Jean-Marc Costa

Uniform distribution of three Candida albicansmicrosatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination (2006)

Eloy, Odile, Marque, Stéphanie, Botterel, Françoise, Stephan, François, Costa, Jean-Marc, Lasserre, Virginie, ...

Abstract Background The nosocomial acquisition of Candida albicans is a growing concern in intensive care units (ICUs) and understanding the route of contamination is relevant for infection control...

Very early prenatal diagnosis of genetic diseases based on coelomic fluid analysis: a feasibility study (2006)

Jouannic, Jean-Marie, Costa, Jean-Marc, Ernault, Pauline, Bénifla, Jean-Louis

BACKGROUND: Coelocentesis may represent the ideal technique for very early prenatal diagnosis. Although cell density in coelomic fluid (CF) is very low, only techniques performed on this cellular...

Very early prenatal diagnosis of genetic diseases based on coelomic fluid analysis: a feasibility study (2006)

Jouannic, Jean-Marie, Costa, Jean-Marc, Ernault, Pauline, Bénifla, Jean-Louis

BACKGROUND: Coelocentesis may represent the ideal technique for very early prenatal diagnosis. Although cell density in coelomic fluid (CF) is very low, the results of analyses on the cellular...

Very early prenatal diagnosis of genetic diseases based on coelomic fluid analysis: a feasibility study (2006)

Jouannic, Jean-Marie, Costa, Jean-Marc, Ernault, Pauline, Bénifla, Jean-Louis

BACKGROUND: Coelocentesis may represent the ideal technique for very early prenatal diagnosis. Although cell density in coelomic fluid (CF) is very low, only techniques performed on this cellular...

Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes (2003)

Reischl, Udo, Bretagne, Stéphane, Krüger, Dominique, Ernault, Pauline, Costa, Jean-Marc

Abstract Background Toxoplasmosis is an infectious disease caused by the parasitic protozoan Toxoplasma gondii. It is endemic worldwide and, depending on the geographic location, 15 to 85% of the...

Clinical applications of fetal sex determination in maternal blood in a preimplantation genetic diagnosis centre (2002)

Tachdjian, Gérard, Frydman, Nelly, Audibert, Franciois, Ray, Pierre, Kerbrat, Violaine, Ernault, Pauline, ...

BACKGROUND: Couples with a risk of transmitting X-linked diseases who are included in a preimplantation genetic diagnosis (PGD) programme need early and rapid fetal sex determination in two...

Real-Time PCR Coupled with Automated DNA Extraction and Detection of Galactomannan Antigen in Serum by Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis

Costa, Catherine, Costa, Jean-Marc, Desterke, Christophe, Botterel, Françoise, Cordonnier, Catherine, Bretagne, Stéphane

To improve the diagnosis of invasive aspergillosis (IA), we developed a LightCycler PCR assay targeted to Aspergillus fumigatus and A. flavus mitochondrial DNA. To avoid contamination, fully...

Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes

Reischl, Udo, Bretagne, Stéphane, Krüger, Dominique, Ernault, Pauline, Costa, Jean-Marc

Fast and Accurate Quantitative Detection of Helicobacter pylori and Identification of Clarithromycin Resistance Mutations in H. pylori Isolates from Gastric Biopsy Specimens by Real-Time PCR

Lascols, Christine, Lamarque, Dominique, Costa, Jean-Marc, Copie-Bergman, Christiane, Le Glaunec, Jeanne-Marie, Deforges, Lionel, ...

Rapid identification of patients infected with clarithromycin-resistant Helicobacter pylori without the need for culture can help to avoid useless prescriptions of clarithromycin. We developed and...

Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

Picone, Olivier, Costa, Jean-Marc, Chaix, Marie-Laure, Ville, Yves, Rouzioux, Christine, Leruez-Ville, Marianne

The human cytomegalovirus (HCMV) UL144 gene is a tumor necrosis factor-like receptor with the potential to affect HCMV virulence. HCMV strains display genetic variability in the UL144 region, and the...

Use of Real-Time PCR To Process the First Galactomannan-Positive Serum Sample in Diagnosing Invasive Aspergillosis

Millon, Laurence, Piarroux, Renaud, Deconinck, Eric, Bulabois, Claude-Eric, Grenouillet, Frédéric, Rohrlich, Pierre, ...

Positive galactomannan (GM) antigenemias are included as a microbiological item in the diagnosis of probable or possible invasive aspergillosis (IA). Because false-positive GM results frequently...

Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination

Eloy, Odile, Marque, Stéphanie, Botterel, Françoise, Stephan, François, Costa, Jean-Marc, Lasserre, Virginie, ...

Real-Time PCR Coupled with Automated DNA Extraction and Detection of Galactomannan Antigen in Serum by Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis

Costa, Catherine, Costa, Jean-Marc, Desterke, Christophe, Botterel, Françoise, Cordonnier, Catherine, Bretagne, Stéphane

To improve the diagnosis of invasive aspergillosis (IA), we developed a LightCycler PCR assay targeted to Aspergillus fumigatus and A. flavus mitochondrial DNA. To avoid contamination, fully...

Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes

Reischl, Udo, Bretagne, Stéphane, Krüger, Dominique, Ernault, Pauline, Costa, Jean-Marc

Fast and Accurate Quantitative Detection of Helicobacter pylori and Identification of Clarithromycin Resistance Mutations in H. pylori Isolates from Gastric Biopsy Specimens by Real-Time PCR

Lascols, Christine, Lamarque, Dominique, Costa, Jean-Marc, Copie-Bergman, Christiane, Le Glaunec, Jeanne-Marie, Deforges, Lionel, ...

Rapid identification of patients infected with clarithromycin-resistant Helicobacter pylori without the need for culture can help to avoid useless prescriptions of clarithromycin. We developed and...

Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

Picone, Olivier, Costa, Jean-Marc, Chaix, Marie-Laure, Ville, Yves, Rouzioux, Christine, Leruez-Ville, Marianne

The human cytomegalovirus (HCMV) UL144 gene is a tumor necrosis factor-like receptor with the potential to affect HCMV virulence. HCMV strains display genetic variability in the UL144 region, and the...

Use of Real-Time PCR To Process the First Galactomannan-Positive Serum Sample in Diagnosing Invasive Aspergillosis

Millon, Laurence, Piarroux, Renaud, Deconinck, Eric, Bulabois, Claude-Eric, Grenouillet, Frédéric, Rohrlich, Pierre, ...

Positive galactomannan (GM) antigenemias are included as a microbiological item in the diagnosis of probable or possible invasive aspergillosis (IA). Because false-positive GM results frequently...

Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination

Eloy, Odile, Marque, Stéphanie, Botterel, Françoise, Stephan, François, Costa, Jean-Marc, Lasserre, Virginie, ...

Detection and Identification of Leishmania Species from Clinical Specimens by Using a Real-Time PCR Assay and Sequencing of the Cytochrome b Gene▿

Foulet, Françoise, Botterel, Françoise, Buffet, Pierre, Morizot, Gloria, Rivollet, Danièle, Deniau, Michèle, ...

Visceral and cutaneous leishmaniases are heterogenous entities. The Leishmania species that a given patient harbors usually cannot be determined clinically, and this identification is essential to...

Comparison of Microsatellite Length Polymorphism and Multilocus Sequence Typing for DNA-Based Typing of Candida albicans▿

Garcia-Hermoso, Dea, Cabaret, Odile, Lecellier, Gael, Desnos-Ollivier, Marie, Hoinard, Damien, Raoux, Dorothée, ...

For genotyping Candida albicans isolates, two PCR-based methods have recently emerged: multilocus sequence typing (MLST), based on the sequence of selected genes, and microsatellite length...