Scientific Commons: Jean Content

Characterization of Human Mycobacterium bovis Bacille Calmette-Guérin-Reactive CD8+ T Cells

Smith, Steven M., Malin, Adam S., Pauline T., Lukey, Atkinson, Sara E., Content, Jean, ...

Gamma interferon (IFN-γ)-secreting CD4+ T cells have long been established as an essential component of the protective immune response against Mycobacterium tuberculosis. It is now becoming evident...

Listeria monocytogenes Possesses Adhesins for Fibronectin

Gilot, Philippe, André, Paul, Content, Jean

Listeria monocytogenes is a gram-positive, nonsporulating, food-borne pathogen of humans and animals that is able to invade many eukaryotic cells. Several listerial surface components have been...

Cloning of the Gene Encoding a 22-Kilodalton Cell Surface Antigen of Mycobacterium bovis BCG and Analysis of Its Potential for DNA Vaccination against Tuberculosis

Lefèvre, Philippe, Denis, Olivier, De Wit, Lucas, Tanghe, Audrey, Vandenbussche, Paul, Content, Jean, ...

Using spleen cells from mice vaccinated with live Mycobacterium bovis BCG, we previously generated three monoclonal antibodies reactive against a 22-kDa protein present in mycobacterial culture...

Lack of Protection in Mice and Necrotizing Bronchointerstitial Pneumonia with Bronchiolitis in Guinea Pigs Immunized with Vaccines Directed against the hsp60 Molecule of Mycobacterium tuberculosis

Turner, Oliver C., Roberts, Alan D., Frank, Anthony A., Phalen, Susan W., McMurray, David M., Content, Jean, ...

In this study, the hsp60 and hsp70 heat shock protein antigens of Mycobacterium tuberculosis were tested as potential vaccine candidates, using purified recombinant protein antigens or antigens...

Protective Efficacy of a DNA Vaccine Encoding Antigen 85A from Mycobacterium bovis BCG against Buruli Ulcer

Tanghe, Audrey, Content, Jean, Van Vooren, Jean-Paul, Portaels, Françoise, Huygen, Kris

Buruli ulcer, caused by Mycobacterium ulcerans, is characterized by deep and necrotizing skin lesions, mostly on the arms and legs. Together with tuberculosis and leprosy, this mycobacterial disease...

Nomenclature of Genes Encoding Aminoglycoside-Modifying Enzymes

Vanhoof, Raymond, Hannecart-Pokorni, Eleonora, Content, Jean

Characterization of the 6′-N-Aminoglycoside Acetyltransferase Gene aac(6′)-Il Associated with a sulI-Type Integron

Hannecart-Pokorni, Eleonora, Depuydt, Freddy, Wit, Lucas De, Bossuyt, Eddie Van, Content, Jean, Vanhoof, Raymond

Vaccination with Plasmid DNA Encoding Mycobacterial Antigen 85A Stimulates a CD4+ and CD8+ T-Cell Epitopic Repertoire Broader than That Stimulated by Mycobacterium tuberculosis H37Rv Infection

Denis, Olivier, Tanghe, Audrey, Palfliet, Kamiel, Jurion, Fabienne, Van Den Berg, Thierry-P., Vanonckelen, Albert, ...

Vaccination of mice with plasmid DNA carrying the gene for the major secreted mycobacterial antigen 85A (Ag85A) from Mycobacterium tuberculosis is a powerful technique for generating robust specific...

Specific Identification of Listeria welshimeri and Listeria monocytogenes by PCR Assays Targeting a Gene Encoding a Fibronectin-Binding Protein

Gilot, Philippe, Content, Jean

We have cloned and sequenced a Listeria welshimeri DNA fragment homologous to the previously described fibronectin-binding protein-encoding gene (fbp) of Listeria monocytogenes (P. Gilot, Y. Jossin,...

Structure of the Ribonucleoprotein of Influenza Virus

Compans, Richard W., Content, Jean, Duesberg, Peter H.

The ribonucleoprotein (RNP) internal components of influenza virus were separated into distinct size classes by sedimentation in glycerol gradients and examined by electron microscopy by using...

Electrophoretic Distribution of the Proteins and Glycoproteins of Influenza Virus and Sendai Virus

Content, Jean, Duesberg, Peter H.

The proteins of influenza (WSN) and Sendai virus have been separated by polyacrylamide gel electrophoresis into five components. In both cases, three of these components were shown to be...

Mycobacterium tuberculosis with Disruption in Genes Encoding the Phosphate Binding Proteins PstS1 and PstS2 Is Deficient in Phosphate Uptake and Demonstrates Reduced In Vivo Virulence

Peirs, Priska, Lefèvre, Philippe, Boarbi, Samira, Wang, Xiao-Ming, Denis, Olivier, Braibant, Martine, ...

By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding...

Characterization of a potent human interleukin-11 agonist.

Harmegnies, Dimitri, Wang, Xiao-Ming, Vandenbussche, Paul, Leon, Arnaud, Vusio, Patricia, Grötzinger, Joachim, ...

Human interleukin-11 (hIL-11) is a multi-potential cytokine that is involved in numerous biological activities, such as haematopoiesis, osteoclastogenesis, neurogenesis and female fertility, and also...