Jeremy C. Mottram

Studies on the CPA cysteine peptidase in the Leishmania infantumgenome strain JPCM5 (2006)

Denise, Hubert, Poot, Jacqueline, Jiménez, Maribel, Ambit, Audrey, Herrmann, Daland C, Vermeulen, Arno N, ...

Abstract Background Visceral leishmaniasis caused by members of the Leishmania donovani complex is often fatal in the absence of treatment. Research has been hampered by the lack of good laboratory...

The SNARE protein family of Leishmania major (2006)

Besteiro, Sébastien, Coombs, Graham H, Mottram, Jeremy C

Abstract Background Leishmania major is a protozoan parasite with a highly polarised cell shape that depends upon endocytosis and exocytosis from a single area of the plasma membrane, the flagellar...

Comparative analysis of the kinomes of three pathogenic trypanosomatids: Leishmania major, Trypanosoma bruceiand Trypanosoma cruzi (2005)

Parsons, Marilyn, Worthey, Elizabeth A, Ward, Pauline N, Mottram, Jeremy C

Abstract Background The trypanosomatids Leishmania major , Trypanosoma brucei and Trypanosoma cruzi cause some of the most debilitating diseases of humankind: cutaneous leishmaniasis, African...

Inhibitors of Leishmania mexicana CRK3 Cyclin-Dependent Kinase: Chemical Library Screen and Antileishmanial Activity. (2004)

Grant, Karen M., Dunion, Morag H., Yardley, Vanessa, Skaltsounis , Alexios-Leandros, Marko, Doris, Eisenbrand, Gerhard, ...

The CRK3 cyclin-dependent kinase of Leishmania has been shown by genetic manipulation of the parasite to be essential for proliferation. We present data which demonstrate that chemical inhibition of...

Intracellular targets of paullones : identification by affinity chromatography using immobilized inhibitor. (2002)

Knockaert, Marie, Wieking, Karen, Schmitt, Sophie, Leost, Maryse, Grant, Karen M., Mottram, Jeremy C., ...

Numerous inhibitors of cyclin-dependent kinases and glycogen synthase kinase-3 (GSK-3) are being developed in view of their potential applications against cancers and neurodegenerative disorders....

The CRK3 protein kinase is essential for cell cycle progression of Leishmania mexicana. (2001)

Hassan, Paul, Fergusson, David, Grant, Karen M., Mottram, Jeremy C.

The Leishmania mexicana CRK3 gene encodes a cdc2-related protein kinase with activity towards histone H1. Attempts to disrupt both alleles of CRK3 in the promastigote life-cycle stage resulted in...

Intracellular targets of cyclin-dependent kinase inhibitors: identification by affinity chromatography using immobilised inhibitors. (2000)

Knockaert, Marie, Gray, N., Damiens, E., Grellier, P., Grant, Karen M., ...

Background: Chemical inhibitors of cyclin-dependent kinases (CDKs) have great therapeutic potential against various proliferative and neurodegenerative disorders. Olomoucine, a 2,6,9-trisubstituted...

Leishmania mexicana Mutants Lacking Glycosylphosphatidylinositol (GPI):Protein Transamidase Provide Insights into the Biosynthesis and Functions of GPI-anchored Proteins

Hilley, James D., Zawadzki, Jody L., McConville, Malcolm J., Coombs, Graham H., Mottram, Jeremy C.

The major surface proteins of the parasitic protozoon Leishmania mexicana are anchored to the plasma membrane by glycosylphosphatidylinositol (GPI) anchors. We have cloned the L. mexicana GPI8 gene...

Trifluoromethionine, a Prodrug Designed against Methionine γ-Lyase-Containing Pathogens, Has Efficacy In Vitro and In Vivo against Trichomonas vaginalis

Coombs, Graham H., Mottram, Jeremy C.

Methionine γ-lyase, the enzyme which catalyzes the single-step conversion of methionine to α-ketobutyrate, ammonia, and methanethiol, is highly active in many anaerobic pathogenic microorganisms...

Essential Roles for GPI-anchored Proteins in African Trypanosomes Revealed Using Mutants Deficient in GPI8

Lillico, Simon, Field, Mark C., Blundell, Pat, Coombs, Graham H., Mottram, Jeremy C.

The survival of Trypanosoma brucei, the causative agent of Sleeping Sickness and Nagana, is facilitated by the expression of a dense surface coat of glycosylphosphatidylinositol (GPI)-anchored...

Expression of Multiple CPB Genes Encoding Cysteine Proteases Is Required for Leishmania mexicana Virulence In Vivo

Denise, Hubert, McNeil, Kathryn, Brooks, Darren R., Alexander, James, Coombs, Graham H., Mottram, Jeremy C.

Leishmania mexicana mutants deficient in the multicopy CPB gene array have reduced virulence, demonstrated by poor lesion growth in BALB/c mice and induction of a protective Th1 response. Reinsertion...

Inhibitors of Leishmania mexicana CRK3 Cyclin-Dependent Kinase: Chemical Library Screen and Antileishmanial Activity

Grant, Karen M., Dunion, Morag H., Yardley, Vanessa, Skaltsounis, Alexios-Leandros, Marko, Doris, Eisenbrand, Gerhard, ...

The CRK3 cyclin-dependent kinase of Leishmania has been shown by genetic manipulation of the parasite to be essential for proliferation. We present data which demonstrate that chemical inhibition of...

Novel peptide inhibitors of Leishmania gp63 based on the cleavage site of MARCKS (myristoylated alanine-rich C kinase substrate)-related protein.

Corradin, Sally, Ransijn, Adriana, Corradin, Giampietro, Bouvier, Jacques, Delgado, Maria Belen, Fernandez-Carneado, Jimena, ...

The zinc metalloprotease gp63 (leishmanolysin; promastigote surface protease) is expressed at high density at the surface of Leishmania promastigotes. Efficient non-toxic inhibitors of gp63 do not...

THE STRUCTURE OF LEISHMANIA MEXICANA ICP PROVIDES EVIDENCE FOR CONVERGENT EVOLUTION OF CYSTEINE PEPTIDASE INHIBITORS*

Smith, Brian O., Picken, Nichola C., Westrop, Gareth D., Bromek, Krystyna, Mottram, Jeremy C., Coombs, Graham H.

Clan CA, family C1 cysteine peptidases (CPs) are important virulence factors and drug targets in parasites that cause neglected diseases. Natural CP inhibitors of the I42 family, known as ICP, occur...

Leishmania mexicana Mutants Lacking Glycosylphosphatidylinositol (GPI):Protein Transamidase Provide Insights into the Biosynthesis and Functions of GPI-anchored Proteins

Hilley, James D., Zawadzki, Jody L., McConville, Malcolm J., Coombs, Graham H., Mottram, Jeremy C.

The major surface proteins of the parasitic protozoon Leishmania mexicana are anchored to the plasma membrane by glycosylphosphatidylinositol (GPI) anchors. We have cloned the L. mexicana GPI8 gene...

Trifluoromethionine, a Prodrug Designed against Methionine γ-Lyase-Containing Pathogens, Has Efficacy In Vitro and In Vivo against Trichomonas vaginalis

Coombs, Graham H., Mottram, Jeremy C.

Methionine γ-lyase, the enzyme which catalyzes the single-step conversion of methionine to α-ketobutyrate, ammonia, and methanethiol, is highly active in many anaerobic pathogenic microorganisms...

Essential Roles for GPI-anchored Proteins in African Trypanosomes Revealed Using Mutants Deficient in GPI8

Lillico, Simon, Field, Mark C., Blundell, Pat, Coombs, Graham H., Mottram, Jeremy C.

The survival of Trypanosoma brucei, the causative agent of Sleeping Sickness and Nagana, is facilitated by the expression of a dense surface coat of glycosylphosphatidylinositol (GPI)-anchored...

Expression of Multiple CPB Genes Encoding Cysteine Proteases Is Required for Leishmania mexicana Virulence In Vivo

Denise, Hubert, McNeil, Kathryn, Brooks, Darren R., Alexander, James, Coombs, Graham H., Mottram, Jeremy C.

Leishmania mexicana mutants deficient in the multicopy CPB gene array have reduced virulence, demonstrated by poor lesion growth in BALB/c mice and induction of a protective Th1 response. Reinsertion...

Inhibitors of Leishmania mexicana CRK3 Cyclin-Dependent Kinase: Chemical Library Screen and Antileishmanial Activity

Grant, Karen M., Dunion, Morag H., Yardley, Vanessa, Skaltsounis, Alexios-Leandros, Marko, Doris, Eisenbrand, Gerhard, ...

The CRK3 cyclin-dependent kinase of Leishmania has been shown by genetic manipulation of the parasite to be essential for proliferation. We present data which demonstrate that chemical inhibition of...

Novel peptide inhibitors of Leishmania gp63 based on the cleavage site of MARCKS (myristoylated alanine-rich C kinase substrate)-related protein.

Corradin, Sally, Ransijn, Adriana, Corradin, Giampietro, Bouvier, Jacques, Delgado, Maria Belen, Fernandez-Carneado, Jimena, ...

The zinc metalloprotease gp63 (leishmanolysin; promastigote surface protease) is expressed at high density at the surface of Leishmania promastigotes. Efficient non-toxic inhibitors of gp63 do not...

Trypanosoma brucei Polo-like kinase is essential for basal body duplication, kDNA segregation and cytokinesis

Hammarton, Tansy C, Kramer, Susanne, Tetley, Laurence, Boshart, Michael, Mottram, Jeremy C

Polo-like kinases (PLKs) are conserved eukaryotic cell cycle regulators, which play multiple roles, particularly during mitosis. The function of Trypanosoma brucei PLK was investigated in procyclic...