Jesús Prieto

Characterization of the human Nα-terminal acetyltransferase B enzymatic complex (2009)

Ametzazurra, Amagoia, Gázquez, Cristina, Lasa, Marta, Larrea, Esther, Prieto, Jesús, Aldabe, Rafael

Abstract Background Human N α -acetyltransferase complex B (hNatB) is integrated by hNaa20p (hNAT5/hNAT3) and hNaa25p (hMDM20) proteins. Previous data have shown that this enzymatic complex is...

Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease (2009)

Grizot, Sylvestre, Smith, Julianne, Daboussi, Fayza, Prieto, Jesús, Redondo, Pilar, Merino, Nekane, ...

Sequence-specific endonucleases recognizing long target sequences are emerging as powerful tools for genome engineering. These endonucleases could be used to correct deleterious mutations or to...

The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage (2007)

Prieto, Jesús, Redondo, Pilar, Padró, Daniel, Arnould, Sylvain, Epinat, Jean-Charles, Pâques, Frédéric, ...

Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel...

Engineering Th determinants for efficient priming of humoral and cytotoxic T cell responses (2003)

Lasarte, Juan José, Casares, Noelia, Sarobe, Pablo, Ruiz, Marta, Prieto, Jesús, ...

To engineer Th determinants (THd) to prime help for humoral or cytotoxic T cell responses, we modified ovalbumin [OVA323–337] and myoglobin [MYO106–118] eliciting Th1 and Th0...

Th1 but not Th0 cell help is efficient to induce cytotoxic T lymphocytes by immunization with short synthetic peptides (1999)

Casares, Noelia, Lasarte, Juan José, Sarobe, Pablo, Pérez-Mediavilla, Luis Alberto, Ruiz, Marta, ...

Immunization of BALB/c mice with peptide HVSGHRMAWDMMMNWA, encompassing residues 121–135 from hepatitis C virus E1 protein, induced CD4+ Th1 cells as well as a long-lasting CD8+ cytotoxic...

Abnormal Priming of CD4+ T Cells by Dendritic Cells Expressing Hepatitis C Virus Core and E1 Proteins

Sarobe, Pablo, Lasarte, Juan José, Casares, Noelia, Baixeras, Elena, Labarga, Pablo, ...

Patients infected with hepatitis C virus (HCV) have an impaired response against HCV antigens while keeping immune competence for other antigens. We hypothesized that expression of HCV proteins in...

Inhibiting expression of specific genes in mammalian cells with 5′ end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA

Fortes, Puri, Cuevas, Yolanda, Guan, Fei, Liu, Peng, Pentlicky, Sara, Jung, Stephen P., ...

Reducing or eliminating expression of a given gene is likely to require multiple methods to ensure coverage of all of the genes in a given mammalian cell. We and others [Furth, P. A., Choe, W. T.,...

Hepatitis C Virus Structural Proteins Impair Dendritic Cell Maturation and Inhibit In Vivo Induction of Cellular Immune Responses

Sarobe, Pablo, Lasarte, Juan José, Zabaleta, Aintzane, Arribillaga, Laura, Arina, Ainhoa, Melero, Ignacio, ...

Hepatitis C virus (HCV) chronic infection is characterized by low or undetectable cellular immune responses against HCV antigens. Some studies have suggested that HCV proteins manipulate the immune...

Anion exchanger 2 is essential for spermiogenesis in mice

Medina, Juan F., Recalde, Sergio, Prieto, Jesús, Lecanda, Jon, Sáez, Elena, Funk, Colin D., ...

Na+-independent anion exchangers (AE) mediate electroneutral exchange of Cl- for \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb}...

Abnormal Priming of CD4+ T Cells by Dendritic Cells Expressing Hepatitis C Virus Core and E1 Proteins

Sarobe, Pablo, Lasarte, Juan José, Casares, Noelia, Baixeras, Elena, Labarga, Pablo, ...

Patients infected with hepatitis C virus (HCV) have an impaired response against HCV antigens while keeping immune competence for other antigens. We hypothesized that expression of HCV proteins in...

Inhibiting expression of specific genes in mammalian cells with 5′ end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA

Fortes, Puri, Cuevas, Yolanda, Guan, Fei, Liu, Peng, Pentlicky, Sara, Jung, Stephen P., ...

Reducing or eliminating expression of a given gene is likely to require multiple methods to ensure coverage of all of the genes in a given mammalian cell. We and others [Furth, P. A., Choe, W. T.,...

Hepatitis C Virus Structural Proteins Impair Dendritic Cell Maturation and Inhibit In Vivo Induction of Cellular Immune Responses

Sarobe, Pablo, Lasarte, Juan José, Zabaleta, Aintzane, Arribillaga, Laura, Arina, Ainhoa, Melero, Ignacio, ...

Hepatitis C virus (HCV) chronic infection is characterized by low or undetectable cellular immune responses against HCV antigens. Some studies have suggested that HCV proteins manipulate the immune...

Anion exchanger 2 is essential for spermiogenesis in mice

Medina, Juan F., Recalde, Sergio, Prieto, Jesús, Lecanda, Jon, Sáez, Elena, Funk, Colin D., ...

Na+-independent anion exchangers (AE) mediate electroneutral exchange of Cl- for \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb}...

A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences

Smith, Julianne, Grizot, Sylvestre, Arnould, Sylvain, Duclert, Aymeric, Epinat, Jean-Charles, Chames, Patrick, ...

Meganucleases, or homing endonucleases (HEs) are sequence-specific endonucleases with large (>14 bp) cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells...

Inefficient Chronic Activation of Parietal Cells in Ae2a,b−/− Mice

Recalde, Sergio, Muruzábal, Francisco, Looije, Norbert, Kunne, Cindy, Burrell, María A., Sáez, Elena, ...

In parietal cells, basolateral Ae2 Cl−/HCO3− exchanger (Slc4a2) appears to compensate for luminal H+ pumping while providing Cl− for apical secretion. In mouse and rat, mRNA variants Ae2a,...

Effect of Adenovirus-Mediated RNA Interference on Endogenous MicroRNAs in a Mouse Model of Multidrug Resistance Protein 2 Gene Silencing▿

Narvaiza, Iñigo, Aparicio, Oscar, Vera, María, Razquin, Nerea, Bortolanza, Sergia, Prieto, Jesús, ...

RNA interference with viral vectors that express short hairpin RNAs (shRNAs) has emerged as a powerful tool for functional genomics and therapeutic purposes. However, little is known about shRNA in...

The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage

Prieto, Jesús, Redondo, Pilar, Padró, Daniel, Arnould, Sylvain, Epinat, Jean-Charles, Pâques, Frédéric, ...

Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel...

Upregulation of Indoleamine 2,3-Dioxygenase in Hepatitis C Virus Infection▿

Larrea, Esther, Riezu-Boj, José I., Gil-Guerrero, Lucía, Casares, Noelia, Aldabe, Rafael, Sarobe, Pablo, ...

Indoleamine 2,3-dioxygenase (IDO) is induced by proinflammatory cytokines and by CTLA-4-expressing T cells and constitutes an important mediator of peripheral immune tolerance. In chronic hepatitis...

Cardiotrophin-1 defends the liver against ischemia-reperfusion injury and mediates the protective effect of ischemic preconditioning

Iñiguez, Maria, Berasain, Carmen, Martinez-Ansó, Eduardo, Bustos, Matilde, Fortes, Puri, Pennica, Diane, ...

Ischemia-reperfusion (I/R) liver injury occurs when blood flow is restored after prolonged ischemia. A short interruption of blood flow (ischemic preconditioning [IP]) induces tolerance to subsequent...

Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells

Arenas, Fabián, Hervias, Isabel, Úriz, Miriam, Joplin, Ruth, Prieto, Jesús, Medina, Juan F.

Primary biliary cirrhosis (PBC) is a cholestatic disease associated with autoimmune phenomena and alterations in both biliary bicarbonate excretion and expression of the bicarbonate carrier AE2. The...

Crystal structure of I-DmoI in complex with its target DNA provides new insights into meganuclease engineering

Marcaida, María José, Prieto, Jesús, Redondo, Pilar, Nadra, Alejandro D., Alibés, Andreu, Serrano, Luis, ...

Homing endonucleases, also known as meganucleases, are sequence-specific enzymes with large DNA recognition sites. These enzymes can be used to induce efficient homologous gene targeting in cells and...

Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease

Grizot, Sylvestre, Smith, Julianne, Daboussi, Fayza, Prieto, Jesús, Redondo, Pilar, Merino, Nekane, ...

Sequence-specific endonucleases recognizing long target sequences are emerging as powerful tools for genome engineering. These endonucleases could be used to correct deleterious mutations or to...