Virus-mediated mRNA decay by hyperadenylation (2009)
Sokoloski, Kevin J, Chaskey, Emily L, Wilusz, Jeffrey
Abstract Degradation of cellular mRNAs during Kaposi's sarcoma-associated herpesvirus infection is associated with hyperadenylation of transcripts and a relocalization of cytoplasmic poly(A)-binding...
Aedes aegyptiuses RNA interference in defense against Sindbis virus infection (2008)
Campbell, Corey L, Keene, Kimberly M, Brackney, Douglas E, Olson, Ken E, Blair, Carol D, Wilusz, Jeffrey, ...
Abstract Background RNA interference (RNAi) is an important anti-viral defense mechanism. The Aedes aegypti genome encodes RNAi component orthologs, however, most populations of this mosquito are...
CUG-BP and 3'UTR sequences influence PARN-mediated deadenylation in mammalian cell extracts (2007)
Moraes,Karen C.M., Wilusz,Carol J., Wilusz,Jeffrey
Several mRNAs have been shown to exhibit distinct patterns of poly(A) shortening prior to their decay in vivo. In this study, we show that individual transcripts also demonstrate distinct patterns of...
Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome (2006)
Anderson, John R., Mukherjee, Devi, Muthukumaraswamy, Karthika, Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
We have previously demonstrated that PM-Scl-75, a component of the human exosome complex involved in RNA maturation and mRNA decay, can specifically interact with RNAs containing an AU-rich...
CUG-BP binds to RNA substrates and recruits PARN deadenylase (2006)
Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
CUG-BP is the human homolog of the Xenopus EDEN-BP, which was shown previously to bind to mRNAs, such as c-mos, that exhibit rapid deadenylation following fertilization of the oocyte. While several...
CUG-BP binds to RNA substrates and recruits PARN deadenylase (2006)
Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
CUG-BP is the human homolog of the Xenopus EDEN-BP, which was shown previously to bind to mRNAs, such as c-mos, that exhibit rapid deadenylation following fertilization of the oocyte. While several...
Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome (2006)
Anderson, John R., Mukherjee, Devi, Muthukumaraswamy, Karthika, Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
We have previously demonstrated that PM-Scl-75, a component of the human exosome complex involved in RNA maturation and mRNA decay, can specifically interact with RNAs containing an AU-rich...
Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome (2006)
Anderson, John R., Mukherjee, Devi, Muthukumaraswamy, Karthika, Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
We have previously demonstrated that PM-Scl-75, a component of the human exosome complex involved in RNA maturation and mRNA decay, can specifically interact with RNAs containing an AU-rich...
CUG-BP binds to RNA substrates and recruits PARN deadenylase (2006)
Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
CUG-BP is the human homolog of the Xenopus EDEN-BP, which was shown previously to bind to mRNAs, such as c-mos, that exhibit rapid deadenylation following fertilization of the oocyte. While several...
HU, JUN, LUTZ, CAROL S., WILUSZ, JEFFREY, TIAN, BIN
Polyadenylation is an essential step for the maturation of almost all cellular mRNAs in eukaryotes. In human cells, most poly(A) sites are flanked by the upstream AAUAAA hexamer or a close variant,...
Seal, Ruth, Temperley, Richard, Wilusz, Jeffrey, Lightowlers, Robert N.
PARN, a poly(A)-specific ribonuclease, binds the 5′ cap-structure of mRNA and initiates deadenylation-dependent decay. Eukaryotic initiation factor 4E (eIF4E) also binds to the cap structure, an...
HU, JUN, LUTZ, CAROL S., WILUSZ, JEFFREY, TIAN, BIN
Polyadenylation is an essential step for the maturation of almost all cellular mRNAs in eukaryotes. In human cells, most poly(A) sites are flanked by the upstream AAUAAA hexamer or a close variant,...
Sachchithananthan, Mythily, Stasinopoulos, Stan J., Wilusz, Jeffrey, Medcalf, Robert L.
The human prothrombin G20210A polymorphism located at the 3′ cleavage site of the mRNA results in elevated plasma prothrombin levels and increased risk of venous thrombosis. This polymorphism has...
Arhin, George K., Boots, Monika, Bagga, Paramjeet S., Milcarek, Christine, Wilusz, Jeffrey
Auxiliary factors likely play an important role in determining the polyadenylation efficiency of mammalian pre-mRNAs. We previously identified an auxiliary factor, hnRNP H/H′, which stimulates...
Milone, Joseph, Wilusz, Jeffrey, Bellofatto, Vivian
mRNA turnover is a regulated process that contributes to the steady state level of cytoplasmic mRNA. The amount of each mRNA determines, to a large extent, the amount of protein produced by that...
Bagga, Paramjeet S., Ford, Lance P., Chen, Fan, Wilusz, Jeffrey
A downstream G-rich sequence (GRS), GGGGGAGGU-GUGGG, has been previously shown to influence the efficiency of 3′ end processing of the SV40 late polyadenylatlon signal. We have now defined several...
Clinton C. Macdonald, Jeffrey Wilusz, Thomas Shenk
The CstF polyadenylation factor is a multisubunit complex required for efficient cleavage and polyadenylation ofpre-mRNAs. UsinganRNase H-mediated mapping technique, we show that the 64-kDa subunit...
GRSF-1: a poly(A)+ mRNA binding protein which interacts with a conserved G-rich element (1994)
Computer predictions identified similarities to a 14-base G-rich element in numerous mRNAs at a variety of locations. A Northwestern screening strategy was used to obtain a cDNA clone from a HeLa...
Chou, Zhi-Fang, Chen, Fan, Wilusz, Jeffrey
We have defined the positional and sequence requirements of U-rich downstream elements using a simian virus 40 late polyadenylation signal containing a substituted downstream region. A UUUUU element...
Park, Young Woo, Wilusz, Jeffrey, Katze, Michael G.
To better understand regulation of eukaryotic protein synthesis, we studied cellular and viral mRNA translation in influenza virus-infected cells. Influenza virus infection results in a dramatic...
Veraldi, Kristen L., Arhin, George K., Martincic, Kathleen, Chung-Ganster, Ling-Hsiu, Wilusz, Jeffrey, Milcarek, Christine
Previous studies on the regulation of polyadenylation of the immunoglobulin (Ig) heavy-chain pre-mRNA argued for trans-acting modifiers of the cleavage-polyadenylation reaction operating...
Arhin, George K., Boots, Monika, Bagga, Paramjeet S., Milcarek, Christine, Wilusz, Jeffrey
Auxiliary factors likely play an important role in determining the polyadenylation efficiency of mammalian pre-mRNAs. We previously identified an auxiliary factor, hnRNP H/H′, which stimulates...
The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements
Mukherjee, Devi, Gao, Min, O’Connor, J.Patrick, Raijmakers, Reinout, Pruijn, Ger, Lutz, Carol S., ...
HeLa cytoplasmic extracts contain both 3′–5′ and 5′–3′ exonuclease activities that may play important roles in mRNA decay. Using an in vitro RNA deadenylation/decay assay, mRNA decay...
Kash, John C., Cunningham, Dawn M., Smit, Maria W., Park, Youngwoo, Fritz, David, Wilusz, Jeffrey, ...
To understand the regulation of cap-dependent translation initiation mediated by specific 5′ untranslated region (UTR) RNA-protein interactions in mammalian cells, we have studied the selective...
Milone, Joseph, Wilusz, Jeffrey, Bellofatto, Vivian
mRNA turnover is a regulated process that contributes to the steady state level of cytoplasmic mRNA. The amount of each mRNA determines, to a large extent, the amount of protein produced by that...
A novel mRNA-decapping activity in HeLa cytoplasmic extracts is regulated by AU-rich elements
Gao, Min, Wilusz, Carol J., Peltz, Stuart W., Wilusz, Jeffrey
While decapping plays a major role in mRNA turnover in yeast, biochemical evidence for a similar activity in mammalian cells has been elusive. We have now identified a decapping activity in HeLa...
Ford, Lance P., Watson, Janice, Keene, Jack D., Wilusz, Jeffrey
We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition...
Seal, Ruth, Temperley, Richard, Wilusz, Jeffrey, Lightowlers, Robert N.
PARN, a poly(A)-specific ribonuclease, binds the 5′ cap-structure of mRNA and initiates deadenylation-dependent decay. Eukaryotic initiation factor 4E (eIF4E) also binds to the cap structure, an...
Sachchithananthan, Mythily, Stasinopoulos, Stan J., Wilusz, Jeffrey, Medcalf, Robert L.
The human prothrombin G20210A polymorphism located at the 3′ cleavage site of the mRNA results in elevated plasma prothrombin levels and increased risk of venous thrombosis. This polymorphism has...
Analysis of the products of mRNA decapping and 3'-to-5' decay by denaturing gel electrophoresis.
Bergman, Naomi, Opyrchal, Mateusz, Bates, Elizabeth J, Wilusz, Jeffrey
The majority of mRNA turnover is mediated either by mRNA decapping/5'-to-3' decay or exosome-mediated 3'-to-5' exonucleolytic decay. Current assays to assess mRNA decapping in vitro using cap-labeled...
MILONE, JOSEPH, WILUSZ, JEFFREY, BELLOFATTO, VIVIAN
The stability of mRNAs is an important point in the regulation of gene expression in eukaryotes. The mRNA turnover pathways have been identified in yeast and mammals. However, mRNA turnover pathways...
HU, JUN, LUTZ, CAROL S., WILUSZ, JEFFREY, TIAN, BIN
Polyadenylation is an essential step for the maturation of almost all cellular mRNAs in eukaryotes. In human cells, most poly(A) sites are flanked by the upstream AAUAAA hexamer or a close variant,...
CUG-BP binds to RNA substrates and recruits PARN deadenylase
Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
CUG-BP is the human homolog of the Xenopus EDEN-BP, which was shown previously to bind to mRNAs, such as c-mos, that exhibit rapid deadenylation following fertilization of the oocyte. While several...
Park, Young Woo, Wilusz, Jeffrey, Katze, Michael G.
To better understand regulation of eukaryotic protein synthesis, we studied cellular and viral mRNA translation in influenza virus-infected cells. Influenza virus infection results in a dramatic...
Veraldi, Kristen L., Arhin, George K., Martincic, Kathleen, Chung-Ganster, Ling-Hsiu, Wilusz, Jeffrey, Milcarek, Christine
Previous studies on the regulation of polyadenylation of the immunoglobulin (Ig) heavy-chain pre-mRNA argued for trans-acting modifiers of the cleavage-polyadenylation reaction operating...
Arhin, George K., Boots, Monika, Bagga, Paramjeet S., Milcarek, Christine, Wilusz, Jeffrey
Auxiliary factors likely play an important role in determining the polyadenylation efficiency of mammalian pre-mRNAs. We previously identified an auxiliary factor, hnRNP H/H′, which stimulates...
The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements
Mukherjee, Devi, Gao, Min, O’Connor, J.Patrick, Raijmakers, Reinout, Pruijn, Ger, Lutz, Carol S., ...
HeLa cytoplasmic extracts contain both 3′–5′ and 5′–3′ exonuclease activities that may play important roles in mRNA decay. Using an in vitro RNA deadenylation/decay assay, mRNA decay...
Kash, John C., Cunningham, Dawn M., Smit, Maria W., Park, Youngwoo, Fritz, David, Wilusz, Jeffrey, ...
To understand the regulation of cap-dependent translation initiation mediated by specific 5′ untranslated region (UTR) RNA-protein interactions in mammalian cells, we have studied the selective...
Milone, Joseph, Wilusz, Jeffrey, Bellofatto, Vivian
mRNA turnover is a regulated process that contributes to the steady state level of cytoplasmic mRNA. The amount of each mRNA determines, to a large extent, the amount of protein produced by that...
A novel mRNA-decapping activity in HeLa cytoplasmic extracts is regulated by AU-rich elements
Gao, Min, Wilusz, Carol J., Peltz, Stuart W., Wilusz, Jeffrey
While decapping plays a major role in mRNA turnover in yeast, biochemical evidence for a similar activity in mammalian cells has been elusive. We have now identified a decapping activity in HeLa...
Ford, Lance P., Watson, Janice, Keene, Jack D., Wilusz, Jeffrey
We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition...
Seal, Ruth, Temperley, Richard, Wilusz, Jeffrey, Lightowlers, Robert N.
PARN, a poly(A)-specific ribonuclease, binds the 5′ cap-structure of mRNA and initiates deadenylation-dependent decay. Eukaryotic initiation factor 4E (eIF4E) also binds to the cap structure, an...
Sachchithananthan, Mythily, Stasinopoulos, Stan J., Wilusz, Jeffrey, Medcalf, Robert L.
The human prothrombin G20210A polymorphism located at the 3′ cleavage site of the mRNA results in elevated plasma prothrombin levels and increased risk of venous thrombosis. This polymorphism has...
Analysis of the products of mRNA decapping and 3'-to-5' decay by denaturing gel electrophoresis.
Bergman, Naomi, Opyrchal, Mateusz, Bates, Elizabeth J, Wilusz, Jeffrey
The majority of mRNA turnover is mediated either by mRNA decapping/5'-to-3' decay or exosome-mediated 3'-to-5' exonucleolytic decay. Current assays to assess mRNA decapping in vitro using cap-labeled...
HU, JUN, LUTZ, CAROL S., WILUSZ, JEFFREY, TIAN, BIN
Polyadenylation is an essential step for the maturation of almost all cellular mRNAs in eukaryotes. In human cells, most poly(A) sites are flanked by the upstream AAUAAA hexamer or a close variant,...
MILONE, JOSEPH, WILUSZ, JEFFREY, BELLOFATTO, VIVIAN
The stability of mRNAs is an important point in the regulation of gene expression in eukaryotes. The mRNA turnover pathways have been identified in yeast and mammals. However, mRNA turnover pathways...
CUG-BP binds to RNA substrates and recruits PARN deadenylase
Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
CUG-BP is the human homolog of the Xenopus EDEN-BP, which was shown previously to bind to mRNAs, such as c-mos, that exhibit rapid deadenylation following fertilization of the oocyte. While several...
Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome
Anderson, John R., Mukherjee, Devi, Muthukumaraswamy, Karthika, Moraes, Karen C.M., Wilusz, Carol J., Wilusz, Jeffrey
We have previously demonstrated that PM-Scl-75, a component of the human exosome complex involved in RNA maturation and mRNA decay, can specifically interact with RNAs containing an AU-rich...
Efficient In Vitro Amplification of Chronic Wasting Disease PrPRES▿
Kurt, Timothy D., Perrott, Matthew R., Wilusz, Carol J., Wilusz, Jeffrey, Supattapone, Surachai, Telling, Glenn C., ...
Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrPC, to a protease-resistant conformer, PrPCWD. Here we report the use of both...
Aedes aegypti uses RNA interference in defense against Sindbis virus infection
Campbell, Corey L, Keene, Kimberly M, Brackney, Douglas E, Olson, Ken E, Blair, Carol D, Wilusz, Jeffrey, ...
Garneau, Nicole L., Sokoloski, Kevin J., Opyrchal, Mateusz, Neff, C. Preston, Wilusz, Carol J., Wilusz, Jeffrey
The positive-sense transcripts of Sindbis virus (SINV) resemble cellular mRNAs in that they possess a 5′ cap and a 3′ poly(A) tail. It is likely, therefore, that SINV RNAs must successfully...
Sánchez-Vargas, Irma, Scott, Jaclyn C., Poole-Smith, B. Katherine, Franz, Alexander W. E., Barbosa-Solomieu, Valérie, Wilusz, Jeffrey, ...
A number of studies have shown that both innate and adaptive immune defense mechanisms greatly influence the course of human dengue virus (DENV) infections, but little is known about the innate...
Zhang, Libin, Lee, Jerome E., Wilusz, Jeffrey, Wilusz, Carol J.
Type I myotonic dystrophy (DM1) is caused by a triplet repeat expansion in the 3′-untranslated region (UTR) of the dystrophia myotonia protein kinase (DMPK) gene. Pathogenesis is closely linked...