Jeffery L. Dangl

A draft genome sequence and functional screen reveals the repertoire of type III secreted proteins of Pseudomonas syringaepathovar tabaci11528 (2009)

Studholme, David J, Ibanez, Selena, MacLean, Daniel, Dangl, Jeffery L, Chang, Jeff H, Rathjen, John P

Abstract Background Pseudomonas syringae is a widespread bacterial pathogen that causes disease on a broad range of economically important plant species. Pathogenicity of P. syringae strains is...

De novo assembly using low-coverage short read sequence data from the rice pathogen Pseudomonas syringae pv. oryzae (2009)

Reinhardt, Josephine A., Baltrus, David A., Nishimura, Marc T., Jeck, William R., Jones, Corbin D., Dangl, Jeffery L.

We developed a novel approach for de novo genome assembly using only sequence data from high-throughput short read sequencing technologies. By combining data generated from 454 Life Sciences (Roche)...

Autoimmune Response as a Mechanism for a Dobzhansky-Muller-Type Incompatibility Syndrome in Plants (2007)

Kirsten Bomblies, Janne Lempe, Petra Epple, Norman Warthmann, Christa Lanz, Jeffery L. Dangl, ...

Epistatic interactions between genes are a major factor in evolution. Hybrid necrosis is an example of a deleterious phenotype caused by epistatic interactions that is observed in many intra- and...

Type III Effector Activation via Nucleotide Binding, Phosphorylation, and Host Target Interaction (2007)

Darrell Desveaux, Alex U. Singer, Ai-Jiuan Wu, Brian C. McNulty, Laura Musselwhite, Zachary Nimchuk, ...

The Pseudomonas syringae type III effector protein avirulence protein B (AvrB) is delivered into plant cells, where it targets the Arabidopsis RIN4 protein (resistance to Pseudomonas maculicula...

ArabidopsisWRKY70 is required for full RPP4-mediated disease resistance and basal defense against Hyaloperonospora parasitica (2007)

Knoth, Colleen, Ringler, Jon, Dangl, Jeffery L, Eulgem, Thomas

AtWRKY70, encoding a WRKY transcription factor, is co-expressed with a set of Arabidopsis genes that share a pattern of RPP4- and RPP7-dependent late upregulation in response to Hyaloperonospora...

Extending assembly of short DNA sequences to handle error (2007)

Jeck, William R., Reinhardt, Josephine A., Baltrus, David A., Hickenbotham, Matthew T., Magrini, Vincent, Mardis, Elaine R., ...

Inexpensive de novo genome sequencing, particularly in organisms with small genomes, is now possible using several new sequencing technologies. Some of these technologies such as that from Illumina's...

Independent deletions of a pathogen-resistance gene in Brassica and Arabidopsis

Grant, Murray R., McDowell, John M., Sharpe, Andrew G., De Torres Zabala, Marta, Lydiate, Derek J., Dangl, Jeffery L.

Plant disease resistance (R) genes confer race-specific resistance to pathogens and are genetically defined on the basis of intra-specific functional polymorphism. Little is known about the...

The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response

Boyes, Douglas C., Nam, Jaesung, Dangl, Jeffery L.

Disease resistance in plants is often controlled by a gene-for-gene mechanism in which avirulence (avr) gene products encoded by pathogens are specifically recognized, either directly or indirectly,...

Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response

Torres, Miguel Angel, Dangl, Jeffery L., Jones, Jonathan D. G.

Reactive oxygen intermediates (ROI) are strongly associated with plant defense responses. The origin of these ROI has been controversial. Arabidopsis respiratory burst oxidase homologues (rboh genes)...

The Disease Resistance Signaling Components EDS1 and PAD4 Are Essential Regulators of the Cell Death Pathway Controlled by LSD1 in Arabidopsis

Rustérucci, Christine, Aviv, Daniel H., Holt, Ben F., Dangl, Jeffery L., Parker, Jane E.

Specific recognition of pathogens is mediated by plant disease resistance (R) genes and translated into a successful defense response. The extent of associated hypersensitive cell death varies from...

Expression Profile Matrix of Arabidopsis Transcription Factor Genes Suggests Their Putative Functions in Response to Environmental StressesW⃞

Chen, Wenqiong, Provart, Nicholas J., Glazebrook, Jane, Katagiri, Fumiaki, Chang, Hur-Song, Eulgem, Thomas, ...

Numerous studies have shown that transcription factors are important in regulating plant responses to environmental stress. However, specific functions for most of the genes encoding transcription...

Arabidopsis SGT1b Is Required for Defense Signaling Conferred by Several Downy Mildew Resistance Genes

Tör, Mahmut, Gordon, Pam, Cuzick, Alayne, Eulgem, Thomas, Sinapidou, Evaggelia, Mert-Türk, Figen, ...

We describe the identification of a mutant in the Arabidopsis accession Columbia (Col-0) that exhibits enhanced downy mildew (edm1) susceptibility to several Peronospora parasitica isolates,...

RAR1 and NDR1 Contribute Quantitatively to Disease Resistance in Arabidopsis, and Their Relative Contributions Are Dependent on the R Gene Assayed

Tornero, Pablo, Merritt, Peter, Sadanandom, Ari, Shirasu, Ken, Innes, Roger W., Dangl, Jeffery L.

Plant disease resistance (R) genes mediate specific pathogen recognition, leading to a successful immune response. Downstream responses include ion fluxes, an oxidative burst, transcriptional...

Large-Scale Structure –Function Analysis of the Arabidopsis RPM1 Disease Resistance Protein

Tornero, Pablo, Chao, Ryon A., Luthin, William N., Goff, Stephen A., Dangl, Jeffery L.

The Arabidopsis RPM1 gene confers resistance against Pseudomonas syringae expressing either the AvrRpm1 or the AvrB type III effector protein. We present an exhaustive genetic screen for mutants that...

NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis

Kwak, June M., Mori, Izumi C., Pei, Zhen-Ming, Leonhardt, Nathalie, Torres, Miguel Angel, Dangl, Jeffery L., ...

Reactive oxygen species (ROS) have been proposed to function as second messengers in abscisic acid (ABA) signaling in guard cells. However, the question whether ROS production is indeed required for...

Antagonistic control of oxidative stress-induced cell death in Arabidopsis by two related, plant-specific zinc finger proteins

Epple, Petra, Mack, Amanda A., Morris, Veronica R. F., Dangl, Jeffery L.

The most familiar form of plant programmed cell death is the hypersensitive response (HR) associated with successful plant immune responses. HR is preceded by an oxidative burst and the generation of...

Cytosolic HSP90 associates with and modulates the Arabidopsis RPM1 disease resistance protein

Hubert, David A., Tornero, Pablo, Belkhadir, Youssef, Krishna, Priti, Takahashi, Akira, Shirasu, Ken, ...

The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We...

UV-inducible transient expression in parsley protoplasts identifies regulatory cis-elements of a chimeric Antirrhinum majus chalcone synthase gene

Lipphardt, Susanne, Brettschneider, Reinhold, Kreuzaler, Fritz, Schell, Jeff, Dangl, Jeffery L.

It was shown previously that parsley protoplasts retain differential responsiveness to external stimuli, e.g. UV light. This opened the way for the development of transient expression assays to...

Gene Expression Signatures from Three Genetically Separable Resistance Gene Signaling Pathways for Downy Mildew Resistance1[w]

Eulgem, Thomas, Weigman, Victor J., Chang, Hur-Song, McDowell, John M., Holub, Eric B., Glazebrook, Jane, ...

Resistance gene-dependent disease resistance to pathogenic microorganisms is mediated by genetically separable regulatory pathways. Using the GeneChip Arabidopsis genome array, we compared the...

A high-throughput, near-saturating screen for type III effector genes from Pseudomonas syringae

Chang, Jeff H., Urbach, Jonathan M., Law, Terry F., Arnold, Larry W., Hu, An, Gombar, Saurabh, ...

Pseudomonas syringae strains deliver variable numbers of type III effector proteins into plant cells during infection. These proteins are required for virulence, because strains incapable of...

Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor

Dangl, Jeffery L., Hauffe, Karl D., Lipphardt, Susanne, Hahlbrock, Klaus, Scheel, Dierk

The differential response of cultured parsley cells to u.v. irradiation and elicitor treatment is a paradigm for analysis of specific plant defense responses. We demonstrate that freshly isolated...

The Pseudomonas syringae effector AvrRpt2 cleaves its C-terminally acylated target, RIN4, from Arabidopsis membranes to block RPM1 activation

Kim, Han-Suk, Desveaux, Darrell, Singer, Alex U., Patel, Priyesh, Sondek, John, Dangl, Jeffery L.

Plant pathogenic Pseudomonas syringae deliver type III effector proteins into the host cell, where they function to manipulate host defense and metabolism to benefit the extracellular bacterial...

Isolation and characterization of broad-spectrum disease-resistant Arabidopsis mutants.

Maleck, Klaus, Neuenschwander, Urs, Cade, Rebecca M, Dietrich, Robert A, Dangl, Jeffery L, Ryals, John A

To identify Arabidopsis mutants that constitutively express systemic acquired resistance (SAR), we constructed reporter lines expressing the firefly luciferase gene under the control of the...

Diverse evolutionary mechanisms shape the type III effector virulence factor repertoire in the plant pathogen Pseudomonas syringae.

Rohmer, Laurence, Guttman, David S, Dangl, Jeffery L

Many gram-negative pathogenic bacteria directly translocate effector proteins into eukaryotic host cells via type III delivery systems. Type III effector proteins are determinants of virulence on...

Independent deletions of a pathogen-resistance gene in Brassica and Arabidopsis

Grant, Murray R., McDowell, John M., Sharpe, Andrew G., De Torres Zabala, Marta, Lydiate, Derek J., Dangl, Jeffery L.

Plant disease resistance (R) genes confer race-specific resistance to pathogens and are genetically defined on the basis of intra-specific functional polymorphism. Little is known about the...

The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response

Boyes, Douglas C., Nam, Jaesung, Dangl, Jeffery L.

Disease resistance in plants is often controlled by a gene-for-gene mechanism in which avirulence (avr) gene products encoded by pathogens are specifically recognized, either directly or indirectly,...

Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response

Torres, Miguel Angel, Dangl, Jeffery L., Jones, Jonathan D. G.

Reactive oxygen intermediates (ROI) are strongly associated with plant defense responses. The origin of these ROI has been controversial. Arabidopsis respiratory burst oxidase homologues (rboh genes)...

The Disease Resistance Signaling Components EDS1 and PAD4 Are Essential Regulators of the Cell Death Pathway Controlled by LSD1 in Arabidopsis

Rustérucci, Christine, Aviv, Daniel H., Holt, Ben F., Dangl, Jeffery L., Parker, Jane E.

Specific recognition of pathogens is mediated by plant disease resistance (R) genes and translated into a successful defense response. The extent of associated hypersensitive cell death varies from...

Expression Profile Matrix of Arabidopsis Transcription Factor Genes Suggests Their Putative Functions in Response to Environmental StressesW⃞

Chen, Wenqiong, Provart, Nicholas J., Glazebrook, Jane, Katagiri, Fumiaki, Chang, Hur-Song, Eulgem, Thomas, ...

Numerous studies have shown that transcription factors are important in regulating plant responses to environmental stress. However, specific functions for most of the genes encoding transcription...

Arabidopsis SGT1b Is Required for Defense Signaling Conferred by Several Downy Mildew Resistance Genes

Tör, Mahmut, Gordon, Pam, Cuzick, Alayne, Eulgem, Thomas, Sinapidou, Evaggelia, Mert-Türk, Figen, ...

We describe the identification of a mutant in the Arabidopsis accession Columbia (Col-0) that exhibits enhanced downy mildew (edm1) susceptibility to several Peronospora parasitica isolates,...

RAR1 and NDR1 Contribute Quantitatively to Disease Resistance in Arabidopsis, and Their Relative Contributions Are Dependent on the R Gene Assayed

Tornero, Pablo, Merritt, Peter, Sadanandom, Ari, Shirasu, Ken, Innes, Roger W., Dangl, Jeffery L.

Plant disease resistance (R) genes mediate specific pathogen recognition, leading to a successful immune response. Downstream responses include ion fluxes, an oxidative burst, transcriptional...

Large-Scale Structure –Function Analysis of the Arabidopsis RPM1 Disease Resistance Protein

Tornero, Pablo, Chao, Ryon A., Luthin, William N., Goff, Stephen A., Dangl, Jeffery L.

The Arabidopsis RPM1 gene confers resistance against Pseudomonas syringae expressing either the AvrRpm1 or the AvrB type III effector protein. We present an exhaustive genetic screen for mutants that...

NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis

Kwak, June M., Mori, Izumi C., Pei, Zhen-Ming, Leonhardt, Nathalie, Torres, Miguel Angel, Dangl, Jeffery L., ...

Reactive oxygen species (ROS) have been proposed to function as second messengers in abscisic acid (ABA) signaling in guard cells. However, the question whether ROS production is indeed required for...

Antagonistic control of oxidative stress-induced cell death in Arabidopsis by two related, plant-specific zinc finger proteins

Epple, Petra, Mack, Amanda A., Morris, Veronica R. F., Dangl, Jeffery L.

The most familiar form of plant programmed cell death is the hypersensitive response (HR) associated with successful plant immune responses. HR is preceded by an oxidative burst and the generation of...

Cytosolic HSP90 associates with and modulates the Arabidopsis RPM1 disease resistance protein

Hubert, David A., Tornero, Pablo, Belkhadir, Youssef, Krishna, Priti, Takahashi, Akira, Shirasu, Ken, ...

The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We...

UV-inducible transient expression in parsley protoplasts identifies regulatory cis-elements of a chimeric Antirrhinum majus chalcone synthase gene

Lipphardt, Susanne, Brettschneider, Reinhold, Kreuzaler, Fritz, Schell, Jeff, Dangl, Jeffery L.

It was shown previously that parsley protoplasts retain differential responsiveness to external stimuli, e.g. UV light. This opened the way for the development of transient expression assays to...

Gene Expression Signatures from Three Genetically Separable Resistance Gene Signaling Pathways for Downy Mildew Resistance1[w]

Eulgem, Thomas, Weigman, Victor J., Chang, Hur-Song, McDowell, John M., Holub, Eric B., Glazebrook, Jane, ...

Resistance gene-dependent disease resistance to pathogenic microorganisms is mediated by genetically separable regulatory pathways. Using the GeneChip Arabidopsis genome array, we compared the...

A high-throughput, near-saturating screen for type III effector genes from Pseudomonas syringae

Chang, Jeff H., Urbach, Jonathan M., Law, Terry F., Arnold, Larry W., Hu, An, Gombar, Saurabh, ...

Pseudomonas syringae strains deliver variable numbers of type III effector proteins into plant cells during infection. These proteins are required for virulence, because strains incapable of...

Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor

Dangl, Jeffery L., Hauffe, Karl D., Lipphardt, Susanne, Hahlbrock, Klaus, Scheel, Dierk

The differential response of cultured parsley cells to u.v. irradiation and elicitor treatment is a paradigm for analysis of specific plant defense responses. We demonstrate that freshly isolated...

The Pseudomonas syringae effector AvrRpt2 cleaves its C-terminally acylated target, RIN4, from Arabidopsis membranes to block RPM1 activation

Kim, Han-Suk, Desveaux, Darrell, Singer, Alex U., Patel, Priyesh, Sondek, John, Dangl, Jeffery L.

Plant pathogenic Pseudomonas syringae deliver type III effector proteins into the host cell, where they function to manipulate host defense and metabolism to benefit the extracellular bacterial...

Isolation and characterization of broad-spectrum disease-resistant Arabidopsis mutants.

Maleck, Klaus, Neuenschwander, Urs, Cade, Rebecca M, Dietrich, Robert A, Dangl, Jeffery L, Ryals, John A

To identify Arabidopsis mutants that constitutively express systemic acquired resistance (SAR), we constructed reporter lines expressing the firefly luciferase gene under the control of the...

Diverse evolutionary mechanisms shape the type III effector virulence factor repertoire in the plant pathogen Pseudomonas syringae.

Rohmer, Laurence, Guttman, David S, Dangl, Jeffery L

Many gram-negative pathogenic bacteria directly translocate effector proteins into eukaryotic host cells via type III delivery systems. Type III effector proteins are determinants of virulence on...

High-Throughput Sequencing of Arabidopsis microRNAs: Evidence for Frequent Birth and Death of MIRNA Genes

Fahlgren, Noah, Howell, Miya D., Kasschau, Kristin D., Chapman, Elisabeth J., Sullivan, Christopher M., Cumbie, Jason S., ...

In plants, microRNAs (miRNAs) comprise one of two classes of small RNAs that function primarily as negative regulators at the posttranscriptional level. Several MIRNA genes in the plant kingdom are...

Type III Effector Activation via Nucleotide Binding, Phosphorylation, and Host Target Interaction

Desveaux, Darrell, Singer, Alex U, Wu, Ai-Jiuan, McNulty, Brian C, Musselwhite, Laura, Nimchuk, Zachary, ...

The Pseudomonas syringae type III effector protein avirulence protein B (AvrB) is delivered into plant cells, where it targets the Arabidopsis RIN4 protein (resistance to Pseudomonas maculicula...

bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection

Kaminaka, Hironori, Näke, Christian, Epple, Petra, Dittgen, Jan, Schütze, Katia, Chaban, Christina, ...

Plants use sophisticated strategies to balance responses to oxidative stress. Programmed cell death, including the hypersensitive response (HR) associated with successful pathogen recognition, is one...

Autoimmune Response as a Mechanism for a Dobzhansky-Muller-Type Incompatibility Syndrome in Plants

Bomblies, Kirsten, Lempe, Janne, Epple, Petra, Warthmann, Norman, Lanz, Christa, Dangl, Jeffery L, ...

Epistatic interactions between genes are a major factor in evolution. Hybrid necrosis is an example of a deleterious phenotype caused by epistatic interactions that is observed in many intra- and...

Arabidopsis TAO1 is a TIR-NB-LRR protein that contributes to disease resistance induced by the Pseudomonas syringae effector AvrB

Eitas, Timothy K., Nimchuk, Zachary L., Dangl, Jeffery L.

The type III effector protein encoded by avirulence gene B (AvrB) is delivered into plant cells by pathogenic strains of Pseudomonas syringae. There, it localizes to the plasma membrane and triggers...

De novo assembly using low-coverage short read sequence data from the rice pathogen Pseudomonas syringae pv. oryzae

Reinhardt, Josephine A., Baltrus, David A., Nishimura, Marc T., Jeck, William R., Jones, Corbin D., Dangl, Jeffery L.

We developed a novel approach for de novo genome assembly using only sequence data from high-throughput short read sequencing technologies. By combining data generated from 454 Life Sciences (Roche)...

Specific Arabidopsis HSP90.2 alleles recapitulate RAR1 cochaperone function in plant NB-LRR disease resistance protein regulation

Hubert, David A., He, Yijian, McNulty, Brian C., Tornero, Pablo, Dangl, Jeffery L.

Both plants and animals require the activity of proteins containing nucleotide binding (NB) domain and leucine-rich repeat (LRR) domains for proper immune system function. NB-LRR proteins in plants...