Jason E. Pike

Pif1 Helicase Lengthens Some Okazaki Fragment Flaps Necessitating Dna2 Nuclease/Helicase Action in the Two-nuclease Processing Pathway (2009)

Pike, Jason E., Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.

We have developed a system to reconstitute all of the proposed steps of Okazaki fragment processing using purified yeast proteins and model substrates. DNA polymerase δ was shown to extend an...

Pif1 helicase directs eukaryotic Okazaki fragments toward the two-nuclease cleavage pathway for primer removal (2008)

Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.

Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase delta (Pol delta) extends the upstream Okazaki fragment and displaces...

Pif1 helicase directs eukaryotic Okazaki fragments toward the two-nuclease cleavage pathway for primer removal (2008)

Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.

Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase delta (Pol delta) extends the upstream Okazaki fragment and displaces...

Pif1 Helicase Directs Eukaryotic Okazaki Fragments toward the Two-nuclease Cleavage Pathway for Primer Removal*

Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.

Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase δ (Pol δ) extends the upstream Okazaki fragment and displaces the...