Jens S. Andersen, Bernhard Küster, Re Podtelejnikov, Ejvind Mørtz, Matthias Mann
Nanoelectrospray tandem mass spectrometry has demonstrated its capability for high sensitivity and high throughput characterization of gel-separated proteins (1). Only minimal amounts of protein are...
Roberto Gherzi, Michele Trabucchi, Marco Ponassi, Tina Ruggiero, Giorgio Corte, Christoph Moroni, ...
β-catenin plays an essential role in several biological events including cell fate determination, cell proliferation, and transformation. Here we report that β-catenin is encoded by a labile...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture (2006)
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture (2006)
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture (2006)
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture (2006)
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
NOPdb: Nucleolar Proteome Database (2006)
Leung, Anthony Kar Lun, Trinkle-Mulcahy, Laura, Lam, Yun Wah, Andersen, Jens S., Mann, Matthias, Lamond, Angus I.
The Nucleolar Proteome Database (NOPdb) archives data on >700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture (2006)
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture (2006)
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture (2006)
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
doi:10.1093/nar/gkj004 NOPdb: Nucleolar Proteome Database (2005)
Anthony Kar, Lun Leung, Laura Trinkle-mulcahy, Yun Wah Lam, Jens S. Andersen, Matthias Mann, ...
The Nucleolar Proteome Database (NOPdb) archives data on.700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most...
Amit, Sharon, Hatzubai, Ada, Birman, Yaara, Andersen, Jens S., Ben-Shushan, Etti, Mann, Matthias, ...
Pseudosubstrate regulation of the SCFbeta -TrCP ubiquitin ligase by hnRNP-U (2002)
Davis, Matti, Hatzubai, Ada, Andersen, Jens S., Ben-Shushan, Etti, Fisher, Gregory Zvi, Yaron, Avraham, ...
Chen, Ching-Yi, Gherzi, Roberto, Andersen, Jens S., Gaietta, Guido, Jürchott, Karsten, Royer, Hans-Dieter, ...
Vytvytska, Oresta, Jakobsen, Jimmy S., Balcunaite, Gina, Andersen, Jens S., Baccarini, Manuela, Von Gabain, Alexander
The stability of the ompA mRNA depends on the bacterial growth rate. The 5′ untranslated region is the stability determinant of this transcript and the target of the endoribonuclease, RNase E, the...
p70S6 kinase signals cell survival as well as growth, inactivating the pro-apoptotic molecule BAD
Harada, Hisashi, Andersen, Jens S., Mann, Matthias, Terada, Naohiro, Korsmeyer, Stanley J.
Cytokines often deliver simultaneous, yet distinct, cell growth and cell survival signals. The 70-kDa ribosomal protein S6 kinase (p70S6K) is known to regulate cell growth by inducing protein...
The Vtc proteins in vacuole fusion: coupling NSF activity to V0 trans-complex formation
Müller, Oliver, Bayer, Martin J., Peters, Christopher, Andersen, Jens S., Mann, Matthias, Mayer, Andreas
The fusion of cellular membranes comprises several steps; membrane attachment requires priming of SNAREs and tethering factors by Sec18p/NSF (N-ethylmaleimide sensitive factor) and LMA1. This leads...
Axin-mediated CKI phosphorylation of β-catenin at Ser 45: a molecular switch for the Wnt pathway
Amit, Sharon, Hatzubai, Ada, Birman, Yaara, Andersen, Jens S., Ben-Shushan, Etti, Mann, Matthias, ...
The Wnt pathway controls numerous developmental processes via the β-catenin–TCF/LEF transcription complex. Deregulation of the pathway results in the aberrant accumulation of β-catenin in the...
Chen, Ching-Yi, Gherzi, Roberto, Andersen, Jens S., Gaietta, Guido, Jürchott, Karsten, Royer, Hans-Dieter, ...
Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in...
BASP1 Is a Transcriptional Cosuppressor for the Wilms' Tumor Suppressor Protein WT1
Carpenter, Brian, Hill, Kathryn J., Charalambous, Marika, Wagner, Kate J., Lahiri, Diya, James, Dominic I., ...
The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation...
Bioinformatic analysis of the nucleolus.
Leung, Anthony K L, Andersen, Jens S, Mann, Matthias, Lamond, Angus I
The nucleolus is a plurifunctional, nuclear organelle, which is responsible for ribosome biogenesis and many other functions in eukaryotes, including RNA processing, viral replication and tumour...
NOPdb: Nucleolar Proteome Database
Leung, Anthony Kar Lun, Trinkle-Mulcahy, Laura, Lam, Yun Wah, Andersen, Jens S., Mann, Matthias, Lamond, Angus I.
The Nucleolar Proteome Database (NOPdb) archives data on >700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most...
Pseudosubstrate regulation of the SCFβ-TrCP ubiquitin ligase by hnRNP-U
Davis, Matti, Hatzubai, Ada, Andersen, Jens S., Ben-Shushan, Etti, Fisher, Gregory Zvi, Yaron, Avraham, ...
β-TrCP/E3RS (E3RS) is the F-box protein that functions as the receptor subunit of the SCFβ-TrCP ubiquitin ligase (E3). Surprisingly, although its two recognized substrates, IκBα and β-catenin,...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
Vytvytska, Oresta, Jakobsen, Jimmy S., Balcunaite, Gina, Andersen, Jens S., Baccarini, Manuela, Von Gabain, Alexander
The stability of the ompA mRNA depends on the bacterial growth rate. The 5′ untranslated region is the stability determinant of this transcript and the target of the endoribonuclease, RNase E, the...
p70S6 kinase signals cell survival as well as growth, inactivating the pro-apoptotic molecule BAD
Harada, Hisashi, Andersen, Jens S., Mann, Matthias, Terada, Naohiro, Korsmeyer, Stanley J.
Cytokines often deliver simultaneous, yet distinct, cell growth and cell survival signals. The 70-kDa ribosomal protein S6 kinase (p70S6K) is known to regulate cell growth by inducing protein...
The Vtc proteins in vacuole fusion: coupling NSF activity to V0 trans-complex formation
Müller, Oliver, Bayer, Martin J., Peters, Christopher, Andersen, Jens S., Mann, Matthias, Mayer, Andreas
The fusion of cellular membranes comprises several steps; membrane attachment requires priming of SNAREs and tethering factors by Sec18p/NSF (N-ethylmaleimide sensitive factor) and LMA1. This leads...
Pseudosubstrate regulation of the SCFβ-TrCP ubiquitin ligase by hnRNP-U
Davis, Matti, Hatzubai, Ada, Andersen, Jens S., Ben-Shushan, Etti, Fisher, Gregory Zvi, Yaron, Avraham, ...
β-TrCP/E3RS (E3RS) is the F-box protein that functions as the receptor subunit of the SCFβ-TrCP ubiquitin ligase (E3). Surprisingly, although its two recognized substrates, IκBα and β-catenin,...
Axin-mediated CKI phosphorylation of β-catenin at Ser 45: a molecular switch for the Wnt pathway
Amit, Sharon, Hatzubai, Ada, Birman, Yaara, Andersen, Jens S., Ben-Shushan, Etti, Mann, Matthias, ...
The Wnt pathway controls numerous developmental processes via the β-catenin–TCF/LEF transcription complex. Deregulation of the pathway results in the aberrant accumulation of β-catenin in the...
Chen, Ching-Yi, Gherzi, Roberto, Andersen, Jens S., Gaietta, Guido, Jürchott, Karsten, Royer, Hans-Dieter, ...
Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in...
BASP1 Is a Transcriptional Cosuppressor for the Wilms' Tumor Suppressor Protein WT1
Carpenter, Brian, Hill, Kathryn J., Charalambous, Marika, Wagner, Kate J., Lahiri, Diya, James, Dominic I., ...
The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation...
Bioinformatic analysis of the nucleolus.
Leung, Anthony K L, Andersen, Jens S, Mann, Matthias, Lamond, Angus I
The nucleolus is a plurifunctional, nuclear organelle, which is responsible for ribosome biogenesis and many other functions in eukaryotes, including RNA processing, viral replication and tumour...
NOPdb: Nucleolar Proteome Database
Leung, Anthony Kar Lun, Trinkle-Mulcahy, Laura, Lam, Yun Wah, Andersen, Jens S., Mann, Matthias, Lamond, Angus I.
The Nucleolar Proteome Database (NOPdb) archives data on >700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most...
Identification of miRNA targets with stable isotope labeling by amino acids in cell culture
Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell...
Schwer, Bjoern, Bunkenborg, Jakob, Verdin, Regis O., Andersen, Jens S., Verdin, Eric
We report that human acetyl-CoA synthetase 2 (AceCS2) is a mitochondrial matrix protein. AceCS2 is reversibly acetylated at Lys-642 in the active site of the enzyme. The mitochondrial sirtuin SIRT3...
Gherzi, Roberto, Trabucchi, Michele, Ponassi, Marco, Ruggiero, Tina, Corte, Giorgio, Moroni, Christoph, ...
β-catenin plays an essential role in several biological events including cell fate determination, cell proliferation, and transformation. Here we report that β-catenin is encoded by a labile...
Analysis of Nucleolar Protein Dynamics Reveals the Nuclear Degradation of Ribosomal Proteins
Lam, Yun Wah, Lamond, Angus I., Mann, Matthias, Andersen, Jens S.
Organellar proteomics: turning inventories into insights
Andersen, Jens S, Mann, Matthias
Subcellular organization is yielding to large-scale analysis. Researchers are now applying robust mass-spectrometry-based proteomics methods to obtain an inventory of biochemically isolated...
Bod1, a novel kinetochore protein required for chromosome biorientation
Porter, Iain M., McClelland, Sarah E., Khoudoli, Guennadi A., Hunter, Christopher J., Andersen, Jens S., McAinsh, Andrew D., ...
We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper...
Cardoso, Carla M. P., Groth-Pedersen, Line, Høyer-Hansen, Maria, Kirkegaard, Thomas, Corcelle, Elizabeth, Andersen, Jens S., ...
FACT facilitates chromatin transcription by RNA polymerases I and III
Birch, Joanna L, Tan, Bertrand C-M, Panov, Kostya I, Panova, Tatiana B, Andersen, Jens S, Owen-Hughes, Tom A, ...
Efficient transcription elongation from a chromatin template requires RNA polymerases (Pols) to negotiate nucleosomes. Our biochemical analyses demonstrate that RNA Pol I can transcribe through...