An experimentally derived confidence score for binary protein-protein interactions (2009)
Braun, Pascal, Tasan, Murat, Dreze, Matija, Barrios-Rodiles, Miriam, Lemmens, Irma, Yu, Haiyuan, ...
Information on protein-protein interactions is of central importance for many areas of biomedical research. At present no method exists to systematically and experimentally assess the quality of...
Simonis, Nicolas, Rual, Jean-Francois, Carvunis, Anne-Ruxandra, Tasan, Murat, Lemmens, Irma, Hirozane-Kishikawa, Tomoko, ...
Abstract: To provide accurate biological hypotheses and elucidate global properties of cellular networks, systematic identification of protein-protein interactions must meet high quality standards....
Lamesch, Philippe, Milstein, Stuart, Hao, Tong, Rosenberg, Jennifer, Li, Ning, Sequerra, Reynaldo, ...
The first version of the Caenorhabditis elegans ORFeome cloning project, based on release WS9 of Wormbase (August 1999), provided experimental verifications for ∼55% of predicted protein-encoding...
Human ORFeome Version 1.1: A Platform for Reverse Proteomics (2004)
Rual, Jean-François, Hirozane-Kishikawa, Tomoko, Hao, Tong, Bertin, Nicolas, Li, Siming, Dricot, Amélie, ...
The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs), or ORFeomes, to allow functional studies of the corresponding proteomes....
Toward Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based RNAi Library (2004)
Rual, Jean-François, Ceron, Julian, Koreth, John, Hao, Tong, Nicot, Anne-Sophie, Hirozane-Kishikawa, Tomoko, ...
The recently completed Caenorhabditis elegans genome sequence allows application of high-throughput (HT) approaches for phenotypic analyses using RNA interference (RNAi). As large phenotypic data...
Generation of the Brucella melitensis ORFeome Version 1.1 (2004)
Dricot, Amélie, Rual, Jean-François, Lamesch, Philippe, Bertin, Nicolas, Dupuy, Denis, Hao, Tong, ...
The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the α-proteobacteria group, as many other bacteria that live in close association with...
Antiglucocorticoid activity of hepatocyte nuclear factor-6
Pierreux, Christophe E., Stafford, John, Demonte, Dominique, Scott, Donald K., Vandenhaute, Jean, O’Brien, Richard M., ...
Glucocorticoids exert their effects on gene transcription through ubiquitous receptors that bind to regulatory sequences present in many genes. These glucocorticoid receptors are present in all cell...
Specificity of Cdk activation in vivo by the two Caks Mcs6 and Csk1 in fission yeast
Hermand, Damien, Westerling, Thomas, Pihlak, Arno, Thuret, Jean-Yves, Vallenius, Tea, Tiainen, Marianne, ...
Activating phosphorylation of cyclin-dependent kinases (Cdks) is mediated by at least two structurally distinct types of Cdk-activating kinases (Caks): the trimeric Cdk7–cyclin H–Mat1 complex in...
Charbon, Godefroid, Breunig, Karin D., Wattiez, Ruddy, Vandenhaute, Jean, Noël-Georis, Isabelle
Utilization of nonfermentable carbon sources by Kluyveromyces lactis and Saccharomyces cerevisiae requires the Snf1p kinase and the Cat8p transcriptional activator, which binds to carbon...
Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS
Wery, Maxime, Shematorova, Elena, Van Driessche, Benoît, Vandenhaute, Jean, Thuriaux, Pierre, Van Mullem, Vincent
TFIIS, an elongation factor encoded by DST1 in Saccharomyces cerevisiae, stimulates transcript cleavage in arrested RNA polymerase II. Two components of the RNA polymerase II machinery, Med13 (Srb9)...
Lamesch, Philippe, Milstein, Stuart, Hao, Tong, Rosenberg, Jennifer, Li, Ning, Sequerra, Reynaldo, ...
The first version of the Caenorhabditis elegans ORFeome cloning project, based on release WS9 of Wormbase (August 1999), provided experimental verifications for ∼55% of predicted protein-encoding...
Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Rual, Jean-François, Hirozane-Kishikawa, Tomoko, Hao, Tong, Bertin, Nicolas, Li, Siming, Dricot, Amélie, ...
The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs), or ORFeomes, to allow functional studies of the corresponding proteomes....
Toward Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based RNAi Library
Rual, Jean-François, Ceron, Julian, Koreth, John, Hao, Tong, Nicot, Anne-Sophie, Hirozane-Kishikawa, Tomoko, ...
The recently completed Caenorhabditis elegans genome sequence allows application of high-throughput (HT) approaches for phenotypic analyses using RNA interference (RNAi). As large phenotypic data...
Generation of the Brucella melitensis ORFeome Version 1.1
Dricot, Amélie, Rual, Jean-François, Lamesch, Philippe, Bertin, Nicolas, Dupuy, Denis, Hao, Tong, ...
The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the α-proteobacteria group, as many other bacteria that live in close association with...
Antiglucocorticoid activity of hepatocyte nuclear factor-6
Pierreux, Christophe E., Stafford, John, Demonte, Dominique, Scott, Donald K., Vandenhaute, Jean, O’Brien, Richard M., ...
Glucocorticoids exert their effects on gene transcription through ubiquitous receptors that bind to regulatory sequences present in many genes. These glucocorticoid receptors are present in all cell...
Specificity of Cdk activation in vivo by the two Caks Mcs6 and Csk1 in fission yeast
Hermand, Damien, Westerling, Thomas, Pihlak, Arno, Thuret, Jean-Yves, Vallenius, Tea, Tiainen, Marianne, ...
Activating phosphorylation of cyclin-dependent kinases (Cdks) is mediated by at least two structurally distinct types of Cdk-activating kinases (Caks): the trimeric Cdk7–cyclin H–Mat1 complex in...
Charbon, Godefroid, Breunig, Karin D., Wattiez, Ruddy, Vandenhaute, Jean, Noël-Georis, Isabelle
Utilization of nonfermentable carbon sources by Kluyveromyces lactis and Saccharomyces cerevisiae requires the Snf1p kinase and the Cat8p transcriptional activator, which binds to carbon...
Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS
Wery, Maxime, Shematorova, Elena, Van Driessche, Benoît, Vandenhaute, Jean, Thuriaux, Pierre, Van Mullem, Vincent
TFIIS, an elongation factor encoded by DST1 in Saccharomyces cerevisiae, stimulates transcript cleavage in arrested RNA polymerase II. Two components of the RNA polymerase II machinery, Med13 (Srb9)...
Lamesch, Philippe, Milstein, Stuart, Hao, Tong, Rosenberg, Jennifer, Li, Ning, Sequerra, Reynaldo, ...
The first version of the Caenorhabditis elegans ORFeome cloning project, based on release WS9 of Wormbase (August 1999), provided experimental verifications for ∼55% of predicted protein-encoding...
Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Rual, Jean-François, Hirozane-Kishikawa, Tomoko, Hao, Tong, Bertin, Nicolas, Li, Siming, Dricot, Amélie, ...
The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs), or ORFeomes, to allow functional studies of the corresponding proteomes....
Toward Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based RNAi Library
Rual, Jean-François, Ceron, Julian, Koreth, John, Hao, Tong, Nicot, Anne-Sophie, Hirozane-Kishikawa, Tomoko, ...
The recently completed Caenorhabditis elegans genome sequence allows application of high-throughput (HT) approaches for phenotypic analyses using RNA interference (RNAi). As large phenotypic data...
Generation of the Brucella melitensis ORFeome Version 1.1
Dricot, Amélie, Rual, Jean-François, Lamesch, Philippe, Bertin, Nicolas, Dupuy, Denis, Hao, Tong, ...
The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the α-proteobacteria group, as many other bacteria that live in close association with...
Hallez, Régis, Letesson, Jean-Jacques, Vandenhaute, Jean, De Bolle, Xavier
Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful...
Tafforeau, Lionel, Le Blastier, Sophie, Bamps, Sophie, Dewez, Monique, Vandenhaute, Jean, Hermand, Damien
We describe a new member of the F-box family, Pof14, which forms a canonical, F-box dependent SCF (Skp1, Cullin, F-box protein) ubiquitin ligase complex. The Pof14 protein has intrinsic instability...
Hallez, Régis, Mignolet, Johann, Van Mullem, Vincent, Wery, Maxime, Vandenhaute, Jean, Letesson, Jean-Jacques, ...
Many organisms use polar localization of signalling proteins to control developmental events in response to completion of asymmetric cell division. Asymmetric division was recently reported for...
Recruitment of P-TEFb (Cdk9-Pch1) to chromatin by the cap-methyl transferase Pcm1 in fission yeast
Guiguen, Allan, Soutourina, Julie, Dewez, Monique, Tafforeau, Lionel, Dieu, Marc, Raes, Martine, ...
Capping of nascent pre-mRNAs is thought to be a prerequisite for productive elongation and associated serine 2 phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (PolII). The...
The conserved Wobble uridine tRNA thiolase Ctu1–Ctu2 is required to maintain genome integrity
Dewez, Monique, Bauer, Fanélie, Dieu, Marc, Raes, Martine, Vandenhaute, Jean, Hermand, Damien
Modified nucleosides close to the anticodon are important for the proper decoding of mRNA by the ribosome. Particularly, the uridine at the first anticodon position (U34) of glutamate, lysine, and...