Jian-Bing Fan

Evaluation of a new high-dimensional miRNA profiling platform (2009)

Cunningham, Julie M, Oberg, Ann L, Borralho, Pedro M, Kren, Betsy T, French, Amy J, Wang, Liang, ...

Abstract Background MicroRNAs (miRNAs) are a class of approximately 22 nucleotide long, widely expressed RNA molecules that play important regulatory roles in eukaryotes. To investigate miRNA...

Differential Allelic Expression in the Human Genome: A Robust Approach to Identify Genetic and Epigenetic Cis-Acting Mechanisms Regulating Gene Expression (2008)

David Serre, Scott Gurd, Bing Ge, Robert Sladek, Donna Sinnett, Eef Harmsen, ...

The recent development of whole genome association studies has lead to the robust identification of several loci involved in different common human diseases. Interestingly, some of the strongest...

Highly sensitive and specific microRNA expression profiling using BeadArray technology (2008)

Chen, Jing, Lozach, Jean, Garcia, Eliza Wickham, Barnes, Bret, Luo, Shujun, Mikoulitch, Ivan, ...

We have developed a highly sensitive, specific and reproducible method for microRNA (miRNA) expression profiling, using the BeadArray™ technology. This method incorporates an enzyme-assisted...

A second generation human haplotype map of over 3.1 million SNPs (2007)

Frazer, Kelly A., Ballinger, Dennis G., Cox, David R., Hinds, David A., Stuve, Laura L., Gibbs, Richard A., ...

We describe the Phase II HapMap, which characterizes over 3.1 million human single nucleotide polymorphisms (SNPs) genotyped in 270 individuals from four geographically diverse populations and...

Epigenetic Specificity of Loss of Imprinting of the IGF2 Gene in Wilms Tumors (2007)

Bjornsson, Hans T., Brown, Lindsey J., Fallin, M. Danielle, Rongione, Michael A., Bibikova, Marina, Wickham, Eliza, ...

Loss of imprinting (LOI) of the IGF2 gene (which encodes insulin-like growth factor II) is the most common genetic or epigenetic alteration in Wilms tumor; LOI involves aberrant activation of the...

Profiling alternatively spliced mRNA isoforms for prostate cancer classification (2006)

Zhang, Chaolin, Li, Hai-Ri, Fan, Jian-Bing, Wang-Rodriguez, Jessica, Downs, Tracy, Fu, Xiang-Dong, ...

Abstract Background Prostate cancer is one of the leading causes of cancer illness and death among men in the United States and world wide. There is an urgent need to discover good biomarkers for...

High-throughput DNA methylation profiling using universal bead arrays (2006)

Bibikova, Marina, Lin, Zhenwu, Zhou, Lixin, Chudin, Eugene, Garcia, Eliza Wickham, Wu, Bonnie, ...

We have developed a high-throughput method for analyzing the methylation status of hundreds of preselected genes simultaneously and have applied it to the discovery of methylation signatures that...

Human embryonic stem cells have a unique epigenetic signature (2006)

Bibikova, Marina, Chudin, Eugene, Wu, Bonnie, Zhou, Lixin, Garcia, Eliza Wickham, Liu, Ying, ...

Human embryonic stem (hES) cells originate during an embryonic period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We...

High-throughput DNA methylation profiling using universal bead arrays (2006)

Bibikova, Marina, Lin, Zhenwu, Zhou, Lixin, Chudin, Eugene, Garcia, Eliza Wickham, Wu, Bonnie, ...

We have developed a high-throughput method for analyzing the methylation status of hundreds of preselected genes simultaneously and have applied it to the discovery of methylation signatures that...

Human embryonic stem cells have a unique epigenetic signature (2006)

Bibikova, Marina, Chudin, Eugene, Wu, Bonnie, Zhou, Lixin, Wickham Garcia, Eliza, Liu, Ying, ...

Human embryonic stem (hES) cells originate during an embryonic period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We...

Review High-throughput SNP genotyping on universal bead arrays (2004)

Richard Shen, Jian-bing Fan, Derek Campbell, Weihua Chang, Jing Chen, Dennis Doucet, ...

We have developed a flexible, accurate and highly multiplexed SNP genotyping assay for high-throughput genetic analysis of large populations on a bead array platform. The novel genotyping system...

Decoding Randomly Ordered DNA Arrays (2004)

Gunderson, Kevin L., Kruglyak, Semyon, Graige, Michael S., Garcia, Francisco, Kermani, Bahram G., Zhao, Chanfeng, ...

We have developed a simple and efficient algorithm to identify each member of a large collection of DNA-linked objects through the use of hybridization, and have applied it to the manufacture of...

Decoding Randomly Ordered DNA Arrays (2004)

Gunderson, Kevin L., Kruglyak, Semyon, Graige, Michael S., Garcia, Francisco, Kermani, Bahram G., Zhao, Chanfeng, ...

We have developed a simple and efficient algorithm to identify each member of a large collection of DNA-linked objects through the use of hybridization, and have applied it to the manufacture of...

A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices (2004)

Fan, Jian-Bing, Yeakley, Joanne M., Bibikova, Marina, Chudin, Eugene, Wickham, Eliza, Chen, Jing, ...

We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated...

Parallel genotyping of human SNPs using generic high-density oligonucleotide tag arrays. (2000)

Fan, Jian-Bing, Chen, Xiaoqiong, Halushka, Marc K., Berno, Anthony, Huang, Xiaohua, Ryder, Thomas, ...

Large scale human genetic studies require technologies for generating millions of genotypes with relative ease but also at a reasonable cost and with high accuracy. We describe a highly parallel...

Detection and characterization of a ring chromosome in the fission yeast Schizosaccharomyces pombe (1992)

Fan, Jian-Bing, Michel, Rochet, Gaillardin, Claude, Smith, Cassandra L.

Notl and Sfil genomic restriction maps were used to detect and characterize a ring chromosome II in a Schizosaccharomyces pombe strain with a meitotic defect on chromosome II. The ring chromosome was...

Giardia lamblia: haploid genome size determined by pulsed field gel electrophoresis is less than 12 Mb (1991)

Fan, Jian-Bing, Korman, Stanley H., Cantor, Charles R., Smith, Cassandra L.

Previous estimates of the size of the Glardia lambus genome have ranged from 30 to 80 million base pairs (Mb), based on DNA renaturation kinetics. This is much larger than the sum of the sizes of the...

Alignment of Sfi I sites with the Not I restriction map of Schizosaccharomyces pombe genome (1991)

Fan, Jian-Bing, Grothues, Dietmar, Smith, Cassandra L.

A Sfi I restriction map of the fission yeast Schizosaccharomyces pombe genome was aligned with the Not I restriction map. There are 16 Sfi I sites in the S. pombe genome. Three Sfi I sites are on...

Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome (1989)

Fan, Jian-Bing, Chikashige, Yuji, Smith, Cassandra L., Niwa, Osami, Yanagida, Mitsuhiro, Cantor, Charles R.

Pulsed field gel electrophoresis and large DNA technology were used to construct a Not I restriction map of the entire genome of the fission yeast Schizosaccharomyces pombe. There are 14 detectable...

Parallel Genotyping of Human SNPs Using Generic High-density Oligonucleotide Tag Arrays

Fan, Jian-Bing, Chen, Xiaoqiong, Halushka, Marc K., Berno, Anthony, Huang, Xiaohua, Ryder, Thomas, ...

Large scale human genetic studies require technologies for generating millions of genotypes with relative ease but also at a reasonable cost and with high accuracy. We describe a highly parallel...

Expression of an active tobacco mitogen-activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize

Shou, Huixia, Bordallo, Patricia, Fan, Jian-Bing, Yeakley, Joanne M., Bibikova, Marina, Sheen, Jen, ...

Cold acclimation is the major process that prepares plants for freezing tolerance. In addition to extensive transcription regulation by cold-inducible master transcription factors, oxidative stress...

Decoding Randomly Ordered DNA Arrays

Gunderson, Kevin L., Kruglyak, Semyon, Graige, Michael S., Garcia, Francisco, Kermani, Bahram G., Zhao, Chanfeng, ...

We have developed a simple and efficient algorithm to identify each member of a large collection of DNA-linked objects through the use of hybridization, and have applied it to the manufacture of...

A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices

Fan, Jian-Bing, Yeakley, Joanne M., Bibikova, Marina, Chudin, Eugene, Wickham, Eliza, Chen, Jing, ...

We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated...

Salicylic Acid-Dependent Expression of Host Genes in Compatible Arabidopsis-Virus Interactions1[w]

Huang, Zhonglian, Yeakley, Joanne M., Garcia, Elizabeth Wickham, Holdridge, Jaime D., Fan, Jian-Bing, Whitham, Steven A.

Plant viruses elicit the expression of common sets of genes in susceptible hosts. Studies in Arabidopsis (Arabidopsis thaliana) and tomato (Lycopersicon esculentum) indicate that at least one-third...

Functional Analysis of Human Microtubule-based Motor Proteins, the Kinesins and Dyneins, in Mitosis/Cytokinesis Using RNA InterferenceD⃞V⃞

Zhu, Changjun, Zhao, Jian, Bibikova, Marina, Leverson, Joel D., Bossy-Wetzel, Ella, Fan, Jian-Bing, ...

Microtubule (MT)-based motor proteins, kinesins and dyneins, play important roles in multiple cellular processes including cell division. In this study, we describe the generation and use of an...

High-throughput DNA methylation profiling using universal bead arrays

Bibikova, Marina, Lin, Zhenwu, Zhou, Lixin, Chudin, Eugene, Garcia, Eliza Wickham, Wu, Bonnie, ...

We have developed a high-throughput method for analyzing the methylation status of hundreds of preselected genes simultaneously and have applied it to the discovery of methylation signatures that...

Parallel Genotyping of Human SNPs Using Generic High-density Oligonucleotide Tag Arrays

Fan, Jian-Bing, Chen, Xiaoqiong, Halushka, Marc K., Berno, Anthony, Huang, Xiaohua, Ryder, Thomas, ...

Large scale human genetic studies require technologies for generating millions of genotypes with relative ease but also at a reasonable cost and with high accuracy. We describe a highly parallel...

Expression of an active tobacco mitogen-activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize

Shou, Huixia, Bordallo, Patricia, Fan, Jian-Bing, Yeakley, Joanne M., Bibikova, Marina, Sheen, Jen, ...

Cold acclimation is the major process that prepares plants for freezing tolerance. In addition to extensive transcription regulation by cold-inducible master transcription factors, oxidative stress...

Decoding Randomly Ordered DNA Arrays

Gunderson, Kevin L., Kruglyak, Semyon, Graige, Michael S., Garcia, Francisco, Kermani, Bahram G., Zhao, Chanfeng, ...

We have developed a simple and efficient algorithm to identify each member of a large collection of DNA-linked objects through the use of hybridization, and have applied it to the manufacture of...

A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices

Fan, Jian-Bing, Yeakley, Joanne M., Bibikova, Marina, Chudin, Eugene, Wickham, Eliza, Chen, Jing, ...

We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated...

Salicylic Acid-Dependent Expression of Host Genes in Compatible Arabidopsis-Virus Interactions1[w]

Huang, Zhonglian, Yeakley, Joanne M., Garcia, Elizabeth Wickham, Holdridge, Jaime D., Fan, Jian-Bing, Whitham, Steven A.

Plant viruses elicit the expression of common sets of genes in susceptible hosts. Studies in Arabidopsis (Arabidopsis thaliana) and tomato (Lycopersicon esculentum) indicate that at least one-third...

Functional Analysis of Human Microtubule-based Motor Proteins, the Kinesins and Dyneins, in Mitosis/Cytokinesis Using RNA InterferenceD⃞V⃞

Zhu, Changjun, Zhao, Jian, Bibikova, Marina, Leverson, Joel D., Bossy-Wetzel, Ella, Fan, Jian-Bing, ...

Microtubule (MT)-based motor proteins, kinesins and dyneins, play important roles in multiple cellular processes including cell division. In this study, we describe the generation and use of an...

High-throughput DNA methylation profiling using universal bead arrays

Bibikova, Marina, Lin, Zhenwu, Zhou, Lixin, Chudin, Eugene, Garcia, Eliza Wickham, Wu, Bonnie, ...

We have developed a high-throughput method for analyzing the methylation status of hundreds of preselected genes simultaneously and have applied it to the discovery of methylation signatures that...

Human embryonic stem cells have a unique epigenetic signature

Bibikova, Marina, Chudin, Eugene, Wu, Bonnie, Zhou, Lixin, Garcia, Eliza Wickham, Liu, Ying, ...

Human embryonic stem (hES) cells originate during an embryonic period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We...

Quantitative Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using Universal Bead Arrays

Bibikova, Marina, Talantov, Dimitri, Chudin, Eugene, Yeakley, Joanne M., Chen, Jing, Doucet, Dennis, ...

We recently developed a sensitive and flexible gene expression profiling system that is not dependent on an intact poly-A tail and showed that it could be used to analyze degraded RNA samples. We...

Sensitive ChIP-DSL technology reveals an extensive estrogen receptor α-binding program on human gene promoters

Kwon, Young-Soo, Garcia-Bassets, Ivan, Hutt, Kasey R., Cheng, Christine S., Jin, Mingjie, Liu, Dongyan, ...

ChIP coupled with microarray provides a powerful tool to determine in vivo binding profiling of transcription factors to deduce regulatory circuitries in mammalian cells. Aiming at improving the...

Differential Allelic Expression in the Human Genome: A Robust Approach To Identify Genetic and Epigenetic Cis-Acting Mechanisms Regulating Gene Expression

Serre, David, Gurd, Scott, Ge, Bing, Sladek, Robert, Sinnett, Donna, Harmsen, Eef, ...

The recent development of whole genome association studies has lead to the robust identification of several loci involved in different common human diseases. Interestingly, some of the strongest...

Highly sensitive and specific microRNA expression profiling using BeadArray technology

Chen, Jing, Lozach, Jean, Garcia, Eliza Wickham, Barnes, Bret, Luo, Shujun, Mikoulitch, Ivan, ...

We have developed a highly sensitive, specific and reproducible method for microRNA (miRNA) expression profiling, using the BeadArray™ technology. This method incorporates an enzyme-assisted...