., Bortell, Rita, Shalhoub, Victoria, Heinrichs, A, Stein, Janet L., Stein, Gary S., ...
In the osteocalcin (OC) gene promoter, both independent positive and negative regulatory elements, as well as others with contiguous [TATA/glucocorticoid-responsive elements (GRE)] or overlapping...
Bortell, Rita, Van Wijnen, Andre J., Ramsey-Ewing, Anna L., Stein, Gary S., Stein, Janet L.
The aim of this study was to address whether there is a fundamental difference in regulation of histone gene expression in cells that have become quiescent but retain the ability to proliferate,...
Bortell, Rita, Owen, T. A., Bidwell, Joseph P., Gavazzo, P., Breen, Ellen C., Van Wijnen, Andre J., ...
The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of...
Van Wijnen, Andre J., Lian, Jane B., Stein, Janet L., Stein, Gary S.
Transcriptional regulation of vertebrate histone genes during the cell cycle is mediated by several factors interacting with a series of cis-acting elements located in the 5' regions of these genes....
Gerbaulet, Stephan P., Van Wijnen, Andre J., Aronin, Neil, Tassinari, Melissa S., Lian, Jane B., Stein, Janet L., ...
In vivo regulation of cell cycle dependent human histone gene expression was examined in transgenic mice using a fusion construct containing 6.5 kB of a human H4 promoter linked to the...
Sequence-specific DNA-binding proteins are components of a nuclear matrix-attachment site (1992)
Dworetzky, Steven I., Wright, Kenneth Lynn, Fey, Edward G., Penman, Sheldon, Lian, Jane B., Stein, Janet L., ...
We have identified a nuclear matrix-attachment region within an upstream element of a human H4 histone gene promoter. Nuclear matrix proteins, isolated and solubilized from HeLa S3 cells, were found...
Van Wijnen, Andre J., Carter, Ruth, Soprano, Kenneth J., Lian, Jane B., Stein, Gary S., ...
Upstream sequences of the H4 histone gene FO108 located between nt -418 to -213 are stimulatory for in vivo transcription. This domain contains one protein/DNA interaction site (H4-Site III) that...
Regulation of histone gene expression (1992)
Stein, Gary S., Stein, Janet L., Van Wijnen, Andre J., Lian, Jane B.
Histone genes are expressed during the S phase of the cell cycle. Control is at multiple levels and is mediated by the integration of regulatory signals in response to cell-cycle progression and the...
Wright, Kenneth Lynn, Dell'Orco, Robert T., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
The proliferation-specific transcription factor complex HiNF-D interacts with sequence specificity in a proximal promoter element of the human H4 histone gene FO108, designated Site II. The occupancy...
Van Wijnen, Andre J., Bidwell, Joseph P., Lian, Jane B., Stein, Janet L., Stein, Gary S.
We describe a rapid method for initial localization of protein/DNA-binding sites in large DNA segments, even when biological information is limited. The procedure combines the gel retardation assay...
Van Wijnen, Andre J., Lian, Jane B., Stein, Janet L., Stein, Gary S.
Protein/DNA interactions of the H3-ST519 histone gene promoter were analyzed in vitro. Using several assays for sequence specificity, we established binding sites for ATF/AP1-, CCAAT-, and HiNF-D...
Van Wijnen, Andre J., Owen, T. A., Holthuis, Joost, Lian, Jane B., Stein, Janet L., Stein, Gary S.
Coordinate transcriptional control of replication-dependent human H4, H3, and H1 histone genes was studied by comparing levels of H3 and H1 histone promoter binding activities with those of H4...
Van Wijnen, Andre J., Ramsey-Ewing, Anna L., Bortell, Rita, Owen, T. A., Lian, Jane B., Stein, Janet L., ...
Cell cycle regulated gene expression was studied by analyzing protein/DNA interactions occurring at the H4-Site II transcriptional element of H4 histone genes using several approaches. We show that...
Van Wijnen, Andre J., Choi, Theodore K., Owen, T. A., Wright, Kenneth Lynn, Lian, Jane B., Jaenisch, Rudolf, ...
Regulation of the cell cycle-controlled histone gene promoter factor HiNF-D was examined in vivo. Proliferative activity was measured by DNA replication-dependent histone mRNA levels, and HiNF-D...
Dworetzky, Steven I., Fey, Edward G., Penman, Sheldon, Lian, Jane B., Stein, Janet L., Stein, Gary S.
Primary cultures of fetal rat calvarial osteoblasts undergo a developmental sequence with respect to the temporal expression of genes encoding osteoblast phenotypic markers. Based on previous...
Markose, Elizabeth R., Stein, Janet L., Stein, Gary S., Lian, Jane B.
By the combined use of DNase I footprinting, electrophoretic mobility-shift assay, and methylation interference analysis, we have identified a series of sequence-specific protein-DNA interactions in...
Stein, Gary S., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Wright, Kenneth Lynn, Pauli, Urs
Histone proteins are preferentially synthesized during the S-phase of the cell cycle, and the temporal and functional coupling of histone gene expression with DNA replication is mediated at both the...
Van Wijnen, Andre J., Wright, Kenneth Lynn, Lian, Jane B., Stein, Janet L., Stein, Gary S.
The proximal promoter of the human H4 histone gene F0108 contains two in vivo protein binding domains, sites I and II. In this report we show that these sequences interact with three nuclear factors:...
Stein, Gary S., Lian, Jane B., Stein, Janet L., Briggs, Robert C., Shalhoub, Victoria, Wright, Kenneth Lynn, ...
Two sites of protein-DNA interaction have been identified in vivo and in vitro in the proximal promoter regions of an H4 and an H3 human histone gene. In proliferating cells, these genes are...
Typescript.
Collart, David G., Wright, Kenneth Lynn, Van Wijnen, Andre J., Ramsey-Ewing, Anna L., Lian, Jane B., Stein, Janet L., ...
The human H1 histone gene FNC16 resides in a 2.7-kb EcoRI fragment present in a histone gene cluster that also contains one copy of each of the core (H2A, H2B, H3, and H4) histone genes. The cap site...
Human H1 histone gene promoter CCAAT box binding protein HiNF-B is a mosaic factor (1988)
Van Wijnen, Andre J., Massung, Robert F., Stein, Janet L., Stein, Gary S.
Vertebrate histone gene promoters in many cases contain an upstream element, 5'dCCAAT, that has been implicated in modulating the efficiency of transcription of a broad spectrum of genes. We have...
Van Wijnen, Andre J., Wright, Kenneth Lynn, Massung, Robert F., Gerretsen, Martijn, Stein, Janet L., Stein, Gary S.
The 5' region of a cell cycle regulated human H1 histone gene appears to contain at least six promoter DNA elements that are shared with some, but not all human core (H2A, H2B, H3 and H4) histone...
Lawrence, Jeanne B., Singer, Robert H., Villnave, Carol A., Stein, Janet L., Stein, Gary S.
We have used in situ hybridization to study the intracellular distribution of mRNAs for cell cycle-dependent core and H1 histone proteins in human WI-38 fibroblasts. Because histones are abundant...
Van Wijnen, Andre J., Wright, Kenneth L., Massung, Robert F., Gerretsen, Martijn, Stein, Janet L., Stein, Gary S.
The 5' region of a cell cycle regulated human H1 histone gene appears to contain at least six promoter DNA elements that are shared with some, but not all human core (H2A, H2B, H3 and H4) histone...
A rat histone H4 gene closely associated with the testis-specific H1t gene (1987)
Grimes, Sidney R., Weisz-Carrington, Paul, Daum, Henry A., Smith, John M., Green, Linda G., Wright, Kenneth Lynn, ...
A rat histone H4 gene closely associated with the testis-specific H1t gene was isolated by screening the Sargent-Bonner rat genomic library using cloned human histone genes as probes. Both the H4...
Helms, Sherron R., Van Wijnen, Andre J., Kroeger, Paul E., Shiels, A., Stewart, C., Hirshman, J., ...
We have identified a segment of DNA in the region 6,500 nucleotides upstream from a cell-cycle-dependent human H4 histone gene (pF0108A) which exhibits properties of an enhancer element. This distal...
Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
A nuclear protein with affinity for the 5' flanking region of a cell cycle dependent human H4 histone gene has been partially purified from nuclear extracts of human HeLa S3 cells. The region...
Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
A nuclear protein with affinity for the 5′ flanking region of a cell cycle dependent human H4 histone gene has been partially purified from nuclear extracts of human HeLa S3 cells. The region...
Cell cycle-dependent expression of a stable episomal human histone gene in a mouse cell (1986)
Green, Linda G., Schlaffer, I., Wright, Kenneth Lynn, Moreno, M. L., Berand, D., Hager, Gordon L., ...
We have constructed a recombinant plasmid that includes a cell cycle-dependent human H4 histone gene with 650 base pairs of 5' and 900 base pairs of 3' flanking sequences and the 69% transforming...
Nuclear protein kinase activities during the cell cycle of HeLa S3 cells (1979)
Phillips, Ian R., Shephard, Elizabeth A., Stein, Janet L., Kleinsmith, Lewis J., Stein, Gary S.
To ascertain the activity and substrate specificity of nuclear protein kinases during various stages of the cell cycle of HeLa S3 cells, a nuclear phosphoprotein-enriched sample was extracted from...
Partial fractionation and characterization of nuclear protein kinases in HeLa S3 cells (1979)
Thomson, Judith A., Mon, Manuel J., Stein, Janet L., Duval, Kathryn A., Kleinsmith, Lewis J., Stein, Gary S.
Five fractions of phosphoprotein kinase activity were separated by phosphocellulose chromatography of nuclear non-histone phosphoproteins isolated from exponentially-growing HeLa S3 cells. Each...
Zeng, Congmei, McNeil, Sandra, Pockwinse, Shirwin, Nickerson, Jeffrey, Shopland, Lindsay, Lawrence, Jeanne B., ...
The AML/CBFα runt transcription factors are key regulators of hematopoietic and bone tissue-specific gene expression. These factors contain a 31-amino acid nuclear matrix targeting signal that...
Pockwinse, Shirwin M., Krockmalnic, Gabriela, Doxsey, Stephen J., Nickerson, Jeffrey, Lian, Jane B., Van Wijnen, André J., ...
The cell cycle regulating Cdc2 protein kinase helps orchestrate cell cycle dependent changes in cell structure and function. This report shows that Cdc2 is localized to the centrosome region and is...
Zeng, Congmei, Van Wijnen, André J., Stein, Janet L., Meyers, Shari, Sun, Wuhua, Shopland, Lindsay, ...
Transcription factors of the AML (core binding factor-α/polyoma enhancer binding protein 2) class are key transactivators of tissue-specific genes of the hematopoietic and bone lineages. Alternative...
Hou, Zhen, Nguyen, Que, Frenkel, Baruch, Nilsson, Susan K., Milne, Moira, Van Wijnen, André J., ...
Somatic gene therapies require targeted transfer of the therapeutic gene(s) into stem cells that proliferate and then differentiate and express the gene in a tissue-restricted manner. We have...
McNeil, Sandra, Zeng, Congmei, Harrington, Kimberly S., Hiebert, Scott, Lian, Jane B., Stein, Janet L., ...
Targeting of gene regulatory factors to specific intranuclear sites may be critical for the accurate control of gene expression. The acute myelogenous leukemia 8;21 (AML1/ETO) fusion protein is...
Shopland, Lindsay S., Byron, Meg, Stein, Janet L., Lian, Jane B., Stein, Gary S., Lawrence, Jeanne B.
Interactions between Cajal bodies (CBs) and replication-dependent histone loci occur more frequently than for other mRNA-encoding genes, but such interactions are not seen with all alleles at a given...
Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Zaidi, S. Kaleem, Xing, Lianping, Balint, Eva, ...
Runx (Cbfa/AML) transcription factors are critical for tissue-specific gene expression. A unique targeting signal in the C terminus directs Runx factors to discrete foci within the nucleus. Using...
Javed, Amjad, Gutierrez, Soraya, Montecino, Martin, Van Wijnen, André J., Stein, Janet L., Stein, Gary S., ...
Three Cbfa motifs are strategically positioned in the bone-specific rat osteocalcin (rOC) promoter. Sites A and B flank the vitamin D response element in the distal promoter and sites B and C flank a...
Javed, Amjad, Barnes, George L., Jasanya, B. O., Stein, Janet L., Gerstenfeld, Louis, Lian, Jane B., ...
Expression of the bone sialoprotein (BSP) gene, a marker of bone formation, is largely restricted to cells in mineralized tissues. Recent studies have shown that the Cbfa1 (also known as Runx2,...
Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites
Zaidi, Sayyed K., Sullivan, Andrew J., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.
Runx factors control lineage commitment and are transcriptional effectors of Smad signaling. Genetic defects in these pathways interfere with normal development. The in situ localization of Runx and...
Luong, Mai X., Van Der Meijden, Caroline M., Xing, DongXia, Hesselton, Ruth, Monuki, Edwin S., Jones, Stephen N., ...
Murine CDP/Cux, a homologue of the Drosophila Cut homeoprotein, modulates the promoter activity of cell cycle-related and cell-type-specific genes. CDP/Cux interacts with histone gene promoters as...
Multiple subnuclear targeting signals of the leukemia-related AML1/ETO and ETO repressor proteins
Barseguian, Karina, Lutterbach, Bart, Hiebert, Scott W., Nickerson, Jeffrey, Lian, Jane B., Stein, Janet L., ...
Leukemic disease can be linked to aberrant gene expression. This often is the result of molecular alterations in transcription factors that lead to their misrouting within the nucleus. The acute...
Hovhannisyan, Hayk, Cho, Brian, Mitra, Partha, Montecino, Martin, Stein, Gary S., Van Wijnen, Andre J., ...
During the shutdown of proliferation and onset of differentiation of HL-60 promyelocytic leukemia cells, expression of the cell cycle-dependent histone genes is downregulated at the level of...
Sierra, Jose, Villagra, Alejandro, Paredes, Roberto, Cruzat, Fernando, Gutierrez, Soraya, Javed, Amjad, ...
p300 is a multifunctional transcriptional coactivator that serves as an adapter for several transcription factors including nuclear steroid hormone receptors. p300 possesses an intrinsic histone...
Mitra, Partha, Xie, Rong-Lin, Medina, Ricardo, Hovhannisyan, Hayk, Zaidi, S. Kaleem, Wei, Yue, ...
At the G1/S phase cell cycle transition, multiple histone genes are expressed to ensure that newly synthesized DNA is immediately packaged as chromatin. Here we have purified and functionally...
Zaidi, Sayyed K., Young, Daniel W., Pockwinse, Shirwin M., Javed, Amjad, Lian, Jane B., Stein, Janet L., ...
Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately...
Zaidi, Sayyed K, Sullivan, Andrew J, Medina, Ricardo, Ito, Yoshiaki, Van Wijnen, Andre J, Stein, Janet L, ...
Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of...
Hassan, Mohammad Q., Javed, Amjad, Morasso, Maria I., Karlin, Jeremy, Montecino, Martin, Van Wijnen, Andre J., ...
Genetic studies show that Msx2 and Dlx5 homeodomain (HD) proteins support skeletal development, but null mutation of the closely related Dlx3 gene results in early embryonic lethality. Here we find...
Paredes, Roberto, Arriagada, Gloria, Cruzat, Fernando, Villagra, Alejandro, Olate, Juan, Zaidi, Kaleem, ...
Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1α,25-dihydroxyvitamin D3 via its...
Javed, Amjad, Barnes, George L., Pratap, Jitesh, Antkowiak, Tomasz, Gerstenfeld, Louis C., Van Wijnen, Andre J., ...
Runx transcription factors comprise a family of proteins that are essential for organogenesis. A unique nuclear matrix-targeting signal in the C terminus directs these factors to their appropriate...
Vradii, Diana, Zaidi, Sayyed K., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
The multifunctional C terminus of the hematopoietic AML1 transcription factor interacts with coregulatory proteins, supports the convergence and integration of physiological signals, and contains the...
Lederman, Sally Ann, Rauh, Virginia, Weiss, Lisa, Stein, Janet L., Hoepner, Lori A., Becker, Mark, ...
The effects of prenatal exposure to pollutants from the World Trade Center (WTC) disaster on fetal growth and subsequent health and development of exposed children remain a source of concern. We...
Pratap, Jitesh, Javed, Amjad, Languino, Lucia R., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., ...
The Runx2 (Cbfa1/AML3) transcription factor and matrix metalloproteinase 9 (MMP9) are key regulators of growth plate maturation and bone formation. The genes for both proteins are characteristic...
The dynamic organization of gene-regulatory machinery in nuclear microenvironments
Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin, ...
Nuclear components are functionally linked with the dynamic temporal and spatial compartmentalization, sorting and integration of regulatory information to facilitate its selective use. For example,...
Miele, Angela, Braastad, Corey D., Holmes, William F., Mitra, Partha, Medina, Ricardo, Xie, Ronglin, ...
Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the...
Zeng, Congmei, McNeil, Sandra, Pockwinse, Shirwin, Nickerson, Jeffrey, Shopland, Lindsay, Lawrence, Jeanne B., ...
The AML/CBFα runt transcription factors are key regulators of hematopoietic and bone tissue-specific gene expression. These factors contain a 31-amino acid nuclear matrix targeting signal that...
Pockwinse, Shirwin M., Krockmalnic, Gabriela, Doxsey, Stephen J., Nickerson, Jeffrey, Lian, Jane B., Van Wijnen, André J., ...
The cell cycle regulating Cdc2 protein kinase helps orchestrate cell cycle dependent changes in cell structure and function. This report shows that Cdc2 is localized to the centrosome region and is...
Zeng, Congmei, Van Wijnen, André J., Stein, Janet L., Meyers, Shari, Sun, Wuhua, Shopland, Lindsay, ...
Transcription factors of the AML (core binding factor-α/polyoma enhancer binding protein 2) class are key transactivators of tissue-specific genes of the hematopoietic and bone lineages. Alternative...
Hou, Zhen, Nguyen, Que, Frenkel, Baruch, Nilsson, Susan K., Milne, Moira, Van Wijnen, André J., ...
Somatic gene therapies require targeted transfer of the therapeutic gene(s) into stem cells that proliferate and then differentiate and express the gene in a tissue-restricted manner. We have...
McNeil, Sandra, Zeng, Congmei, Harrington, Kimberly S., Hiebert, Scott, Lian, Jane B., Stein, Janet L., ...
Targeting of gene regulatory factors to specific intranuclear sites may be critical for the accurate control of gene expression. The acute myelogenous leukemia 8;21 (AML1/ETO) fusion protein is...
Shopland, Lindsay S., Byron, Meg, Stein, Janet L., Lian, Jane B., Stein, Gary S., Lawrence, Jeanne B.
Interactions between Cajal bodies (CBs) and replication-dependent histone loci occur more frequently than for other mRNA-encoding genes, but such interactions are not seen with all alleles at a given...
Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Zaidi, S. Kaleem, Xing, Lianping, Balint, Eva, ...
Runx (Cbfa/AML) transcription factors are critical for tissue-specific gene expression. A unique targeting signal in the C terminus directs Runx factors to discrete foci within the nucleus. Using...
Javed, Amjad, Gutierrez, Soraya, Montecino, Martin, Van Wijnen, André J., Stein, Janet L., Stein, Gary S., ...
Three Cbfa motifs are strategically positioned in the bone-specific rat osteocalcin (rOC) promoter. Sites A and B flank the vitamin D response element in the distal promoter and sites B and C flank a...
Javed, Amjad, Barnes, George L., Jasanya, B. O., Stein, Janet L., Gerstenfeld, Louis, Lian, Jane B., ...
Expression of the bone sialoprotein (BSP) gene, a marker of bone formation, is largely restricted to cells in mineralized tissues. Recent studies have shown that the Cbfa1 (also known as Runx2,...
Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites
Zaidi, Sayyed K., Sullivan, Andrew J., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.
Runx factors control lineage commitment and are transcriptional effectors of Smad signaling. Genetic defects in these pathways interfere with normal development. The in situ localization of Runx and...
Luong, Mai X., Van Der Meijden, Caroline M., Xing, DongXia, Hesselton, Ruth, Monuki, Edwin S., Jones, Stephen N., ...
Murine CDP/Cux, a homologue of the Drosophila Cut homeoprotein, modulates the promoter activity of cell cycle-related and cell-type-specific genes. CDP/Cux interacts with histone gene promoters as...
Multiple subnuclear targeting signals of the leukemia-related AML1/ETO and ETO repressor proteins
Barseguian, Karina, Lutterbach, Bart, Hiebert, Scott W., Nickerson, Jeffrey, Lian, Jane B., Stein, Janet L., ...
Leukemic disease can be linked to aberrant gene expression. This often is the result of molecular alterations in transcription factors that lead to their misrouting within the nucleus. The acute...
Hovhannisyan, Hayk, Cho, Brian, Mitra, Partha, Montecino, Martin, Stein, Gary S., Van Wijnen, Andre J., ...
During the shutdown of proliferation and onset of differentiation of HL-60 promyelocytic leukemia cells, expression of the cell cycle-dependent histone genes is downregulated at the level of...
Sierra, Jose, Villagra, Alejandro, Paredes, Roberto, Cruzat, Fernando, Gutierrez, Soraya, Javed, Amjad, ...
p300 is a multifunctional transcriptional coactivator that serves as an adapter for several transcription factors including nuclear steroid hormone receptors. p300 possesses an intrinsic histone...
Mitra, Partha, Xie, Rong-Lin, Medina, Ricardo, Hovhannisyan, Hayk, Zaidi, S. Kaleem, Wei, Yue, ...
At the G1/S phase cell cycle transition, multiple histone genes are expressed to ensure that newly synthesized DNA is immediately packaged as chromatin. Here we have purified and functionally...
Zaidi, Sayyed K., Young, Daniel W., Pockwinse, Shirwin M., Javed, Amjad, Lian, Jane B., Stein, Janet L., ...
Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately...
Zaidi, Sayyed K, Sullivan, Andrew J, Medina, Ricardo, Ito, Yoshiaki, Van Wijnen, Andre J, Stein, Janet L, ...
Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of...
Hassan, Mohammad Q., Javed, Amjad, Morasso, Maria I., Karlin, Jeremy, Montecino, Martin, Van Wijnen, Andre J., ...
Genetic studies show that Msx2 and Dlx5 homeodomain (HD) proteins support skeletal development, but null mutation of the closely related Dlx3 gene results in early embryonic lethality. Here we find...
Paredes, Roberto, Arriagada, Gloria, Cruzat, Fernando, Villagra, Alejandro, Olate, Juan, Zaidi, Kaleem, ...
Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1α,25-dihydroxyvitamin D3 via its...
Javed, Amjad, Barnes, George L., Pratap, Jitesh, Antkowiak, Tomasz, Gerstenfeld, Louis C., Van Wijnen, Andre J., ...
Runx transcription factors comprise a family of proteins that are essential for organogenesis. A unique nuclear matrix-targeting signal in the C terminus directs these factors to their appropriate...
Vradii, Diana, Zaidi, Sayyed K., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.
The multifunctional C terminus of the hematopoietic AML1 transcription factor interacts with coregulatory proteins, supports the convergence and integration of physiological signals, and contains the...
Miele, Angela, Braastad, Corey D., Holmes, William F., Mitra, Partha, Medina, Ricardo, Xie, Ronglin, ...
Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the...
Lederman, Sally Ann, Rauh, Virginia, Weiss, Lisa, Stein, Janet L., Hoepner, Lori A., Becker, Mark, ...
The effects of prenatal exposure to pollutants from the World Trade Center (WTC) disaster on fetal growth and subsequent health and development of exposed children remain a source of concern. We...
Pratap, Jitesh, Javed, Amjad, Languino, Lucia R., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., ...
The Runx2 (Cbfa1/AML3) transcription factor and matrix metalloproteinase 9 (MMP9) are key regulators of growth plate maturation and bone formation. The genes for both proteins are characteristic...
The dynamic organization of gene-regulatory machinery in nuclear microenvironments
Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin, ...
Nuclear components are functionally linked with the dynamic temporal and spatial compartmentalization, sorting and integration of regulatory information to facilitate its selective use. For example,...
Young, Daniel W., Hassan, Mohammad Q., Yang, Xiao-Qing, Galindo, Mario, Javed, Amjad, Zaidi, Sayyed K., ...
During cell division, cessation of transcription is coupled with mitotic chromosome condensation. A fundamental biological question is how gene expression patterns are retained during mitosis to...
HOXA10 Controls Osteoblastogenesis by Directly Activating Bone Regulatory and Phenotypic Genes▿
Hassan, Mohammad Q., Tare, Rahul, Lee, Suk Hee, Mandeville, Matthew, Weiner, Brian, Montecino, Martin, ...
HOXA10 is necessary for embryonic patterning of skeletal elements, but its function in bone formation beyond this early developmental stage is unknown. Here we show that HOXA10 contributes to...
Perera, Frederica P., Tang, Deliang, Rauh, Virginia, Tu, Yi Hsuan, Tsai, Wei Yann, Becker, Mark, ...
Zaidi, Sayyed K., Pande, Sandhya, Pratap, Jitesh, Gaur, Tripti, Grigoriu, Simina, Ali, Syed A., ...
The osteogenic Runt-related (Runx2) transcription factor negatively regulates proliferation and ribosomal gene expression in normal diploid osteoblasts, but is up-regulated in metastatic breast and...
Lederman, Sally Ann, Jones, Robert L., Caldwell, Kathleen L., Rauh, Virginia, Sheets, Stephen E., Tang, Deliang, ...
Phenotypic transcription factors epigenetically mediate cell growth control
Ali, Syed A., Zaidi, Sayyed K., Dacwag, Caroline S., Salma, Nunciada, Young, Daniel W., Shakoori, Abdul R., ...
Ribosomal RNA (rRNA) genes are down-regulated during osteogenesis, myogenesis, and adipogenesis, necessitating a mechanistic understanding of interrelationships between growth control and phenotype...
A microRNA signature for a BMP2-induced osteoblast lineage commitment program
Li, Zhaoyong, Hassan, Mohammad Q., Volinia, Stefano, Van Wijnen, Andre J., Stein, Janet L., Croce, Carlo M., ...
Bone morphogenetic proteins (BMPs) are potent morphogens that activate transcriptional programs for lineage determination. How BMP induction of a phenotype is coordinated with microRNAs (miRNAs) that...
Structural Coupling of Smad and Runx2 for Execution of the BMP2 Osteogenic Signal*
Javed, Amjad, Bae, Jong-Sup, Afzal, Faiza, Gutierrez, Soraya, Pratap, Jitesh, Zaidi, Sayyed K., ...
Two regulatory pathways, bone morphogenetic protein (BMP)/transforming growth factor-β (TGFβ) and the transcription factor RUNX2, are required for bone formation in vivo. Here we show the...
Ghule, Prachi N., Dominski, Zbigniew, Yang, Xiao-cui, Marzluff, William F., Becker, Klaus A., Harper, J. Wade, ...
Human embryonic stem (hES) cells have an abbreviated G1 phase of the cell cycle. How cells expedite G1 events that are required for the initiation of S phase has not been resolved. One key regulatory...
Runx2 Regulates G Protein-coupled Signaling Pathways to Control Growth of Osteoblast Progenitors*
Teplyuk, Nadiya M., Galindo, Mario, Teplyuk, Viktor I., Pratap, Jitesh, Young, Daniel W., Lapointe, David, ...
Runt-related transcription factor 2 (Runx2) controls lineage commitment, proliferation, and anabolic functions of osteoblasts as the subnuclear effector of multiple signaling axes (e.g. transforming...